Lovastatin causes sensitization of HeLa cells to ionizing radiation‐induced apoptosis by the abrogation of G2 blockage

References

• ADAMSON, P., MARSHALL, C. J., HALL, A. and TrLBRoox, P. A., 1992, Post-translational modifications of p21rho proteins. Journal of Biological Chemistry, 267, 20033–20038.
   PubMed Web of Science ®Google Scholar
• ADER, I., TOULAS, C., DALENC, F., DELMAS, C., BONNET, J., Cori-JONATHAN, E. E. and FAVRE, G., 2002, RhoB controls the 24 kDa FGF-2-induced raclioresistance in HeLa cells by preventing post-mitotic cell death. Oncogene, 21, 5998–6006.
   Google Scholar
• BAO, S., TIBBETT'S, R. S., BRUMBAUGH, K. M., FANG, Y., RICHARDSON, D. A., Au, A., CHEN, S. M., ABRAHAM, R. T. and WANG XIAO, F., 2001, ATR/ATM-mediated phosphorylation of human Rad 1 7 is required for genotoxic stress responses. Nature, 411, 969–974.
   Google Scholar
• BARLOW, C., HIROTSUNE, S., PAYLOR, R., LIYANAGE, M., ECKHAUS, M., COLLINS, F., SHILOH, Y., CRAWLEY, J. N., RIED, T., TAGLE, D. and WYNSHAW, B. A., 1996, Atm-deficient mice: a paradigm of ataxia telangiectasia. Cell, 86, 159–171.
   PubMed Web of Science ®Google Scholar
• BERNHARD, E. J., MCKENNA, W. G., HAMILTON, A. D., SEBTI, S. M., QIAN, Y., Wu, J. M. and MUSCHEL, R. J., 1998, Inhibiting Ras prenylation increases the radiosensitivity of human tumor cell lines with activating mutations of ras oncogenes. Cancer Research, 58, 1754–1761.
   PubMed Web of Science ®Google Scholar
• BERNHARD, E. J., STANBRIDGE, E. J., GUPTA, S., GUPTA, A. K., SOTO, D., BAKANAUSKAS, V. J., CERNIGLIA, G. J., MUSCHEL, R. J. and McKENNA, W. G., 2000, Direct evidence for the contribution of activated N-ras and K-ras oncogenes to increased intrinsic radiation resistance in human tumor cell lines. Cancer Research, 60, 6597–6600.
   PubMed Web of Science ®Google Scholar
• CANMAN, C. E. and KmTAN, M. B., 1996, Three paths to stress relief. Nature, 384, 213–214.
   PubMed Web of Science ®Google Scholar
• CANMAN, C. E., LIM DAE, S., CIMPIUCH, K. A., TAYA, Y., TAMAI, K., SAKAGUCHE, K., APPELLA, E., KASTAN, M. B. and SrucTANo, J. D., 1998, Activation of the ATM kinase by ionizing radiation and phosphorylation of p53. Science, 281, 1677–1679.
   PubMed Web of Science ®Google Scholar
• CASPARI, T., 2000, Checkpoints: how to activate p53. Current Biology, 10, R315—R317.
   Web of Science ®Google Scholar
• Clio', J. W. and JUNG, S. E., 1999, Lovastatin-induced proliferation inhibition and apoptosis in C6 glial cells. Journal of Pharmacology and Experimental Therapeutics, 289, 572–579.
   Google Scholar
• CLIBY, W. A., ROBERTS, C. J., CIMPRICH, K. A., STRINGER, C. M., LAMB, J. R., SCHREIBER, S. L. and FRIEND, S. H., 1998, Overexpression of a kinase-inactive ATR protein causes sensitivity to DNA-damaging agents and defects in cell cycle checkpoints. EMBO Journal, 17, 159–169.
   PubMed Web of Science ®Google Scholar
• COHEN, J. E., TOULASS, C., MONTEIL, S., COUDERC, B., MARET, A., BARD, J. J., PRATS, H., DAry-ScHvErrzER, N. and FAVRE, G., 1997, Radioresistance induced by the high molecular form of basic fibroblast growth factor is associated with an increased G-2 delay and a hyperphosphorylation of p43-Cdc2 in HeLa cells. Cancer Research, 57, 1364–1370.
