Assessment of DNA damages in lymphocytes of agricultural workers exposed to pesticides by comet assay in a cross-sectional study

References

• Al-Amrah, H.J., et al., 2014. Genotoxicity of waterpipe smoke in buccal cells and peripheral blood leukocytes as determined by comet assay. Inhalation toxicology, 26 (14), 891–896.
   PubMed Web of Science ®Google Scholar
• Albertini, R.J., et al., 2000. IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans: international Programme of Chemical Safety. Mutation research, 463, 111–172.
   PubMed Web of Science ®Google Scholar
• Ali, T., et al., 2017. Pesticide genotoxicity in cotton picking women in Pakistan evaluated using comet assay. Drug and chemical toxicology, 19, 1–8.
   Google Scholar
• Alves, J.S., et al., 2016. Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides. Annals of the Brazilian academy of sciences, 88 (1), 349–360.
   PubMed Web of Science ®Google Scholar
• Anderson, D., et al., 2013. The comet assay in human biomonitoring. Methods in molecular biology, 1044, 347–362.
   PubMedGoogle Scholar
• Araldi, R.P., et al., 2015. Using the comet and micronucleus assays for genotoxicity studies: a review. Biomedicine and pharmacotherapy, 72, 74–82.
   PubMed Web of Science ®Google Scholar
• Astley, S., et al., 1999. Vitamin E supplementation and oxidative damage to DNA and plasma LDL in type 1 diabetes. Diabetes care, 22, 1626–1631.
   PubMed Web of Science ®Google Scholar
• Azqueta, A., et al., 2014. Comet assay to measure DNA repair: approach and applications. Frontiers in Genetics, 5, 288–288.
   PubMed Web of Science ®Google Scholar
• Barnett, Y.A., and Barnett, C.R., 1998. DNA damage and mutation: contributors to the age-related alterations in T cell-mediated immune responses? Mechanisms of ageing and development, 102, 165–175.
   PubMed Web of Science ®Google Scholar
• Bausinger, J., and Speit, G., 2016. The impact of lymphocyte isolation on induced DNA damage in human blood samples measured by the comet assay. Mutagenesis, 31 (5), 567–572.
   PubMed Web of Science ®Google Scholar
• Benedetti, D., et al., 2013. Genetic damage in soybean workers exposed to pesticides: evaluation with the comet and buccal micronucleus cytome assays. Mutation research, 752 (1–2), 28–33.
   PubMed Web of Science ®Google Scholar
• Betti, C., et al., 1994. Microgel electrophoresis assay (comet test) and SCE analysis in human. Mutation research, 307, 323–333.
   PubMed Web of Science ®Google Scholar
• Bonassi, S., et al., 1995. Influence of sex on cytogenetic end point: evidence from a large human sample and review of the literature. Cancer epidemiology, biomarkers & prevention, 4, 671–679.
   PubMed Web of Science ®Google Scholar
• Breslow, N.E., and Day, N.E., 1980. Adaptation of the logistic model to case-control studies. In: Statistical methods in cancer research: volume 1. The analysis of case-control studies. Lyon: IARC Scientific Publications, International Agency for Research on Cancer. ISBN: 92 832 0132 9.
   Google Scholar
• Burlinson, B., et al., 2007. Fourth International Workgroup on Genotoxicity testing: results of the in vivo comet assay workgroup. Mutation research, 627, 31–35.
   PubMed Web of Science ®Google Scholar
• Burstyn, I., and De Roos, A.J., 2016. Visualizing the heterogeneity of effects in the analysis of associations of multiple myeloma with glyphosate use. Comments on Sorahan, T. Multiple Myeloma and Glyphosate Use: a re-analysis of US Agricultural Health Study (AHS) Data. International journal of environmental research and public health, 12, 1548–1559.
   Google Scholar
• Calviello, G., et al., 2006. DNA damage and apoptosis induction by the pesticide Mancozeb in rat cells: involvement of the oxidative mechanism. Toxicology and applied pharmacology, 211 (2), 87–96.
   PubMed Web of Science ®Google Scholar
• Carbajal-López, Y., et al., 2016. Biomonitoring of agricultural workers exposed to pesticide mixtures in Guerrero state, Mexico, with comet assay and micronucleus test. Environmental science and pollution research, 23, 2513–2520.
   PubMed Web of Science ®Google Scholar
• Chen, J., et al., 2007. DNA damage, cellular senescence and organismal ageing: causal or correlative? Nucleic acids research, 35, 7417–7428.
   PubMed Web of Science ®Google Scholar
• Collins, A.R., et al., 1997. The comet assay: what can it really tell us? Mutation research, 375, 183–193.
