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Research Article

Cytotoxic activity of standardized extracts, a fraction, and individual secondary metabolites from fenugreek seeds against SKOV-3, HeLa and MOLT-4 cell lines

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Pages 422-435 | Received 16 Nov 2020, Accepted 09 Mar 2021, Published online: 13 Apr 2021

References

  • Aktas HG, Altun S. 2015. In vitro antitumor effects of a new cultivar (Gürarslan) of Trigonella foenum graecum L. Afr J Trad Compl Alt Med. 12(3):113–119.
  • Al-Daghri NM, Alokail MS, Alkharfy KM, Mohammed AK, Abd-Alrahman SH, Yakout SM, E Amer O, Krishnaswamy S. 2012. Fenugreek extract as an inducer of cellular death via autophagy in human T lymphoma Jurkat cells. BMC Complement Altern Med. 12:202–209.
  • Al-Oqail MM, Farshori NN, Al-Sheddi ES, Musarrat J, Al-Khedhairy AA, Siddiqui MA. 2013. In vitro cytotoxic activity of seed oil of fenugreek against various cancer cell lines. Asian Pac J Cancer Prev. 14(3):1829–1832.
  • Alsemari A, Alkhodairy F, Aldakan A, Al-Mohanna M, Bahoush E, Shinwari Z, Alaiya A. 2014. The selective cytotoxic anti-cancer properties and proteomic analysis of Trigonella foenum-graecum. BMC Complement Altern Med. 14:114–117.
  • Alshatwi AA, Shafi G, Hasan TN, Syed NA, Khoja KK. 2013. Fenugreek induced apoptosis in breast cancer MCF-7 cells mediated independently by fas receptor change. Asian Pac J Cancer Prev. 14(10):5783–5788.
  • Amin A, Alkaabi A, Al-Falasi S, Daoud SA. 2005. Chemopreventive activities of Trigonella foenum graecum (Fenugreek) against breast cancer. Cell Biol Int. 29(8):687–694.
  • Aumsuwan P, Khan SI, Khan IA, Ali Z, Avula B, Walker LA, Shariat-Madar Z, Helferich WG, Katzenellenbogen BS, Dasmahapatra AK. 2016. The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro. Arch Biochem Biophys. 591:98–110.
  • Babaei F, Moafizad A, Darvishvand Z, Mirzababaei M, Hosseinzadeh H, Nassiri-Asl M. 2020. Review of the effects of vitexin in oxidative stress-related diseases. Food Sci Nutr. 8(6):2569–2580.
  • Bahmani M, Shirzad H, Mirhosseini M, Mesripour A, Rafieian-Kopaei M. 2016. A review on ethnobotanical and therapeutic uses of fenugreek (Trigonella foenum-graceum L). J Evid Based Complementary Altern Med. 21(1):53–62.
  • Bai Y, Qu XY, Yin JQ, Wu L, Jiang H, Long HW, Jia Q. 2014. Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in A549 human lung cancer cells. Pharmacogn Mag. 10(39):318–324.
  • Bhardwaj M, Paul S, Jakhar R, Khan I, Kang JI, Kim HM, Yun JW, Lee S-J, Cho HJ, Lee HG, et al. 2017. Vitexin confers HSF-1 mediated autophagic cell death by activating JNK and ApoL1 in colorectal carcinoma cells. Oncotarget. 8(68):112426–112441.
  • Boik J. 2001. Natural Compounds in Cancer Therapy. Princeton (MN): Oregon Medical Press.
  • Chen J, Li HM, Zhang XN, Xiong CM, Ruan JL. 2014. Dioscin-induced apoptosis of human LNCaP prostate carcinoma cells through activation of caspase-3 and modulation of Bcl-2 protein family. J Huazhong Univ Sci Technolog Med Sci. 34(1):125–130.
  • Chen Y, Tang YM, Yu SL, Han UW, Kou JP, Liu BL, Yu BY. 2015. Advances in the pharmacological activities and mechanisms of diosgenin. Chin J Nat Med. 13(8):578–587.
  • Corbiere C, Liagre B, Bianchi A, Bordji K, Dauça M, Netter P, Beneytout JL. 2003. Different contribution of apoptosis to the antiproliferative effects of diosgenin and other plant steroids, hecogenin and tigogenin, on human 1547 osteosarcoma cells. Int J Oncol. 22(4):899–905.