   Google Scholar
• COHENJONATHAN, E., TOULAS, C., ADER, I., MONTEIL, S., ALLAL, C., BONNET, J., HAMILTON, A. D., SEBTI, S. M., DALY-ScHvErrzER, N. and FAVRE, G., 1999, The farnesyl-transferase inhibitor FTI-277 suppresses the 24-kDa FGF2-induced raclioresistance in HeLa cells expressing wild-type RAS. Radiation Research, 152, 404–411.
   Google Scholar
• COOPER, S., 2002, Reappraisal of Gl-phase arrest and synchronization by lovastatin. Cell Biology International, 26, 715–727.
   PubMed Web of Science ®Google Scholar
• Cox, A. D. and DER, C. J., 1992, Protein prenylation: more than just glue? Current Opinions in Cell Biology, 4, 1008–1016.
   PubMedGoogle Scholar
• DELMAS, C., HELIEZ, C., COHEN-JONATHAN, E., END, D., BONNET, J., FAVRE, G. and TOULAS, C., 2002, Farnesyltransferase inhibitor, R115777, reverses the resistance of human glioma cell lines to ionizing radiation. International Journal of Cancer, 100, 43–48.
   Google Scholar
• DENT, P., JARvis, W. D., BIRRER, M. J., FISHER, P. B., SCHMIDT-ULLRICH, R. K. and GRANT, S., 1998, The roles of signalling by the p42/p44 mitogen-activated protein (MAP) kinase pathway; a potential route to radio- and chemo-sensitization of tumor cells resulting in the induction of apoptosis and loss of clonogenicity. Leukemia, 12, 1843–1850.
   Google Scholar
• DEVARY, Y., ROSETTE, C., DIDONATO, J. A. and KAREN, M., 1993, NFkappaB activation by ultraviolet light not dependent on a nuclear signal. Science, 261, 1442–1445.
   PubMed Web of Science ®Google Scholar
• DarrrRourAxos, J., NOHYNEK, D., BACKWAY, K. L., HEDLEY, D. W., YEGER, H., FREEDMAN, M. H., MINDEN, M. D. and PENN, L. Z., 1999, Increased sensitivity of acute myeloid leukemias to lovastatin-induced apoptosis: a potential therapeutic approach. Blood, 93, 1308–1318.
   PubMed Web of Science ®Google Scholar
• DUNKERN, T. R., FRuz, G. and KAINTA, B., 2001, Ultraviolet light-induced DNA damage triggers apoptosis in nucleotide excision repair-deficient cells via Bc1-2 decline and caspase-3/-8 activation. Oncogene, 20, 6026–6038.
   PubMed Web of Science ®Google Scholar
• DUROCHER, D. and JACKSON, S. P., 2001, DNA-PK, ATM and ATR as sensors of DNA damage: variations on a theme? Current Opinions in Cell Biology, 13, 225–231.
   PubMed Web of Science ®Google Scholar
• FOKSTUEN, T., RABO, Y. B., ZHOU, J. N., KARLSON, J., PLATZ, A., SHOSHAN, M. C., HANSSON, J. and LINDER, S., 1997, The Ras farnesylation inhibitor BZA-5B increases the resistance to cisplatin in a human melanoma cell line. Anticancer Research, 17, 2347–2352.
   PubMed Web of Science ®Google Scholar
• FRIEDBERG, E. C., WALLNER, G. C. and SIEDE, W., 1995, DM Repair and Mutagenesis (Washington, DC: ASM Press).
   Google Scholar
• FRITZ, G. and KAINA, B., 2000, Ras-related GTPase RhoB forces alkylation-induced apoptotic cell death. Biochemical and Biophysical Research Communications, 268, 784–789.
   PubMed Web of Science ®Google Scholar
• GLOMSET, J. A., GELB, M. H. and FARNSWORTH, C. C., 1990, Prenyl proteins in eukaryotic cells: a new type of membrane anchor. Trends in Biochemical Science, 15, 139–142.
   PubMed Web of Science ®Google Scholar
• GNAD, R., AKTORIES, K., KAINA, B. and FRuz, G., 2000, Inhibition of protein isoprenylation impairs Rho-regulated early cellular response to genotoxic stress. Molecular Pharmacology, 58, 1389–1397.
