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Original Article

The Filter Does Not Act as a DNA Size Discriminator in the Neutral Filter Elution Technique

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Pages 397-399 | Received 29 Aug 1991, Accepted 17 May 1992, Published online: 03 Jul 2009

References

  • Balbi C., Pala M., Parodi S., Figari G., Cavazza B., Trefiletti V., Patrone E. A simple model for elution from filters. Journal of Theoretical Biology 1986; 118: 183–198
  • Blöcher D. Dose response in neutral filter elution. Radiation Research 1990; 123: 178–181
  • Blöcher D., Iliakis G. Size distribution of DNA molecules recovered from non-denaturing filter elution. International Journal of Radiation Biology 1991; 59: 919–926
  • Bradley M.O., Kohn K.W. X-ray induced DNA double strand break production and repair as measured by neutral filter elution. Nucleic Acids Research 1979; 7: 793–804
  • Kohn K.W. DNA as target in chemotherapy: Measurement of macromolecular DNA damage produced in mammalian cells by anti-cancer agents and carcinogens. Methods in Cancer Research 1979; 16: 291–345
  • Mayer P.J., Lange C.S., Bradley M.O., Nichols W.W. Investigation of the neutral filter elution technique. Radiation Research 1991; 125: 197–205
  • Nicolini C., Belmont A., Zietz S., Maura A., Pinto A., Robbiano L., Brambilla G. Physico-chemical model for DNA alkaline elution: New experimental evidence and differential role of DNA length, chain flexibility and superpacking. Journal of Theoretical Biology 1983; 100: 341–357
  • Okayasu R., Iliakis G. Linear DNA elution dose response curves obtained in CHO cells with non-unwinding filter elution after appropriate selection of the lysis conditions. International Journal of Radiation Biology 1989; 55: 561–581
  • Radford I.R. The level of induced DNA double-strand breakage correlates with cell killing after X-irradiation. International Journal of Radiation Biology 1985; 48: 45–54
  • Radford I.R., Hodgson G.S. 125I-induced DNA double strand breaks: use in calibration of the neutral filter elution technique and comparison with X-ray induced Breaks. International Journal of Radiation Biology 1985; 48: 555–566
  • Wlodek D., Olive P.L. Physical basis for detection of DNA double-strand breaks using neutral filter elution. Radiation Research 1990; 124: 326–333

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