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ORIGINAL ARTICLE

Identification of a γ-hexachlorocyclohexane dehydrochlorinase (LinA) variant with improved expression and solubility properties

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Pages 223-230 | Received 22 Jul 2005, Published online: 11 Jul 2009

References

  • Boltner D, Moreno-Morillas S, Ramos JL. 16S rDNA phylogeny and distribution of lin genes in novel hexachlorocyclohexane-degrading Sphingomonas strains. Environ Microbiol 2005; 7: 1329–1338
  • Dogra C, Raina V, Pal R, Suar M, Lal S, Gartemann KH, Holliger C, van der Meer JR, Lal R. Organization of lin genes and IS6100 among different strains of hexachlorocyclohexane-degrading Sphingomonas paucimobilis: evidence for horizontal gene transfer. J Bacteriol 2004; 186: 2225–2235
  • Imai R, Nagata Y, Fukuda M, Takagi M, Yano K. Molecular cloning of a Pseudomonas paucimobilis gene encoding a 17-kilodalton polypeptide that eliminates HCl molecules from gamma-hexachlorocyclohexane. J Bacteriol 1991; 173: 6811–6819
  • Johri AK, Dua M, Tuteja D, Saxena R, Saxena DM, Lal R. Genetic manipulations of microorganisms for the degradation of hexachlorocyclohexane. FEMS Microbiol Rev 1996; 19: 69–84
  • Kumari R, Subudhi S, Suar M, Dhingra G, Raina V, Dogra C, Lal S, van der Meer JR, Holliger C, Lal R. Cloning and characterization of lin genes responsible for the degradation of Hexachlorocyclohexane isomers by Sphingomonas paucimobilis strain B90. Appl Environ Microbiol 2002; 68: 6021–6028
  • Mohn WW, Mertens B, Neufeld JD, Verstraete W, de Lorenzo V. Distribution and phylogeny of hexachlorocyclohexane-degrading bacteria in soils from Spain Environ Microbiol 2006a; 8: 60–68
  • Mohn W, Garmendia J, Galvao T, Lorenzo V. Surveying biotransformations with a la carte genetic traps: translating dehydrochlorination of lindan(gamm-hexachlorocyclohexane) into lacZ-based phenotypes. Env Microbiol 2006b; 8: 546–555
  • Nagata Y, Futamura A, Miyauchi K, Takagi M. Two different types of dehalogenases, LinA and LinB, involved in gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26 are localized in the periplasmic space without molecular processing. J Bacteriol 1999a; 181: 5409–5413
  • Nagata Y, Hatta T, Imai R, Kimbara K, Fukuda M, Yano K, Takagi M. Purification and characterization of g-hexachlorocyclohexane (g-HCH) dehydrochlorinase (LinA) from Pseudomonas paucimobilis. Biosci Biotechnol Biochem 1993; 57: 1582–1583
  • Nagata Y, Miyauchi K, Takagi M. Complete analysis of genes and enzymes for gamma-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26. J Ind Microbiol Biotechnol 1999b; 23: 380–390
  • Nagata Y, Mori K, Takagi M, Murzin AG, Damborsky J. Identification of protein fold and catalytic residues of gamma-hexachlorocyclohexane dehydrochlorinase LinA. Proteins 2001; 45: 471–477
  • Neufeld JD, Mohn WW, de Lorenzo V. Composition of microbial communities in hexachlorocyclohexane (HCH) contaminated soils from Spain revealed with a habitat-specific microarray. Environ Microbiol 2005; 8: 126–140
  • Phillips TM, Seech AG, Lee H, Trevors JT. Colorimetric assay for Lindane dechlorination by bacteria. J Microbiol Methods 2001; 47: 181–188
  • Sahu SK, Patnaik KK, Sharmila M, Sethunathan N. Degradation of α, β, γ-hexachlorocyclohexane by a soil bacterium under aerobic conditions. Appl Environ Microbiol 1990; 56: 3620–3622
  • Strong LC, McTavish H, Sadowsky MJ, Wackett LP. Field-scale remediation of atrazine-contaminated soil using recombinant Escherichia coli expressing atrazine chlorohydrolase. Environ Microbiol 2000; 2: 91–98
  • Thomas JC, Berger F, Jacquier M, Bernillon D, Baud-Grasset F, Truffaut N, Normand P, Vogel TM, Simonet P. Isolation and characterization of a novel gamma-hexachlorocyclohexane-degrading bacterium. J Bacteriol 1996; 178: 6049–6055
  • Thomas JD, Daniel RA, Errington J, Robinson C. Export of active green fluorescent protein to the periplasm by the twin-arginine translocase (Tat) pathway in Escherichia coli. Mol Microbiol 2001; 39: 47–53

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