   PubMed Web of Science ®Google Scholar
• GNAD, R., KAINA, B. and FRuz, G., 2001, Rho GTPases are involved in the regulation of NF-kB by genotoxic stress. Experimental Cell Research, 264, 244–249.
   PubMed Web of Science ®Google Scholar
• GuvrA, A. K., BAKANAUSKAS, V. J., CERNIGLIA, G. J., CHENG, Y., BERNHARD, E. J., MUSCHEL, R. J. and MCKENNA, W. G., 2001a, The Ras radiation resistance pathway. Cancer Research, 61, 4278–4282.
   PubMed Web of Science ®Google Scholar
• GuvrA, A. K., BAKANAUSKAS, V. J., MCKENNA, W. G., BERNHARD, E. J. and MUSCHEL, R. J., 2001b, Ras regulation of radioresistance in cell culture. Methods in Enzymology, 333, 284–290.
   PubMed Web of Science ®Google Scholar
• HUANG, C., MA WET, Y., DING, M., BOWDEN, G. T. and DONG, Z., 1997, Direct evidence for an important role of sphingomyelinase in ultraviolet-induced activation of c-Jun N-terminal kinase. Journal of Biological Chemistry, 272, 27753–27757.
   PubMed Web of Science ®Google Scholar
• KAINA, B., HAAS, S. and KAPPES, H., 1997, A general role for c-Fos in cellular protection against DNA-damaging carcinogens and cytostatic drugs. Cancer Research, 57, 2721–2731.
   PubMed Web of Science ®Google Scholar
• KROEMER, G., 1997, The proto-oncogene Bc1-2 and its role in regulating apoptosis. Nature Medicine, 3, 614–620.
   PubMed Web of Science ®Google Scholar
• LAKIN, N. D., HANN, B. C. and JACKSON, S. P., 1999, The ataxia-telangiectasia related protein ATR mediates DNA-dependent phosphorylation of p53. Oncogene, 18, 3989–3995.
   PubMed Web of Science ®Google Scholar
• LAKIN, N. D. and JACKSON, S. P., 1999, Regulation of p.53 in response to DNA damage. Oncogene, 18, 7644–7655.
   PubMed Web of Science ®Google Scholar
• LEE, J. M. and BERNSTEIN, A., 1993, p53 mutations increase resistance to ionizing radiation. Proceedings of the National Academy of Sciences, USA, 90, 5742–5746.
   PubMed Web of Science ®Google Scholar
• Li, N. and KARIN, M., 1998, Ionizing radiation and short wavelength UV activate NF-kappaB through two distinct mechanisms. Proceedings of the National Academy of Sciences, USA, 95, 13012–13017.
   PubMed Web of Science ®Google Scholar
• Liu, A.-X., CERNIGLIA, G. J., BERNHARD, E. J. and PRENDERGAST, G. C., 2001, RhoB is required to mediate apoptosis in neoplastically transformed cells after DNA damage. Proceedings of the National Academy of Sciences, USA, 98, 6192–6197.
   Google Scholar
• MACLEOD, K. F., SHERRY, N., HANNON, G., BEACH, D., Toxuvo, T., KINZLER, K., VOGELSTEIN, B. and JACKS, T., 1995, p53-dependent and independent expression of p21 during cell growth, differentiation and DNA damage. Genes and Development, 9, 935–944.
   PubMed Web of Science ®Google Scholar
• MAY, E., JENKINS, J. R. and MAY, P., 1991, Endogenous HeLa p53 proteins are easily detected in HeLa cells transfected with mouse deletion mutant p53 gene. Oncogene, 6, 1363–1365.
   PubMed Web of Science ®Google Scholar
• METTING, N. F. and LITTLE, J. B., 1995, Transient failure to dephosphorylate the cdc2-cyclin B1 complex accompanies radiation-induced G2-phase arrest in HeLa cells. Radiation Research, 143, 286–292.
   PubMed Web of Science ®Google Scholar
• MILLER, A. C., GAFNER, J., CLARK, E. P. and SAMI DD., 1993a, Differences in radiation-induced micronuclei yields of human cells: influence of ras gene expression and protein localization. International Journal of Radiation Biology, 64, 547–554.
   PubMed Web of Science ®Google Scholar
• MILLER, A. C., KAIUKO, K., MYERS, C. E., CLARK, E. P. and SAMI DD., 1993b, Increased raclioresistance of EJras-transformed human osteosarcoma cells and its modulation by lovastatin, an inhibitor of p21 ras isoprenylation. International Journal of Cancer, 53, 302–307.
