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Brief Report

Cecum axis (CecAx) preservation reveals physiological and pathological gradients in mouse gastrointestinal epithelium

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Article: 2185029 | Received 16 Nov 2022, Accepted 17 Feb 2023, Published online: 05 Mar 2023

References

  • Burclaff J, Bliton RJ, Breau KA, Ok MT, Gomez-Martinez I, Ranek JS, Bhatt AP, Purvis JE, Woosley JT, Magness ST. A proximal-to-distal survey of healthy adult human small intestine and colon epithelium by single-cell transcriptomics. Cell Mol Gastroenterology Hepatology, 2022;13(5):1554–9. doi:10.1016/j.jcmgh.2022.02.007.
  • Moolenbeek C, Ruitenberg EJ. The ‘Swiss roll’: a simple technique for histological studies of the rodent intestine. Lab Anim, 1980;15(1):57–60. doi:10.1258/002367781780958577.
  • Zaborin A, Krezalek M, Hyoju S, Defazio JR, Setia N, Belogortseva N, Bindokas VP, Guo Q, Zaborina O, Alverdy JC. Critical role of microbiota within cecal crypts on the regenerative capacity of the intestinal epithelium following surgical stress. Am J Physiology Gastroenterology Liver, 2017;312(2):G112–22. doi:10.1152/ajpgi.00294.2016.
  • Zaborin A, Bernabe BP, Keskey R, Sangwan N, Hyoju S, Gottel N, Gilbert JA, Zaborina O, Alverdy JC, Cotter PD Spatial compartmentalization of the microbiome between the lumen and crypts is lost in the murine cecum following the process of surgery, including overnight fasting and exposure to antibiotics. mSystems 2020; 5:e00377–20. 3 10.1128/mSystems.00377-20
  • Snipes RL. Anatomy of the cecum of the laboratory mouse and rat. Anat Embryol (Berl), 1981;162(4):455–474. doi:10.1007/BF00301871.
  • Brown K, Abbott DW, Uwiera RRE, Inglis GD. Removal of the cecum affects intestinal fermentation, enteric bacterial community structure, and acute colitis in mice. Gut Microbes, 2018;9:218–235. doi:10.1080/19490976.2017.1408763.
  • Smith HF, Parker W, Kotzé SH, Laurin M. Morphological evolution of the mammalian cecum and cecal appendix. Cr Palevol, 2017;16(1):39–57. doi:10.1016/j.crpv.2016.06.001.
  • Markham NO, Bloch SC, Shupe JA, Laubacher EN, Thomas AK, Kroh HK, Childress KO, Peritore-Galve FC, Washington MK, Coffey RJ, et al. Murine intrarectal instillation of purified recombinant clostridioides difficile toxins enables mechanistic studies of pathogenesis. Infect Immun, 2021;89(4):89. doi:10.1128/IAI.00543-20.
  • Chen X, Katchar K, Goldsmith JD, Nanthakumar N, Cheknis A, Gerding DN, Kelly CP. A mouse model of clostridium difficile–associated disease. Gastroenterology, 2008;135(6):1984–1992. doi:10.1053/j.gastro.2008.09.002.
  • Hamada H, Hiroi T, Nishiyama Y, Takahashi H, Masunaga Y, Hachimura S, Kaminogawa S, Takahashi-Iwanaga H, Iwanaga T, Kiyono H, et al. Identification of multiple isolated lymphoid follicles on the antimesenteric wall of the mouse small intestine. J Immunol, 2002;168(1):57–64. doi:10.4049/jimmunol.168.1.57.
  • Lin AS, Shuman JHB, Kotnala A, Shaw JA, Beckett AC, Harvey JL, Tuck M, Dixon BREA, Reyzer ML, Algood HMS, et al. Loss of corpus-specific lipids in helicobacter pylori-induced atrophic gastritis. mSphere 2021; 6:e00826–21. 6 10.1128/mSphere.00826-21
  • Liu T, Tang X, Cui Y, Xiong X, Xu Y, Hu S, Feng S, Shao L, Ren Y, Miao H, et al. Fibroblast growth factor 19 improves lps-induced lipid disorder and organ injury by regulating metabolomic characteristics in mice. Oxid Med Cell Longev, 2022;2022:9673512. doi:10.1155/2022/9673512.
