Advanced search
Publication Cover
Channels Volume 18, 2024 - Issue 1
Submit an article Journal homepage
847
Views
0
CrossRef citations to date
0
Altmetric
Method

Reducing agents facilitate membrane patch seal integrity and longevity

Damayantee Dasa Dept. of Pharmacology, Alberta Diabetes Institute, University of Alberta, Edmonton, CanadaView further author information
,
Anson Wonga Dept. of Pharmacology, Alberta Diabetes Institute, University of Alberta, Edmonton, CanadaView further author information
,
Timothy N. Friedmanb Neuroscience and Mental Health Institute, University of Alberta, Edmonton, CanadaView further author information
,
Bradley J Kerra Dept. of Pharmacology, Alberta Diabetes Institute, University of Alberta, Edmonton, Canada;b Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Canada;c Department of Anesthesiology and Pain Medicine, University of Alberta, Edmonton, CanadaView further author information
,
Harley T. Kurataa Dept. of Pharmacology, Alberta Diabetes Institute, University of Alberta, Edmonton, Canada;b Neuroscience and Mental Health Institute, University of Alberta, Edmonton, CanadaCorrespondence[email protected]
View further author information
&
Shawn M. Lamothea Dept. of Pharmacology, Alberta Diabetes Institute, University of Alberta, Edmonton, CanadaCorrespondence[email protected]
View further author information
Article: 2297621 | Published online: 28 Dec 2023

References

  • Hamill OP, Marty A, Neher E, et al. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981;391:85–10. doi: 10.1007/BF00656997
  • Neher E, Sakmann B. Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature. 1976;260:799–802. doi: 10.1038/260799a0
  • Sakmann B, Neher E, eds. Single-channel recording. Boston (MA): Springer US; 1995.
  • Hill CL, Stephens GJ. An introduction to patch clamp recording. Methods Mol Biol. 2021;2188:1–19. doi: 10.1007/978-1-0716-0818-0_1
  • Rae JL, Levis RA. Glass technology for patch clamp electrodes. Methods Enzymol. 1992;207:66–92. doi: 10.1016/0076-6879(92)07005-9
  • Priel A, Gil Z, Moy VT, et al. Ionic requirements for membrane-glass adhesion and giga seal formation in patch-clamp recording. Biophys J. 2007;92:3893–3900. doi: 10.1529/biophysj.106.099119
  • Maathuis FJM, Taylor AR, Assmann SM, et al. Seal-promoting solutions and pipette perfusion for patch clamping plant cells. Plant J. 1997;11(4):891–896. doi: 10.1046/j.1365-313X.1997.11040891.x
  • Maguire AD, Plemel JR, Kerr BJ. Enrichment of adult mouse dorsal root ganglia neuron cultures by immunopanning. J Vis Exp. 2023. doi: 10.3791/64603
  • Baronas VA, Yang RY, Kurata HT. Extracellular redox sensitivity of Kv1.2 potassium channels. Sci Rep. 2017;7:9142. doi: 10.1038/s41598-017-08718-z
  • Maki BA, Cummings KA, Paganelli MA, et al. One-channel cell-attached patch-clamp recording. J Vis Exp. 2014. doi: 10.3791/51629
  • Perkins KL. Cell-attached voltage-clamp and current-clamp recording and stimulation techniques in brain slices. J Neurosci Methods. 2006;154:1–18. doi: 10.1016/j.jneumeth.2006.02.010
  • Yan L, Fang Q, Zhang X, et al. Optimal pipette resistance, seal resistance, and zero-current membrane potential for loose patch or breakthrough whole-cell recording in vivo. Front Neural Circuits. 2020;14:34. doi: 10.3389/fncir.2020.00034
  • Roecker AJ, Layton ME, Pero JE, et al. Discovery of arylsulfonamide Nav1.7 inhibitors: IVIVC, MPO methods, and optimization of selectivity profile. ACS Med Chem Lett. 2021;12(6):1038–1049. doi: 10.1021/acsmedchemlett.1c00218
  • Suchyna TM, Markin VS, Sachs F. Biophysics and structure of the patch and the gigaseal. Biophys J. 2009;97:738–747. doi: 10.1016/j.bpj.2009.05.018
  • Corey DP, Stevens CF. Science and technology of patch-recording electrodes. In: Sakmann B Neher E, editors Single-channel recording. Boston (MA): Springer US; 1983. pp. 53–68.
  • Ursell T, Agrawal A, Phillips R. Lipid bilayer mechanics in a pipette with glass-bilayer adhesion. Biophys J. 2011;101(8):1913–1920. doi: 10.1016/j.bpj.2011.08.057
  • Clarke RW. Theory of cell membrane interaction with glass. Phys Rev E. 2021;103:032401. doi: 10.1103/PhysRevE.103.032401
  • Unwin N. Acetylcholine receptor channel imaged in the open state. Nature. 1995;373:37–43. doi: 10.1038/373037a0
  • Baenziger JE, daCosta CJB. Molecular mechanisms of acetylcholine receptor–lipid interactions: from model membranes to human biology. Biophys Rev. 2012;5(1):1–9. doi: 10.1007/s12551-012-0078-7
  • Korade Z, Kenworthy AK. Lipid rafts, cholesterol, and the brain. Neuropharmacology. 2008;55:1265–1273. doi: 10.1016/j.neuropharm.2008.02.019
  • Bradley RP, Radhakrishnan R, Curvature–undulation coupling as a basis for curvature sensing and generation in bilayer membranes, Proceedings of the National Academy of Sciences. 2016;113:E5117–E5124. doi: 10.1073/pnas.1605259113.
  • Alberch L, Yarema KJ. Chapter 3 - bioconjugation reactions in living cells: development, advances, and applications of glycan-specific technologies. In: Karp JM Zhao W, editors Micro- and nanoengineering of the cell surface. Oxford: William Andrew Publishing; 2014. pp. 43–62.
  • Singh R, Whitesides GM. Reagents for rapid reduction of disulfide bonds in proteins. In: Crabb JW, editor Techniques in protein chemistry. Cambridge: Academic Press; 1995. pp. 259–266.
  • Reinhard J, Mattes C, Väth K, et al. A quantitative analysis of cellular lipid compositions during acute proteotoxic ER stress reveals specificity in the production of asymmetric lipids. Front Cell Dev Biol. 2020;8:756. doi: 10.3389/fcell.2020.00756
  • Sinclair J, Olofsson J, Phil J, et al. Stabilization of high-resistance seals in patch-clamp recordings by laminar flow. Anal Chem. 2003;75(23):6718–6722. doi: 10.1021/ac0346611

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.