What we know and what we don’t know about perinatal Zika virus infection: a systematic review

References

• Oliveira Melo AS, Malinger G, Ximenes R, et al. Zika virus intrauterine infection causes fetal brain abnormality and microcephaly: tip of the iceberg? Ultrasound Obstet Gynecol. 2016 Jan;47(1):6–7.
   PubMed Web of Science ®Google Scholar
• Schuler-Faccini L, Ribeiro EM, Feitosa IM, et al. Possible association between Zika virus infection and microcephaly – Brazil, 2015. MMWR Morb Mortal Wkly Rep. 2016 Jan 29;65(3):59–62.
   PubMed Web of Science ®Google Scholar
• Mlakar J, Korva M, Tul N, et al. Zika virus associated with microcephaly. N Engl J Med. 2016;374(951–8):951–958.
   PubMedGoogle Scholar
• Microcephaly Epidemic Research Group. Microcephaly in infants, Pernambuco State, Brazil, 2015. Emerg Infect Dis. 2016;22(6): 1090–1093.
   PubMed Web of Science ®Google Scholar
• Baud D, Gubler DJ, Schaub B, et al. An update on Zika virus infection. Lancet. 2017 Nov 4;390(10107):2099–2109.
   PubMed Web of Science ®Google Scholar
• Dos Santos T, Rodriguez A, Almiron M, et al. Zika virus and the Guillain-Barré syndrome – case series from seven countries. N Engl J Med. 2016 Oct 20;375(16):1598–1601.
   PubMed Web of Science ®Google Scholar
• Parra B, Lizarazo J, Jiménez-Arango JA, et al. Guillain-Barré syndrome associated with Zika virus infection in Colombia. N Engl J Med. 2016 Oct 20;375(16):1513–1523.
   PubMed Web of Science ®Google Scholar
• Frontera JA, da Silva IR. Zika getting on your nerves? The association with the Guillain-Barré syndrome. N Engl J Med. 2016 Oct 20;375(16):1581–1582.
   PubMed Web of Science ®Google Scholar
• WHO statement on the first meeting of the International Health Regulations (2005). Emergency Committee on Zika virus and observed increase in neurological disorders and neonatal malformations, 1 February 2016. http://www.who.int/mediacentre/news/statements/2016/emergency-committee-zika-microcephaly/en/
   Google Scholar
• Schuler-Faccini L, Sanseverino M, Vianna F, et al. Zika virus: a new human teratogen? Implications for women of reproductive age. Clin Pharmacol Ther. 2016 Jul; 100(1):28–30. Epub 2016 May 13. PubMed PMID: 27090438: (http://apps.who.int/iris/bitstream/10665/254714/1/zikasitrep10Mar17-eng.pdf
   PubMed Web of Science ®Google Scholar
• Moher D, Liberati A, Tetzlaff J, Altman DG, The PRISMA Group (2009). Preferred Reporting Items for Systematic Reviews and Meta-Analyses: The PRISMA Statement. BMJ 2009;339:b2535, doi: 10.1136/bmj.b2535.
   Google Scholar
• Pan American Health Organization / World Health Organization. Zika Epidemiological Update, 25 August 2017. Washington, D.C.: PAHO/WHO; 2017.
   Google Scholar
• Haddow AD, Schuh AJ, Yasuda CY, et al. Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage. PLoS Negl Trop Dis. 2012;6(2):e1477.
   PubMed Web of Science ®Google Scholar
• Besnard M, Lastere S, Teissier A, et al. Evidence of perinatal transmission of Zika virus, French Polynesia, December 2013 and February 2014. Euro Surveill. 2014;19:20751.
   PubMedGoogle Scholar
• Cavalcanti MG, Cabral-Castro MJ, Gonçalves JLS, et al. Zika virus shedding in human milk during lactation. An unlikely source of infecton? Int J Infect Dis. 2017;57:70–72.
   PubMed Web of Science ®Google Scholar
• US Food and Drug Administration. Recommendations for donor screening, deferral, and product management to reduce the risk of transfusion transmission of Zika virus, recommendations for industry. 2016 Feb 16 [cited 2016 Feb 16]. http://www.fda.gov/downloads/BiologicsBloodVaccines/GuidanceComplianceRegulatoryInformation/Guidances/Blood/UCM486360.pdf
   Google Scholar
• Musso D, Nhan T, Robin E, et al. Potential for Zika virus transmission through blood transfusion demonstrated during an outbreak in French Polynesia, November 2013 to February 2014. Euro Surveill. 2014;19:20761.
