GPCR-induced dissociation of G-protein subunits in early stage signal transduction

References

• Wise A, Gearing K, Rees S. Target validation of G-protein coupled receptors. Drug Discov Today 2002; 7: 235–246
   PubMed Web of Science ®Google Scholar
• Vassilatis DK, Hohmann JG, Zeng H, Li F, Ranchalis JE, Mortrud MT, Brown A, Rodriguez SS, Weller JR, Wright AC, Bergmann JE, Gaitanaris GA. The G protein-coupled receptor repertoires of human and mouse. Proc Natl Acad Sci USA 2003; 100: 4903–4908
   PubMed Web of Science ®Google Scholar
• Gales C, Rebois RV, Hogue M, Trieu P, Breit A, Hebert TE, Bouvier M. Real-time monitoring of receptor and G-protein interactions in living cells. Nat Methods 2005; 2: 177–184
   PubMed Web of Science ®Google Scholar
• Neer EJ. Heterotrimeric G proteins: organizers of transmembrane signals. Cell 1995; 80: 249–257
   PubMed Web of Science ®Google Scholar
• Hepler JR, Gilman AG. G proteins. Trends Biochem Sci 1992; 17: 383–387
   PubMed Web of Science ®Google Scholar
• Wall MA, Coleman DE, Lee E, Iniguez-Lluhi JA, Posner BA, Gilman AG, Sprang SR. The structure of the G-protein heterotrimer Giα1β1γ2. Cell 1995; 83: 1047–1058
   PubMed Web of Science ®Google Scholar
• Mixon MB, Lee E, Coleman DE, Berghuis AM, Gilman AG, Sprang SR. Tertiary and quaternary structural changes in Giα1 induced by GTP hydrolysis. Science 1995; 270: 954–960
   Google Scholar
• Lambright DG, Sondek J, Bohm A, Skiba NP, Hamm HE, Sigler PB. The 2.0 A crystal structure of a heterotrimeric G-protein. Nature 1996; 379: 311–319
   PubMed Web of Science ®Google Scholar
• Watson N, Linder ME, Druey KM, Kehrl JH, Blumer KJ. RGS family members: GTPase-activating proteins for heterotrimeric G-protein alpha-subunits. Nature 1996; 383: 172–175
   PubMedGoogle Scholar
• Druey KM, Blumer KJ, Kang VH, Kehrl JH. Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family. Nature 1996; 379: 742–746
   PubMed Web of Science ®Google Scholar
• Doupnik CA, Davidson N, Lester HA, Kofuji P. RGS proteins reconstitute the rapid gating kinetics of Gβγ-activated inwardly rectifying K+ channels. Proc Natl Acad Sci USA 1997; 94: 10461–10466
   PubMed Web of Science ®Google Scholar
• Sherwood L The plasma membrane and membrane potential. In: Sherwood L, editor. Human physiology – from cells to systems. 3rd ed. Belmont, CA: Wadsworth; 1997. pp 46–80.
   Google Scholar
• Goding JW The plasma-membrane receptors and GTP-binding proteins. In: Barritt GJ, editor. Communication within animal cells. New York: Oxford University Press; 1992. pp 53–89.
