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International Journal of Radiation Biology Volume 77, 2001 - Issue 3
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Research Article

A higher micronucleus yield in B- versus T-cells after low-dose γ-irradiation is not linked with defective Ku86 protein

A. Vral Department of Anatomy, Embryology, Histology and Medical Physics, Section Histology, Ghent University, L. Pasteurlaan 2, B-9000 Gent, Belgium
,
H. Thierens Medical Physics, Ghent University, L. Pasteurlaan 2, B-9000 Gent, Belgium
,
P. Bryant School of Biology, University of St Andrews, St Andrews KY 16 9TS, UK
&
L. De Ridder Department of Anatomy, Embryology, Histology and Medical Physics, Section Histology, Ghent University, L. Pasteurlaan 2, B-9000 Gent, Belgium
Pages 329-339 | Published online: 03 Jul 2009

  • CASELLAS, R., NUSSENZWEIG, A., WUERFFEL, R., PELANDA, R., REICHLIN, A., SUH, H., QIN, X. F., BESMER, E., RENTER, A., RAJEWSKY, K. and NUSSENZWEIG, M. C., 1998, Ku80 is required for immunoglobulin isotype switching. European Molecular Biology Organization Journal, 17, 2404-2411.
  • CORNELISSEN, M., VRAL, A., THIERENS, H. and DE RIDDER, L., 1999, The effect of caspase-inhibitors on radiation induced apoptosis in human peripheral blood lymphocytes: an electron microscopic approach. Apoptosis, 4, 449-454.
  • FENECH, M., 1993, The cytokinesis-block micronucleus assay: a detailed description of the method and its application to genotoxicity studies in human populations. Mutation Research, 285, 35-44.
  • FINNIE, N., GOTTLIEB, T. M., BLUNT, T., JEGGO, P. A. and JACKSON, S. P., 1995, DNA-dependent protein kinase activity is absent in x?s-6 cells: implications for site-specific recombination and DNA double-strand break repair. Proceedings of the National Academy of Sciences, USA, 92, 320-324.
  • HOLMEN, A., KARLSSON, A., BRATT, I. and HOGSTED, B., 1994, Micronuclei and mitotic index in B, T4 and T8 cells treated by mitomycin C and ?-irradiation. Mutation Research, 309, 93-99.
  • HÖGSTED, B., KARLSSON, A., BRATT, I. and HOLMEN, A., 1993, Micronucleus induction in human B and T lymphocytes separated by an immunomagnetic technique. Hereditas, 119, 99-103.
  • JEGGO, P. A., 1998, Identification of genes involved in repair of DNA double-strand breaks in mammalian cells. Radiation Research, 150 (suppl.), S80-91.
  • JOINER, M. C., 1994, Induced radioresistance: an overview and historical perspective. International Journal of Radiation Biology, 65, 79-84.
  • KANAAR, R., HOEIJMAKERS, J. H. J. and VANGENT, D. C., 1998, Molecular mechanisms of DNA double-strand break repair. Trends in Cell Biology, 8, 483-489.
  • KEMP, L. M., SEDGEWICK, S. G. and JEGGO, P. A., 1984, X-ray sensitive mutants of Chinese hamster ovary cells defective in double-strand break rejoining. Mutation Research, 132, 189-196.
  • LAMBIN, P., MALAISE, E. P. and JOINER, M. C., 1996, Might intrinsic radioresistance of human tumour cells be induced by radiation? International Journal of Radiation Biology, 69, 279-290.
  • LAMBIN, P. MARPLES, B. FERTIL, B. MALAISE, E. P. and JOINER, M. C., 1993, Hypersensitivity of a human tumour cell line to very low radiation doses. International Journal of Radiation Biology, 63, 639-650.
  • LOUAGIE, H., PHILIPPE, J., VRAL, A., CORNELISSEN, M., THIERENS, H. and DE RIDDER, L., 1998, Induction of micronuclei and apoptosis in natural killer cells compared with T lymphocytes after ?-irradiation. International Journal of Radiation Biology, 73, 179-185.
  • LOUAGIE, H., SCHOTTE, P., VRAL, A., CORNELISSEN, M., THIERENS, H., BEYAERT, R., DE RIDDER, L. and PHILIPPÉ, J., 1999, Apoptosis induced by ? irradiation in peripheral blood mononuclear cells is not mediated by cytochromeC release and only partially involves caspase-3 like proteases. Cell Biology International, 23, 611-617.
  • MARPLES, B. and JOINER, M. C., 2000, Modification of survival by DNA repair modifiers: a probable explanation for the phenomenon of increased radioresistance. International Journal of Radiation Biology, 76, 305-312.
  • MARPLES, B., LAM, G. K. Y., ZHOU, H. and SKOV, K. A., 1994, The response of Chinese hamster V79-379A cells exposed to negative Pi-mesons: evidence that increased radioresistance is dependent on linear energy transfer. Radiation Research, 138, S81-84.
