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Review

Bacterial proteases: current therapeutic use and future prospects for the development of new antibiotics

, &
Pages 221-259 | Published online: 25 Feb 2005

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  • •An interesting paper on the applications of clostripain in peptide/peptide isosteres synthesis.
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  • ••An excellent review on the use of proteases as catalysts forpeptide synthesis.
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  • ••The first report of the 3-dimensional structure of gingipain R(free and inhibited with a chloroketone derivative). This work is of considerable interest for the design of specific and potent inhibitors for these enzymes involved in serious pathologies.
  • POTEMPA J, PIKER, TRAVIS J: Titration and mapping of the active site of cysteine proteinases from Porphyro-monas gingivalis (gin gip ain s) using pep tidyl ch lo r o - m e th an es. Biol. Chem. (1997) 378:223–230.
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  • ••A seminal paper in which bacterial strains defective insortase are described, together with the mechanism by which this protease anchors surface proteins to the cell wall.
  • TON-THAT H, LIU G, MAZMANIAN SK, FAULL KF, SCHNEEWIND 0: Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus, at the LPXTG motif. Proc. Natl. Acad. Sci. USA (1999) 96:12424–12429.
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  • ••A paper in which the first sortase inhibitors are described.
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  • ••In this paper it is clearly proven that bacteria expressingmutant sortase are non-pathogenic and cannot infect their host animals.
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  • ••An excellent and exhaustive review on the mechanisms oftargeting of surface proteins of Gram-positive bacteria.
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  • •An interesting review on zinc enzymes, including some zinc proteases.
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  • ••An exhaustive and excellent review on the Clostridianeurotoxins, by the discoverers of the metalloprotease nature of these toxins.
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  • ••An excellent review on the biochemical mechanismsconnected with the blocking of neurotransmitter release by the botulinum/tetanus MPRs.
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