   PubMedGoogle Scholar
• Brent C, Dunn A, Savage H, et al. Preliminary findings from an investigation of Zika virus infection in a patient with no known risk factors – Utah, 2016. MMWR. 2016 Sep 16;65:36.
   PubMed Web of Science ®Google Scholar
• Krow-Lucal ER, Novosad SA, Dunn AC, et al. Zika virus infection in patient with no known risk factors, Utah, USA, 2016. Emerg Infect Dis. 2017 Aug;23(8):1260–1267.
   PubMed Web of Science ®Google Scholar
• Swaminathan S, Schlaberg R, Lewis J, et al. Fatal Zika virus infection with secondary nonsexual transmission. N Engl J Med. 2016 Nov 10;375(19):1907–1909.
   PubMed Web of Science ®Google Scholar
• Newman CM, Dudley DM, Aliota MT, et al. Oropharyngeal mucosal transmission of Zika virus in rhesus macaques. Nat Commun. 2017 Aug 1;8(1):169.
   PubMed Web of Science ®Google Scholar
• Krow-Lucal ER, Biggerstaff BJ, Staples JE. Estimated incubation period for Zika virus disease. Emerg Infect Dis. 2017 May;23(5):841–844.
   PubMed Web of Science ®Google Scholar
• Aubry M, Teissier A, Huart M, et al. Zika Virus seroprevalence, French Polynesia, 2014–2015. Emerg Infect Dis. 2017 Apr;23(4):669–672.
   PubMed Web of Science ®Google Scholar
• Lustig Y, Mendelson E, Paran N, et al. Detection of Zika virus RNA in whole blood of imported Zika virus disease cases up to 2 months after symptom onset, Israel, December 2015 to April 2016. Euro Surveill. 2016;21:26.
   Google Scholar
• Gallian P, Cabié A, Richard P, et al. Zika virus in asymptomatic blood donors in Martinique. Blood. 2017 Jan 12;129(2):263–266.
   PubMed Web of Science ®Google Scholar
• Grubaugh ND, Ladner JT, Kraemer MUG, et al. Genomic epidemiology reveals multiple introductions of Zika virus into the United States. Nature. 2017 Jun 15;546(7658):401–405.
   PubMed Web of Science ®Google Scholar
• Faria NR, Quick J, Claro IM. Establishment and cryptic transmission of Zika virus in Brazil and the Americas. Nature. 2017 Jun 15;546(7658):406–410.
   PubMed Web of Science ®Google Scholar
• Metsky HC, Matranga CB, Wohl S, et al. Zika virus evolution and spread in the Americas. Nature. 2017 Jun 15;546(7658):411–415.
   PubMed Web of Science ®Google Scholar
• Meertens L, Labeau A, Dejamac O, et al. Axl mediates ZIKA virus entry in human glial cells and modulates innate immune responses. Cell Reports. 2016;18:1–10.
   Web of Science ®Google Scholar
• Fernandez-García MD, Mazzon M, Jacobs M, et al. Pathogenesis of flavivirus infections: using and abusing the host cell. Cell Host Microbe. 2009 Apr 23;5(4):318–328.
   PubMed Web of Science ®Google Scholar
• Hirsch AJ, Smith JL, Haese NN, et al. Zika virus infection of rhesus macaques leads to viral persistence in multiple tissues. PLoS Pathog. 2017;13(3):e1006219.
   PubMed Web of Science ®Google Scholar
• Dang J, Tiwari SK, Lichinchi G, et al. Zika virus depletes neural progenitors in human cerebral organoids through activation of the innate immune receptor TLR3. Cell Stem Cell. 2016;19:1–8.
   PubMed Web of Science ®Google Scholar
• Carroll T, Lo M, Lanteri M, et al. Zika virus preferentially replicates in the female reproductive tract after vaginal inoculation of rhesus macaques. PLOS Pathogens. 2017;13(7):e1006537.
   PubMed Web of Science ®Google Scholar
• Mysorekar IU, Diamond MS. Modeling Zika virus infection in pregnancy. N Engl J Med. 2016 Aug 4;375(5):481–484.
   PubMed Web of Science ®Google Scholar
• Adibi JJ, Marques ET Jr, Cartus A, et al. Teratogenic effects of the Zika virus and the role of the placenta. Lancet. 2016 Apr 9;387(10027):1587–1590.
