Capillary microfluidic electrophoretic mobility shift assays: application to enzymatic assays in drug discovery

Bibliography

• Harrison DJ, Fluri K, Seiler K, Micromachining a miniaturized capillary electrophoresis-based chemical analysis system on a chip. Science 1993;261:895-7
   PubMed Web of Science ®Google Scholar
• Lee KJ, Mwongela SM, Kottegoda S, Determination of sphingosine kinase activity for cellular signaling studies. Anal Chem 2008;80:1620-7
   PubMed Web of Science ®Google Scholar
• Wu G, Irvine J, Luft C, Assay development and high-throughput screening of caspases in microfluidic format. Comb Chem High Throughput Screen 2003;6:303-12
   PubMed Web of Science ®Google Scholar
• Perrin D, Frémaux C, Scheer A. Assay development and screening of a serine/threonine kinase in an on-chip mode using Caliper nanofluidics technology. J Biomol Screen 2006;11:359-68
   PubMed Web of Science ®Google Scholar
• Dunne J, Reardon H, Trinh V, Comparison of on-chip and off-chip microfluidic kinase assay formats. Assay Drug Dev Technol 2004;2:121-9
   PubMed Web of Science ®Google Scholar
• Pommereau A, Pap E, Kannt A. Two simple and generic antibody-independent kinase assays : comparison of a bioluminescent and a microfluidic assay format. J Biomol Screen 2004;9:409-16
   PubMed Web of Science ®Google Scholar
• Lin S, Fischl AS, Bi X, Separation of phospholipids in microfluidic chip device: application to high-throughput screening assays for lipid-modifying enzymes. Anal Biochem 2003;314:97-107
   PubMed Web of Science ®Google Scholar
• Perrin D, Frémaux C, Besson D, A microfluidics-based mobiliy shift assay to discover new tyrosine phosphatase inhibitors. J Biomol Screen 2006;11:996-1004
   PubMed Web of Science ®Google Scholar
• Beck ZQ, Hervio L, Dawson PE, Identification of efficiently cleaved substrates for HIV-1 protease using a phage display library and use in inhibitor development. Virology 2000;274:391-401
   PubMed Web of Science ®Google Scholar
• Gosalia DN, Salisbury CM, Ellman JA, Diamond SL. High throughput substrate specificity profiling of serine and cysteine proteases using solution-phase fluorogenic peptide microarrays. Mol Cell Proteomics 2005:4;626-36
   PubMed Web of Science ®Google Scholar
• Liu Y, Gerber R, Wu J, High-throughput assays for sirtuin enzymes: a microfluidic mobility shift assay and a bioluminescence assay. Anal Biochem 2008;378:53-9
   PubMed Web of Science ®Google Scholar
• Butler KV, He R, McLaughlin K, Stereoselective HDAC inhibition from cysteine-derived zinc-binding groups. Chem Med Chem 2009;4:1292-301
   Web of Science ®Google Scholar
• Blackwell L, Norris J, Suto C, Janzen WP. The use of diversity profiling to characterize chemical modulators of the histone deacetylases. Life Sci 2008;82:1050-8
   PubMed Web of Science ®Google Scholar
• Liu Y, Gerber R, Wu J, High-throughput assays for sirtuin enzymes: a microfluidic mobility shift assay and a bioluminescence assay. Anal Biochem 2008;378:53-9
   PubMed Web of Science ®Google Scholar
• Foulds GJ, Etzkorn FA. A capillary electrophoresis mobility shift assay for protein-DNA binding affinities free in solution. Nucleic Acids Res 1998;26:4304-5
   PubMed Web of Science ®Google Scholar
• Chuang YJ, Huang JW, Makamba H, Electrophoretic mobility shift assay on poly(ethylene glycol)-modified glass microchips for the study of estrogen responsive element binding. Electrophoresis 2006;27:4158-65
   PubMed Web of Science ®Google Scholar
• Perrin D, Martin T, Cambet Y, Overcoming the hurdle of fluorescent compounds in kinase screening: a case study. Assay Drug Dev Technol 2004;4:185-96
   Web of Science ®Google Scholar
• Jia Y, Gu X, Brinker A, Measuring the tyrosine kinase activity: a review of biochemical and cellular assay technologies. Expert Opin Drug Discov 2008;3:959-78
   PubMed Web of Science ®Google Scholar
• Antonsson B, Marshall CJ, Montessuit S, An in vitro 96-well plate assay of the mitogen-activated protein kinase cascade. Anal Biochem 1999;267:294-9
   PubMed Web of Science ®Google Scholar
• Mitogen activated protein kinase cascade. Caliper LifeSciences application Note 2007. Available from: http://caliperls.com/assets/009/5901.pdf
   Google Scholar
