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Biotechnic & Histochemistry Volume 95, 2020 - Issue 1
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Articles

In tube immunocytochemistry for fluorescence-activated cell sorting that prevents RNA degradation in sorted cells

H. FukanoDivision of Health Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, JapanView further author information
,
T. TakanoDepartment of Metabolic Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, JapanCorrespondence[email protected]
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,
Y. FujimotoDivision of Health Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, JapanView further author information
,
R. NakataniDepartment of Metabolic Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, JapanView further author information
,
M. WatanabeDivision of Health Sciences, Osaka University Graduate School of Medicine, Suita, Osaka, JapanORCID Iconhttps://orcid.org/0000-0001-5971-9296View further author information
ORCID Icon,
Y. HidakaDepartment of Laboratory Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, JapanView further author information
&
I. ShimomuraDepartment of Metabolic Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, JapanView further author information
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Pages 1-7 | Published online: 19 Aug 2019

References

  • Cleland WW. 1964. Dithiothreitol, a new protective reagent for SH groups. Biochemistry. 3:480–482.
  • Date A, Maeda T, Watanabe M, Hidaka Y, Iwatani Y, Takano T. 2014. An improved protocol for mRNA quantification after fluorescence-activated cell sorting with an increased signal to noise ratio in flow cytometry. Mol Biotechnol. 56:591–598. doi:10.1007/s12033-014-9733-5.
  • Julia A, Barcelo M, Erra A, Palacio C, Marsal S. 2009. Identification of candidate genes for rituximab response in rheumatoid arthritis patients by microarray expression profiling in blood cells. Pharmacogenomics. 10:1697–1708. doi:10.2217/pgs.09.99.
  • Krutzik PO, Nolan GP. 2006. Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling. Nat Methods. 3:361–368. doi:10.1038/nmeth872.
  • Lin RY. 2011. Thyroid cancer stem cells. Nat Rev Endocrinol. 7:609–616. doi:10.1038/nrendo.2011.127.
  • Maeda T, Date A, Watanabe M, Hidaka Y, Iwatani Y, Takano T. 2016. Optimization of recovery and analysis of RNA in sorted cells in mRNA quantification after fluorescence-activated cell sorting. Ann Clin Lab Sci. 46:571–577.
  • Maruo R, Yamada H, Watanabe M, Hidaka Y, Iwatani Y, Takano T. 2011. mRNA quantification after fluorescence activated cell sorting using locked nucleic acid probes. Mol Biotechnol. 49:42–47. doi:10.1007/s12033-011-9375-9.
  • Matsumoto C, Ito M, Yamada H, Yoshida H, Watanabe M, Hidaka Y, Iwatani Y, Miyauch A, Takano T. 2013. Preparation of thyroid follicular cells for mRNA quantification after fluorescence-activated cell sorting. Scand J Clin Lab Invest. 73:245–252. doi:10.3109/00365513.2013.769624.
  • Shimasue A, Yamakawa N, Watanabe M, Hidaka Y, Iwatani Y, Takano T. 2015. Expression analysis of stemness genes in a rat thyroid cell line FRTL5. Exp Clin Endocr Diabetes. 123:48–54.
  • Takano T. 2014. Fetal cell carcinogenesis of the thyroid: a modified theory based on recent evidence. Endocr J. 61:311–320.
  • Yamada H, Maruo R, Watanabe M, Hidaka Y, Iwatani Y, Takano T. 2010a. Messenger RNA quantification after fluorescence activated cell sorting using intracellular antigens. Biochem Biophys Res Commun. 397:425–428. doi:10.1016/j.bbrc.2010.05.112.
  • Yamada H, Maruo R, Watanabe M, Hidaka Y, Iwatani Y, Takano T. 2010b. Messenger RNA quantification after fluorescence-activated cell sorting using in situ hybridization. Cytometry A. 77:1032–1037. doi:10.1002/cyto.a.20973.
  • Yamada H, Yamakawa N, Watanabe M, Hidaka Y, Iwatani Y, Takano T. 2012. Prolonged hybridization with a cRNA probe improves the signal to noise ratio for in-tube in situ hybridization for quantification of mRNA after fluorescence-activated cell sorting. Biotech Histochem. 87:366–371. doi:10.3109/10520295.2012.672650.
  • Yoshizaki T, Inaji M, Kouike H, Shimazaki T, Sawamoto K, Ando K, Date I, Kobayashi K, Suhara T, Uchiyama Y, Okano H. 2004. Isolation and transplantation of dopaminergic neurons generated from mouse embryonic stem cells. Neurosci Lett. 363:33–37. doi:10.1016/j.neulet.2004.03.074.
  • Zuba-Surma EK, Kucia M, Ratajczak J, Ratajczak MZ. 2009. “Small stem cells” in adult tissues: very small embryonic-like stem cells stand up!. Cytometry A. 75:4–13. doi:10.1002/cyto.a.20665.

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