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Pediatric Pathology & Laboratory Medicine Volume 16, 1996 - Issue 3
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Original Article

EWS/FLI-1 Fusion Transcript Detection and MIC2 Immunohistochemical Staining in the Diagnosis of Ewing's Sarcoma

C. S. Lee Department of Pathology, University of Melbourne, Parkville, Victoria, Australia
,
Melissa C. Southey Department of Anatomical Pathology, Royal Children's Hospital, Parkville, Victoria, Australia
,
Keith Waters Department of Oncology, Royal Children's Hospital, Parkville, Victoria, Australia
,
George Kannourakis LARCH Cancer Research Laboratory, Royal Children's Hospital, Parkville, Victoria, Australia
,
Toula Georgiou Department of Anatomical Pathology, St. Vincent's Hospital, Melbourne, Australia
,
Jane E. Armes Department of Anatomical Pathology, Austin Hospital, Heidelberg, Victoria, Australia
,
Chung Wo Chow Department of Anatomical Pathology, Royal Children's Hospital, Parkville, Victoria, Australia
&
Deon J. Venter Department of Anatomical Pathology, Royal Children's Hospital, Parkville, Victoria, Australia
 show all
Pages 379-392 | Received 15 Feb 1995, Accepted 07 Sep 1995, Published online: 16 Apr 2010

References

  • Wilkins R M, Pritchard D J, Burgert E O, Jr, Unni K K. Ewing's sarcoma of bone. Experience with 140 patients. Cancer 1986; 58: 2551–5
  • Dehner L P. Primitive neuroectodermal tumor and Ewing's sarcoma. Am J Surg Pathol 1993; 17: 1–13
  • Angervall L, Enzinger F M. Extraskeletal neoplasm resembling Ewing's sarcoma. Cancer 1975; 36: 240–51
  • Jaffe R, Santamaria M, Yunis E J, et al. The neuroectodermal tumor of bone. Am J Surg Pathol 1984; 8: 885–98
  • Turc-Carel C, Philip I, Berger M P, Philips T, Lenoir G. Chromosome study of Ewing's sarcoma (ES) cell lines. Consistency of a reciprocal translocation t(11;22)(q24;q12). Cancer Genet Cytogenet 1984; 12: 21–5
  • Turc-Carel C, Aurias A, Mugneret F, et al. Chromosomes in Ewing's sarcoma. I. An evaluation of 35 cases of remarkable consistency of t(11;22)(q24;q12). Cancer Genet Cytogenet 1988; 32: 229–38
  • Fellinger E J, Garin-Chesa P, Su S L, Deangelis P, Lane J M, Reitig W J. Biochemical and genetic characterization of the HBA71 Ewing's sarcoma cell surface antigen. Cancer Res 1991; 51: 336–40
  • Kovar H, Dworzak M, Strehl S, et al. Overexpression of the pseudoautosomal gene MIC2 in Ewing's sarcoma and peripheral primitive neuroectodermal tumor. Oncogene 1990; 5: 1067–70
  • Perlman E, Dickman P. Ewing's sarcoma—routine diagnostic utilization of MIC2 analysis. Hum Pathol 1994; 25: 304–7
  • Weidner N, Tjoe T. Immunohistochemical profile of monoclonal antibody O13. Am J Surg Pathol 1994; 18: 487–94
  • Ambros I M, Ambros P F, Strehl S, Kovar H, Gardner H, Salzer-Kuntschik M. MIC2 is a specific marker of Ewing's sarcoma and peripheral primitive neuroectodermal tumors. Evidence for a common histogenesis of Ewing's sarcoma and peripheral primitive neuroectodermal tumour from MIC2 expression and specific chromosome aberration. Cancer 1991; 67: 1886–93
  • Fellinger E J, Garin-Chesa P. Triche TJ, Huvos AG, Rettig WJ. Immunohistochemical analysis of Ewing's sarcoma cell surface antigen p30/32MIC2. Am J Pathol 1991; 139: 317–25
  • Biggs C, Crabbe J RD, Yavari F, Lou Y Q, Triche T J. MIC2 expression in small round cell tumours of childhood. Lab Invest 1993; 68: 126A
  • Riopel M A, Dickman P S, Link M, Perlman E J. MIC2 analysis in paediatric lymphomas and leukemias. Hum Pathol 1994; 25: 396–9
  • Ramani P, Rampling D, Link M. Immunocytochemical study of 12E7 in small round-cell tumours in childhood: An assessment of its sensitivity and specificity. Histopathology 1993; 23: 557–61
  • Delattre O, Zucman J, Plougastel B, et al. Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours. Nature 1992; 359: 162–5
  • Zucman J, Melot T, Desmaze C, et al. Combinatorial generation of variable fusion proteins in the Ewing family of tumors. EMBOJ 1993; 12: 4481–7
  • May W A, Gishizky M L, Lessnick S L, et al. Ewing sarcoma 11;22 translocation produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLU for transformation. Proc Natl Acad Sci USA 1993; 90: 5752–6
  • Downing J, Head D. Detection of the (11;22)(q24;q12) translocation of Ewing's sarcoma and peripheral neuroectodermal tumor by reverse transcriptase PCR. Am J Pathol 1993; 143: 1294–1300
  • Chomczynski P. Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162: 156–9
  • Brenner C A, Tarn A W, Nelson P A, et al. Message amplification phenotyping (MAPPing): A technique to simultaneously measure multiple mRNAs from small numbers of cells. Biotechniques 1989; 7: 1096–103
  • Zucman J, Delattre O, Desmaze C, et al. Cloning and characterization of the Ewing's sarcoma and peripheral neuroepithelioma t(11;22) translocation breakpoints. Genes Chromosom Cancer 1992; 5: 271–7
  • Ladanyi M, Lewis R, Garin-Chesa P, et al. EWS rearrangement in Ewing's sarcoma and peripheral neuroectodermal tumor. Molecular detection and correlation with cytogenetic analysis and MIC2 expression. Diagn Mol Pathol 1993; 2: 141–6
  • Sorensen P HB, Liu X F, Delattre O, et al. Reverse transcriptase PCR amplification of EWS/FLI-1 fusion transcripts as a diagnostic test for peripheral primitive neuroectodermal tumors of childhood. Diagn Mol Pathol 1993; 2: 147–57
  • Barr F G, Galili N, Holick J, Biegel J A, Rovera G, Emanuel B S. Rearrangement of the PAX3 paired box gene in the paediatric solid tumour alveolar rhabdomyosarcoma. Nat Genet 1993; 3: 113–7

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