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Original Article

Application of Programmable, Autonomously Controlled Electrode (PACE) Technology to the Development of an Improved Pulsed Field Gel Electrophoresis Assay for DNA Double-strand Breaks in Mammalian Cells

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Pages 7-11 | Received 10 Mar 1992, Accepted 23 Jul 1992, Published online: 03 Jul 2009

References

  • Ager D.D., Dewey W.C., Gardner K., Harvey W., Johnson R.T., Waldren C.A. Measurement of radiation-induced DNA double-strand breaks by pulsed-field gel electrophoresis. Radiation Research 1990; 122: 181–187
  • Ahn S.Y., Nevaldine B., Hahn P.J. Direct measurement by pulsed-field gel electrophoresis of induction and rejoining of X-ray-induced double-strand breaks in cultured mouse cells. International Journal of Radiation Biology 1991; 59: 661–675
  • Birren B.W., Lai E., Clark S.M., Hood L., Simon M.I. Optimized conditions for pulsed field gel electrophoretic separations of DNA. Nucleic Acids Research 1988; 16: 7563–7582
  • Birren B.W., Hood L., Lai E. Pulsed field gel electrophoresis: studies of DNA migration made with the programmable, autonomously-controlled electrode electrophoresis system. Electrophoresis 1989; 10: 302–309
  • Blöcher D., Kuhni M. DNA double-strand break analysis by CHEF electrophoresis. International Journal of Radiation Biology 1990; 58: 23–24
  • Clark S.M., Lai E., Birren B.W., Hood L. A novel instrument for separating large DNA molecules with pulsed homogenous electric fields. Science 1988; 241: 1203–1205
  • Elia M.C., Bradley M.O. Influence of chromatin structure on the induction of DNA double strand breaks by ionizing radiation. Cancer Research 1992; 52: 1580–1586
  • Elia M.C., DeLuca J.G., Bradley M.O. Significance and measurement of DNA double strand breaks in mammalian cells. Pharmacology and Therapeutics 1991; 51: 291–327
  • Elia M.C., Harmon L.S., Kraynak A.R., McKelvey T.W., Hertzog P.R., Keenan K.P., DeLuca J.G., Storer R.D., Nichols W.W. Cytotoxicity as measured by trypan blue as a potentially confounding variable in the in vitro alkaline elution/rat hepatocyte assay. Mutation Research, (in press)
  • Iliakis G.E., Metzger L., Denko N., Stamato T.D. Detection of DNA double-strand breaks in synchronous cultures of CHO cells by means of asymmetric field inversion gel electrophoresis. International Journal of Radiation Biology 1991a; 59: 321–341
  • Iliakis G.E., Cicilioni O., Metzger L. Measurement of DNA double-strand breaks in CHO cells at various stages of the cells cycle using pulsed field gel electrophoresis: calibration by means of 125I decay. International Journal of Radiation Biology 1991b; 59: 343–357
  • Smith C.L., Matsumoto T., Niwa O., Klco S., Fan J.-B., Yanagida M., Cantor C.R. An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis. Nucleic Acids Research 1987; 15: 4481–4489
  • Stamato T.D., Denko N. Asymmetric field inversion gel electrophoresis: a new method for detecting DNA double-strand breaks in mammalian cells. Radiation Research 1990; 121: 196–205
  • Steele P.E., Carle G.F., Kobayashi G.S., Medoff G. Electrophoretic analysis of Histoplasma capsulatum chromosomal DNA. Molecular and Cellular Biology 1989; 9: 983–987
  • Vollrath D., Davis R.W. Resolution of DNA molecules greater than 5 Mb by contour-clamped homogenous electric fields. Nucleic Acids Research 1987; 15: 7865–7876
  • Wlodek D., Banath J., Olive P.L. Comparison between pulsed-field and constant-field gel electrophoresis for measurement of DNA double-strand breaks in irradiated Chinese hamster ovary cells. International Journal of Radiation Biology 1991; 60: 779–790

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