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International Journal of Radiation Biology Volume 79, 2003 - Issue 3
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Research Article

Differential effectiveness of solar UVB subcomponents in causing cell death, oncogenic transformation and micronucleus induction in human hybrid cells

D. Bettega Dipartimento di Fisica, Università degli Studi di Milano and INFN, via Celoria 16, I-20133 Milan, Italy
,
P. Calzolari Dipartimento di Fisica, Università degli Studi di Milano and INFN, via Celoria 16, I-20133 Milan, Italy
,
L. Doneda Dipartimento di Biologia e Genetica, Università degli Studi di Milano, via Viotti 3/5, I-20133 Milan, Italy
,
F. Belloni Laboratori Nazionali di Frascati, Frascati, I-00044 Rome, Italy
,
L. Tallone Dipartimento di Fisica, Università degli Studi di Milano and INFN, via Celoria 16, I-20133 Milan, Italy
&
J. L. Redpath Department of Radiation Oncology, University of California. Irvine, CA 92697, USA
Pages 211-216 | Published online: 03 Jul 2009

  • BETTEGA, D., CALZOLARI, P., BELLONI, F., Di LENA, F., GENCHI, S., LUPI, M., MASSARIELLO. P., ORSINI, S., TALLONE, L., TOMASONI, D., UBEZIO, P. and REDPATH, J. L., 2001, Solar UV radiation: differential effectiveness of UVB subcomponents in causing cell death, micronucleus induction and delayed expression of heritable damage in human hybrid cells. International Journal of Radiation Biology, 77, 963-970.
  • BETTEGA, D., CALZOLARI, P., DOGLIA, S. M., DULIO, B., TALLONE, L. and VILLA, A., M., 1998, Technical Report: Cell thickness measurements by confocal fluorescence microscopy on C3H10T1/2 and V79 cells. International Journal of Radiation Biology, 74, 397-403.
  • BETTEGA, D., CALZOLARI, P., PIAZZOLLA, A., TALLONE, L. and REDPATH, J. L., 1997, Alpha-particle-induced neoplastic transformation in synchronized hybrid cells of HeLa and human skin fibroblasts. International Journal of Radiation Biology, 72, 523-529.
  • CHAN, G. L. and LITTLE, J. B., 1976, Induction of oncogenic transformation in vitro by ultraviolet. Nature, 264, 442-444.
  • DE GRUIJL, F. R., 1999, Skin cancer and solar UV radiation. European Journal of Cancer, 35, 2003-2009.
  • DI PAOLO, J. A. and DONOVAN, P. J., 1976, In vitro morphologic transformation of Syrian hamster cells by irradiation is enhanced by X-irradiation and unaffected by chemical carcinogens. International Journal of Radiation Biology, 30, 41-53.
  • DIEPGEN, T. L. and MAHLER, V., 2002, The epidemiology of skin cancer. British Journal of Dermatology, 146, 1-6.
  • DONEDA, L., CASTORINA, P., TEDESCHI, A., INTROPIDO, L, MORRA, E., MONTILLO, M. and LARIZZA, L., 2001, Multicolor FISH in chronic lymphocytic leukemia. An interphase study of patients with early-onset disease. Cancer Genetics and Cytogenetics, 125, 63-69.
  • DONIGER, J., JACOBSON, E. D., KRELL, K. and Di PAOLO, J. A., 1981, Ultraviolet light action spectra for neoplastic transformation and lethality of Syrian hamster embryo cells correlate with spectrum for pyrimidine dimer formation in cellular DNA. Proceedings of the National Academy of Sciences, USA, 78, 2378-2382.
  • EMRI, G., WENCZL, E., VAN ERP, P., JANS, J., ROZA, L., HORKAY, I. and SCHOTHORST, A. A., 2000, Low doses of UVB or UVA induce chromosomal aberrations in cultured human skin cells. Journal of Investigative Dermatology, 115, 435-440.
  • FENECH, M., 1997, The advantages and disadvantages of the cytokinesis-block micronucleus method. Mutation Research, 392, 11-18.
  • ICNIRP, 1996, Guidelines on UV radiation exposure limits. Health Physics, 71, 978.
  • INIRC/IRPA, 1985, Guidelines on limits of exposure to ultraviolet radiation of wavelengths between 180 run and 400 run (incoherent optical radiation). Health Physics, 49, 331-340.
  • INIRC/IRPA, 1989, Proposed change to the IRPA 1985 guidelines on limits of exposure to ultraviolet radiation. Health Physics, 56, 971-972.
  • KEULERS, R. A. C., DE ROON, A. R, DE ROODE, S. and TATES, A. D., 1998, The induction and analysis of micronuclei and cell killing by ultraviolet-B radiation in human peripheral blood lymphocytes. Photochemistry and Photobiology, 67, 426-432.
  • KEULERS, R. A. C., VAN TEYLINGEN, C. M. M. and TATES, A. D., 2000, Effects of deoxyribonucleosides and cellstimulation on frequencies of ultraviolet-B-induced micronuclei in human peripheral blood lymphocytes. Mutation Research, 459, 115-122.