   Google Scholar
• Frank M, Thumer L, Lohse MJ, Bunemann M. G Protein activation without subunit dissociation depends on a Gαi-specific region. J Biol Chem 2005; 280: 24584–24590
   PubMed Web of Science ®Google Scholar
• Bieri C, Ernst OP, Heyse S, Hofmann KP, Vogel H. Micropatterned immobilization of a G protein-coupled receptor and direct detection of G-protein activation. Nat Biotechnol 1999; 17: 1105–1108
   PubMed Web of Science ®Google Scholar
• Simons PC, Shi M, Foutz T, Cimino DF, Lewis J, Buranda T, Lim WK, Neubig RR, McIntire WE, Garrison J, Prossnitz E, Sklar LA. Ligand–receptor–G-protein molecular assemblies on beads for mechanistic studies and screening by flow cytometry. Mol Pharmacol 2003; 64: 1227–1238
   Google Scholar
• Milligan G. Principles: extending the utility of [35S]GTPγS binding assays. Trends Pharmacol Sci 2003; 24: 87–90
   PubMed Web of Science ®Google Scholar
• Harrison C, Traynor JR. The [35S]GTPγS binding assay: Approaches and applications in pharmacology. Life Sci 2003; 74: 489–508
   PubMed Web of Science ®Google Scholar
• Massotte D. G protein-coupled receptor overexpression with the baculovirus-insect cell system: A tool for structural and functional studies. Biochim Biophys Acta 2003; 1610: 77–89
   Google Scholar
• Lim WK, Neubig RR. Selective inactivation of guanine-nucleotide-binding regulatory protein (G-protein) α and βγ subunits by urea. Biochem J 2001; 354: 337–344
   Google Scholar
• Kozasa T, Gilman AG. Purification of recombinant G proteins from Sf9 cells by hexahistidine tagging of associated subunits. Characterization of α12 and inhibition of adenylyl cyclase by αz. J Biol Chem 1995; 270: 1734–1741
   PubMedGoogle Scholar
• Wei Q, Zhou DH, Shen QX, Chen J, Chen LW, Wang TL, Pei G, Chi ZQ. Human mu-opioid receptor overexpressed in Sf9 insect cells functionally coupled to endogenous Gi/o proteins. Cell Res 2000; 10: 93–102
   Google Scholar
• Knight PJ, Grigliatti TA. Diversity of G proteins in Lepidopteran cell lines: Partial sequences of six G protein alpha subunits. Arch Insect Biochem Physiol 2004; 57: 142–150
   Google Scholar
• Uhlen S, Porter AC, Neubig RR. The novel alpha-2 adrenergic radioligand [3H]-MK912 is alpha-2C selective among human alpha-2A, alpha-2B and alpha-2C adrenoceptors. J Pharmacol Exp Ther 1994; 271: 1558–1565
   Google Scholar
• Simons PC, Biggs SM, Waller A, Foutz T, Cimino DF, Guo Q, Neubig RR, Tang WJ, Prossnitz ER, Sklar LA. Real-time analysis of ternary complex on particles: Direct evidence for partial agonism at the agonist–receptor–G protein complex assembly step of signal transduction. J Biol Chem 2004; 279: 13514–13521
   PubMed Web of Science ®Google Scholar
• Sarvazyan NA, Lim WK, Neubig RR. Fluorescence analysis of receptor–G protein interactions in cell membranes. Biochemistry 2002; 41: 12858–12867
   Google Scholar
• Waller A, Simons PC, Biggs SM, Edwards BS, Prossnitz ER, Sklar LA. Techniques: GPCR assembly, pharmacology and screening by flow cytometry. Trends Pharmacol Sci 2004; 25: 663–669
   Google Scholar
• Waller A, Simons P, Prossnitz ER, Edwards BS, Sklar LA. High throughput screening of G-protein coupled receptors via flow cytometry. Comb Chem High Throughput Screen 2003; 6: 389–397
   Google Scholar
• Bennett TA, Key TA, Gurevich VV, Neubig RR, Prossnitz ER, Sklar LA. Real-time analysis of G protein-coupled receptor reconstitution in a solubilized system. J Biol Chem 2001; 276: 22453–22460
   Google Scholar
• Sklar LA, Vilven J, Lynam E, Neldon D, Bennett TA, Prossnitz E. Solubilization and display of G protein-coupled receptors on beads for real-time fluorescence and flow cytometric analysis. BioTechniques 2000; 28: 976–975
   Google Scholar
• Bunemann M, Frank M, Lohse MJ. Gi protein activation in intact cells involves subunit rearrangement rather than dissociation. Proc Natl Acad Sci USA 2003; 100: 16077–16082
   PubMed Web of Science ®Google Scholar