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Original Article

Evidence that the purinergic receptor P2Y12 potentiates platelet shape change by a Rho kinase-dependent mechanism

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Pages 415-429 | Received 12 Jan 2005, Accepted 14 Feb 2005, Published online: 07 Jul 2009

References

  • Easton JD. Evidence with antiplatelet therapy and adp-receptor antagonists. Cerebrovascular Diseases 2003;16(Suppl 1):20–26.
  • Cattaneo M, Lombardi R, Zighetti ML, Gachet C, Ohlmann P, Cazenave JP, et al. Deficiency of (33P)2MeS-ADP binding sites on platelets with secretion defect, normal granule stores and normal thromboxane A2 production. Evidence that ADP potentiates platelet secretion independently of the formation of large platelet aggregates and thromboxane A2 production. Thromb Haemost 1997;77(5):986–990.
  • Eckly A, Gendrault JL, Hechler B, Cazenave JP, Gachet C. Differential involvement of the P2Y1 and P2YT receptors in the morphological changes of platelet aggregation. Thromb Haemost 2001;85(4):694–701.
  • Ralevic V, Burnstock G. Receptors for purines and pyrimidines. Pharmacol Rev 1998;50(3):413–492.
  • Leon C, Hechler B, Freund M, Eckly A, Vial C, Ohlmann P, et al. Defective platelet aggregation and increased resistance to thrombosis in purinergic P2Y(1) receptor-null mice. J Clin Invest 1999;104(12):1731–1737.
  • Hollopeter G, Jantzen HM, Vincent D, Li G, England L, Ramakrishnan V, et al. Identification of the platelet ADP receptor targeted by antithrombotic drugs. Nature 2001;409(6817):202–207.
  • Fabre JE, Nguyen M, Latour A, Keifer JA, Audoly LP, Coffman TM, et al. Decreased platelet aggregation, increased bleeding time and resistance to thromboembolism in P2Y1- deficient mice. Nature Medicine 1999;5(10):1199–1202.
  • Jin J, Daniel JL, Kunapuli SP. Molecular basis for ADP- induced platelet activation II. The P2Y1 receptor mediates ADP-induced intracellular calcium mobilization and shape change in platelets. J Biol Chem 1998;273(4):2030–2034.
  • Wilde JI, Retzer M, Siess W, Watson SP. ADP-induced platelet shape change: An investigation of the signalling pathways involved and their dependence on the method of platelet preparation. Platelets 2000;11(5):286–295.
  • Paul BZ, Daniel JL, Kunapuli SP. Platelet shape change is mediated by both calcium-dependent and–independent signaling pathways. Role of p160 Rho-associated coiled- coil-containing protein kinase in platelet shape change. J Biol Chem 1999;274(40):28293–28300.
  • Riondino S, Gazzaniga PP, Pulcinelli FM. Convulxin induces platelet shape change through myosin light chain kinase and Rho kinase. Eur J Biochem 2002;269(23):5878–5884.
  • Bauer M, Retzer M, Wilde JI, Maschberger P, Essler M, Aepfelbacher M, et al. Dichotomous regulation of myosin phosphorylation and shape change by Rho-kinase and calcium in intact human platelets. Blood 1999;94(5):1665–1672.
  • Paul BZ, Kim S, Dangelmaier C, Nagaswami C, Jin J, Hartwig JH, et al. Dynamic regulation of microtubule coils in ADP-induced platelet shape change by p160ROCK (Rho-kinase). Platelets 2003;14(3):159–169.
  • Nieswandt B, Schulte V, Zywietz A, Gratacap MP, Offermanns S. Costimulation of Gi- and G12/G13-mediated signaling pathways induces integrin alpha IIbbeta 3 activation in platelets. J Biol Chem 2002;277(42):39493–39498.
  • Jung SM, Moroi M. Platelet collagen receptor integrin alpha2beta1 activation involves differential participation of ADP-receptor subtypes P2Y1 and P2Y12 but not intracellular calcium change. Eur J Biochem 2001;268(12):3513–3522.
  • Yang J, Wu J, Jiang H, Mortensen R, Austin S, Manning DR, et al. Signaling through Gi family members in platelets. Redundancy and specificity in the regulation of adenylyl cyclase and other effectors. J Biol Chem 2002;277(48): 46035–46042.
  • Savi P, Pflieger AM, Herbert JM. cAMP is not an important messenger for ADP-induced platelet aggregation. Blood Coagul Fibrinolysis 1996;7(2):249–252.
  • Hirsch E, Bosco O, Tropel P, Laffargue M, Calvez R, Altruda F, et al. Resistance to thromboembolism in PI3Kgamma-deficient mice. FASEB J 2001;15(11): 2019–2021.
  • Kauffenstein G, Bergmeier W, Eckly A, Ohlmann P, Leon C, Cazenave JP, et al. The P2Y(12) receptor induces platelet aggregation through weak activation of the alpha(IIb)beta(3) integrin–A phosphoinositide 3-kinase-dependent mechanism. FEBS Lett 2001;505(2):281–290.
