Advanced search
Publication Cover
International Journal of Radiation Biology Volume 75, 1999 - Issue 4
Submit an article Journal homepage
32
Views
20
CrossRef citations to date
0
Altmetric
Research Article

Multicolour FISH painting for the analysis of chromosomal aberrations induced by 220kV X-rays and fission neutrons

S. KNEHR GSF-National Research Center for Environment and Health,Institute of Radiobiology and Institute of Radiation Protection,Institute of Radiobiology, Ingolstadter Landstr. 1, 85 758 Oberschleiheim, Germany.
Pages 407-418 | Published online: 03 Jul 2009

  • BARQUINERO, J. F., KNEHER, S., BRASELMANN, H., EIGEL., M. and BACHINGER, M., 1998, DNA-proportioanl distrbution of radidation-induced chromosome aberrarons analyzed by flourescence in situ hybirdisation (FISH)-painting of all chromosomes of a human female karyotype. International Journal of Radiation Biology, 74, 315- 323.
  • BAUCHINGER, M.,. SCHMID, E., ZITZELSBERGER, 11., BRASELMANN, h., and NAHRSTEDT, U., 1993, Radiationinduced chromosome aberrationsanalyzed by two-color flourescence in situ hybirdization with composite whole chromosome-specific DNA-probes and a pancentromeric DNA-probe. International Journal of Radiation Biology, 64, 179-184.
  • BOEL, J. J. W. A., VERMEULEN, S., FOMINA, J. NATARAJAN, A. T., 1998, Detection of incomplete exchanges and interstitial fragments in X-irradiated human lymphocytes using a telomeric PNA probe. International Journal of Radiation Biology, 73, 599-603.
  • FINNON, P., LLOYD, D. c. and EDWARDS. A. A., 1995, Fluorescence, in situ hybirdization detection of chromosomal aberrations in human lymphocytes: applicability to biological dosimetry. International Journal of Radiation Biology, 68, 429-435.
  • RIFFIN, C. S. MARSDEN, S. J., STEVENS, D.L., SIMPSON, P. and SAVAGE, J. R. K., 1995, Frequencies of complex chromosome exchange aberrations induced 238 Pu aparticles and detected by fluorescence in situ hybirdization using single chromosome-specific probes. Internatioanal Journal of Radiation Biology, 67, 431-439.
  • HUBER, R., SCHRAUBE, H., NAHRSTEDT, U., BRASELMANN, H. and BAUCHINGER, M., 1994, Dosc response relationships of micronuclei in human lymphocytes induced by fission neutrons and by low LET radiations. Mutation Research, 306, 135- 141.
  • JOHNSON, G. D. and DE C. NOGUEIRA ARAUJO, G. M., 1981, A simple method of reducing the fading of immunofluorescence during microscopy. Immunological Methods, 43, 349-350.
  • KNEHR, S., ZITZELSBERGER, H. and BAUCHINGER, M., 1998, FISH-based analysis of radiation-induced chromosomal aberrations using different nomenclature systems. International Journal of Radiation Biology, 73, 135-141.
  • KULKA, U., HUBER, R., MüLLER, P., KNEHR, S. and BAUCHINGER, M., 1995, Combined FISH-painting and harlequin staining for cell cycle-controlled chromosome analysis in human lymphocytes. International Journal of Radiation Biology, 68, 25-27.
  • LUCAS, J. N., AWA, A., STRAUME, T., POGGENSEE, M., KODAMA, Y., NAKANO, M., OHTAKI, K., WEIER, H.-U., PINKEL, D., GRAY, J. and LITTLEFIELD, G., 1992., Rapid translocation frequency analysis in man decades after exposure to ionizing radiation. International Journal of Radiation Biology, 62, 53-63.
  • MORTON, N. E., 1991, Parameters of the human get genome, Proceedings of the National Academy of Sciences. USA, 88, 7474-7476.
  • PAPWORTH, D., 1975, Appendix, in Savage, J. R. K., Radiation induced chromosomal aberrations in the plant Tradescantia. Radiation Botany, 15, 87- 140.
  • SAVAGE, J. R. K. and SIMPSON, P. J., 1994a, On the scoring of FISH-'painted' chromosome exchange aberrations. Mutation Research, 307, 345-353.
  • SAVAGE, J. R. K. and SIMPSON, P. J., 1994b, FISH 'painting' patterns resulting from complex exchange. Mutation Research, 312, 51-60.
  • SAVAGE, J. R. K. and TUCKER, J. D, 1996, Nomenclature systems for FISH-painted chromsome aberrations. Mutation Research, 366, 153-161.
  • SCHMID, E., BAUCHINGER, M., STRENG, S. and NAHRSTEDT, U., 1984, The effect of 220kVp X-rays with different spectra on the dose response of chromsome aberrations in human lymphocytes. Radiation and Environmental Biophysics. 23, 305-309.
  • SCHMID, E., SCHAUBE, H. and BAUCHINGER, M., 1998, Chromosome aberraion frequencies in human lymphocytes irradiated in a phantom by a mixed beam of fission neutrons and ?-rays. International Journal of Radiation Biology, 73, 263-267.
  • SCHMID, E., ZITZELSBERGER, H., BRASELMANN, H., GRAY, J. W. and BAUCHINGER, M., 1992, Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA-probes. International Journal of Radiation Biology, 62, 673-678.
  • SIMPSON, P. J. and SAVAGE J. R. K., 1994, Identification of complex aberrations induced by X-rays using fluorescence in situ hybridization: a comparison at two doses. International Journal of Radiation Biology, 66, 629-632.
  • SIMPSON, P. J. and SAVAGE J. R. K., 1995a, Detecting 'hidden' exchange events within X-ray induced aberrations using multicolor chromosome paints. Chromosome Research, 3, 69-72.
  • SIMPSON, P. J. and SAVAGE., J. R. K., 1995b, Estimating the true frequency of X-ray induced complex chromosome exchanges using fluorescence in situ hybirdization. International Journal of Radiation Biology, 67, 37-45.
  • SIMPSON, P. J. and SAVAGE J. R. K., 1996, Dose response curves for simple and complex chromosome aberrations induced by X-rays and detected using fluorescence in situ hybirdization. International Journal of Radiation Biology, 69, 429-436.
  • TUCKER, J. D., LEE, C. A. and MOOREH, D. H., 1995a, Validation of chromosome painting. H. A detailed analysis of aberrations following high doses of ionizing radiation in vitro. International Journal of Radiation Biology, 67, 19-28.
  • TUCKER, J. D., morgan, W. F., AWA, A. A., BAUCHINGER, M., BLAKEY, D., CORNFORTH, M. N., LITTLEFIELD, L. G., NATARAJAN, A. T. and SHASSERRE, C., 1995b, A proposed system for scoring structural aberrations detected by chromosome painting. Cytogenetics and Cell Genetics, 68, 211-221.
  • WEIER, H.-U. G., LUCAS, J. N., POGGENSEE, M., SEGRAVES, R., PINKEL, D. and GRAY, J. W., 1991, Two-color hybirdization with high complexity chromosome-specific probes and a degenerate alpha-satellite probe DNA allows unambiguous discrimination between symmetrical and asymmetrical translocations. Chroniosonia, 100, 371-376.
  • WU, H., GEORGE, K. and YANG, T. C., 1998, Estimate of true incomplete exchanges using fluorecence in situ hybirdization with telomere probes. International Journal of Radiation Biology, 73, 521-527.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.