Advanced search
Publication Cover
Platelets Volume 11, 2000 - Issue 3
Submit an article Journal homepage
573
Views
68
CrossRef citations to date
0
Altmetric
Research Article

Blood platelet activation evaluated by flow cytometry: optimised methods for clinical studies

Inger Anne Hagberg, Torstein Lyberg Research Forum, Ullevaal University Hospital, 0407 Oslo, Norway.
Pages 137-150 | Published online: 07 Jul 2009

  • Abrams C, Shattil SJ. Immunological detection of activated platelets in clinical disorders. Thromb Haemost 1991; 65: 467-73.
  • AuIt KA. Flow cytometric measurement of platelet function and reticulated platelets. Ann N Y Acad Sei 1993; 677: 293-308.
  • Nurden AT, Macchi L, Bihour C, Durrieu C, Besse P, Nurden P. Markers of platelet activation in coronary heart disease patients. Eur J Clin Invest 1994; 24 (Suppl 1): 42-5.
  • Rinder HM, AuIt KA. Platelet activation and its detection during the preparation of platelets for transfusion. Transfus Med Rev 1998; 12: 271-87.
  • Michelson AD. Flow cytometry: a clinical test of platelet function. Blood 1996; 87: 4925-36.
  • Schmitz G, Rothe G, Ruf A, Barlage S, Tschope D, Clemetson KJ, Goodall AH, Michelson AD, Nurden AT, Shankey TV. European Working Group on Clinical Cell Analysis: Consensus protocol for the flow cytometric characterisation of platelet function. Thromb Haemost 1998; 79: 885-96.
  • George JN, Pickett EB, Saucerman S, McEver RP, Kunicki TJ, Kieffer N, Newman PJ. Platelet surface glycoproteins. Studies on resting and activated platelets and platelet membrane microparticles in normal subjects, and observations in patients during adult respiratory distress syndrome and cardiac surgery. J Clin Invest 1986; 78: 340-8.
  • Abrams CS, Ellison N, Budzynski AZ, Shattil SJ. Direct detection of activated platelets and platelet-derived microparticles in humans. Blood 1990; 75: 128-38.
  • Dachary-Prigent J, Freyssinet JM, Pasquet JM, Carron JC, Nurden AT. Annexin V as a probe of aminophospholipid exposure and platelet membrane vesiculation: a flow cytometry study showing a role for free sulfhydryl groups. Blood 1993; 81: 2554-65.
  • ModyM, Lazarus AH, Semple JW, Freedman J. Preanalytical requirements for flow cytometric evaluation of platelet activation: choice of anticoagulant. Transfusion Medicine 1999; 9: 147-54.
  • Metzelaar MJ, Korteweg J, Sixma JJ, Nieuwenhuis HK. Comparison of platelet membrane markers for the detection of platelet activation in vitro and during platelet storage and cardiopulmonary bypass surgery. / Lab Clin Med 1993; 121: 579-87.
  • Metzelaar MJ, Sixma JJ, Nieuwenhuis HK. Detection of platelet activation using activation spesific monoclonal antibodies. Blood Cells 1990; 16: 85-96.
  • Janes SL, Wilson DJ, Chronos N, Goodall AH. Evaluation of whole blood flow cytometric detection of platelet bound fibrinogen on normal subjects and patients with activated platelets. Thromb Haemost 1993; 70: 659-66.
  • Hui KY, Jakubowski JA, Wyss VL, Angleton EL. Minimal sequence requirement of thrombin receptor agonist peptide. Biochem Biophys Res Commun 1992; 184: 790-6.
  • Warkentin TE, Powling MJ, Hardisty RM. Measurement of fibrinogen binding to platelets in whole blood by flow cytometry: a micromethod for the detection of platelet activation. Br J Haematol 1990; 76: 387-94.
  • Shattil SJ, Cunrangham M, Hoxie JA. Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood 1987; 70: 307-15.
  • Gratama JW, Kraan J, Adriaansen H, Hooibrink B, Levering W, Reinders P, Van den Beemd MW, Van der Holt B, Bolhuis RL. Reduction of interlaboratory variability in flow cytometric immunophenotyping by standardization of instrument set-up and calibration, and standard list mode data analysis. Cytometry 1997; 30: 10-22.
  • Shattil SJ, Hoxie JA, Cunningham M, Brass LF. Changes in the platelet membrane glycoprotein Ilb.IIIa complex during platelet activation. J Biol Chem 1985; 260: 11107-14.
  • Michelson AD. Platelet activation by thrombin can be directly measured in whole blood through the use of the peptide GPRP and flow cytometry: methods and clinical applications. Blood Coagul Fibrinolysis 1994; 5: 121-31.
  • Adelman B, Carlson P, Handin RI. Evaluation of platelet surface antigens by fluorescence flow cytometry. Methods Enzymol 1992; 215: 420-7.
  • AuIt KA, Rinder HM, Mitchell JG, Rinder CS, Lambrew CT, Hillman RS. Correlated measurement of platelet release and aggregation in whole blood. Cytometry 1989; 10: 448-55.
  • Ruf A, Patscheke H. Flow cytometric detection of activated platelets: comparison of determining shape change, fibrinogen binding, and P-selectin expression. Semin Thromb Hemost 1995; 21: 146-51.
  • Rinder HM, Tracey JB, Recht M, DeCastro L, Rinder CS, McHugh C, Smith BR. Differences in platelet alpha-granule release between normals and immune thrombocytopenic patients and between young and old platelets. Thromb Haemost 1998; 80: 457-62.
  • Matzdorff AC, Kuhnel G, Kemkes-Matthes B, Pralle H. Quantitative assessment of platelets, platelet microparticles, and platelet aggregates with flow cytometry. / Lab Clin tied 1998; 131: 507-17.
  • Hopwood D. Fixation and fixative. In: Bancroft JD, Stevens A, eds. Theory end Practice of Histological Techniques. London: Churchill Livingstone, 1982: 20-40.
  • Cahill MR, Macey MG, Newland AC. Fixation with formaldehyde induces expression of activation dependent platelet membrane glycoproteins, P selectin (CD62) and GP53 (CD63). Br J Haematol 1993; 84: 527-9.
  • Adelman B, Gennings C, Strony J, Hanners E. Synergistic inhibition of platelet aggregation by fibrinogen-related peptides. Ore Res 1990; 67: 941-7.
  • Holmsen H. Significance of testing platelet functions in vitro. Eur J Clin Invest 1994; 24 (Suppl 1): 3-8.
  • Bode AP, Orton SM, Frye MJ, Udis BJ. Vesiculation of platelets during in vitro aging. Blood 1991; 77: 887-95.
  • Hagberg IA, Akkok CA, Lyberg T, Kjeldsen-Kragh J. Apheresisinduced platelet activation: comparison of three types of cell separators. Transfusion 2000; 40: 182-92.
  • Knight CJ, Panesar M, Wright C, Clarke D, Butowski PS, Patel D, Patrineli A, Fox K, Goodall AH. Altered platelet function detected by flow cytometry. Effects of coronary artery disease and age. Arterioscler Thromb Vase Biol 1997; 17: 2044-53.
  • Jy W, Horstman LL, Park H, Mao WW, Valant P, Ahn YS. Platelet aggregates as markers of platelet activation: characterization of flow cytometric method suitable for clinical applications. Am J Hematol 1998; 57: 33-42.
  • Hagberg IA, Roald HE, Lyberg T. Platelet activation in flowing blood passing growing arterial thrombi. Arterioscler Thromb Vase Biol 1997; 17: 1331-6.
  • Hagberg IA, Soelvik UO, Opdahl H, Roald HE, Lyberg T. Inhalation of nitric oxide inhibits ADP-induced platelet aggregation and alpha-granule release. Platelets 1999; 10: 399-407.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.