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Research Article

Development of cytochrome P450 as a biomarker of organic pollution in Mytilus sp.: field studies in United Kingdom ('Sea Empress' oil spill) and the Mediterranean Sea

Pages 425-441 | Published online: 29 Sep 2008

References

  • ADE, P, BANCHELLI SOLDAINI, M. G., CASTELLI, M. G., CHIERSARA, E., CLEMENTI, E, FANELLI, R, FUNARI, E., INGESTI, G., MARABINI, A, ORONESU, M., PALMERO, S., PIRISINO, R, RAMUNDO ORLANDO, A, SILO, V, VIARENGO, A and VITTOZZI, L. 1982, Comparative biochemical and morphological characterizations of microsomal preparations from rat, quail, trout, mussel and Daphnia magna. In Cytochrome P-450. Biochemistry, biophysics and environmental implications, E. Hietanen, M. Laitinen and 0. Hfinninen, eds (Holland: Elsevier) pp. 387–390.
  • BAUMARD, P 1997, Biogeochimie des composes armoatiques dans l'environment marin. PhD dissertation, University of Bordeaux I, France.
  • BROWN, D. J., CLARK, G. C and VAN BENEDEN, R. J. 1998, A new cytochrome P450 (CYP30) family identified in the clam Mercenaria mercenaria. Comparative Biochemistry and Physiology, 121C, 351–360.
  • BUCHELI, T. D. and PENT, K. 1995, Induction of cytochrome P450 as a biomarker for environmental contamination in aquatic ecosystems. Critical Reviews in Environmental Science and Technology, 25, 201–268.
  • BUTTY, J. S. and HOLDWAY, D. A 1997, Assessment of the octopus Octopus pallidus, as a potential bioindicator species of xenobiotic challenge: baseline studies. Marine Pollution Bulletin, 34, 564–570.
  • CANOVA, S., DEGAN, P, PETERS, L. D., LIVINGSTONE, D. R., VOLTAN, R. and VENTER, P 1988, Tissue dose, DNA adducts, oxidative DNA damage and CYP1A-immunopositive proteins in mussels exposed to waterborne benzo[a]pyrene. Mutation Research, 399, 17–30.
  • CHEAH, D. M. Y, WRIGHT, P. E A, HOLDWAY, D. A and AHOKAS, J. T. 1995, Octopus pallidus cytochrome P-450: characterization and induction studies with 0-naphthoflavone and aroclor 1254. Aquatic Toxicology, 33,102–214.
  • DEHNEN, W., TOMINGAS, R and Roos, J. 1973, A modified method for assay of benzo[a]pyrene hydroxylase. Analytical Biochemistry, 53,378–383.
  • DEPLEDGE, M. H. and Foss', M. C. 1994, The role of biomarkers in environmental assessment (2). Invertebrates. Ecotoxicology, 3,161–172.
  • DYRYNDA, E A, LAW, R J., DYRYNDA, E E. J., KELLY, C. A, PIPE, R K., GRAHAM, K. L. and RATCLIFFE, N. A 1997, Modulations in cell-mediated immunity of Mytilus edulis following the 'Sea Empress' oil spill. Journal of the Marine Biological Association of the UK, 77,281–284.
  • ERTL, R E and WINSTON, G. W. 1998, A review of the mixed function oxidase system in amphibians and reptiles. Comparative Biochemistry and Physiology, 121C, 85–105.
  • FALCHK, E H. J. and HERRON, K. D. 1998, Constitutive CYP1A is not expressed in the invertebrate Octopus pallidus Proceedings of Australian Society of Clinical and Experimental Pharmacologists and Toxicologists, 30th Novernber-4th December, Canberra, Australia, p. 25.
  • GOKSOVR, A 1995, Use of cytochrome P4501A (CYP1A) in fish as a biomarker of aquatic pollution. Archives of Toxicology, Supplement 17,80–95.
  • HERRON, K. and FALCKH, E H. J. 1998, CYP1A-like sequences isolated from the invertebrate, Mytilus edulis. In Proceedings of Australian Society of Clinical and Experimental Pharmacologists and Toxicologist 4 30th November-4th December, Canberra, Australia, p. 24.