   PubMed Web of Science ®Google Scholar
• Yockey LJ, Varela L, Rakib T, et al. Vaginal exposure to Zika virus during pregnancy leads to fetal brain infection. Cell. 2016;166:1247–1256.
   PubMed Web of Science ®Google Scholar
• Moreira J, Peixoto TM, Siqueira AM, et al. Sexually acquired Zika virus: a systematic review. Clin Microbiol Infect. 2017 May;23(5):296–305. Epub 2017 Jan 3. Review. PubMed PMID: 28062314.
   PubMed Web of Science ®Google Scholar
• Barzon L, Pacenti M, Franchin E, et al. Infection dynamics in a traveller with persistent shedding of Zika virus RNA in semen for six months after returning from Haiti to Italy, January 2016. Euro Surveill. 2016 Aug 11;21(32). DOI:10.2807/1560-7917.ES.2016.21.32.30316
   Google Scholar
• Tang H, Hammack C, Ogden SC, et al. Zika virus infects human cortical neural progenitors and attenuates their growth. Cell Stem Cell. 2016 May 5;18(5):587–590.
   PubMed Web of Science ®Google Scholar
• Nowakowski TJ, Pollen AA, Di Lullo E, et al. Kriegstein A2. Expression analysis highlights AXL as a candidate Zika virus entry receptor in neural stem cells. Cell Stem Cell. 2016 May 5;18(5):591–596.
   PubMed Web of Science ®Google Scholar
• Garcez PP, Loiola EC, Madeiro da Costa R, et al. Zika virus impairs growth in human neurospheres and brain organoids. Science. 2016 May 13;352(6287):816–818.
   PubMed Web of Science ®Google Scholar
• Li C, Xu D, Ye Q, et al. Zika virus disrupts neural progenitor development and leads to microcephaly in mice. Cell Stem Cell. 2016 Nov 3;19(5):672.
   PubMed Web of Science ®Google Scholar
• Oh Y, Zhang F, Wang Y, et al. Zika virus directly infects peripheral neurons and induces cell death. Nat Neurosci. 2017 Sep;20(9):1209–1212.
   PubMed Web of Science ®Google Scholar
• Dang J, Tiwari SK, Lichinchi G, et al. Zika virus depletes neural progenitors in human cerebral organoids through activation of the innate immune receptor TLR3. Cell Stem Cell. 2016 Aug 4;19(2):258–265.
   PubMed Web of Science ®Google Scholar
• Cugola FR, Fernandes IR, Russo FB, et al. The Brazilian Zika virus strain causes birth defects in experimental models. Nature. 2016 Jun 9;534(7606):267–271.
   PubMed Web of Science ®Google Scholar
• Bhatnagar J, Rabeneck DB, Martines RB, et al. Zika virus RNA replication and persistence in brain and placental tissue. Emerg Infect Dis. 2017 Mar;23(3):405–414.
   PubMed Web of Science ®Google Scholar
• Li H, Saucedo-Cuevas L, Regla-Nava JA, et al. Zika virus infects neural progenitors in the adult mouse brain and alters proliferation. Cell Stem Cell. 2016 Nov 3;19(5):593–598.
   PubMed Web of Science ®Google Scholar
• Murray KO, Gorchakov R, Carlson AR, et al. Prolonged detection of Zika virus in vaginal secretions and whole blood. Emerg Infect Dis. 2017 Jan;23(1):99–101.
   PubMed Web of Science ®Google Scholar
• Lanteri MC, Kleinman SH, Glynn SA, et al. Zika virus: a new threat to the safety of the blood supply with worldwide impact and implications. Transfusion. 2016 Jul;56(7):1907–1914.
   PubMed Web of Science ®Google Scholar
• Suy A, Sulleiro E, Rodó C, et al. Prolonged Zika virus viremia during pregnancy. N Engl J Med. 2016 Dec 29;375(26):2611–2613.
   PubMed Web of Science ®Google Scholar
• Rodó C, Suy A, Sulleiro E, et al. In utero negativization of Zika virus in a case with serious central nervous system abnormalities. Clin Microbiol Infect. 2017 Oct 10;pii: S1198-743X (17)30539-6. DOI:10.1016/j.cmi.2017.09.022.
   PubMedGoogle Scholar
• Schaub B, Vouga M, Najioullah F, et al. Analysis of blood from Zika virus-infected fetuses: a prospective case series. Lancet Infect Dis. 2017 May;17(5):520–527.