  • MARPLES, B., LAMBIN, P., SKOV, K. A. and JOINER, M. C., 1997, Low dose hyper-sensitivity and increased radioresistance in mammalian cells. International Journal of Radiation Biology, 71, 721-735.
  • MORIO, T., HANISSIAN, S. H., BACHARIER, L. B., TERAOKA, H., NONOYAMA, S., SEKI, M., KONDO, J., NAKANO, H., LEE, S., GEHA, R. S. and YATA, J., 1999, Ku in the cytoplasm associates with CD40 in human B-cells and translocates into the nucleus following incubation with IL-4 and antiCD40mAb. Immunity, 11, 339-348.
  • MULLER, C., DUSSEAU, C., CALSOU, P. and SALLES, B., 1998, Human normal peripheral blood lymphocytes are deficient in DNA-dependent protein kinase activity due to the expression of a variant form of the Ku86 protein. Oncogme, 16, 1553-1560.
  • PETERSON, S. R., STACKHOUSE, M., WALTMAN, M. J., CHEN, F., SATO, K. and CHEN, D. J., 1997, Characterization of two DNA double-stranded break repair-deficient cell lines that express inactive DNA-dependent protein kinase catalytic subunits. Journal of Biological Chemistry, 272, 1022710231.
  • PHILIPPÉ, J., LOUAGIE, H., THIERENS, H., VRAL, A., CORNELISSEN, M. and DE RIDDER, L., 1997, Quantification of apoptosis in lymphocyte subsets and effect of apoptosis on apparent expression of membrane antigens. Cytometry, 29, 242-249.
  • ROBSON, T., JOINER, M. C., WILSON, G. D., MCCULLOUGH, W., PRICE, M. E., LOGAN, I., JONES, H., MCKEOWN, S. R. and HIRST, D. G., 1999, A novel human stress responserelated gene with a potential role in induced radioresistance. Radiation Research, 152, 451-461.
  • SCHWARTZ, J. L. and GAULDEN, M. E., 1980, The relative contribution of B and T lymphocytes in the human peripheral blood mutagen test system as described by cell survival, mitogenic stimulation, and induction of chromosome aberrations by irradiation. Environmental Mutagenesis, 2, 473-485.
  • SCHWARTZ, J. L., DARR, J. C. and GAULDEN, M. E., 1983, Survival and PHA stimulation of gamma-irradiated human peripheral blood T-lymphocyte subpopulations. Mutation Research, 107, 413-425.
  • SEKI, H., KANEGANE, H., IWAI, K., KONNO, A., OHTA, K., YACHIE, A., TANIGUCHI, N. and MIYAWAKI, T., 1994, ionising radiation induces apoptotic cell death in human TcRgamma/delta + T and natural killer cells without detectable P53 protein. European Journal of Immunology, 24, 2914-2917.
  • SIEBER, G., ZIERACH, P., HERRMANN, F., BRUST, V. J. and RÜHL, H., 1985, Impaired B lymphocyte reactivity in patients after radiotherapy. International Journal of Radiation Oncology, Biology and Physics, 11, 777-782.
  • STEWART, C. C., STEVENSON, A. P. and HABBERSETT, R. G., 1988, The effect of low-dose irradiation on unstimulated and PHA-stimulated human lymphocyte subsets. International Journal of Radiation Biology, 53, 77-87.
  • VAGANAY-JUERY, S., MULLER, C., MARANGONI, E., ABDULKARIM, B., DEUTSCH, E., LAMBIN, P., ESCHWEGE, F., SALLES, B., JOINER, M. and BOURHIS, J., 2000, Decreased DNA-PK activity in human cancer cells exhibiting hypersensitivity to low dose irradiation. British Journal of Cancer, 83, 514-518.
  • VRAL, A., LOUAGIE, H., THIERENS, H., PHILIPPÉ, J., CORNELISSEN, M. and DE RIDDER, L., 1998, Micronucleus frequencies in cytokinesis-blocked human B lymphocytes after low dose ?-irradiation. International Journal of Radiation Biology, 73, 549-555.
  • VRAL, A., VERHAEGEN, F., THIERENS, H. and DE RIDDER, L., 1994a, Micronuclei induced by fast neutrons versus 60Co ?-rays in human peripheral blood lymphocytes. International Journal of Radiation Biology, 65, 321-328.
  • VRAL, A., VERHAEGEN, F., THIERENS, H. and DE RIDDER, L., 1994b, The in vitro cyotkinesis-block micronucleus assay: a detailed description of an improved slide preparation technique for the automated detection of micronuclei in human lymphocytes. Mutagerusis, 9, 439-443.
  • WUERFFEL, R. A., THOMPSON, R. J. and KENTER, A. L., 1997, IgS?3 DNA-specific double-strand breaks are induced in mitogen-activated B-cells and are implicated in switch recombination. Journal of Immunology, 159, 4139-4144.
  • WÜTTKE, K., STREFFER, C. and MÜLLER, W.-U., 1993, Radiation induced micronuclei in subpopulations of human lymphocytes. Mutation Research, 286, 181-188.

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