  • Corbiere C, Liagre B, Terro F, Beneytout JL. 2004. Induction of antiproliferative effect by diosgenin through activation of p53, release of apoptosis-inducing factor (AIF) and modulation of caspase-3 activity in different human cancer cells. Cell Res. 14(3):188–196.
  • El Bairi K, Ouzir M, Agnieszka N, Khalki L. 2017. Anticancer potential of Trigonella foenum graecum: Cellular and molecular targets. Biomed Pharmacother. 90:479–491.
  • Grądzka I. 2006. Mechanizmy i regulacja programowanej śmierci komórek [Mechanisms and regulation of the programmed cell death]. Postepy Biochem. 52:157–165.
  • Guo Q, Tian X, Yang A, Zhou Y, Wu D, Wang Z. 2014. Orientin in Trollius chinensis Bunge inhibits proliferation of HeLa human cervical carcinoma cells by induction of apoptosis. Monatsh Chem. 145(1):229–233.
  • Hibasami H, Moteki H, Ishikawa K, Katsuzaki H, Imai K, Yoshioka K, Ishii Y, Komiya T. 2003. Protodioscin isolated from fenugreek (Trigonella foenum-graecum L.) induces cell death and morphological change indicative of apoptosis in leukemic cell line H-60, but not in gastric cancer cell line KATO III. Int J Mol Med. 1:23–26.
  • Hordyjewska A, Pasternak K. 2005. Apoptotic death of the cell. Adv Clin Exp Med. 14:545–554.
  • Hsieh MJ, Tsai TL, Hsieh YS, Wang CJ, Chiou HL. 2013. Dioscin-induced autophagy mitigates cell apoptosis through modulation of PI3K/Akt and ERK and JNK signaling pathways in human lung cancer cell lines. Arch Toxicol. 87(11):1927–1937.
  • Hu M, Xu L, Yin L, Qi Y, Li H, Xu Y, Han X, Peng J, Wan X. 2013. Cytotoxicity of dioscin in human gastric carcinoma cells through death receptor and mitochondrial pathways. J Appl Toxicol. 33(8):712–722.
  • Huo R, Zhou QL, Wang BX, Tashiro SI, Onodera S, Ikejima T. 2004. Diosgenin induces apoptosis in HeLa cells via activation of caspase pathway. Acta Pharmacol Sin. 25:1077–1082.
  • Jabeen A, Rani S, Ibrahim M. 2018. Pharmacognostic and therapeutic importance of fenugreek (Trigonella foenum-graecum L.). Indo. Am J Pharm Sci. 05:5253–5262.
  • Khalil MIM, Ibrahim MM, El-Gaaly GA, Sultan AS. 2015. Trigonella foenum (Fenugreek) induced apoptosis in hepatocellular carcinoma cell line, HepG2, mediated by upregulation of p53 and proliferating cell nuclear antigen. Biomed Res Int. 2015:914645–914611.
  • Khoja KK, Shaf G, Hasan TN, Syed NA, Al-Khalifa AS, Al-Assaf AH, Alshatwi AA. 2011. Fenugreek, a naturally occurring edible spice, kills MCF-7 human breast cancer cells via an apoptotic pathway. Asian Pac J Cancer Prev. 12(12):3299–3304.
  • Kim EA, Jang JH, Lee YH, Sung EG, Song IH, Kim JY, Kim S, Sohn HY, Lee TJ. 2014. Dioscin induces caspase-independent apoptosis through activation of apoptosis-inducing factor in breast cancer cells. Apoptosis. 19(7):1165–1175.
  • Korzeniewska-Dyl I. 2007. Caspases – structure and function. Pol Merkur Lekarski. 23(138):403–407.
  • Korzeniewska-Dyl I. 2008. Caspases – a new target in inflammation and cancer therapy? Pol Merkur Lekarski. 24(139):5–7.
  • Król-Kogus B, Głód D, Krauze-Baranowska M. 2020. Qualitative and quantitative HPLC-ELSD-ESI-MS analysis of steroidal saponins in fenugreek seed. Acta Pharm. 70(1):89–99.
  • Król-Kogus B, Hałasa R, Głód D, Krauze-Baranowska M, Pobłocka-Olech L. 2021. 2D LC as a tool for standardization of Foenugraeci semen extracts containing compounds with anti-Helicobacter pylori activity. Food Funct. DOI:10.1039/D1FO00226K.
