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Original Article

The Induction of DNA Double-strand Breaks in CHO Cells by Pvu II: Kinetics Using Neutral Filter Elution (pH 9·6)

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Pages 933-938 | Received 31 Aug 1989, Accepted 29 Dec 1989, Published online: 03 Jul 2009

References

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  • Natarajan A.T., Mullenders L.H.F., Meijers M., Mukherjee U. Induction of sister-chromatid exchanges by restriction endonucleases. Mutation Research 1985; 144: 33–39
  • Obe G., Winkel E-U. The chromosome-breaking activity of the restriction endonuclease Alu I in CHO cells is independent of the S-phase of the cell cycle. Mutation Research 1985; 152: 25–29
  • Okayasu R., Iliakis G. Linear DNA elution dose response curves obtained in CHO cells with non-unwinding filter elution after appropriate selection of the lysis conditions. International Journal of Radiation Biology 1989; 55: 569–581
  • Winegar R.A., Preston R.J. The induction of chromosome aberrations by restriction endonucleases that produce blunt-end or cohesive-end double-strand breaks. Mutation Research 1988; 197: 141–149
  • Winegar R.A., Phillips J.W., Youngblom J.K., Morgan W.F. Cell electroporation of a highly efficient method for introducing restriction endonucleases into cells. Mutation Research 1989; 225: 49–53

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