  • LITTLE, J. B., 1998, Radiation-induced genomic instability. International Journal of Radiation Biology, 74, 663-671.
  • MENDONCA, M. S., ANTONIONO, R.J. and REDPATH, J. L., 1993, Delayed heritable damage and epigenetics in radiationinduced neoplastic transformation of human hybrid cells. Radiation Research, 134, 209-216.
  • MENDONCA, M. S., ANTONIONO, R. J., SUN, C. and REDPATH, J. L., 1992, A simplified and rapid staining method for the HeLa x Skin fibroblast human hybrid cell neoplastic transformation assay. Radiation Research, 131, 345-350.
  • MENDONCA, M. S., HOWARD, K., FASCHING, C. L., FARRINGTON, D. L., DESMOND, L. A., STANBRIDGE, E. J. and REDPATH, J. L., 1998b, Loss of suppressor loci on chromosomes 11 and 14 may be required for radiation-induced neoplastic transformation on HeLa x fibroblast human cell hybrids. Radiation Research, 149, 246-255.
  • MENDONCA, M. S., TEMPLES, T. M., FARRINGTON, D. L. and BLOCK, C., 1998a, Evidence for a role of delayed death and genomic instability in radiation-induced neoplastic transformation of human hybrid cells. International Journal of Radiation Biology, 74, 755-764.
  • MILLER, B. M., WERNER, T., WEIER, H. U. and NUSSE, M., 1992, Analysis of radiation-induced micronuclei by fluorescence in situ hybridization (FISH) simultaneously using telomeric and centromeric DNA probes. Radiation Research, 131, 177-185.
  • MOTHERSILL, C., CREAN, M., LYONS, M., MCSWEENEY, J., MOONEY, R., O'REILLY, J. and SEYMOUR, C. B., 1998, Expression of delayed toxicity and lethal mutations in the progeny of human cells surviving exposure to radiation and other environmental mutagens. International Journal of Radiation Biology, 74, 673-680.
  • O'REILLY, J. P. and MOTHERSILL, C., 1997, Comparative effects of UVA and UVB on clonogenic survival and delayed cell death in skin cell lines from humans and fish. International Journal of Radiation Biology, 72, 111-119.
  • PAPADOPOULO, D., MUEL, B. and LATARJET, R., 1983, Relative efficiencies of three ultraviolet radiation wavelengths for cell killing and transformation in mouse cells in vitro. International Journal of Radiation Biology, 44, 241-249.
  • RAMIREZ, M. J., SURRALLES, J., PUERTO, S., CREUS, A. and MARCOS, R., 1999, Low persistence of radiation-induced centromere positive and negative micronuclei in cultured human cells. Mutation Research, 440, 163-169.
  • REDPATH, J. L. and SUN, C., 1989, UVC-induced expression of a tumor-associated antigen in human cell hybrids (HeLa x skin fibroblasts): repair of potentially lethal and potentially transforming damage. Cardaogenesis, 10, 723-726.
  • REDPATH, J. L., SUN, C., COLMAN, M. and STANBRIDGE, E. J., 1987, Neoplastic transformation of human hybrid cells by radiation: a quantitative assay. Radiation Research, 110, 468-472.
  • SEYMOUR, C. B., MOTHERSILL, c. and ALPER, T., 1986, High yields of lethal mutations in somatic mammalian cells that survive ionizing radiation. International Journal of Radiation Biology, 50, 167-179.
  • STAMATO, T. D., RICHARDSON, E. and PEREZ, M. L., 1995, UVlight induces delayed mutations in Chinese hamster cells. Mutation Research, 328, 175-181.
  • STANBRIDGE, E. J., DER, C. J., DOERSEN, C. J., NISHIMI, R. Y., PEEHL, D. M., WEINSTEIN, B. E. and WILKINSON, J. E., 1982, Human cell hybrids analysis of transformation and tumorigenicity. Science, 215, 252-259.
  • SUN, C., REDPATH, J. L., COLMAN, M. and STANBRIDGE, E. J., 1988, Further studies on the radiation-induced expression of a tumor-specific antigen in human cell hybrids. Radiation Research, 114, 84-93.
  • SUZUKI, F., HAN, A., LANKAS, G. R., UTSUMI, H. and ELKIND, M. M., 1981, Spectral dependencies of killing, mutation and transformation in mammalian cells and their relevance to hazards caused by solar ultraviolet radiation. Cancer Research, 41, 4916-4924.
  • WELLER, E. M., HAIN, J., JUNG, T., KINDER, R, KOFFERLEIN, M., BURKART, W. and NUSSE, M., 1996, UV-B induced cell cycle perturbations, micronucleus induction and modulation by caffeine in human keratinocytes. International Journal of Radiation Biology, 69, 371-384.
  • YANG, T. C., MEI, M., GEORGE, K. A. and CRAISE, L. M., 1996, Oncogenic and mutagenic effects of UV in mammalian cells. Advances in Space Research, 18, (12)17-26.

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