  • Kustermann S, Boess F, Buness A, Schmitz M, Watzele M, Weiser T, Singer T, Suter L, Roth A. 2013. A label-free, impedance-based real time assay to identify drug-induced toxicities and differentiate cytostatic from cytotoxic effects. Toxicol in Vitro. 27(5):1589–1595.
  • Łabe¸dzka K, Grzanka A, Izdebska M. 2006. Mitochondria and cell death. Postepy Hig Med Dosw. 60:439–446.
  • Lee CY, Chien YS, Chiu TH, Huang WW, Lu CC, Chiang JH, Yang JS. 2012. Apoptosis triggered by vitexin in U937 human leukemia cells via a mitochondrial signaling signaling pathway. Oncol Rep. 28(5):1883–1888.
  • Lepage C, Léger DY, Bertrand J, Martin F, Beneytout JL, Liagre B. 2011. Diosgenin induces death receptor-5 through activation of p38 pathway and promotes TRAIL-induced apoptosis in colon cancer cells. Cancer Lett. 301(2):193–202.
  • Li F, Fernandez PP, Rajendran P, Hui KM, Sethi G. 2010. Diosgenin, a steroidal saponin, inhibits STAT3 signaling pathway leading to suppression of proliferation and chemosensitization of human hepatocellular carcinoma cells. Cancer Lett. 292(2):197–207.
  • Li Y, Wang X, Cheng S, Du J, Deng Z, Zhang Y, Liu Q, Gao J, Cheng B, Ling C. 2015. Diosgenin induces G2/M cell cycle arrest and apoptosis in human hepatocellular carcinoma cells. Oncol Rep. 33(2):693–698.
  • Lin J, Tan YF, Ma TL, Ge ZJ, Wu YY, Ding WL, Feng JK, Jiang GJ, Shi GZ, Tang ZA. 2013. Diosgenin regulates proliferation, apoptosis, migration and invasion of human esophageal cancer eca109 cells via the MAPK signaling pathway. WCJD. 21(35):3977–3982.
  • Liu MJ, Wang Z, Ju Y, Wong RNS, Wu QY. 2005. Diosgenin induces cell cycle arrest and apoptosis in human leukemia K562 cells with the disruption of Ca2+ homeostasis. Cancer Chemother Pharmacol. 55(1):79–90.
  • Liu MJ, Yue PYK, Wang Z, Wong RNS. 2005. Methyl protodioscin induces G2/M arrest and apoptosis in K562 cells with the hyperpolarization of mitochondria. Cancer Lett. 224(2):229–241.
  • Lu Y, Luo J, Huang X, Kong L. 2009. Four new steroidal glycosides from Solanum torvum and their cytotoxic activities. Steroids. 74(1):95–101.
  • Lv L, Zheng L, Dong D, Xu L, Yin L, Xu Y, Qi Y, Han X, Peng J. 2013. Dioscin, a natural steroid saponin, induces apoptosis and DNA damage through reactive oxygen species: a potential new drug for treatment of glioblastoma multiforme. Food Chem Toxicol. 59:657–669.
  • Ly JD, Grubb DR, Lawen A. 2003. The mitochondrial membrane potential (Δψ m) in apoptosis; an update. Apoptosis. 8(2):115–128.
  • Melo PS, De Azevedo MBM, Zullo MAT, Fabrin-Neto JB, Haun M. 2004. Cytotoxicity of the phytosterol diosgenin and its derivatives in rat cultured hepatocytes and V79 fibroblasts. Hum Exp Toxicol. 23(10):487–493.
  • Moalic S, Liagre B, Corbière C, Bianchi A, Dauça M, Bordji K, Beneytout JL. 2001. A plant steroid, diosgenin, induces apoptosis, cell cycle arrest and COX activity in osteosarcoma cells. FEBS Lett. 506(3):225–230
  • Nagaprashantha LD, Vatsyayan R, Singhal J, Fast S, Roby R, Awasthi S, Singhal SS. 2011. Anti-cancer effects of novel flavonoid vicenin-2 as a single agent and in synergistic combination with docetaxel in prostate cancer. Biochem Pharmacol. 82(9):1100–1109.