• “LabChip mobility-shift assay: phosphotidylinositol-3 kinase PI3Ka” application note 2008 Available from: http://caliperls.com/assets/013/6998.pdf
   Google Scholar
• “Sphingosine kinase 1 and Sphingosine kinase 2 assays” application note 2006. Available from: http://caliperls.com/assets/001/5897.pdf
   Google Scholar
• Alonso A, Sasin J, Bottini N, Protein Tyrosine Phosphatases in the Human Genome. Cell 2004;117:699-711
   PubMed Web of Science ®Google Scholar
• Perrin D, Hooft van Huijsduijnen R. An introduction to the protein tyrosine phosphatase gene family and screening assay development. In: Minor L, editor, Handbook of assay development in drug development, CRC Press: Boca Raton, FL; 2006. p. 7-23
   Google Scholar
• Rowe T, Hale C, Zhou A, A high-throughput microfluidic assay for SH2 domain-containing inositol 5-phosphatase 2. Assay Drug Dev Technol 2006;4:175-83
   PubMed Web of Science ®Google Scholar
• Lipinski CA, Lombardo F, Dominy BW, Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. Adv Drug Delivery Rev 2001;46:3-26
   PubMed Web of Science ®Google Scholar
• “HDAC assays on LabChip” application note 2008 Available from: http://caliperls.com/assets/021/8177.pdf
   Google Scholar
• Hoffmann K, Heltweg B, Jung M. Improvement and validation of the fluorescence-based histone deacetylase assay using an internal standard. Arch Pharm (Weinheim) 2001;334:248-52
   PubMed Web of Science ®Google Scholar
• “NAD+ dependent protein deacetylase (Sirtuins, Class III) assay” application note 2006 Available from: http://caliperls.com/assets/009/5900.pdf
   Google Scholar
• Cosgrove MS, Bever K, Avalos JL, The structural basis of sirtuin substrate affinity. Biochemistry 2006;45:7511-21
   PubMedGoogle Scholar
• Kim JH, Shin HJ, Cho H, A microfluidic protease activity assay based on the detection of fluorescence polarization. Anal Chim Acta 2006;577:171-7
   PubMed Web of Science ®Google Scholar
• Turk BE. Mixture-based peptide libraries for identifying protease cleavage motifs. Methods Mol Biol 2009;539:79-91
   PubMedGoogle Scholar
• “MMP9: matrix metalloproteinase 9 assay” application note 2005 Available from: http://caliperls.com/assets/008/5895.pdf
   Google Scholar
• “LabChip mobility-shift assay: phosphodiesterases” application note 2008. Available from: http://caliperls.com/assets/016/7402.pdf
   Google Scholar
• Yang Q, Zhao YC, Xiong Q, Cheng J. Rapid chip-based capillary electrophoretic mobility shift assay with negative pressure injection for the binding study of transcription factor Abf1 in Saccharomyces cerevisiae. Electrophoresis 2008;29(24):5003-9
   PubMed Web of Science ®Google Scholar
• Li C, Martin LM. A robust method for determining DNA binding constants using capillary zone electrophoresis. Anal Biochem 1998;263:72-8
   PubMed Web of Science ®Google Scholar
• Sobczak K, Krzyzosiak WJ. RNA structure analysis assisted by capillary electrophoresis. Nucleic Acids Res 2002;30:e124
   PubMed Web of Science ®Google Scholar
• Nishikawa F, Murakami K, Noda K, Detection of structural changes of RNA aptamer containing GGA repeats under the ionic condition using the microchip electrophoresis. Nucleic Acids Symp Ser (Oxf). 2007;(51)397-8
   PubMedGoogle Scholar
• Mucha P, Szyk A, Rekowski P, Interaction of RNA with phage display selected peptides analyzed by capillary electrophoresis mobility shift assay. RNA 2002;8:698-704
   PubMed Web of Science ®Google Scholar
• Mendonsa SD, Bowser MT. In vitro selection of aptamers with affinity for neuropeptide Y using capillary electrophoresis. J Am Chem Soc 2005;127:9382-3
   PubMed Web of Science ®Google Scholar
• Huang CC, Cao Z, Chang HT, Tan W. Protein-protein interaction studies based on molecular aptamers by affinity capillary electrophoresis. Anal Chem 2004;76:6973-81
   PubMed Web of Science ®Google Scholar
• Cunliffe JM, Sunahara RK, Kennedy RT. Detection of g protein coupled receptor mediated adenylyl cyclase activity by capillary electrophoresis using fluorescently labeled ATP. Anal Chem 2007;79:7534-9
   PubMed Web of Science ®Google Scholar
• Available from: www.spinx-technologies.com
   Google Scholar
• Wu J, Barbero R, Vajjhala S, Real-time analysis of enzyme kinetics via micro parallel liquid chromatography. Assay Drug Dev Technol 2006;4:653-60
   PubMed Web of Science ®Google Scholar
• