Direct evidence of host-mediated glycosylation of NleA and its dependence on interaction with the COPII complex

References

• Havelaar AH, Kirk MD, Torgerson PR, Gibb HJ, Hald T, Lake RJ, Praet N, Bellinger DC, de Silva NR, Gargouri N. et al. World Health Organization global estimates and regional comparisons of the burden of foodborne disease in 2010. PLoS Med. 2015;12(12):e1001923. doi:10.1371/journal.pmed.1001923.
   PubMed Web of Science ®Google Scholar
• Frankel G, Phillips AD, Rosenshine I, Dougan G, Kaper JB, Knutton S. Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements. Mol Microbiol. 1998;30(5):911–6. doi:10.1046/j.1365-2958.1998.01144.x.
   PubMed Web of Science ®Google Scholar
• Moon HW, Whipp SC, Argenzio RA, Levine MM, Giannella RA. Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infect Immun. 1983;41(3):1340–1351. doi:10.1128/iai.41.3.1340-1351.1983.
   PubMed Web of Science ®Google Scholar
• Nataro JP, Kaper JB. Diarrheagenic Escherichia coli. Clin Microbiol Rev. 1998;11(1):142–201. doi:10.1128/cmr.11.1.142.
   PubMed Web of Science ®Google Scholar
• Elliott SJ, Wainwright LA, McDaniel TK, Jarvis KG, Deng YK, Lai LC, McNamara BP, Donnenberg MS, Kaper JB. The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol Microbiol. 1998;28(1):1–4. doi:10.1046/j.1365-2958.1998.00783.x.
   PubMed Web of Science ®Google Scholar
• McDaniel TK, Kaper JB. A cloned pathogenicity island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K-12. Mol Microbiol. 1997;23(2):399–407. doi:10.1046/j.1365-2958.1997.2311591.x.
   PubMed Web of Science ®Google Scholar
• Perna NT, Mayhew GF, Pósfai G, Elliott S, Donnenberg MS, Kaper JB, Blattner FR. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157: H7. Infect Immun. 1998;66(8):3810–3817. doi:10.1128/iai.66.8.3810-3817.1998.
   PubMed Web of Science ®Google Scholar
• Jarvis KG, Girón JA, Jerse AE, Mcdaniel TK, Donnenberg MS, Kaper JB. Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation. Proc Natl Acad Sci U S A. 1995;92(17):7996–8000. doi:10.1073/pnas.92.17.7996.
   PubMed Web of Science ®Google Scholar
• Jarvis KG, Kaper JB. Secretion of extracellular proteins by enterohemorrhagic Escherichia coli via a putative type III secretion system. Infect Immun. 1996;64(11):4826–4829. doi:10.1128/iai.64.11.4826-4829.1996.
   PubMed Web of Science ®Google Scholar
• Gruenheid S, Sekirov I, Thomas NA, Deng W, O’Donnell P, Goode D, Li Y, Frey EA, Brown NF, Metalnikov P. et al. Identification and characterization of NleA, a non-LEE-encoded type III translocated virulence factor of enterohaemorrhagic Escherichia coli O157: H7. Mol Microbiol. 2004;51(5):1233–1249. doi:10.1046/j.1365-2958.2003.03911.x.
   PubMed Web of Science ®Google Scholar
• Coombes BK, Wickham ME, Mascarenhas M, Gruenheid S, Brett Finlay B, Karmali MA. Molecular analysis as an aid to assess the public health risk of non-O157 shiga toxin-producing Escherichia coli strains ‡. Appl Environ Microb. 2008;74(7):2153–2160. doi:10.1128/AEM.02566-07.
   PubMed Web of Science ®Google Scholar
• Mundy R, Jenkins C, Yu J, Smith H, Frankel G. Distribution of espI among clinical enterohaemorrhagic and enteropathogenic Escherichia coli isolates. J Med Microbiol. 2004;53(11):1145–1149. doi:10.1099/JMM.0.45684-0.
   PubMed Web of Science ®Google Scholar
• Mundy R, Petrovska L, Smollett K, Simpson N, Wilson RK, Yu J, Tu X, Rosenshine I, Clare S, Dougan G. et al. Identification of a novel citrobacter rodentium type III secreted protein, EspI, and roles of this and other secreted proteins in infection. Infect Immun. 2004;72(4):2288–2302. doi:10.1128/IAI.72.4.2288-2302.2004.
   PubMed Web of Science ®Google Scholar
• Wickham ME, Brown NF, Boyle EC, Coombes BK, Finlay BB. Virulence is positively selected by transmission success between mammalian hosts. Curr Biol. 2007;17(9):783–788. doi:10.1016/J.CUB.2007.03.067.