  • HUGGETT, R J., KIMERLE, R A, MEHRLE, E M. JR and BERGMAN, H. L. (eds) (1992), Biomarkers. Biochemical, Physiological and Histological Markers of Anthropogenic Stress (Boca Raton, Florida: Lewis Publishers).
  • HUGHES, W. W. 1996, Essentials of Environmental Toxicology (Washington: Taylor and Francis).
  • KIRCHIN, M. A 1988, Cytochrome P450 of the common mussel, Mytilus edulis L.: partial purification and characterization. PhD dissertation, University of Surrey, UK.
  • KIRCHIN, M. A, WISEMAN, A and LIVINGSTONE, D. R. 1992, Seasonal and sex variation in the mixed function oxygenase system of digestive gland microsomes of the common mussel, Mytilus edulis L. Comparative Biochemistry and Physiology, 101C, 81–91.
  • KLOEPPER-SAMS, E J. and STEGEMAN, J. J. 1989, The temporal relationships between P450E protein content, catalytic activity ad mRNA levels in the teleost Fundulusheteroditus following treatment with fl-naphthaflavone. Archives of Biochemistry and Biophysics, 268,525–535.
  • LAEMMLI, U. K. 1970, Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature, 227,680–685.
  • LEMMRE, E and LIVINGSTONE, D. R. 1993, Pro-oxidant/antioxidant processes and organic xenobiotic interactions in marine organisms, in particular the flounder Platicthys flesus and the mussel Mytilus edulis. Trends in Comparative Biochemistry and Physiology, 1, 1119–1149.
  • LEMAIR EE, DEN BESTEN, E J., O'HARA, S. C. M. and LIVINGSTONE, D. R. 1993, Comparative metabolism of benzo[a]pyrene by digestive gland microsomes of the shore crab Carcinus maenus L. and the common mussel Mytilus edulis L. Polycydic Aromatic Compounds, 3 (suppl), 1133–1140.
  • LIVINGSTONE, D. R 1985, Responses of the detexication/toxication enzyme systems of molluscs to organic pollutants and xenobiotics. Marine Pollution Bulletin, 16, 158–164.
  • LIVINGSTONE, D. R 1987, Seasonal responses to diesel oil and subsequent recovery of the cytochrome P450 monooxygenase system in the common mussel, Mytilus edulis L., and the periwinkle Littorina littorina L. Science of the Total Environment, 65, 209–235.
  • LIVINGSTONE, D. R 1988, Responses of microsomal NADPH-cytochrome c reductase activity and cytochrome P-450 in digestive glands of Mytilus edulis and Littorina littorea to environmental exposure pollutants. Marine Ecology Progress Series, 46, 37–43.
  • LIVINGSTONE, D. R 1993, Biotechnology and pollution monitoring: use of molecular biomarkers in the aquatic environment. Journal of Chemical Technology and Biotechnology, 57, 195–211.
  • LIVINGSTONE, D. R 1991a, Organic xenobiotic metabolism in marine invertebrates. Advances in Comparative and Environmental Physiology, Vol. 7, R. Gilles, ed. (Berlin: Springer-Verlag), pp. 45–185.
  • LIVINGSTONE, D. R 1991b, Towards a specific index of impact by organic pollution for marine invertebrates. Comparative Biochemistry and Physiology, 100C, 151–155.
  • LIVINGSTONE, D. R 1996, Cytochrome P450 in pollution monitoring. Use of cytochrome P4501A (CYP1A) as a biomarker of organic pollution in aquatic and other organisms. In Environmental Xenobiotics, M. Richardson, ed. (London: Taylor &Francis), pp. 143–160.
  • LIVINGSTONE, D. R and FARRAR, S. V. 1984, Tissue and subcellular distribution of enzyme activities of mixed-function oxygenase and benzo[a]pyrene metabolism in the common mussel Mytilus edulis L. Science of the Total Environment, 39, 209–235.