   PubMed Web of Science ®Google Scholar
• Oliveira DB, Almeida FJ, Durigon EL, et al. Prolonged shedding of Zika virus associated with congenital infection. N Engl J Med. 2016 Sep 22;375(12):1202–1204.
   PubMed Web of Science ®Google Scholar
• Gaskell KM, Houlihan C, Nastouli E, et al. Persistent Zika virus detection in semen in a traveler returning to the United Kingdom from Brazil, 2016. Emerg Infect Dis. 2017 Jan;23(1):137–139.
   PubMed Web of Science ®Google Scholar
• Venturi G, Zammarchi L, Fortuna C, et al. An autochthonous case of Zika due to possible sexual transmission, Florence, Italy, 2014. Euro Surveill. 2016;21:8.
   Google Scholar
• D’Ortenzio E, Matheron S, Yazdanpanah Y, et al. Evidence of sexual transmission of Zika virus. N Engl J Med. 2016 Jun 2;374(22):2195–2198.
   PubMed Web of Science ®Google Scholar
• Frank C, Cadar D, Schlaphof A, et al. Sexual transmission of Zika virus in Germany, April 2016. Euro Surveill. 2016;21:23.
   PubMedGoogle Scholar
• Govero J, Esakky P, Scheaffer SM, et al. Zika virus infection damages the testes in mice. Nature. 2016 Dec 15;540(7633):438–442.
   PubMed Web of Science ®Google Scholar
• Musso D, Richard V, Teissier A, et al. Detection of Zika virus RNA in semen of asymptomatic blood donors. Clin Microbiol Infect. 2017 Dec;23(12):1001.e1-1001.e3.
   PubMed Web of Science ®Google Scholar
• Cauchemez S, Besnard M, Bompard P, et al. Association between Zika virus and microcephaly in French Polynesia, 2013-15: a retrospective study. Lancet. 2016 May 21;387(10033):2125–2132.
   PubMed Web of Science ®Google Scholar
• Johansson MA, Mier-y-Teran-Romero L, Reefhuis J, et al. Zika and the risk of microcephaly. N Engl J Med. 2016 Jul 7;375(1):1–4.
   PubMed Web of Science ®Google Scholar
• Brasil P, Pereira JP Jr, Moreira ME, et al. Zika virus infection in pregnant women in Rio de Janeiro. N Engl J Med. 2016 Dec 15;375(24):2321–2334.
   PubMed Web of Science ®Google Scholar
• Shapiro-Mendoza CK, Rice ME, Galang RR, et al. Pregnancy outcomes after maternal Zika virus infection during pregnancy – U.S. Territories, January 1, 2016-April 25, 2017. MMWR Morb Mortal Wkly Rep. 2017 Jun 16;66(23):615–621.
   PubMed Web of Science ®Google Scholar
• Honein MA, Dawson AL, Petersen EE, et al. Birth defects among fetuses and infants of US women with evidence of possible Zika virus infection during pregnancy. JAMA. 2017 Jan 3;317(1):59–68.
   PubMed Web of Science ®Google Scholar
• Pacheco O, Beltrán M, Nelson CA, et al. Zika Virus disease in Colombia – preliminary report. N Engl J Med. 2016 Jun 15; 374.
   Google Scholar
• Halai UA, Nielsen-Saines K, Moreira ML, et al. Maternal Zika virus disease severity, virus load, prior dengue antibodies, and their relationship to birth outcomes. Clin Infect Dis. 2017 Sep 15;65(6):877–883.
   PubMed Web of Science ®Google Scholar
• van der Linden V, Pessoa A, Dobyns W, et al. Description of 13 infants born during October 2015-January 2016 with congenital Zika virus infection without microcephaly at Birth – Brazil. MMWR Morb Mortal Wkly Rep. 2016 Dec 2;65(47):1343–1348.
   PubMed Web of Science ®Google Scholar
• Villamil-Gómez WE, Rodríguez-Morales AJ, Uribe-García AM, et al. Zika, dengue, and chikungunya co-infection in a pregnant woman from Colombia. Int J Infect Dis. 2016Oct;51:135–138.
   PubMed Web of Science ®Google Scholar
• Moore CA, Staples JE, Dobyns WB, et al. Characterizing the pattern of anomalies in congenital Zika syndrome for pediatric clinicians. JAMA Pediatr. 2017 Mar 1;171(3):288–295.
   PubMed Web of Science ®Google Scholar
• Del Campo M, Feitosa IM, Ribeiro EM, et al. The phenotypic spectrum of congenital Zika syndrome. Am J Med Genet A. 2017 Apr;173(4):841–857.
