Quantitative detection of EP3-II, III and VI messenger RNA in gravid and non-gravid human myometrium using real-time RT-PCR

References

• Duchesne M J, Thaler-Dao H, de Paulet A C. Prostaglandin synthesis in human placenta and fetal membranes. Prostaglandins 1978; 15: 19–42
   PubMed Web of Science ®Google Scholar
• Olson D M, Smieja Z, Zakar T, MacLeod E A, Walton J, Milne K. Regulation of prostaglandin synthesis in the human amnion. Reprod Fertil Dev 1991; 3: 413–419
   PubMedGoogle Scholar
• Gibb W, Sun M. Localization of prostaglandin H synthase type 2 protein and mRNA in term human fetal membranes and decidua. J Endocrinol 1996; 150: 497–503
   PubMed Web of Science ®Google Scholar
• Coleman R A, Smith W L, Narumiya S. International union of pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes. Pharmacol Rev 1994; 46: 205–229
   PubMed Web of Science ®Google Scholar
• Funk C D, Furci L, FitzGerald G A, Grygorczyk R, Rochette C, Bayne M A, Abramovitz M, Adam M, Metters K M. Cloning and expression of a cDNA for the human prostaglandin E receptor EP1 subtype. J Biol Chem 1993; 268: 26767–26772
   PubMedGoogle Scholar
• Kotani M, Tanaka I, Ogawa Y, Usui T, Mori K, Ichikawa A, Narumiya S, Yoshimi T, Nakao K. Molecular cloning and expression of multiple isoforms of human prostaglandin E receptor EP3 subtype generated by alternative messenger RNA splicing: multiple second messenger systems and tissue-specific distributions. Mol Pharmacol 1995; 48: 869–879
   PubMed Web of Science ®Google Scholar
• Kotani M, Tanaka I, Ogawa Y, Suganami T, Matsumoto T, Muro S, Yamamoto Y, Sugawara A, Yoshimasa Y, Sagawa N, et al. Multiple signal transduction pathways through two prostaglandin E receptor EP3 subtype isoforms expressed in human uterus. J Clin Endocrinol Metab 2000; 85: 4315–4322
   PubMedGoogle Scholar
• Bastien L, Sawyer N, Grygorczyk R, Metters K M, Adam M. Cloning, functional expression, and characterization of the human prostaglandin E2 receptor EP2 subtype. J Biol Chem 1994; 269: 11873–11877
   PubMedGoogle Scholar
• Regan J W, Bailey T J, Pepperl D J, Pierce K L, Bogardus A M, Donello J E, Fairbairn C E, Kedzie K M, Woodward D F, Gill D W. Cloning of a novel human prostaglandin receptor with characteristics of the pharmacologically defined EP2 subtype. Mol Pharmacol 1994; 46: 213–220
   PubMed Web of Science ®Google Scholar
• Namba T, Sugimoto Y, Negishi M, Irie A, Ushikubi F, Kakizuka A, Ito S, Ichikawa A, Narumiya S. Alternative splicing of C-terminal tail of prostaglandin E receptor subtype EP3 determines G-protein specificity. Nature 1993; 365: 166–170
   PubMed Web of Science ®Google Scholar
• Irie A, Sugimoto Y, Namba T, Harazono A, Honda A, Watabe A, Negishi M, Narumiya S, Ichikawa A. Third isoform of the prostaglandin-E-receptor EP3 subtype with different C-terminal tail coupling to both stimulation and inhibition of adenylate cyclase. Eur J Biochem 1993; 217: 313–318
   PubMedGoogle Scholar
• Irie A, Sugimoto Y, Namba T, Asano T, Ichikawa A, Negishi M. The C-terminus of the prostaglandin-E-receptor EP3 subtype is essential for activation of GTP-binding protein. Eur J Biochem 1994; 224: 161–166
   PubMedGoogle Scholar
• Schmid A, Thierauch K H, Schleuning W D, Dinter H. Splice variants of the human EP3 receptor for prostaglandin E2. Eur J Biochem 1995; 228: 23–30
   PubMedGoogle Scholar
• Kotani M, Tanaka I, Ogawa Y, Usui T, Tamura N, Mori K, Narumiya S, Yoshimi T, Nakao K. Structural organization of the human prostaglandin EP3 receptor subtype gene (PTGER3). Genomics 1997; 40: 425–434
   PubMed Web of Science ®Google Scholar
• Wing D A, Goharkhay N, Hanna M, Naidu Y M, Kovacs B W, Felix J C. EP3-2 receptor mRNA expression is reduced and EP3-6 receptor mRNA expression is increased in gravid human myometrium. J Soc Gynecol Investig 2003; 10: 124–129
   PubMedGoogle Scholar
• Astle S, Thornton S, Slater D M. Identification and localization of prostaglandin E2 receptors in upper and lower segment human myometrium during pregnancy. Mol Hum Reprod 2005; 11: 279–287
   PubMed Web of Science ®Google Scholar
• Vandenbroucke I I, Vandesompele J, Paepe A D, Messiaen L. Quantification of splice variants using real-time PCR. Nucleic Acids Res 2001; 29: E68
   PubMedGoogle Scholar
• Walton H S, Gebhardt F M, Innes D J, Dodd P R. Analysis of multiple exon-skipping mRNA splice variants using SYBR Green real-time RT-PCR. J Neurosci Methods 2007; 160: 294–301
   PubMedGoogle Scholar
• Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001; 25: 402–408
   PubMed Web of Science ®Google Scholar
• Smith G C, Wu W X, Nijland M J, Koenen S V, Nathanielsz P W. Effect of gestational age, corticosteroids, and birth on expression of prostanoid EP receptor genes in lamb and baboon ductus arteriosus. J Cardiovasc Pharmacol 2001; 37: 697–704
   PubMedGoogle Scholar
• Leonhardt A, Glaser A, Wegmann M, Hackenberg R, Nusing R M. Expression of prostanoid receptors in human lower segment pregnant myometrium. Prostaglandins Leukot Essent Fatty Acids 2003; 69: 307–313
   PubMedGoogle Scholar
• Grigsby P L, Sooranna S R, Adu-Amankwa B, Pitzer B, Brockman D E, Johnson M R, Myatt L. Regional expression of prostaglandin E2 and F2alpha receptors in human myometrium, amnion, and choriodecidua with advancing gestation and labor. Biol Reprod 2006; 75: 297–305
   PubMed Web of Science ®Google Scholar
• Lye S, Ou C, Teoh T, Erb G, Stevens Y, Casper R, Patel F, Challis J. The molecular basis of labour and tocolysis. Fet Mat Med Rev 1998; 10: 121–136
   Google Scholar