  • LIVINGSTONE, D. R and GOLDFARB, E S. 1998, Aquatic environmental biomonitoring: use of cytochrome P450 1A and other molecular biomarkers in fish and mussels. In Biotechnology Research Series, Vol. 6 Environmental Biornonitoring The Biotechnology Ecotoxicology Interface, J. Lynch and A Wiseman, eds (Cambridge: Cambridge University Press), pp. 101–129.
  • LIVINGSTONE, D. R and PIPE, R K. 1992, Mussels and environmental contaminants: molecular and cellular aspects. In The Mussel Mytilus: Ecology, Physiology, Genetics and Culture, Developments in Aquaculture and Fisheries Science, Vol. 25, E. Gosling ed. (Amsterdam: Elsevier Science Publishers), pp. 425–464.
  • LIVINGSTONE, D. R, FARRAR, S. V, Foss', C., KIRCHIN, M. A and MOORE, M. N. 1988, Responses of the digestive gland cytochrome P450 monoxygenase system of the common mussel (Mytilus edulis) to 3-methylcholanthrene and sodium phenobarbital. Marine Environmental Research, 24, 118–119.
  • LIVINGSTONE, D. R, KIRCHIN, M. A and WISEMAN, A. 1989, Cytochrome P450 and oxidative metabolism in molluscs. Xenobiotica, 19, 1041–1062.
  • LIVINGSTONE, D. R, LEMAIRE, P, MATTEWS, A, PETERS, L. D., PORTE, C., FITZPATRICK, P J., FORLIN, L., NASCI, C, FOSSATO, V, WOOTTON, AN. and GOLDFARB, P S. 1995, Assessment of the impact of organic pollutants on goby (Zosterisessor ophiocephalus) and mussel (Mytilus galloprovincialis) from the Venice Lagoon, Italy: biochemical studies. Marine Environmental Research, 39, 235–240.
  • LIVINGSTONE, D. R, NASCI, C., SOLE, M., DA Ros, L., O'HARA, S. C. M., PETERS, L. D., FOSSATO, V, WOOTTON, A N. and GOLDFARB, P S. 1997, Apparent induction of a cytochrome P450 with immunochemical similarities to CYP1A in digestive gland of the common mussel (Mytilus galloprovincialis L.) with exposure to 2,2',3,4,4'5'-hexachlorobiphenyl and Arochlor 1254. Aquatic Toxicology, 38,205–224.
  • LIVINGSTONE, D. R, CHIPMAN, J. K., LOWE, D. M., MINIER, C., MICTHELMORE, C. L., MOORE, M. N., PETERS, L. D. and PIPE, R. K. 1998, Development of biomarkers to detect the effects of organic pollution on aquatic invertebrates: recent molecular, genotoxic, cellular and immunological studies on the common mussel (Mytilus edulis L.) and other mytilids. International Journal of Environmental Pollution (In press).
  • LOWRY, O. H., ROSEBROUGH, N. J., FAR, A L. and RANDALL, R. J. 1951, Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193, 266–275.
  • MCCARTHY, J. F. and SHUGART, L. R. 1990, Biornarkers of Environmental Contamination (Boca Raton, Florida: Lewis Publishers).
  • MICHEL, X., CASSAND, P M., RIBERA, D. G. and NARBONNE, J. F. 1992, Metabolism and mutagenic activation of benzo[a]pyrene by subcellular fraction from mussel (Mytilus galloprovincialis) digestive gland and sea bass (Dicentrarchus labrax) liver. Cornparative Biochemistry and Physiology, 103C, 43–55.
  • MICHEL, X. R, SUTEAU, P, ROBERTSON, L. W. and NARBONNE, J. F. 1993, Effects of benzo[a]pyrene, 3,3',4,4'-tetrachlorobiphenyl and 2,2',4,4'5,5'-hexachlorobiphenyl on the xenobiotic-metabolizing enzymes in the mussel (Mytilus galloprovincialis). Aquatic Toxicology, 27, 335–344.
