42
Views
21
CrossRef citations to date
0
Altmetric
Research Article

Use of the random amplif|ed polymorphic DNA (RAPD) assay for the detection of DNA damage and mutations: possible implications of confounding factors

, , &
Pages 94-101 | Published online: 20 Oct 2008

  • ASTM 1975, Method for acute toxicity tests with fish, macro-invertebrates and amphibians. EPA-660/ 3-75-009, American Society for Testing Materials, Springfield, USA, 360-379.
  • ATIENZAR, F. A. 2000, Development of the random amplified polymorphic DNA (RAPD) technique to measure the effects of genotoxins in aquatic organisms. Ph D thesis, University of Plymouth, UK.
  • ATIENZAR, F. A., CONRADI, M., EVENDEN, A. J., JHA, A. N. and DEPLEDGE, M. H. 1999, Qualitative assessment of genotoxicity using random amplified polymorphic DNA: comparison of genomic template stability with key fitness parameters in Daphnia magna exposed to benzo[a]pyrene. Environmental Toxicology and Chemistry, 18, 2275-2282.
  • ATIENZAR, F. A., CORDI, B., DONKIN, M. E., EVENDEN, A. J., JHA, A. N. and DEPLEDGE, M. H. 2000a, Comparison of ultraviolet-induced genotoxicity detected by random amplified polymorphic DNA with chlorophyll fluorescence and growth in a marine macroalgae, Polmaria pabnata. Aquatic Toxicology, 50, 1-12.
  • ATIENZAR, F. A., EVENDEN, A. J., JHA, A. N., SAVVA, D. and DEPLEDGE, M. H. 2000b, Optimized RAPD analysis generates high-quality genomic DNA profiles at high annealing temperature. BioTechniques, 28, 52-54. A corrigendum was published in 2000, 26(6), 1195: dNTP concentration in Table 1 should be 0.33 mM instead of 0.33µM.
  • ATIENZAR, F. A., CHEUNG, V. V., JHA, A. N. and DEPLEDGE, M. H. 2001, Fitness parameters and DNA effects are sensitive indicators of copper-induced toxicity in Daphnia magna. Toxicological Sciences, 19, 241-250.
  • AYLIFFE, M. A., LAWRENCE, G. J., ELLIS, J. G. and PRYOR, A. J. 1994, Heteroduplex molecules formed between allelic sequences cause nonparental RAPD bands. Nucleic Acids Research, 22, 1632-1636.
  • BECERRIL, C., FERRERO, M., SANZ, F. and CASTANO, A. 1999, Detection of mitomycin C-induced genetic damage in fish cells by use of RAPD. Mut agenesis, 14, 449-456.
  • BIELAWSKI, J. P., NOACK, K. and PUMO, D. E. 1995, Reproducible amplification of RAPD markers from vertebrate DNA. BioTechniques, 18, 856-860.
  • BOWDITCH, B. M., ALBRIGHT, D. G., WILLIAMS, J. G. K. and BRAUN, M. J. 1993, Use of randomly amplified polymorphic DNA markers in comparative genome studies. Methods in Enzymology, 224, 294-309.
  • HANAWALT, P. C. 1998, Genomic instability: environmental invasion and the enemies within. Mutation Research, 400, 117-125.
  • KHANDKA, D. K., TUNA, M., TAL, M., NEJIDAT, A. and GOUNGOLDHIRSH, A. 1997, Variability in the pattern of random amplified polymorphic DNA. Electrophoresis, 18, 2852-2856.
  • KOHWI, Y. and KOHWI-SHIGEMATSU, T. 1991, Altered gene expression correlates with DNA structure. Genes and Development, 5, 2547-2554.
  • LOPEZ, A., XAMENA, N., CABRE, O., CREUS, A., MARCOS, R. and VELAZQUEZ, A. 1999, Analysis of genomic damage in the mutagen sensitive mus-201 mutant of Drosophila melanogaster by arbitrarily primed PCR (AP-PCR) fingerprinting. Mutation Research, 435, 63-75.
  • SHIMADA, A. and SHIMA, A. 1998, Combination of genomic DNA fingerprinting into the medaka specific locus test system for studying environmental germ line mutagenesis. Mutation Research, 399, 149-165.
  • WELSH, J. and McCCLELLAND, M. 1990, Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research, 18, 7213-7218.
  • WELSH, J., RALPH, D. and McCLELLAND, M. 1995, DNA and RNA fingerprinting using arbitrary primed PCR. In PCR Strategies, edited by M. A. Innis, D. H. Gelfand and J. J. Sninsky (San Diego: Academic Press), pp. 249-276.
  • WILLIAMS, J. G. K., KUBELIK, A. R., LIVAK, K. J., RAFALSKI, J. A. and TINGEY, S. V. 1990, DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18, 6531-6535.
  • WOLFFE, A. P. and HAVES, J. J. 1999, Chromatin disruption and modification. Nucleic Acids Research, 27, 711-720.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.