34
Views
47
CrossRef citations to date
0
Altmetric
Original Article

Comparison of DNA Double-strand Break Rejoining as Measured by Pulsed Field Gel Electrophoresis, Neutral Sucrose Gradient Centrifugation and Non-unwinding Filter Elution in Irradiated Plateau-phase CHO Cells

, , &
Pages 927-939 | Received 10 Aug 1990, Accepted 30 Oct 1990, Published online: 03 Jul 2009

References

  • Blöcher D. DNA double strand breaks in Ehrlich tumor cells at low doses of X-rays: I. Determination of induced breaks by centrifugation at reduced speed. International Journal of Radiation Biology 1982; 42: 317–328
  • Blöcher D., Pohlit W. Double strand breaks in Ehrlich ascites tumor cells at low doses of X-rays. II. Can cell death be attributed to double-strand breaks?. International Journal of Radiation Biology 1982; 42: 329–338
  • Blöcher D., Nuesse M., Bryant P.E. Kinetics of double strand break repair in the DNA of X-irradiated synchronized mammalian cells. International Journal of Radiation Biology 1983; 43: 579–584
  • Blöcher D., Einspenner M., Zajackowski J. CHEF electrophoresis, a sensitive technique for the determination of DNA double-strand breaks. International Journal of Radiation Biology 1989; 56: 437–448
  • Bradley M.D., Kohn K.W. X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution. Nucleic Acids Research 1979; 7: 793–804
  • Bryant P.E., Blöcher D. The effects of 9-β-d-arabinofuranosyladenine on the repair of DNA strand breaks in X-irradiated Ehrlich ascites tumour cells. International Journal of Radiation Biology 1982; 42: 385–394
  • Coquerelle T.M., Weibezahn K.F., Lucke-Huhle C. Rejoining of double strand breaks in normal human and ataxia-telangiectasia fibroblasts after exposure to 60Co gamma-rays, 241Am alpha-particles or bleomycin. International Journal of Radiation Biology 1987; 51: 209–218
  • Cornforth M.N., Bedford J.S. On the nature of a defect in cells from individuals with ataxia-telangiectasia. Science 1985; 227: 1589–1591
  • Denko N., Peters B., Giaccia A., Stamato T. An asymmetric field inversion gel electrophoresis system for the separation of large DNAs. Analytical Biochemistry 1989; 178: 172–176
  • Frankenberg-Schwager M. Review of repair kinetics for DNA damage induced in eukaryotic cells in vitro by ionizing radiation. Radiotherapy and Oncology 1989; 14: 307–320
  • Iliakis G. Radiation-induced potentially lethal damage: DNA lesions susceptible to fixation. International Journal of Radiation Biology 1988; 53: 541–584
  • Iliakis G.E., Okayasu R. Radiosensitivity throughout the cell cycle and repair of potentially lethal damage and DNA double-strand breaks in an X-ray-sensitivie CHO mutant. International Journal of Radiation Biology 1990; 57: 1195–1211
  • Iliakis G., Pantelias G.E., Okayasu R., Seaner R. 125IdUrd induced chromosome fragments, assayed by premature chromosome condensation, and DNA double strand breaks have similar repair kinetics in G1-phase CHO cells. International Journal of Radiation Biology 1987; 52: 705–722
  • Iliakis G., Pantelias G.E., Seaner R. Effect of arabinofuranosyladenine on radiation-induced chromosome damage in plateau-phase CHO cells measured by premature chromosome condensation: implications for repair and fixation of alpha-PLD. Radiation Research 1988; 114: 361–378
  • Iliakis G.E., Metzger L., Denko N., Stamato T.D. Detection of DNA double strand breaks in synchronous cultures of CHNO cells by means of asymmetric field inversion gel electrophoresis. International Journal of Radiation Biology 1991; 59: 321–341
  • Koval T.M., Kazmar E.R. DNA double-strand break repair in eukaryotic cells lines having radiacally different radiosensitivities. Radiation Research 1988; 113: 268–277
  • Metzger, L., Iliakis, G. Detection of DNA double strand breaks by asymmetric field inversion gel electrophoresis: Repair kinetics throughout the cell cycle. International Journal of Radiation Biology, in press, 1991
  • Okayasu R., Iliakis G. Linear DNA elution dose response curves obtained in CHO cells with non-unwinding filter elution after appropriate selection of the lysis conditions. International Journal of Radiation Biology 1989; 55: 569–581
  • Okayasu R., Blöcher D., Iliakis G. Variation through the cell cycle of DNA neutral filter elution dose response in X-irradiated synchronous CHO cells. International Journal of Radiation Biology 1988; 53: 729–747
  • Pantelias G.E., Maillie H.D. A simple method for premature chromosome condensation induction in primary human and rodent cells using polyethylene glycol. Somatic Cellular Genetics 1983; 9: 533–548
  • Pantelias G.E., Maillie H.D. The use of peripheral blood mononuclear cell prematurely condensed chromosomes for biological dosimetry. Radiation Research 1984; 99: 140–150
  • Radford I.R. Effect of cell-cycle position and dose on the kinetics of DNA double-strand breakage repair in X-irradiated Chinese hamster cells. International Journal of Radiation Biology 1987; 52: 555–563
  • Radford I.R. Review. The dose-response for low-LET radiation-induced DNA double-strand breakage: methods of measurement and implications for radiation action models. International Journal of Radiation Biology 1988; 54: 1–11
  • Stamato T.D., Denko N. Asymmetric field inversion gel electrophoresis: a new method for detecting DNA double strand breaks in mammalian cells. Radiation Research 1990; 121: 196–205
  • Sweigert S.E., Eguchi-Kasai K., Warters R.L., Dethlefsen L.A. Repair of DNA single- and double-strand breaks in proliferating and quiescent murine tumor cells. International Journal of Radiation Biology 1989; 56: 253–264
  • vanAnkeren S.C., Murray D., Meyn R.E. Induction and rejoining of gamma-ray-induced DNA single- and double-strand breaks in Chinese hamster AA8 cells and in two radiosensitive clones. Radiation Research 1988; 116: 511–525
  • van der Schans G.P., Paterson M.C., Cross W.G. DNA strand breaks and rejoining in cultured human fibroblasts exposed to fast neutrons or gamma rays. International Journal of Radiation Biology 1983; 44: 75–85
  • Ward J.F. DNA damage produced by ionizing radiation in mammalian cells: identities, mechanisms of formation, and reparability. Progress in Nucleic Acid Research and Molecular Biology 1988; 35: 95–125
  • Weibezahn K.F., Coquerelle T. Radiation induced DNA double strand breaks are rejoined by ligation and recombination processes. Nucleic Acids Research 1981; 9: 3139–3150
  • Wlodek D., Hittelman W.N. The repair of double-strand DNA breaks correlates with radiosensitivity of L5178Y-S and L5178Y-R cells. Radiation Research 1987; 112: 146–155
  • Woods W.G. Quantitation of the repair of gamma-radiation-induced-double-strand DNA breaks in tumor fibroblasts. Biochemica et Biophysica Acta 1981; 655: 342–348

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.