  • MICHEL, X., SALAUN, J. P, GALGANI, F. and NARBONNE, J. F. 1994, Benzo(a)pyrene hydroxylase activity in the marine mussel Mytilus galloprovincialis: a potential marker of contamination by polycyclic aromatic hydrocarbon-type compounds. Marine Environmental Research, 38, 257–273.
  • MOORE, M. N., LIVINGSTONE, D. R. and WIDDOWS, J. 1989, Hydrocarbons in marine molluscs: biological effects and ecological consequences. In Metabolism of Polycydic Aromatic Hydrocarbons in the Marine Environment, U. Varanasi ed. (Florida, USA: CRC Press), pp. 291–328.
  • NARBONNE, J. E, GARRIGUES, P, RIBERA, D., RAOUX, C., MATHIEU, A, LEMAIRE, P, SALAUN, J. P and LAFAURIE, M. 1991, Mixed-function oxygenases enzymes as tools for pollution monitoring: field studies on the French coast of the Mediterranean Sea. Comparative Biochemistry and Physiology, 100C, 37–42.
  • NELSON, D. R, KOYMANS, L., KAMATAKI, T., STEGEMAN, J. J., FEYEREISEN, R., WAXMAN, D. J., WATERMAN, M. R, GOTOH, 0., COON, M. J., ESTABROOK, R. W., GUNSLUS, I. C. and NEBERT, D. W. 1996, P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Phannacogenetics, 6, 1–42.
  • PETERS, L. D. and LIVINGSTONE, D. R. 1995, Studies on cytochrome P4501A1 in early and adult life stages of turbot (S cophthalmusmcvcimus L.). Marine Environmental Research, 39, 5–9.
  • PETERS, L. D. and LIVINGSTONE, D. R. 1998, Induction of molluscan cytochrome P450 monoxygenase system as a biomarker of organic pollution in environmental monitoring. In Biomarkers in Marine Ecosystems: A Practical Approach, P Garrigues, C. H. Walker and H. Barth,eds (Holland: Elsevier) On press).
  • PETERS, L. D., MORSE, H. R, WATERS, R. and LIVINGSTONE, D. R. 1997, Responses of hepatic cytochrome P450 1A and formation of DNA-adducts in juveniles of turbot (S cophthalmus maximus L.) exposed to water-borne benzo[a]. Aquatic Toxicology, 38, 67–82.
  • PETERS, L. D., NASCI, C. and LIVINGSTONE, D. R. 1998a, Variation in levels of cytochrome P450 1A, 2B, 2E, 3A and 4A-immunopositive proteins in digestive gland of indigenous and transplanted mussel, Mytilus galloprovincialis in Venice Lagoon, Italy. Marine Environmental Research, 46, 295–299.
  • PETERS, L. D., NASCI, C and LIVINGSTONE, D. R. 1998b, Immunochemical investigations of cytochrome P450 forms/epitopes (CYP1A, 2B, 2E, 3A & 4A) in digestive gland of Mytilus sp. Comparative Biochemistry and Physiology, 121C, 361–369.
  • PORTE, C., LEMAIRE, P., PETERS, L. D. and LIVINGSTONE, D. R. 1995, Partial purification and properties of cytochrome P450 from digestive gland microsomes of the common mussel, Mytilus edulis L. Marine Environmental Research, 39,27–31.
  • QUALLS, C. W. Jr, LUBET, R A, LOCHMILLER, R. L., ELANGBAM, C. S., LISH, J. W. and Nims, R W. 1998, Cytochrome P450 induction in feral cricetid rodents: a review of field and laboratory investigations. Comparative Biochemistry and Physiology, 121C, 55–63.
  • RAND, G. (ed.) 1995, Fundamentals of Aquatic Toxicology. Effects, Environmental Fate, and Risk Assessment (London: Taylor and Francis Ltd).
  • SCHLENK, D. 1996, The role of biomarkers in risk assessment. Human and Ecological Risk Assessment, 2, 251–256.
  • SEEC, 1996, Sea Empress Environmental Evaluation Committee, Initial Report, July 1996.