   PubMed Web of Science ®Google Scholar
• Collins, A.R., et al., 2001. Interindividual differences in repair of DNA base oxidation, measured in vitro with the comet assay. Mutagenesis, 16, 297–301.
   PubMed Web of Science ®Google Scholar
• Collins, A.R., 2004. The comet assay for DNA damage and repair: principles, applications, and limitations. Molecular biotechnology, 26, 249–261.
   PubMed Web of Science ®Google Scholar
• Collins, A.R., 2014a. Measuring oxidative damage to DNA and its repair with the comet assay. Biochimica Et Biophysica Acta, 1840 (2), 794–800.
   PubMed Web of Science ®Google Scholar
• Collins, A., et al., 2014b. The comet assay as a tool for human biomonitoring studies: the ComNet project. Mutation research/reviews in mutation research, 759, 27–39.
   PubMed Web of Science ®Google Scholar
• Da Silva, F.R., et al., 2012. Genotoxic biomonitoring of tobacco farmers: biomarkers of exposure, of early biological effects and of susceptibility. Journal of hazardous materials, 225–226, 81–90.
   PubMed Web of Science ®Google Scholar
• Da Silva, F.R., et al., 2014. Genotoxic assessment in tobacco farmers at different crop times. The science of the total environment, 490, 334–341.
   PubMed Web of Science ®Google Scholar
• De Roos, A.J., et al., 2005. Cancer incidence among glyphosate-exposed pesticide applicators in the Agricultural Health Study. Environmental health perspectives, 113 (1), 49–54.
   PubMed Web of Science ®Google Scholar
• Dosemeci, M., et al., 2002. A quantitative approach for estimating exposure to pesticides in the agricultural health study. The annals of occupational hygiene, 46 (2), 245–260.
   PubMed Web of Science ®Google Scholar
• Duthie, S.J., et al., 1996. Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes. Cancer research, 56, 1291–1295.
   PubMed Web of Science ®Google Scholar
• Eastman, A., and Barry, M.A., 1992. The origins of DNA breaks: a consequence of DNA damage, DNA repair, or apoptosis? Cancer investigation, 10, 229–240.
   PubMed Web of Science ®Google Scholar
• Frenzilli, G., et al., 1998. Comet assay on children’s leukocytes 8 years after the Chernobyl disaster. Mutation research, 415, 151–158.
   PubMed Web of Science ®Google Scholar
• George, J., and Shukla, Y., 2013. Early changes in proteome levels upon acute deltamethrin exposure in mammalian skin system associated with its neoplastic transformation potential. The journal of toxicological sciences, 38 (4), 629–642.
   PubMed Web of Science ®Google Scholar
• Geret, F., et al., 2013. Effects of low-dose exposure to pesticide mixture on physiological responses of the Pacific oyster, Crassostrea gigas. Environmental toxicology, 28 (12), 689–699.
   PubMed Web of Science ®Google Scholar
• Gökalp Muranli, F.D., et al., 2015. Genotoxic effects of diazinon on human peripheral blood lymphocytes. Arhiv Za Higijenu Rada i Toksikologiju, 66, 153–158.
   PubMed Web of Science ®Google Scholar
• Grandi, C., et al., 2006. Impiego del comet test in medicina del lavoro e tossicologia industriale: considerazioni e prospettive. Giornale Italiano Di Medicina Del Lavoro Ed Ergonomia, 28 (1), 5–13.
   PubMedGoogle Scholar
• Gunasekarana, V., et al., 2015. A comprehensive review on clinical applications of comet assay. Journal of clinical and diagnostic research, 9 (3), GE01–GE05.
   PubMed Web of Science ®Google Scholar
• Hartmann, A., et al., 2003a. Recommendations for conducting the in vivo alkaline comet assay. Mutagenesis, 18 (1), 45–51.
   PubMed Web of Science ®Google Scholar
• Hartmann, A., et al., 2003b. Comparative study with the alkaline comet assay and the chromosome aberration test. Mutation research, 536, 27–38.
   PubMed Web of Science ®Google Scholar
• Heuser, V., et al., 2008. Influence of age and sex on the spontaneous DNA damage detected by micronucleus test and comet assay in mice peripheral blood cells. Cell biology international, 32 (10), 1223–1229.
   PubMed Web of Science ®Google Scholar
• How, V., et al., 2015. Characterization of risk factors for DNA damage among paddy farm worker exposed to mixtures of organophosphates. Archives environmental & occupational health, 70 (2), 102–190.
   PubMed Web of Science ®Google Scholar
• Jackson, S.P., 2002. Sensing and repairing DNA double-strand breaks. Carcinogenesis, 23, 687–696.