  • Nagulapalli Venkata KC, Swaroop A, Bagchi D, Bishayee A. 2017. A small plant with big benefits: Fenugreek (Trigonella foenum-graecum Linn.) for disease prevention and health promotion. Mol Nutr Food Res. 61(6):1600950.
  • Ohtsuki T, Koyano T, Kowithayakorn T, Sakai S, Kawahara N, Goda Y, Yamaguchi N, Ishibashi M. 2004. New chlorogenin hexasaccharide isolated from Agave fourcroydes with cytotoxic and cell cycle inhibitory activities. Bioorg Med Chem. 12(14):3841–3845.
  • Pena-Moran OA, Villarreal ML, Alvarez-Berber L, Meneses-Acosta A, Rodriguez-Lopez V. 2016. Cytotoxicity, post-treatment recovery and selectivity analysis of naturally occurring podophyllotoxins from Bursera fagaroides var. fagaroides on breast cancer cell lines. Molecules. 21(8):1013–1027.
  • Pettit GR, Doubek DL, Herald DL, Numata A, Takahasi C, Fujiki R, Miyamoto T. 1991. Isolation and structure of cytostatic steroidal saponins from the African medicinal plant Balanites aegyptica. J Nat Prod. 54(6):1491–1502.
  • Podolak I, Galanty A, Sobolewska D. 2010. Saponins as cytotoxic agents: a review. Phytochem Rev. 9(3):425–474.
  • Procházkova D, Boušová I, Wilhelmová N. 2011. Antioxidant and prooxidant properties of flavonoids. Fitoterapia. 82:513–523.
  • Quispe A, Zavala C, Rojas J, Posso M, Vaisberg A. 2006. Efecto citotoxico selectivo in vitro de murcin H (acetogenina de Annona muricata) en cultivos celulares de cancer de pulmon [Selective in vitro cytotoxic effect of muricin H (Annona muricataacetogenenin) in lung cancer cell cultures]. Rev Peru Med Exp. 23:265–269.
  • Raju J, Bird RP. 2007. Diosgenin, a naturally occurring steroid [corrected] saponin suppresses 3-hydroxy-3-methylglutaryl CoA reductase expression and induces apoptosis in HCT-116 human colon carcinoma cells. Cancer Lett. 255(2):194–204.
  • Raju J, Mehta R. 2009. Cancer chemopreventive and therapeutic effects of diosgenin, a food saponin. Nutr Cancer. 61(1):27–35.
  • Raju J, Patlolla JMR, Swamy MV, Rao CV. 2004. Diosgenin, a steroid saponin of Trigonella foenum-graecum (fenugreek), inhibits azoxymethane-induced aberrant crypt foci formation in F344 rats and induces apoptosis in HT-29 human colon cancer cells. Cancer Epidemiol Biomarkers Prev. 13:1392–1398.
  • Sak K. 2014. Cytotoxicity of dietary flavonoids on different human cancer types. Pharmacogn Rev. 8(16):122–146.
  • Sarkar MK, Mahapatra SK, Vadivel V. 2020. Oxidative stress mediated cytotoxicity in leukemia cells induced by active phyto-constituents isolated from traditional herbal drugs of West Bengal. J Ethnopharmacol. 251:112527.
  • Selim S, Al Jaouni S. 2015. Anticancer and apoptotic effects on cell proliferation of diosgenin isolated from Costus speciosus (Koen). BMC Complement Altern Med. 15(1):301–307.
  • Sethi G, Shanmugam MK, Warrier S, Merarchi M, Arfuso F, Kumar AP, Bishayee A. 2018. Pro-apoptotic and anti-cancer properties of diosgenin: a comprehensive and critical review. Nutrients. 10(5):645–656.
  • Shabbeer S, Sobolewski M, Anchoori RK, Kachhap S, Davidson N, Carducci MA, Khan SR. 2009. Fenugreek a naturally occurring edible spice as an anticancer agent. Canc Biol Ther. 8:268–274.
  • Si L, Zheng L, Xu L, Yin L, Han X, Qi Y, Xu Y, Wang C, Peng J. 2016. Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion. Eur J Pharmacol. 774:105–117.
  • Stefanowicz-Hajduk J, Bartoszewski R, Bartoszewska S, Kochan K, Adamska A, Kosiński I, Ochocka JR. 2015. Pennogenyl saponins from Paris quadrifolia L. induce extrinsic and intrinsic pathway of apoptosis in human cervical cancer HeLa cells. PLOS One. 10(8):e0135993–16.