  • Romagnolo DF, Donovan MG, Doetschman TC, Oi S. N-6 linoleic acid induces epigenetics alterations associated with colonic inflammation and cancer. Nutrients, 2019;11(1):171. doi:10.3390/nu11010171.
  • Jones BD, Ghori N, Falkow S. Salmonella typhimurium initiates murine infection by penetrating and destroying the specialized epithelial M cells of the peyer’s patches. J Exp Medicine, 1994;180(1):15–23. doi:10.1084/jem.180.1.15.
  • Tahoun A, Mahajan S, Paxton E, Malterer G, Donaldson DS, Wang D, Tan A, Gillespie TL, O’shea M, Roe AJ, et al. Salmonella transforms follicle-associated epithelial cells into m cells to promote intestinal invasion. Cell Host Microbe, 2012;12(5):645–656. doi:10.1016/j.chom.2012.10.009.
  • Collard MK, Bardin J, Laurin M, Ogier-denis E. The cecal appendix is correlated with greater maximal longevity in mammals. J Anat, 2021;239(5):1157–1169. doi:10.1111/joa.13501.
  • Barker N, van EJ, Kuipers J, Kujala P, van den BM, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ, et al. Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature, 2007;449(7165):1003–1007. doi:10.1038/nature06196.
  • von MJ, Ji M, Liang H-E, Locksley RM. Tuft-cell-derived IL-25 regulates an intestinal ILC2–epithelial response circuit. Nature, 2016;529(7585):221–225. doi:10.1038/nature16161.
  • Theriot CM, Koumpouras CC, Carlson PE, Bergin AD II, Young VB. Cefoperazone-treated mice as an experimental platform to assess differential virulence of clostridium difficile strains. Gut Microbes, 2011;2(6):326–334. doi:10.4161/gmic.19142.
  • Shelton CD, Yoo W, Shealy NG, Torres TP, Zieba JK, Calcutt MW, Foegeding NJ, Kim D, Kim J, Ryu S, et al. Salmonella enterica serovar Typhimurium uses anaerobic respiration to overcome propionate-mediated colonization resistance. Cell Rep, 2022;38(1):110180. doi:10.1016/j.celrep.2021.110180.
  • Drewes JL, Chen J, Markham NO, Knippel RJ, Domingue JC, Tam AJ, Chan JL, Kim L, McMann M, Stevens C, et al. Human colon cancer–derived clostridioides difficile strains drive colonic tumorigenesis in mice. Cancer Discov, 2021;12(8):1873–1885. doi:10.1158/2159-8290.CD-21-1273.
  • Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods, 2012;9(7):676–682. doi:10.1038/nmeth.2019.
  • Strohalm M, Hassman M, Košata B, Kodíček M. mMass data miner: an open source alternative for mass spectrometric data analysis. Rapid Commun Mass Spectrom, 2008;22(6):905–908. doi:10.1002/rcm.3444.
  • Wickham H. Ggplot2: elegant graphics for data analysis. New York: Springer-Verlag; 2016.
  • Kassambara AG: “ggplot2” Based Publication Ready Plots. 2020; Available from: https://cran.r-project.org/web/packages/ggpubr/index.html
  • Kassambara A. Rstatix: pipe-friendly framework for basic statistical tests [Internet]. 2020. Available from: https://cran.r-project.org/web/packages/rstatix/index.html
  • Wickham H, Averick M, Bryan J, Chang W, McGowan L, François R, Grolemund G, Hayes A, Henry L, Hester J, et al. Welcome to the Tidyverse. J Open Source Softw, 2019;4(43):1686. doi:10.21105/joss.01686.
  • Team RC. R: a language and environment for ## statistical computing [Internet]. Vienna, Austria: R Foundation for Statistical Computing; 2020. Available fromhttps://www.R-project.org/