   PubMed Web of Science ®Google Scholar
• Jasso-Pineda, Y., et al., 2015. DNA damage in Mexican children living in high-risk contaminated scenarios. Science of the total environment, 518–519, 38–48.
   PubMed Web of Science ®Google Scholar
• Kasiotis, K.M., et al., 2012. Monitoring of systemic exposure to plant protection products and DNA damage in orchard workers. Toxicology letters, 210 (2), 182–188.
   PubMed Web of Science ®Google Scholar
• Kaur, R., et al., 2011. Evaluation of DNA damage in agricultural workers exposed to pesticides using single cell gel electrophoresis (comet) assay. Indian journal of human genetics, 17 (3), 179–187.
   PubMedGoogle Scholar
• Khayat, C.B., et al., 2013. Assessment of DNA damage in Brazilian workers occupationally exposed to pesticides: a study from Central Brazil. Environmental science and pollution research, 20 (10), 7334–7340.
   PubMed Web of Science ®Google Scholar
• Knudsen, L.E., et al., 1992. Induction of DNA repair synthesis in human monocytes/B-lymphocytes compared with T-lymphocytes after exposure to N93 acetoxy-N-acetylaminofluorene and dimethylsulfate in vitro. Carcinogenesis, 13, 1285–1287.
   PubMed Web of Science ®Google Scholar
• Krejci, L., et al., 2003. Mending the break: two DNA double-strand break repair machines in eukaryotes. Progress in nucleic acid research and molecular biology, 74, 159–201.
   PubMedGoogle Scholar
• Kvitko, K., et al., 2012. Susceptibility to DNA damage in workers occupationally exposed to pesticides, to tannery chemicals and to coal dust during mining. Genetics and molecular biology, 35 (4 suppl), 1060–1068.
   PubMed Web of Science ®Google Scholar
• Kwiatkowska, M., et al., 2017. DNA damage and methylation induced by glyphosate in human peripheral blood mononuclear cells (in vitro study)). Food and chemical toxicology: an international journal published for the British industrial biological research association, 105, 93–98.
   PubMed Web of Science ®Google Scholar
• Lebailly, P., et al., 2015. DNA damage in B and T lymphocytes of farmers during one pesticide spraying season. International archives of occupational and environmental health, 88, 963–972.
   PubMed Web of Science ®Google Scholar
• Lindahl, T., and Andersson, A., 1972. Rate of chain breakage at apurinic sites in double-stranded deoxyribonucleic acid. Biochemistry, 11, 3618–3623.
   PubMed Web of Science ®Google Scholar
• Møller, P., et al., 1996. Oxidative stress associated with exercise, psychological stress and life-style factors. Chemico-biological interactions, 102, 17–36.
   PubMed Web of Science ®Google Scholar
• Møller, P., and Wallin, H., 1998. Adduct formation, mutagenesis and nucleotide excision repair of DNA damage produced by reactive oxygen species and lipid peroxidation products. Mutation research, 410, 271–290.
   PubMed Web of Science ®Google Scholar
• Møller, P., et al., 2002. Sunlight-induced DNA damage in human mononuclear cells. Faseb journal, 16 (1), 45–53.
   PubMed Web of Science ®Google Scholar
• Møller, P., and Loft, S., 2010. Oxidative damage to DNA and lipids as biomarkers of exposure to air pollution. Environmental health perspectives, 118 (8), 1126–1136.
   PubMed Web of Science ®Google Scholar
• Muhammad, A., et al., 2016. Biomonitoring of toxic effects of pesticides in occupationally exposed individuals. Safety and health at work, 7, 156–160.
   PubMed Web of Science ®Google Scholar
• Neri, M., et al., 2015. Worldwide interest in the comet assay: a bibliometric study. Mutagenesis, 30 (1), 155–163.
   PubMed Web of Science ®Google Scholar
• Nowsheen, S., et al., 2012. Assaying DNA damage in hippocampal neurons using the comet assay. Journal of visualized experiments, 70, e50049.
   Google Scholar
• OECD. 2014. Test No. 489: In vivo mammalian alkaline comet assay. http://dx.doi.org/10.1787/9789264224179-en.
   Google Scholar
• Rajmohan, M., et al., 2015. In vivo autofluorescence spectroscopic study and evaluation of DNA damage by comet assay in smokers. Journal of clinical and diagnostic research, 9 (5), ZC16–ZC19.
   PubMed Web of Science ®Google Scholar
• Rojas, E., et al., 1999. Single cell gel electrophoresis assay: methodology and applications. Journal of chromatography: B, biomedical sciences and applications, 722, 225–254.