   PubMed Web of Science ®Google Scholar
• Burns L, Giannakopoulou N, Zhu L, Xu YZ, Khan RH, Bekal S, Schurr E, Schmeing TM, Gruenheid S. The bacterial virulence factor NleA undergoes host-mediated O-linked glycosylation. Mol Microbiol. 2023;119(2):161–173. doi:10.1111/mmi.14989.
   PubMedGoogle Scholar
• Creuzburg K, Recktenwald J, Kuhle V, Herold S, Hensel M, Schmidt H. The Shiga Toxin 1-converting bacteriophage BP-4795 encodes an NleA-like type III effector protein | enhanced reader. J Bacteriol. 2005;187(24):8494–8498. doi:10.1128/JB.187.24.8494–8498.2005.
   PubMed Web of Science ®Google Scholar
• Lee SF, Kelly M, Mcalister A, Luck SN, Garcia EL, Hall RA, Robins-Browne RM, Frankel G, Hartland EL. A C-terminal class I PDZ binding motif of EspI/NleA modulates the virulence of attaching and effacing Escherichia coli and citrobacter rodentium. Cell Microbiol. 2008;10(2):499–513. doi:10.1111/j.1462-5822.2007.01065.x.
   PubMed Web of Science ®Google Scholar
• Kim J, Thanabalasuriar A, Chaworth-Musters T, Fromme JC, Frey EA, Lario PII, Metalnikov P, Rizg K, Thomas NA, Lee SF. et al. The Bacterial Virulence Factor NleA Inhibits Cellular Protein Secretion by Disrupting Mammalian COPII Function. Cell Host & Microbe. 2007;2(3):160–171. doi:10.1016/j.chom.2007.07.010.
   PubMed Web of Science ®Google Scholar
• Lee MCS, Miller EA, Goldberg J, Orci L, Schekman R. Bi-directional protein transport between the ER and Golgi. Annu Rev Cell Dev Biol. 2004;20(February):87–123. doi:10.1146/annurev.cellbio.20.010403.105307.
   PubMedGoogle Scholar
• Thanabalasuriar A, Bergeron J, Gillingham A, Mimee M, Thomassin JL, Strynadka N, Kim J, Gruenheid S. Sec24 interaction is essential for localization and virulence-associated function of the bacterial effector protein NleA. Cell Microbiol. 2012;14(8):1206–1218. doi:10.1111/j.1462-5822.2012.01789.x.
   PubMed Web of Science ®Google Scholar
• Krieger M, Reddy P, Kozarsky K, Kingsley D, Hobbie L. Chapter 3: analysis of the synthesis, intracellular sorting, and function of glycoproteins using a mammalian cell mutant with reversible glycosylation defects. Methods Cell Biol 1989;32:57–84.
   PubMed Web of Science ®Google Scholar
• Esko JD, Wandall HH, Stanley P. Glycosylation mutants of cultured mammalian cells. Essent Glycobiol. 2022. doi:10.1101/glycobiology.4e.49.
   Google Scholar
• Tollefsen SE, Kornfeld R. Isolation and characterization of lectins from Vicia villosa. Two distinct carbohydrate binding activities are present in seed extracts. J Biol Chem. 1983;258(8):5165–5171. doi:10.1016/S0021-9258(18)32553-5.
   PubMed Web of Science ®Google Scholar
• Bennett EP, Mandel U, Clausen H, Gerken TA, Fritz TA, Tabak LA. Control of mucin-type O-glycosylation: a classification of the polypeptide GalNAc-transferase gene family. Glycobiology. 2012;22(6):736–756. doi:10.1093/glycob/cwr182.
   PubMed Web of Science ®Google Scholar
• Sun X, Zhan M, Sun X, Liu W, Meng X. C1GALT1 in health and disease (review). Oncol Lett. 2021;22(2):1–15. doi:10.3892/ol.2021.12850.
   Web of Science ®Google Scholar
• Wang Y, Ju T, Ding X, Xia B, Wang W, Xia L, He M, Cummings RD. Cosmc is an essential chaperone for correct protein O-glycosylation. Proc Natl Acad Sci U S A. 2010;107(20):9228–9233. doi:10.1073/pnas.0914004107.
   PubMed Web of Science ®Google Scholar
• Sontag RL, Nakayasu ES, Brown RN, Niemann GS, Sydor MA, Sanchez O, Ansong C, Lu S-Y, Choi H, Valleau D. et al. Identification of novel host interactors of effectors secreted by Salmonella and Citrobacter. mSystems. 2016;1(4). doi:10.1128/mSystems.00032-15.
   PubMed Web of Science ®Google Scholar
• Johnson N, Powis K, High S. Post-translational translocation into the endoplasmic reticulum. Biochimica et Biophysica Acta (BBA) - Mol Cell Res. 2013;1833(11):2403–2409. doi:10.1016/j.bbamcr.2012.12.008.
   PubMed Web of Science ®Google Scholar