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Research Article

Effects of NF-κB1 (p50) targeted gene disruption on ionizing radiation-induced NF-κB activation and TNFα, IL-1α, IL-1β and IL-6 mRNA expression in vivo

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Pages 763-772 | Published online: 03 Jul 2009

  • decreased susceptibility of p50Õ/Õ mice to various Attar, R. M., Caamano, J., Carrasco, D., Iotsova, V.,
  • in? ammatory diseases (Yang 1998, Hilliard 1999, Ishikawa, H., Ryseck, R. P., Weih, F. and Bravo, R.,
  • Campbell 2000). However, certain levels of residual 1997, Genetic approaches to study Rel/NF-kappa B/I
  • inducibility for TNFa, IL-1a, IL-1b and IL-6 mRNA kappa B function in mice. Seminars in Cancer Biology,
  • expression by IR remained to be seen in p50Õ/Õ 8, 93-101. Baeuerle, P. A. and Henkel, T., 1994, Function and activation
  • mice. The lack of complete inhibition of IR-induced of NF-kappa B in the immune system. Annual Review of
  • cytokine mRNA expression in p50Õ/Õ mice suggests Immunology, 12, 141-179.
  • that other IR-responsive transcription factors may Baker, W. H., Limanni, A., Chang, C. M., Jackson, W. E.,
  • also contribute to IR-induced production of these Seemann, R. and Patchen, M. L., 1995, Comparison of
  • cytokines, which will be further investigated. interleukin-1 alpha gene expression and protein levels in the murine spleen after lethal and sublethal total-bodyVariable increases in TNFa, IL-1a, IL-1b and IL-6 irradiation. Radiation Research, 143, 320-326.
  • mRNA expression have also been found in the brain Baldwin, A. S. J., 1996, The NF-kappa B and I kappa B
  • and lungs in rats or mice exposed to IR (Rubin et al. proteins: new discoveries and insights. Annual Review of
  • 1995, Johnston et al. 1996, Chiang et al. 1997, Hong Immunology, 14, 649-683.
  • etal.1999, Raju et al. 1999). In rat brain, the
  • Beg AA, S. W. B. R. G., 1995, Embryonic lethality and liver degeneration in mice lacking the RelA component ofincreased expression of TNFa, IL-1b and IL-6 NF-kappa B. Nature, 376, 167-170.mRNA paralleled NF-kB activation (Raju et al. 1999).
  • Brach, M. A., Gruss, H. J., Kaisho, T., Asano, Y., Hirano, T.The relationship between NF-kB activation and and Herrmann, F., 1993, Ionizing radiation induces
  • increased expression of these cytokines mRNA in the expression of interleukin 6 by human ? broblasts involving
  • lungs is not known. Thus, it will be of our interest activation of nuclear factor-kappa B. Journal of Biological Chemistry, 268, 8466-8472.
  • to further examine the role of NF-kB activation in Brach, M. A., Hass, R., Sherman, M. L., Gunji, H.,

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