   PubMed Web of Science ®Google Scholar
• Moura da Silva AA, Ganz JS, Sousa PD, et al. Early growth and neurologic outcomes of infants with probable congenital Zika virus syndrome. Emerg Infect Dis. 2016 Nov;22(11):1953–1956.
   PubMed Web of Science ®Google Scholar
• Leal MC, van der Linden V, Bezerra TP, et al. Characteristics of dysphagia in infants with microcephaly caused by congenital Zika virus infection, Brazil, 2015. Emerg Infect Dis. 2017 Aug;23(8):1253–1259.
   PubMed Web of Science ®Google Scholar
• França GV, Schuler-Faccini L, Oliveira WK, et al. Congenital Zika virus syndrome in Brazil: a case series of the first 1501 livebirths with complete investigation. Lancet. 2016 Aug 27;388(10047):891–897.
   PubMed Web of Science ®Google Scholar
• Leal MC, Muniz LF, Ferreira TS, et al. Hearing loss in infants with microcephaly and evidence of congenital Zika virus infection – Brazil, November 2015–May 2016. MMWR Morb Mortal Wkly Rep. 2016 Sep 2;65(34):917–919.
   PubMed Web of Science ®Google Scholar
• Cavalcanti DD, Alves LV, Furtado GJ, et al. Echocardiographic findings in infants with presumed congenital Zika syndrome: retrospective case series study. PLoS One. 2017 Apr 20;12(4):e0175065.
   PubMed Web of Science ®Google Scholar
• de Fatima Vasco Aragao M, van der Linden V, Brainer-Lima AM, et al. Clinical features and neuroimaging (CT and MRI) findings in presumed Zika virus related congenital infection and microcephaly: retrospective case series study. Bmj. 2016;353:i1901.
   PubMed Web of Science ®Google Scholar
• Soares de Oliveira-Szejnfeld P, Levine D, Melo AS, et al. Congenital brain abnormalities and Zika virus: what the radiologist can expect to see prenatally and postnatally. Radiology. 2016 Oct;281(1):203–218.
   PubMed Web of Science ®Google Scholar
• Vouga M, Baud D. Imaging of congenital Zika virus infection: the route to identification of prognostic factors. Prenat Diagn. 2016 Sep;36(9):799–811.
   PubMed Web of Science ®Google Scholar
• Mulkey SB, Vezina G, Bulas DI, et al. Neuroimaging findings in normocephalic newborns with intrauterine Zika virus exposure. Pediatr Neurol. 2018;78:75–78.
   PubMed Web of Science ®Google Scholar
• Chua A, Prat I, Nuebling CM, et al. Update on Zika diagnostic tests and WHO’s related activities. PLoS Negl Trop Dis. 2017 Feb 2;11(2):e0005269.
   PubMed Web of Science ®Google Scholar
• Stone M, Lanteri MC, Bakkour S, et al. Relative analytical sensitivity of donor nucleic acid amplification technology screening and diagnostic real-time polymerase chain reaction assays for detection of Zika virus RNA. Transfusion. 2017 Mar;57(3pt2):734–747.
   PubMedGoogle Scholar
• Ekins S, Liebler J, Neves BJ, et al. Illustrating and homology modeling the proteins of the Zika virus. F1000Research. 2016;5:275.
   PubMedGoogle Scholar
• Sirohi D, Chen Z, Sun L, et al. The 3.8 Å resolution cryo-EM structure of Zika virus. Science. 2016 Apr 22;352(6284):467–470.
   PubMed Web of Science ®Google Scholar
• Stettler K, Beltramello M, Espinosa DA, et al. Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection. Science. 2016 Aug 19;353(6301):823–826.
   PubMed Web of Science ®Google Scholar
• Balmaseda A, Stettler K, Medialdea-Carrera R, et al. Antibody-based assay discriminates Zika virus infection from other flaviviruses. Proc Natl Acad Sci USA. 2017 Aug 1;114(31):8384–8389.
   PubMed Web of Science ®Google Scholar
• Durbin AP. Dengue antibody and Zika: friend or foe? Trends Immunol. 2016;37(10):635–636.
   PubMed Web of Science ®Google Scholar
• Mor G, Aldo P, Alvero AB. The unique immunological and microbial aspects of pregnancy. Nat Rev Immunol. 2017 Aug;17(8):469–482.
