Amino acid residues N450 and Q449 are critical for the uptake capacity and specificity of UapA, a prototype of a nucleobase-ascorbate transporter family

References

• Bloch, J. C., Sychrova, H., Souciet, J. L., Jund, R. and Chevallier, M. R., 1992, Determination of a specific region of the purine-cytosine permease involved in the recognition of its substrates. Molecular Microbiology, 6, 2989–2997.
   PubMedGoogle Scholar
• Cove, D. J., 1966, The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et Biophysica Acta, 113, 51–56.
   PubMed Web of Science ®Google Scholar
• Darlington, A. J. and Scazzocchio, C., 1967, Use of analogues and the substrate specificity of mutants in analysis of purine uptake and breakdown in Aspergillus nidulans. Journal of Ba cteriology, 93, 937–940.
   PubMed Web of Science ®Google Scholar
• Diallinas, G. and Scazzocchio, C., 1989, A gene coding for the uric acid-xanthine permease of As pergillus nidulans: inactivational cloning, characterisation and sequence of a cis-acting mutation. Genetics, 122, 341–350.
   PubMed Web of Science ®Google Scholar
• Diallinas, G., Gorfinkiel, L., Arst, H. N. Jr., Ceccheto, G. and Scazzochio, C., 1995, Genetic and molecular characterisatio n of a gene encoding a wide specificity purine permease of Aspergillus nidulans reveals a novel family of transporters conserved in prokaryotes and eukaryotes. Journal of Biological Chemistry, 270, 8610–8622.
   PubMed Web of Science ®Google Scholar
• Diallinas G., Valdez, J., Sophianopoulou, V., Rosa, A. and Scazzocchio, C., 1998, Chimeric protein analysis reveals a region involved in function and specificity of purine transporters in the filamentous fungus Aspergillus nidulans conserved in bacteria, plants and metazoans. E uropean Mole cular Biology Organisation Journal, 17, 3827–3837.
   PubMedGoogle Scholar
• Erion, M. D., Takabayashi, K., Smith, H. B., Kessi, J., Wagner, S., Honger, S., Shames, S. L. and Ealick, S. E., 1997, Purine nucleoside phosphorylase. 1. Structure-function studies. Bio-chemis try, 30, 11725–11734.
   Google Scholar
• Faaland, C. A., Race, J. E., Ricken, G., Warner, F. J., Williams, W. J. and Holtzman, E. J., 1998, Molecular characterisation of two novel transporters from human and mouse kidney and from LLC-PK1 cells reveals a novel family that is homologous to bacterial and Aspergillus nucleobase transporters. Biochimica et Biophysica Acta, 1442, 353–360.
   PubMedGoogle Scholar
• Ferreira, T., Brethes, D., Napias, C. and Chevallier, J., 1997, Functional analyses of mutated purine-cytosine permease from Saccharomyces cerevisia e. A possible role of the hydrophilic segment 371 377 in the active carrier conformation. Journal of Biological Chemis try, 272, 9697–9702.
   PubMed Web of Science ®Google Scholar
• Ferreira, T., Chevallier, J., Paumard, P., Napias, C. and Brethes, D., 1999, Screening of intragenic second-site suppressor of purine-cytosine permease from Saccharomyces cerevisia e. Possible role of Ser272 in the base translocation process. European Journal of Biochemistry, 260, 22–30.
   PubMedGoogle Scholar
• Gordon, S. E., Oakley, J. C., Varnum, M. D. and Zagotta, W. N., 1996, Altered ligand specificity by protonation in the ligand binding domain of cyclic nucleotide-gated channels. Biochemistry, 35, 3994–4001.
   Google Scholar
• Gorfinkiel, L., Diallinas, G. and Scazzocchio, C., 1993, Sequence and regulation of the uapA gene encoding a uric acid-xanthine permease in the fungus Aspergillus nidulans. <i>Journal of Biological Chemis try, 268, 23376–23381.
   Google Scholar
• Guimaraes, J. M., Bazan, J. F., Zlotnik, A., Wiles, M. V., Grimaldi, F. L. and McClanahan, T., 1995, A new approach to the study of the haematopoitic development in the yolk sac of embryoid bodies. Development, 121, 3335–3346.
   PubMedGoogle Scholar
• Hoffenberg, S., Nikolova, L., Pan, J. Y., Daniels, D. S., Wessling-Resnick, M., Knoll, B. J. and Dickey, B. F., 1995, Functional and structural interactions of the Rab5 D136N mutant with xanthine nucleotides. Biochemical and Biophys ical Research Commu-nica tions, 215, 441–249.
   Google Scholar
• Kang, C., Sun, N., Hontzatko, R. B. and Fromm, H. J., 1994, Replacement of Asp333 with Asn by site-directed mutagenesis changes the substrate specificity of Escherichia coli adenylo-succinate synthetase from guanosine 5 - to xanthosine 5 -triphosphate. Journal of Biological Chemistry, 269, 24046 24049.
   Google Scholar
• Kraupp, M. and Marz, R., 1995, Membrane transportof nucleobases: interaction with inhibitors. General Pharmacology, 26, 1185 1190.