  • SNYDER, M. J. 1998, Cytochrome P450 enzymes belonging to the CYP4 family from marine invertebrates. Biochemical and Biophysical Research Communications, 249, 187–190.
  • SOLE, M., PORTE, C and ALBAIGES, J. 1995a, The use of biomarkers for assessing the effects of organic pollution in mussels. Science of the Total Environment, 159, 147–153.
  • SOLE, M., PORTE, C and ALBAIGES, J. 1995b, Seasonal variation in the mixed-function oxygenase system and antioxidant enzymes of the mussel Mytilus gallop rovincialis. Environment Toxicology and Chemistry, 14, 157–164.
  • SOLE, M., PORTE, C, BIOSCA, X., MITCHELMORE, C. L., CHIPMAN, J. K., LIVINGSTONE, D. R and ALBAIGES, J. 1996, Effects of the 'Aegean Sea' oil spill on biotransformation enzymes, oxidative stress and DNA-adducts in digestive gland of the mussel (Mytilus edulis L.). Comparative Biochemistry and Physiology, 113C, 257–265.
  • SOLE, M., PETERS, L. D., MAGNUSSON, K., SJOLIN, A, GRANMO, A. and LIVINGSTONE, D. R 1998, Responses of the cytochrome P450-dependent monooxygenase and other protective enzyme systems in digestive gland of transplanted common mussel (Mytilus edulis L.) to organic contaminants in the Skagerrak and Kattegat. Biornarkers, 3, 49–62.
  • STEGEMAN, J. J. 1985, Benzo[a]pyrene oxidation and microsomal enzyme activity in the mussel (Mytilus edulis) and other bivalve mollusc species from the Western North Atlantic. Marine Biology, 89, 21–30.
  • STEGEMAN, J. J. 1992, Nomenclature for hydrocarbon-inducible cytochrome P450 in fish. Marine Environmental Research, 34, 133–138.
  • STEGEMAN, J. J. and HAHN, M. E. 1994, Biochemistry and molecular biology of monooxygenases: current perspectives on forms, functions, and regulation of cytochrome P450 in aquatic species. In Aquatic Toxicology. Molecular, Biochemical,, and Cellular Perspectives, D. C. Malins and G. K. Ostrander, eds (Boca Raton, Florida: Lewis Publishers), pp. 87-206.
  • TEUNISSEN, Y, GERAERTS, W. P. M., HEERIKHUIZEN, H. VAN, PLANTA, R. J. and JOOSSE, J. 1992, Molecular cloning of a cDNA encoding a member of a novel cytochrome P450 family in the mollusc Lymnaea stagnalis. Journal of Biochemistry, 112,.249–252.
  • TOWBIN, H., STAEHELIN, T. and GORDON, J. 1979, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, 76,4350–4354.
  • WALKER, C. H. 1996, Principles of Ecotoxicology (London: Taylor and Francis).
  • WALKER, C. H. 1998, Avian forms of cytochrome P450. Comparative Biochemistry and Physiology, 121C, 65–72.
  • WALKER, C. H. and LIVINGSTONE, D. R. 1992, Persistent Pollutants in Marine Ecosystems (Oxford, UK: Pergamon Press).
  • WOOTTON, A N. 1995, Cytochrome P450 gene expression and modulation in mussels Mytilus sp. PhD dissertation, University of Surrey, UK.
  • WOOTTON, A N., HERRRING, C., SPRY, J. A, WISEMAN, A, LIVINGSTONE, D. R. and GOLDFARB, P. S. 1995, Evidence for the existence of cytochrome P450 gene families (CYP1A, 3A, 4A, 11A) and modulation of gene expression (CYP1A) in the mussel Mytilus sp. Marine Environment Research, 39, 21–26.
  • WOOTTON, A N., GOLDFARB, P. S., LEMAIRE, P., O'HARA, S. C. M. and LIVINGSTONE, D. R 1996, Characterisation of the presence and seasonal variation of a CYP1A-like enzyme in digestive gland of the common mussel, Mytilus edulis Marine Environmental Research, 42, 297–301.

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