An Automated Flow Cytometric Micronucleus Assay for Human Lymphocytes

References

• Bauchinger M., Braselmann H. Use of micronuclei in biological dosimetry of absorbed radiation dose. Chromosomal Aberrations, Basic and Applied Aspects, G. Obe, A.T. Natarajan. Springer, Berlin Heidelberg 1989; 202–211, In
   Google Scholar
• Boehmer R.W., Ellwart J. Cell cycle analysis by combining the 5-bromodeoxyuridine/33258 Hoechst technique with DNA-specific ethidium bromide staining. Cytometry 1981; 2(1)31–31
   PubMedGoogle Scholar
• Brodie S., Giron J., Latt S.A. Estimation of accessibility of DNA in chromatin from fluorescence measurements of electronic excitation energy transfer. Nature 1975; 253: 470–471
   PubMed Web of Science ®Google Scholar
• Callisen H., Norman A., Pincu M. Computer scoring of micronuclei in human lymphocytes. Biological Dosimetry, W.G. Eisert, M.L. Mendelsohn. Springer, Berlin Heidelberg 1984; 171–179, In
   Google Scholar
• Callisen H.H., Pincu M., Norman A. Feasibility of automating the micronucleus assay. Analytical and Quantitative Cytology and Histology 1986; 8: 219–223
   PubMedGoogle Scholar
• Countrytman P.I., Heddle J.A. The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes. Mutation Research 1976; 41: 321–332
   PubMedGoogle Scholar
• Countrytman P.I., Heddle J.A., Crawford E. The repair of X-ray-induced chromosomal damage in trisomy 21 and normal diploid lymphocytes. Cancer Research 1977; 37: 52–58
   PubMedGoogle Scholar
• Fenech M., Morley A.A. Solutions to the kinetic problem in the micronucleus assay. Cytobios 1985a; 43: 233–246
   PubMedGoogle Scholar
• Fenech M., Morley A.A. Measurement of micronuclei in lymphocytes. Mutation Research 1985b; 147: 29–36
   PubMed Web of Science ®Google Scholar
• Fenech M., Morley A.A. The effect of donor age on spontaneous and induced micronuclei. Mutation Research 1985c; 148: 99–105
   PubMed Web of Science ®Google Scholar
• Fenech M., Morley A.A. Cytokinesis-block micronucleus method in human lymphocytes: effect of in vivo ageing and low dose X-irradiation. Mutation Research 1986; 161: 193–198
   PubMed Web of Science ®Google Scholar
• Fenech M., Morley A.A. Ageing in vivo does not influence micronucleus induction in human lymphocytes by X-irradiation. Mechanisms of Ageing and Development 1987; 39: 113–119
   PubMedGoogle Scholar
• Fenech M., Jarvis L.R., Morley A.A. Preliminary studies on scoring micronuclei by computerised image analysis. Mutation Research 1988; 203: 33–38
   PubMed Web of Science ®Google Scholar
• Högstedt B. Micronuclei in lymphocytes with preserved cytoplasm. A method for assessment of cytogenetic damage in man. Mutation Research 1984; 130: 63–72
   PubMedGoogle Scholar
• Huber R., Braselmann H., Bauchinger M. Screening for interindividual differences in radiosensitivity by means of the micronucleus assays in human lymphocytes. Radiation and Environmental Biophysics 1989; 28: 113–120
   PubMed Web of Science ®Google Scholar
• Huber R., Bauchinger M. Development and perspectives of the human lymphocyte micronucleus assay. Advances in Mutagenesis Research 1, G. Obe. Springer, Berlin Heidelberg 1990; 89–104, In
   Google Scholar
• Huber R., Braselmann H., Bauchinger M. Intra- and interindividual variation of background and radiation-induced micronucleus frequencies in human lymphocytes. International Journal of Radiation Biology 1992, in press
   PubMedGoogle Scholar
• Ihnen L.A., Goodnight J.H. The NLIN procedure. SAS Users Guide: Statistics. Cary, NC, SAS Institute Inc. 1982, In
   Google Scholar
• Kellerer A.M., Brenot J. On the statistical evaluation of dose-response functions. Radiation and Environmental Biophysics 1974; 11: 1–13
   PubMed Web of Science ®Google Scholar
• Kubbies M., Rabinovitch P.S. Flow cytometric analysis of factors which influence the BrdUrd-Hoechst quenching effect in cultivated human fibroblasts and lymphocytes. Cytometry 1983; 3: 276–281
   PubMedGoogle Scholar
• Kubbies M., Friedl R., Koehler J., Rabinovitch P.S., Hoehn H. Improvement of human lymphocyte proliferation and alteration of IL-2 secretion kinetics by alpha-thioglycerol. Lymphokine Research 1990; 9: 95–106
   PubMedGoogle Scholar
• Merkle W. Statistical methods in regression and calibration analysis of chromosome aberration data. Radiation and Environmental Biophysics 1983; 21: 217–233
   PubMed Web of Science ®Google Scholar
• Miller R.G., Jr. Simultaneous Statistical Inference2nd edn. Springer Verlag, New York 1982
   Google Scholar
• Norman A., Cochran S., Bass D., Roe D. Effects of age, sex and diagnostic X-ray on chromosome damage. International Journal of Radiation Biology 1984; 46: 317–321
   Google Scholar
• Norman A., Bass D., Roe D. Screening human population for chromosome aberrations. Mutation Research 1985; 143: 155–160
   PubMed Web of Science ®Google Scholar
• Nüsse M., Kramer J. Flow cytometric analysis of micronuclei found in cells after irradiation. Cytometry 1984; 5: 20–25
   PubMedGoogle Scholar
• Nüsse M., Egner H.J., Krämer M. Flow cytometric detection of chromosome aberrations and micronucleus induction in irradiated V79 cells. International Journal of Radiation Biology 1987a; 51: 910–911
   Google Scholar
• Nüsse M., Krämer M., Viaggi S., Bartsch A., Bonatti S. Anti-kinetochore antibodies and flow karyotyping: new techniques to detect aneuploidy in mammalian cells induced by ionizing radiation and chemicals. Molecular Toxicology 1987b; 1: 393–405
   PubMedGoogle Scholar
• The Generalized Linear Interactive Modelling (GLIM) System, Release 3.77, Manual, C.D. Payne. Numerical Algorithms Group (NAG), Oxford 1985
   Google Scholar
• Pincu M., Bass D., Norman A. An improved micronucleus assay in lymphocytes. Mutation Research 1984; 139: 61–65
   PubMed Web of Science ®Google Scholar
• Prosser J.S., Moquet E., Lloyd D.C., Edwards A.A. Radiation induction of micronuclei in human lymphocytes. Mutation Research 1988; 199: 37–45
   PubMed Web of Science ®Google Scholar
• Schreiber G.A., Beisker W., Bauchinger M., Nüsse M. Multiparametric flow cytometric analysis of radiation-induced micronuclei in mammalian cell culturs. Cytometry 1992; 13: 90–102
   PubMedGoogle Scholar
• Tates A.D., van Welie M., Ploem J.S. The present state of the automated micronucleus test for lymphocytes. International Journal of Radiation Biology 1990; 58: 813–825
   PubMed Web of Science ®Google Scholar
• Thierens H., Vral A., de Ridder L. Biological dosimetry using the micronucleus assay for lymphocytes: interindividual differences in dose response. Health Physics 1991; 61: 623–630
   PubMed Web of Science ®Google Scholar