   Google Scholar
• Lockington, R. A., Sealy-Lewis, H. N., Scazzocchio, C. and Davies, R. W., 1985, Cloning and characterisation of the ethanol utilisation regulon in Aspergillus nidulans. Gene, 33, 137–149.
   PubMedGoogle Scholar
• Loewen, S. K., Ng, A. M., Yao, S. Y., Cass, C. E., Baldwin, S. A. and Young, J. D., 1999, Identification of amino acid residues responsible for the pyrimidine and purine nucleoside specificities of human concentrative Na(+) nucleoside cotransporters hCNT1 and hCNT2. Journal of Biological Chemistry, 274, 24475–24484.
   PubMed Web of Science ®Google Scholar
• Rajan D. P., Huang, W., Dutta, B., Devoe, L. D., Leibach, F. H., Ganapathy, V. and Prasad, P. D., 1999, Human placental sodium-dependent vitamin C transporter (SVTC2): Molecular cloning and transport function. Biochemical Biophysical Research Communications, 262, 762–768.
   PubMed Web of Science ®Google Scholar
• Ravagnani, A., Gorfinkiel, L., Langdon, T., Diallinas, G., Adjaj, E., Demais, S., Gorton, D., Arst, H. N. Jr. and Scazzocchio, C., 1997, Subtle hydrophobic interactions between the seventh residue of the Zinc finger loop and the first base of an HGATAR sequence determine promoter-specific recognition by the Aspergillus nidulans GATA factor AreA. E uropean Molecular Biology Orga nisation Journal, 16, 3974–3986.
   Google Scholar
• Sambrook, J., Fritsch, E. F. and Maniatis, T., 1989, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory).
   Google Scholar
• Scazzocchio, C., 1994, The purine degradation pathway, genetics, biochemistry and regulation. In Genetics and Physiology of Aspergillus, S. Martinelli, and J. R. Kinghorn, eds (Amsterdam: Elsevier Science Publishers BV), pp. 221–257.
   Google Scholar
• Scazzocchio, C. and Gorton, D. J., 1977, The regulation of purine breakdown. In Genetics and Physiology of Aspergillus, J. E. Smith and J. A. Pateman, eds (NewYork: Academic Press), pp. 255–265.
   Google Scholar
• Scazzocchio, C., Sdrin, N. and Ong, G., 1982, Positive regulation in a eukaryote, a study of the uaY gene of Aspergillus nidulans: I. Characterisation of alleles, dominance and complementation studies and a fine structure map of the uaY-oxpA cluster. Genetics, 100, 185–208.
   PubMedGoogle Scholar
• Schultes, N. P., Brutnell, T. P., Allen, A., Dellaporta, S. L., Nelson, T. and Chen, C., 1996, Leaf permease1 gene of maize is required for chloroplast development. The Plant Cell, 8, 463–475.
   PubMed Web of Science ®Google Scholar
• Stoeckler, J. D., Poirot, A. F., Smith, H. B., Parks, R. E. Jr, Ealick, S. E., Takabayashi, K. and Erion, M. D., 1997 Purine nucleoside phosphorylase. 3. Reversal of purine base specificity by site-directed mutagenesis. Biochemistry, 30, 11749–11756.
   Google Scholar
• Sunahara, R. K., Beuve, A., Tesmer, J. J. G., Sprang, S. R., Garbers, D. L. and Gilman, A. G., 1998, Exchange of substrate and inhibitor specificitie s between adenyl and guanyl cyclases. Journal of Biological Chemis try, 273, 16332–16338.
   Google Scholar
• Tazebay, U. H., Sophianopoulou, V., Cubero, B., Scazzocchio C. and Diallinas, G., 1995, Post-transcriptional regulation and kinetic characterization of proline transport in germinating conidiospores of Aspergillus nidulans. FEMS Microbiology Letters, 132, 27–37.
   Google Scholar
• Tsukaguchi, H., Tokui, T., Mackenzie, B., Berger, U. V., Chen, X. Z., Wang, Y., Brubaker, R. F. and Hediger, M. A., 1999, A family of mammalian Na+-dependent L-ascorbic acid transporters. Na t-ure, 399, 70–75.
   PubMed Web of Science ®Google Scholar
• Tucker, C. L., Hurley, J. H., Miller, T. R., and Hurley, J. B., 1998, Two amino acid substitutions convert a guanyl cyclase, RetGC-1, into an adenyl cyclase. Proceedings of the National Acade my of Sciences, USA, 95, 5993–5997.
   Google Scholar
• Varnum, M. D., Black, K. D. and Zagotta, W. N., 1995, Molecular mechanism for ligand discrimination of cyclic nucleotide-gated channels. Neuron, 15, 619–625.
   PubMed Web of Science ®Google Scholar
• Wang, J. and Giacomini, K. M., 1997, Molecular determinants of substrate selectivity of Na+-dependent nucleoside transporters. Journal of Biological Chemis try, 272, 28845–28848.
   PubMed Web of Science ®Google Scholar
• Wang, J. and Giacomini, K. M., 1999, Serine 318 is essential for pyrimidine selectivity of the Na+-nucleoside transporter. Journal of Biological Chemistry, 274, 2298 2302.
   Google Scholar