  • Stefanowicz-Hajduk J, Sparzak-Stefanowska B, Krauze-Baranowska M, Ochocka JR. 2016. Securinine from Phyllanthus glaucus induces cell cycle arrest and apoptosis in human cervical cancer HeLa cells. PLOS One. 11(10):e0165372.
  • Stepień A, Izdebska M, Grzanka A. 2007. The types of cell death. Postepy Hig Med Dosw. 61:420–428.
  • Tong QY, He Y, Zhao QB, Qing Y, Huang W, Wu XH. 2012. Cytotoxicity and apoptosis-inducing effect of steroidal saponins from Dioscorea zingiberensis Wright against cancer cells. Steroids. 77(12):1219–1227.
  • Trouillas P, Corbière C, Liagre B, Duroux JL, Beneytout JL. 2005. Structure-function relationship for saponin effects on cell cycle arrest and apoptosis in the human 1547 osteosarcoma cells: a molecular modelling approach of natural molecules structurally close to diosgenin. Bioorg Med Chem. 13(4):1141–1149.
  • Waheed A, Barker J, Barton SJ, Owen CP, Ahmed S, Carew MA. 2012. A novel steroidal saponin glycoside from Fagonia indica induces cell-selective apoptosis or necrosis in cancer cells. Eur J Pharm Sci. 47(2):464–473.
  • Wang SL, Cai B, Cui CB, Liu HW, Wu CF, Yao XS. 2004. Diosgenin-3-O-alpha-L-rhamnopyranosyl-(1 -> 4)-beta-D-glucopyranoside obtained as a new anticancer agent from Dioscorea futschauensis induces apoptosis on human colon carcinoma HCT-15 cells via mitochondria-controlled apoptotic pathway. J Asian Nat Prod Res. 6(2):115–125.
  • Wang G, Chen H, Huang M, Wang N, Zhang J, Zhang Y, Bai G, Fong WF, Yang M, Yao X. 2006. Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in HepG2 liver cancer cells. Cancer Lett. 241(1):102–109.
  • Wang LJ, Wang Y, Chen SW, Ma JS, Fu Q, Wang BX. 2002. The antitumor activity of diosgenin in vivo and in vitro. Zhongguo Zhong Yao Za Zhi. 27(10):777–779.
  • Xiao J, Capanoglu E, Jassbi AR, Miron A. 2016. Advance on the flavonoid C-glycosides and health benefits. Crit Rev Food Sci Nutr. 56:S29–S45.
  • Yadav UCS, Baquer NZ. 2014. Pharmacological effects of Trigonella foenum-graecum L. in health and disease. Pharm Biol. 52(2):243–254.
  • Yao D, Zhang B, Zhu J, Zhang Q, Hu Y, Wang S, Wang Y, Cao H, Xiao J. 2020. Advances on application of fenugreek seeds as functional foods: pharmacology, clinical application, products, patents and market. Crit Rev Food Sci Nutr. 60(14):2342–2352.
  • Yen CT, Lee CL, Chang FR, Hwang TL, Yen HF, Chen CJ, Chen SL, Wu YC. 2012. Indiosides G-K: steroidal glycosides with cytotoxic and anti-inflammatory activities from Solanum violaceum. J Nat Prod. 75(4):636–643.
  • Yuan L, Wang J, Xiao H, Wu W, Wang Y, Liu X. 2013. MAPK signaling pathways regulate mitochondrial-mediated apoptosis induced by isoorientin in human hepatoblastoma cancer cells. Food Chem Toxicol. 53:62–68.
  • Yuan L, Wei S, Wang J, Liu X. 2014. Isoorientin induces apoptosis and autophagy simultaneously by reactive oxygen species (ROS)-Related p53, PI3K/Akt, JNK, and p38 signaling pathways in HepG2 cancer cells. J Agric Food Chem. 62(23):5390–5400.
  • Zhao X, Ren H, Gao S, Hao J. 2014. Effects of dioscin on apoptosis in pancreatic cancer MiaPaCa-2 cells and its mechanism. Zhonghua Zhong Liu Za Zhi. 36(1):5–10.
  • Zhu DX, An F, Wang SH. 2012. Effects of vitexin on cell growth and apoptosis of human esophageal cancer EC-109 cells. Chin Tradit Herbal Drugs. 43:1781–1784.