   PubMed Web of Science ®Google Scholar
• MILLER, A. C. and SAMI DD., 1995, Tumor resistance to oxidative stress: association with ras oncogene expression and reversal by lovastatin, an inhibitor of p2lras isoprenylation. International Journal of Cancer, 60, 249–254.
   PubMed Web of Science ®Google Scholar
• MIRANDA, E. I., SANTANA, C., ROJAS, E., HERNANDEZ, S., OSTROSKY-WEGMAN, P. and GARCIA-CARRANCA, A., 1996, Induced mitotic death of HeLa cells by abnormal expression of c-H-ras. Mutation Research, 349, 173–182.
   Google Scholar
• Ocus, K. and KAINA, B., 2000, Apoptosis induced by DNA damage 06-methylguanine is Bc1-2 and caspase-9/3 regulated and Fas/caspase-8 independent. Cancer Research, 60, 5815–5824.
   PubMed Web of Science ®Google Scholar
• PERONA, R., MONTANER, S., SANIGER, L., SANCHEZ-PEREZ, I., BRAVO, R. and LACAL, J. C., 1997, Activation of the nuclear factor kappaB by Rho, Cdc42 and Racl proteins. Genes and Development, 11, 463–475.
   PubMed Web of Science ®Google Scholar
• PINES, J., 1993, Cyclins and cycfin-dependent kinases: take your partners. Trends in Biochemical Science, 18, 195–197.
   PubMed Web of Science ®Google Scholar
• POON, R. Y., TOYOSHIMA, H. and HUNTER, T., 1995, Redistribution of the CDK inhibitor p27 between different cyclin. CDK complexes in the mouse fibroblast cell cycle and in cells arrested with lovastatin or ultraviolet irradiation. Molecular Biology of the Cell, 6, 1197–1213.
   PubMed Web of Science ®Google Scholar
• RAO, S., LOWE, M., HERLICZEK, T. W. and KEYOMARSI, K., 1998, Lovastatin mediated G1 arrest in normal and tumor breast cells is through inhibition of CDK2 activity and redistribution of p21 and p27, independent of p53. Oncogene, 17, 2393–2402.
   PubMed Web of Science ®Google Scholar
• RAO, S., PORTER, D. C., CHEN, X., HERLICZEK, T., LOWE, M. and KEYOMARSI, K., 1999, Lovastatin-mediated G1 arrest in through inhibition of the proteasome, independent of hyclroxymethyl glutaryl-CoA reductase. Biochemistry, 14, 7797–7802.
   Google Scholar
• REED, S. I., BArux, E., DuLic, V., HENGST, L., REsNrrzKY, D. and SLINGERIAND, J., 1994, G-1 control in mammalian cells. Journal of Cell Science, Supplement, 18, 69–73.
   PubMedGoogle Scholar
• ROSETTE, C. and KARIN, M., 1996, Ultraviolet light and osmotic stress: activation of the JNK cascade through multiple growth factor and cytokine receptors. Science, 274, 1194–1197.
   PubMed Web of Science ®Google Scholar
• SACHSENMAIER, C., RADLER-POHL, A., ZINCK, R., NORDHEIM, A., HERRLICH, P. and RAHMSDORF, H. J., 1994, Involvement of growth factor receptors in the mammalian UVC response. Cell, 78, 963–972.
   PubMed Web of Science ®Google Scholar
• SAMID, D., MILLER, A. C., RIMOLDI, D., GAFNER, J. and CLARK, E. P., 1991, Increased radiation resistance in transformed and nontransformed cells with elevated ras proto-oncogene expression. Radiation Research, 126, 244–250.
   PubMed Web of Science ®Google Scholar
• SANTANA, C., ORTEGA, E. and GARCIA-CARRANCA, A., 2002, Oncogenic H-ras induces cyclin B1 expression in a p53-independent manner. Mutation Research, 508, 49–58.
   Google Scholar
• SHAULIAN, E., SCHREIBER, M., PIU, F., BEECHE, M., WAGNER ERWIN, F. and KARIN, M., 2000, The mammalian UV response: c-Jun induction is required for exit from p53-imposed growth arrest. Cell, 103, 897–907.