   PubMed Web of Science ®Google Scholar
• Saad-Hussein, A., et al., 2017. GSTP1 and XRCC1 polymorphisms and DNA damage in agricultural workers exposed to pesticides. Mutation research, 819, 20–25.
   PubMed Web of Science ®Google Scholar
• Santoro, R., et al., 2016. Comet assay in cancer chemoprevention. Methods in molecular biology, 1379, 99–105.
   PubMedGoogle Scholar
• Sasaky, Y.F., et al., 2000. The comet assay with multiple mouse organs: comparison of comet assay results and carcinogenicity with 208 chemicals selected from the IARC monographs and US NTP Carcinogenicity database. Critical reviews in toxicology, 30, 629–799.
   PubMed Web of Science ®Google Scholar
• Sies, H., 1985. Oxidative stress: introductory remarks. London: Academic Press; 1, 8.
   Google Scholar
• Simoniello, M.F., et al., 2010. Biomarkers of cellular reaction to pesticide exposure in a rural population. Biomarkers, 15 (1), 52–60.
   PubMed Web of Science ®Google Scholar
• Singh, N.P., et al., 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental cell research, 175 (1), 184–191.
   PubMed Web of Science ®Google Scholar
• Singh, N.P., 2016. The comet assay: reflections on its development, evolution and applications. Mutation research - reviews in mutation research, 767, 23–30.
   PubMed Web of Science ®Google Scholar
• Sultana Shaik, A., et al., 2016. Evaluation of cytotoxicity and genotoxicity of pesticide mixtures on lymphocytes. Toxicology mechanisms and methods, 26 (8), 588–594.
   PubMed Web of Science ®Google Scholar
• Thakur, S., et al., 2017. APE1 modulates cellular responses to organophosphate pesticide-induced oxidative damage in non-small cell lung carcinoma A549 cells. Molecular and cellular biochemistry, [Epub ahead of print]. doi: 10.1080/19338244.2014.910489.
   Web of Science ®Google Scholar
• Tice, R.R., et al., 1990. The single cell gel assay: a sensitive technique for evaluating intercellular differences in DNA damage and repair. Basic life science, 53, 291–301.
   PubMedGoogle Scholar
• Tice, R.R., et al., 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environmental and molecular mutagenesis, 35, 206–221.
   PubMed Web of Science ®Google Scholar
• Valverde, M., and Rojas, E., 2009. Environmental and occupational biomonitoring using the comet assay. Mutation research, 681 (1), 93–109.
   PubMed Web of Science ®Google Scholar
• Van Gent, D.C., et al., 2001. Chromosomal stability and the DNA double-stranded break connection. Nature reviews genetics, 2, 196–206.
   PubMed Web of Science ®Google Scholar
• Varona-Uribe, M.E., et al., 2016. Exposure to pesticide mixtures and DNA damage among rice field workers. Archives of environmental & occupational health, 71 (1):3–9.
   PubMed Web of Science ®Google Scholar
• Vazquez Boucard, C., et al., 2017. A study of DNA damage in buccal cells of consumers of well- and/or tap-water using the comet assay: assessment of occupational exposure to genotoxicants. Environmental and Molecular Mutagenesis, 58 (8), 619–627.
   PubMed Web of Science ®Google Scholar
• Villarini, M., et al., 2015. Primary DNA damage in welders occupationally exposed to extremely-low-frequency magnetic fields (ELF-MF). Ann ig, 27 (3), 511–519.
   PubMed Web of Science ®Google Scholar
• Wang, Q.L., et al., 2013. Risk assessment of mouse gastric tissue cancer induced by dichlorvos and dimethoate. Oncology letters, 5 (4), 1385–1389.
   PubMed Web of Science ®Google Scholar
• Waters, D.J., et al. 2007. Non-invasive prediction of prostatic DNA damage by oxidative stress challenge of peripheral blood lymphocytes. Cancer epidemiology biomarkers & prevention, 16(9), 1906–1910.
   PubMed Web of Science ®Google Scholar
• Wilhelm, C.M., et al., 2015. Assessment of DNA damage in floriculturists in southern Brazil. Environmental science and pollution research international, 22, 8182–8189.
   PubMed Web of Science ®Google Scholar
• Zeljezic, D., et al., 2017. The effect of insecticides chlorpyrifos, α-cypermethrin and imidacloprid on primary DNA damage, TP 53 and c-Myc structural integrity by comet-FISH assay. Chemosphere, 182, 332–338.
   PubMed Web of Science ®Google Scholar
• Zepeda-Arce, R., et al., 2017. Oxidative stress and genetic damage among workers exposed primarily to organophosphate and pyrethroid pesticides. Environmental toxicology, 32 (6), 1754–1764.
   PubMed Web of Science ®Google Scholar