   PubMed Web of Science ®Google Scholar
• Zhang B, et al. Diagnosis of Zika virus infection on a nanotechnology platform. Nat Med. 2017 May;23(5):548–550.
   PubMed Web of Science ®Google Scholar
• Pires de Mello CP, Tao X, Kim TH, et al. Zika virus (ZIKV) replication is substantially inhibited by novel favipiravir and interferon-alpha combination regimens. Antimicrob Agents Chemother. 2017;62:e01983–17, Epub ahead of print.
   PubMed Web of Science ®Google Scholar
• Snyder B, Goebel S, Koide F, et al. Synergistic antiviral activity of sofosbuvir and type -I interferons (α and β) against Zika virus. J Med Virol. 2018 Jan; 90(1):8-12.
   Google Scholar
• Ferreira AC, Zaverucha-do-Valle C, Reis PA, et al. Sofosbuvir protects Zika virus-infected mice from mortality, preventing short- and long-term sequelae. Sci Rep. 2017;7:9409.
   PubMed Web of Science ®Google Scholar
• Pitts JD, Li PC, de Wispelaere M, et al. Antiviral activity of N-(4-hydroxyphenyl) retinamide (4-HPR) against Zika virus. Antiviral Res. 2017;147: 124–130. Epub 2017 Oct 16.
   PubMed Web of Science ®Google Scholar
• Wang C, Yang SNY, Smith K, et al. Nuclear import inhibitor N-(4-hydroxyphenyl) retinamide targets Zika virus (ZIKV) nonstructural protein 5 to inhibit ZIKV infection. Biochem Biophys Res Commun. 2017;493: 1555–1559. Epub 2017 Oct 4.
   PubMed Web of Science ®Google Scholar
• Li C, Zhu X, Ji X, et al. Chloroquine, a FDA-approved drug, prevents Zika virus infection and its associated congenital microcephaly in Mice. EBioMedicine. 2017;24:189–194.
   PubMed Web of Science ®Google Scholar
• Xu M, Lee EM, Wen Z, et al. Identification of small molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen. Nat Med. 2016 Oct;22(10):1101–1107.
   PubMed Web of Science ®Google Scholar
• Julander JG, Siddhartan V, Evans J, et al. Efficacy of the broad-spectrum antiviral compound BCX4430 against Zika virus in cell culture and in a mouse model. Antiviral Res. 2017;137:14–22.
   PubMed Web of Science ®Google Scholar
• Yu Y, Deng YQ, Zou P, et al. A peptide-based viral inactivator inhibits Zika virus infection in pregnant mice and fetuses. Nat Commun. 2017;8:15672.
   PubMed Web of Science ®Google Scholar
• Wang J, Bardelli M, Espinosa DA, et al. A human bi-specific antibody against Zika virus with high therapeutic potential. Cell. 2017;171:229–241.
   PubMed Web of Science ®Google Scholar
• Cohen J. Infectious disease. The race for a Zika vaccine is on. Science. 2016;351:543–544.
   PubMed Web of Science ®Google Scholar
• Dowd KA, Ko SY, Morabito KM, et al. Rapid development of a DNA vaccine for Zika virus. Science. 2016;354:237–240.
   PubMed Web of Science ®Google Scholar
• Abbasi J. Zika vaccine enters clinical trials. JAMA. 2016;316:1249.
   Web of Science ®Google Scholar
• Abbink P, Larocca RA, De La Barrera RA, et al. Protective efficacy of multiple vaccine platforms against Zika virus challenge in rhesus monkeys. Science. 2016;353:1129–1132.
   PubMed Web of Science ®Google Scholar
• Larocca RA, Abbink P, Peron JP, et al. Vaccine protection against Zika virus from Brazil. Nature. 2016;536:474–478.
   PubMed Web of Science ®Google Scholar
• Barzon L, Palu G. Current views on Zika virus vaccine development. Expert Opin Biol Ther. 2017;17:1185–1192.
   PubMed Web of Science ®Google Scholar
• NIH. NIH begins testing investigational Zika vaccine in humans. NIH; 2016. Available from: https://www.nih.gov/news-events/news-releases/nih-beginstesting-investigational-zika-vaccine-humans
   Google Scholar
• NIH. Phase 2 Zika vaccine trials begins in US, Central and South America: study will evaluate NIH’s experimental DNA vaccine. Available from: https://www.nih.gov/news-events/news-releases/phase-2-zika-vaccine-trial-begins-us-central-south-america
   Google Scholar