   PubMed Web of Science ®Google Scholar
• SINGH, N. P., McCoY, M. T., TICE, R. R. and SCHNEIDER, E. L., 1988, A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175, 184–191.
   PubMed Web of Science ®Google Scholar
• TAMAMOTO, T., OHNISHI, K., TAKAHASHI, A., WANG, X., YOSIMURA, H., Omsm, H., UCHEDA, H. and OHNism, T., 1999, Correlation between gamma-ray-induced G2 arrest and raclioresistance in two human cancer cells. International Journal Radiation Oncology Biology Physics, 44, 905–909.
   PubMed Web of Science ®Google Scholar
• TEYSSIER, F., BAY JACQUES, 0., DIONET, C. and VERRFTJY, P., 1999, Cell cycle regulation after exposure to ionizing radiation. Bulletin du Cancer Montrouge, 86, 345–357.
   Google Scholar
• THERON, T., BINDER, A., VERHEYE DUA, F. and Boum, L., 2000, The role of G2-block abrogation, DNA double-strand break repair and apoptosis in the radiosensitization of melanoma and squamous cell carcinoma cell lines by pentoxifylline. International Journal of Radiation Biology, 76, 1197–1208.
   PubMed Web of Science ®Google Scholar
• VERHEIJ, M., BOSE, R., UN MK, H., YAO, B., JARvis, W. D., GRANT, S., BIRRER, M. J., SZABO, E., ZON, L. I., KyRudus, J. M., HAlmovrrz FRIEDMAN, A., FuKs, Z. and KOLESNICK, R., N., 1996, Requirement for ceramide-initiated SAPK/ JNK signalling in stress-induced apoptosis. Nature, 380, 75–79.
   PubMed Web of Science ®Google Scholar
• VIDAL, A., MILLARD, S. S., MILLER," P. and KOFF, A., 2002, Rho activity can alter the translation of p27 mRNA and is important for RasV12-induced transformation in a manner dependent on p27 status. Journal of Biological Chemistry, 277, 16433–16440.
   Google Scholar
• VON BARDELEBEN, R., DUNKERN, T., KAINA, B. and FRITZ, G., 2002, The HMG-CoA reductase inhibitor lovastatin protects cells from the antineoplastic drugs doxorubicin and etoposide. International Journal of Molecular Medicine, 10, 473–479.
   PubMed Web of Science ®Google Scholar
• WANG, C. Y., MAYO, M. W. and BALDWIN, JR, A. S., 1996, TNF and cancer therapy-induced apoptosis: potentiation by inhibition of NF-KB. Science, 274, 784–786.
   PubMed Web of Science ®Google Scholar
• WANG, S., GUO, M., OUYANG, H., Li, X., CORDON CARDO, C., KURIMASA, A., CHENDAVID, J., FuKs, Z., LING, C. C. and LI GLORIA, C. Proceedings of the National Academy of Sciences, USA, 97, 1584-1588.
   Google Scholar
• Woo, R. A., McLuRE, K. G., LEES-MILLER, S. P., RANCOURT, D. E. and LEE, P. W. K., 1998, DNA-dependent protein kinase acts upstream of p.53 in response to DNA damage. Nature, 394, 700–704.
   Google Scholar
• WRIGHT, J. A., KEEGAN, K. S., HERENDEEN, D. R., BENTLEY, N. J., CARR, A. M., HOEKSTRA, M. F. and CONCANNON, P., 1998, Protein kinase mutants of human ATR increase sensitivity to UV and ionizing radiation and abrogate cell cycle checkpoint control. Proceedings of the National Academy of Sciences, USA, 95, 7445–7450.
   PubMed Web of Science ®Google Scholar
• XTA, Z., DICKENS, M., RAINGEAUD, J., DAVIS, R. J. and GREENBERG, M. E., 1995, Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science, 270, 1326–1331.
   PubMed Web of Science ®Google Scholar
• Xu, X. Q, McGuIRE, T. F., BLASKOWCH, M. A., SEBTI, S. M. and ROMERO, G., 1996, Lovastatin inhibits the stimulation of mitogen-activated protein kinase by insulin in HIRcB fibroblasts. Archives of Biochemical and Biophysics, 326, 233–237.
   PubMed Web of Science ®Google Scholar