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Original Article

Flow Cytometric Analysis of Micronuclei in the CD2 ± Subpopulation of Human Lymphocytes Enriched by Magnetic Separation

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Pages 193-202 | Received 18 Apr 1994, Accepted 27 Sep 1994, Published online: 03 Jul 2009

References

  • Ban S., Cologne J.B. X-ray induction of micronuclei in human lymphocyte subpopulations differentiated by immunoperoxidase staining. Radiation Research 1992; 131: 60–65
  • Ban S., Cologne J.B., Fujita S., Awa A.A. Radiosensitivity of atomic bomb survivors as determined with a micronucleus assay. Radiation Research 1993; 134: 170–178
  • Cao J., Leibold E., Beisker W., Schranner T., Nüsse M., Schwarz L. Flow cytometric analysis of in vitro micronucleus induction in hepatocytes treated with carcinogens. Toxicology In Vitro 1993; 7: 447–451
  • Castelain PH., Van Hummelen P., Deleener A., Kirsch-Volders M. Automated detection of cytochalasin-B binucleated lymphocytes for scoring micronuclei. Mutagenesis 1993; 8: 285–293
  • Countryman P.I., Heddle J.A. The production of micronuclei from chromosome aberrations in irradiated cultures of human lymphocytes. Mutation Research 1976; 410: 321–332
  • Fenech M. The cytokinesis-block micronucleus technique: a detailed description of the method and its application to genotoxicity studies in human population. Mutation Research 1993; 285: 35–44
  • Fenech M., Morley A.A. Measurement of micronuclei in lymphocytes. Mutation Research 1985a; 147: 29–36
  • Fenech M., Morley A.A. The effect of donor age on spontaneous and induced micronuclei. Mutation Research 1985 b; 148: 99–105
  • Fenech M., Jarvis L.R., Morley A.A. Preliminary studies on scoring micronuclei by computerised image analysis. Mutation Research 1988; 203: 33–38
  • Gantenberg H.-W., Wuttke K., Streffer C., Müller W.-U. Micronuclei in human lymphocytes irradiated in vitro or in vivo. Radiation Research 1991; 128: 276–281
  • Heddle J.A., Carrano A.A. The DNA content of micronuclei induced in mouse bone marrow by gamma-irradiation: evidence that micronuclei arise from acentric chromosomal fragments. Mutation Research 1977; 44: 63–69
  • Hogstedt B., Karlsson A., Bratt I., Holmen A. Micronuclei induction in human B and T lymphocytes separated by an immunomagnetic technique. Hereditas 1993; 119: 99–103
  • Huber R., Braselmann H., Bauchinger M. Screening for inter-individual differences in radiosensitivity by means of the micronucleus assay in human lymphocytes. Radiation and Environmental Biophysics 1989; 28: 113–120
  • Huber R., Braselmann H., Bauchinger M. Intra- and interindividual variation of background and radiation-induced micronucleus frequencies in human lymphocytes. International Journal of Radiation Biology 1992; 61: 655–661
  • Iskandar O. An improved method for the detection of micronuclei in human lymphocytes. Stain Technology 1979; 54: 221–223
  • Kubbies M., Friedl R., Koehler J., Rabinovich P.S., Hoehn H. Improvement of human lymphocyte proliferation and alteration of IL-2 secretion kinetics by alpha-thioglycerol. Lymphokine Research 1990; 9: 95–106
  • Ludwikow G., Stalnacke C.G., Johnson K.J., Sundell-Bergman S., Richter S. Microscopic and flow cytometric study of micronuclei in iododeoxyuridine labelled cells irradiated with soft X-rays. Acta Oncologica 1990; 29: 761–767
  • Mills G.B., Lee J.W.W., Cheung R.K., Gelfand E.W. Characterization of the requirements for human T cell mitogenesis by using suboptimal concentrations of phytohemagglutinin. Journal of Immunology 1985; 135: 3087–3093
  • Minon J.M., Sequaris M., Bouillenne C., André A. Determination des valeurs normales des souspopulations lymphocytaires T par cytofluorometrie de flux. Annales Biologie Clinique 1987; 45: 431–433
  • Nüsse M., Kramer J. Flow cytometric analysis of micronuclei found in cells after irradiation. Cytometry 1984; 5: 20–25
  • Nüsse M., Recknagel S., Beisker W. Micronuclei induced by 2-chlorobenzylidene malonitrite contain single chromosomes as demonstrated by the combined use of flow cytometry and immunofluorescent staining with antikinetochore antibodies. Mutagenesis 1992a; 7: 57–67
  • Nüsse M., Kramer J., Miller B.M. Factors influencing the DNA content of radiation-induced micronuclei. International Journal of Radiation Biology 1992 b; 62: 587–602
  • Pincu M., Calissen H., Norman A. DNA content of micronuclei in human lymphocytes. International Journal of Radiation Biology 1985; 47: 423–432
  • Prosser J.S., Moquet J.E., Lloyd D.C., Edwards A.A. Radiation induction of micronuclei in human lymphocytes. Mutation Research 1988; 199: 37–45
  • Schreiber G.A., Beisker W., Braselman H., Bauchinger M., Boegl K.W., Nüsse M. An automated flow cytometric micronucleus assay for human lymphocytes. International Journal of Radiation Biology 1992 b; 62: 695–709
  • Schreiber G.A., Beisker W., Bauchinger M., Nüsse M. Multiparametric flow cytometric analysis of radiation-induced micronuclei in mammalian cell cultures. Cytometry 1992 a; 13: 90–102
  • Tates A.D., Van Welie M., Ploem J.S. The present state of the automated micronucleus test for lymphocytes. International Journal of Radiation Biology 1990; 58: 813–825
  • Weller E.M., Dietrich I., Viaggi S., Beisker W., Nüsse M. Flow cytometric analysis of bromodeoxyuridine-induced micronuclei. Mutagenesis 1993; 8: 437–444
  • Wuttke K., Streffer C., Müller W.-U. Radiation-induced micronuclei in subpopulations of human lymphocytes. Mutation Research 1993; 286: 181–188

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