Simplified culture techniques for growth and differentiation of murine and human pre-adipocytes for translational applications

References

• Fraser JK, Wulur I, Alfonso Z, et al. Fat tissue: an underappreciated source of stem cells for biotechnology. Trends Biotechnol 2006; 24: 150–4
   PubMed Web of Science ®Google Scholar
• Gimble JM, Katz AJ, Bunnell BA. Adipose-derived stem cells for regenerative medicine. Circ Res 2007; 100: 1249–60
   PubMed Web of Science ®Google Scholar
• Helder MN, Knippenberg M, Klein-Nulend J, et al. Stem cells from adipose tissue allow challenging new concepts for regenerative medicine. Tissue Eng 2007; 13: 1799–808
   PubMedGoogle Scholar
• Goh BC, Thirumala S, Kilroy G, et al. Cryopreservation characteristics of adipose-derived stem cells: maintenance of differentiation potential and viability. J Tissue Eng Regen Med 2007; 1: 322–4
   PubMed Web of Science ®Google Scholar
• Parker AM, Shang H, Khurgel M, et al. Low serum and serum-free culture of multipotential human adipose stem cells. Cytotherapy 2007; 9: 637–46
   PubMed Web of Science ®Google Scholar
• Suga H, Shigeura T, Matsumoto D, et al. Rapid expansion of human adipose-derived stromal cells preserving multipotency. Cytotherapy 2007; 9: 738–45
   PubMed Web of Science ®Google Scholar
• Barngrover D, Thomas J, Thilly WG. High density mammalian cell growth in Leibovitz bicarbonate-free medium: effects of fructose and galactose on culture biochemistry. J Cell Sci 1985; 78: 173–89
   PubMedGoogle Scholar
• Mackenzie CG, Mackenzie JB, Beck P. The effect of pH on growth, proteins synthesis and lipid rich particles of cultured mammalian cells. J Biophys Biochem Cytol 1961; 9: 141–56
   PubMedGoogle Scholar
• Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hygiene 1963; 78: 173–80
   PubMed Web of Science ®Google Scholar
• Yang Y, Chen X, Ding F, et al. Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and cells in vitro. Biomaterials 2007; 28: 1643–52
   PubMed Web of Science ®Google Scholar
• Wen CM, Cheng YH, Huang YF, et al. Isolation and characterization of a neural progenitor cell line from tilapia brain. Comp Biochem Physiol A Mol Integr Physiol 2008; 49: 167–80
   Google Scholar
• Mitaka T, Norioka K, Mochizuki Y. Redifferentiation of proliferated rat hepatocytes cultured in L15 medium supplemented with EGF and DMSO. In Vitro Cell Dev Biol Anim 1993; 29A: 714–22
   PubMedGoogle Scholar
• Coundouris JA, Grant MH, Engeset J, et al. Cryopreservation of human adult hepatocytes for use in drug metabolism and toxicity studies. Xenobiotica 1993; 23: 1399–409
   PubMed Web of Science ®Google Scholar
• Fujii M, Yoshino I, Suzuki M, et al. Primary culture of chicken hepatocytes in serum-free medium (pH 7.8) secreted albumin and transferrin for a long period in free gas exchange with atmosphere. Int J Biochem Cell Biol 1996; 28: 1381–91
   PubMedGoogle Scholar
• Le Marrec-Croq F, Fritayre P, Chesné C, et al. Cryopreservation of Pecten maximus heart cells. Cryobiology 1998; 37: 200–6
   PubMedGoogle Scholar
• de Jesus Ribeiro C, Ohara MT, Gama P. Alternative model to human skin organ culture: a preliminary study with Leibovitz L15 medium. Microsc Res Tech 2005; 66: 139–44
   PubMedGoogle Scholar
• de Kreuk AM, Jonkhoff AR, Zevenbergen A, et al. Storage of unprocessed G-CSF-mobilized whole blood in a modified Leibovitz's L15 medium preserves clonogenic capacity for at least 7 days. Bone Marrow Transplant 2001; 28: 145–55
   PubMedGoogle Scholar
• Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 2001; 7: 211–26
   PubMedGoogle Scholar
• Ramirez-Zacarias JL, Castro-Muñozledo F, Kuri-Harcuch W. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipid with oil red O. Histochemistry 1992; 97: 493–7
   PubMedGoogle Scholar
• Salazar-Olivo LA, Castro-Muñozledo F, Kuri-Harcuch W. A preadipose 3T3 cell variant highly sensitive to adipogenic factors and to human growth hormone. J Cell Sci 1995; 108: 2101–7
   PubMedGoogle Scholar
• Morais C, Westhuyzen J, Pat B, et al. High ambient glucose is effect neutral on cell death and proliferation in human proximal tubular epithelial cells. Am J Physiol Renal Physiol 2005; 289: F401–9
   Web of Science ®Google Scholar
• Hems DA, Rath EA, Verrinder TR. Fatty acid synthesis in liver and adipose tissue of normal and genetically obese (ob/ob) mice during the 24-hour cycle. Biochem J 1975; 150: 167–73
   PubMedGoogle Scholar
• Aguiari P, Leo S, Zavan B, et al. High glucose induces adipogenic differentiation of muscle-derived stem cells. Proc Natl Acad Sci USA 2008; 105: 1226–31
   PubMed Web of Science ®Google Scholar
• Thompson KA, Kleinzeller A. Accumulation of some hexoses in adipocytes: properties of the system. Am J Physiol Cell Physiol 1989; 257: C214–22
   Google Scholar
• Green A, Rumberger JM, Stuart CA, et al. Stimulation of lipolysis by tumor necrosis factor-a in 3T3-L1 adipocytes is glucose dependent. Implications for long-term regulation of lipolysis. Diabetes 2004; 53: 74–81
   PubMed Web of Science ®Google Scholar
• Floyd ZE, Kilroy G, Wu X, et al. Effects of prolyl hydroxylase inhibitors on adipogenesis and hypoxia inducible factor 1 alpha levels under normoxic conditions. J Cell Biochem 2007; 101: 1545–57
   PubMed Web of Science ®Google Scholar
• Gentil C, Le Jan S, Philippe J, et al. Is oxygen a key factor in the lipodystrophy phenotype?. Lipids Health Dis 2006; 5: 27
   PubMedGoogle Scholar
• Lin Q, Lee Y-J, Yun Z. Differentiation arrest by hypoxia. J Biol Chem 2006; 281: 30678–83
   PubMed Web of Science ®Google Scholar