  • Kovacsovics TJ, Bachelot C, Toker A, Vlahos CJ, Duckworth B, Cantley LC, et al. Phosphoinositide 3-kinase inhibition spares actin assembly in activating platelets but reverses platelet aggregation. J Biol Chem 1995;270(19):11358–11366.
  • Trumel C, Payrastre B, Plantavid M, Hechler B, Viala C, Presek P, et al. A key role of adenosine diphosphate in the irreversible platelet aggregation induced by the PAR1- activating peptide through the late activation of phosphoinosi- tide 3-kinase. Blood 1999;94(12):4156–4165.
  • Lova P, Paganini S, Sinigaglia F, Balduini C, Torti M. A Gi-dependent pathway is required for activation of the small GTPase Rap1B in human platelets. J Biol Chem 2002;277(14):12009–12015.
  • Woulfe D, Jiang H, Mortensen R, Yang J, Brass LF. Activation of Rap1B by Gi family members in platelets. J Biol Chem 2002;277(26):23382–23390.
  • Larson MK, Chen H, Kahn ML, Taylor AM, Fabre JE, Mortensen RM, et al. Identification of P2Y12-dependent and -independent mechanisms of glycoprotein VI- mediated Rap1 activation in platelets. Blood 2003;101(4): 1409–1415.
  • Soulet C, Sauzeau V, Plantavid M, Herbert JM, Pacaud P, Payrastre B, et al. Gi-dependent and -independent mecha- nisms downstream of the P2Y12 ADP-receptor. J Thromb Haemost 2004;2(1):135–146.
  • Schoenwaelder SM, Hughan SC, Boniface K, Fernando S, Holdsworth M, Thompson PE, et al. RhoA sustains integrin alpha IIbbeta 3 adhesion contacts under high shear. J Biol Chem 2002;277(17):14738–14746. Epub 5 February 2002.
  • Jagroop IA, Burnstock G, Mikhailidis DP. Both the ADP receptors P2Y1 and P2Y12, play a role in controlling shape change in human platelets. Platelets 2003;14(1):15–20.
  • Jagroop IA, Matsagas MI, Geroulakos G, Mikhailidis DP. The effect of clopidogrel, aspirin and both antiplatelet drugs on platelet function in patients with peripheral arterial disease. Platelets 2004;15(2):117–125.
  • Daniel JL, Dangelmaier C, Jin J, Ashby B, Smith JB, Kunapuli SP. Molecular basis for ADP-induced platelet activation. I. Evidence for three distinct ADP receptors on human platelets. J Biol Chem 1998;273(4):2024–2029.
  • Mateos-Trigos G, Evans RJ, Heath MF. Effects of P2Y(1) and P2Y(12) receptor antagonists on ADP-induced shape change of equine platelets: Comparison with human platelets. Platelets 2002;13(5–6):285–292.
  • Poole AW, Watson SP. Regulation of cytosolic calcium by collagen in single human platelets. Brit J Pharmacol 1995;115(1):101–106.
  • Contreres JO, Dupuy E, Job B, Habib A, Bryckaert M, Rosa JP, et al. Effect of clopidogrel administration to healthy volunteers on platelet phosphorylation events triggered by ADP. Brit J Haematol 2003;120(4):633–642.
  • Falet H, Hoffmeister KM, Neujahr R, Hartwig JH. Normal Arp2/3 complex activation in platelets lacking WASp. Blood 2002;100(6):2113–2122.
  • Shattil SJ, Cunningham M, Hoxie JA. Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood 1987;70:307–315.
  • Riento K, Guasch RM, Garg R, Jin B, Ridley AJ. RhoE binds to ROCK I and inhibits downstream signaling. Mol Cell Biol 2003;23:4219–4229.
  • Bauer M, Maschberger P, Quek L, Briddon SJ, Dash D, Weiss M, et al. Genetic and pharmacological analyses of involvement of Src-family, Syk and Btk tyrosine kinases in platelet shape change. Src-kinases mediate integrin alphaIIb beta3 inside-out signalling during shape change. Thromb Haemost 2001;85(2):331–340.
  • Baurand A, Raboisson P, Freund M, Leon C, Cazenave JP, Bourguignon JJ, et al. Inhibition of platelet function by administration of MRS2179, a P2Y1 receptor antagonist. Eur J Pharmacol 2001;412(3):213–221.
  • Cattaneo M, Gachet C. The platelet ADP receptors. Haematologica 2001;86(4):346–348.
  • Gachet C. ADP receptors of platelets and their inhibition. Thromb Haemost 2001;86(1):222–232.
  • Kunapuli SP, Dorsam RT, Kim S, Quinton TM. Platelet purinergic receptors. Curr Opin Pharmacol 2003;3(2): 175–180.
  • Resendiz JC, Feng S, Ji G, Francis KA, Berndt MC, Kroll MH. Purinergic P2Y12 receptor blockade inhibits shear-induced platelet phosphatidylinositol 3-kinase activation. Molec Pharmacol 2003;63(3):639–645.
  • Hardy AR, Jones ML, Mundell SJ, Poole AW. Reciprocal cross-talk between P2Y1 and P2Y12 receptors at the level of calcium signaling in human platelets. Blood 2004;104(6): 1745–1752.
  • Fukata Y, Kaibuchia K, Amanob M, Kaibuchi K. Rho–Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells. Trends Pharmacol Sci 2001;22:32–39.
  • Kimura K, Ito M, Amano M, Chihara K, Fukata Y, Nakafuku M, et al. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase). Science 1996;273(5272):245–248.
  • Fox SC, Behan MW, Heptinstall S. Inhibition of ADP- induced intracellular Ca2þ responses and platelet aggregation by the P2Y12 receptor antagonists AR-C69931MX and clopidogrel is enhanced by prostaglandin E1. Cell Calcium 2004;35(1):39–46.
  • Sage SO, Yamoah EH, Heemskerk JW. The roles of P(2X1)and P(2T AC)receptors in ADP-evoked calcium signalling in human platelets. Cell Calcium 2000;28(2): 119–126.
  • Missy K, Plantavid M, Pacaud P, Viala C, Chap H, Payrastre B. Rho-kinase is involved in the sustained phosphorylation of myosin and the irreversible platelet aggregation induced by PAR1 activating peptide. Thromb Haemost 2001;85:514–520.
  • Maayani S, Tagliente TM, Schwarz T, Martinelli G, Martinez R, Shore-Lesserson L. The balance of concurrent aggregation and deaggregation processes in platelets is linked to differential occupancy of ADP receptor subtypes. Platelets 2001;12(2):83–93.
  • Foster CJ, Prosser DM, Agans JM, Zhai Y, Smith MD, Lachowicz JE, et al. Molecular identification and character- ization of the platelet ADP receptor targeted by thienopyridine antithrombotic drugs. J Clin Invest 2001;107(12):1591–1598.
  • Begum N, Sandu OA, Duddy N. Negative regulation of rho signaling by insulin and its impact on actin cytoskeleton organization in vascular smooth muscle cells: Role of nitric oxide and cyclic guanosine monophosphate signaling pathways. Diabetes 2002;51:2256–2263.
  • Klages B, Brandt U, Simon MI, Schultz G, Offermanns S. Activation of G12/G13 results in shape change and Rho/Rho- kinase-mediated myosin light chain phosphorylation in mouse platelets. J Cell Biol 1999;144(4):745–754.
  • Essler M, Staddon JM, Weber PC, Aepfelbacher M. Cyclic AMP blocks bacterial lipopolysaccharide-induced myosin light chain phosphorylation in endothelial cells through inhibition of Rho/Rho kinase signaling. J Immunol 2000;164:6543–6549.
  • Sawada N, Itoh H, Yamashita J, Doi K, Inoue M, Masatsugu K, et al. cGMP-dependent protein kinase phos- phorylates and inactivates RhoA. Biochem Biophys Res Commun 2001;280:798–805.
  • Ishii-Watabe A, Uchida E, Mizuguchi H, Hayakawa T. Involvement of a calcium-independent pathway in plasmin- induced platelet shape change. Life Sci 2001;69:945–960.
  • Amano M, Ito M, Kimura K, Fukata Y, Chihara K, Nakano T, et al. Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase). J Biol Chem 1996;271(34):20246–20249.
  • Ingall AH, Dixon J, Bailey A, Coombs ME, Cox D, McInally JI, et al. Antagonists of the platelet P2T receptor: A novel approach to antithrombotic therapy. J Med Chem 1999;42(2):213–220.
  • Crosby D, Poole AW. Interaction of Bruton’s tyrosine kinase and protein kinase Ctheta in platelets. Cross-talk between tyrosine and serine/threonine kinases. J Biol Chem 2002;277(12):9958–9965.
  • Hardy AR, Conley PB, Luo J, Benovic JL, Poole AW, Mundell SJ. P2Y1 and P2Y12 receptors for ADP desensitize by distinct kinase-dependent mechanisms. Blood 2005; 105(9):3552–3560.
  • Jagroop IA, Clatworthy I, Lewin J, Mikhailidis DP. Shape change in human platelets: Measurement with a channelyzer and visualisation by electron microscopy. Platelets 2000;11(1):28–32.
  • Amano T, Tanabe K, Eto T, Narumiya S, Mizuno K. LIM-kinase 2 induces formation of stress fibres, focal adhesions and membrane blebs, dependent on its activation by Rho-associated kinase-catalysed phosphorylation at threonine-505. Biochem J 2001;354(Pt 1):149–159.
  • Toshima J, Toshima JY, Amano T, Yang N, Narumiya S, Mizuno K. Cofilin phosphorylation by protein kinase testicular protein kinase 1 and its role in integrin-mediated actin reorganization and focal adhesion formation. Mol Biol Cell 2001;12(4):1131–1145.
  • Lou Z, Billadeau DD, Savoy DN, Schoon RA, Leibson PJ. A role for a RhoA/ROCK/LIM-kinase pathway in the regulation of cytotoxic lymphocytes. J Immunol 2001;167(10):5749–5757.

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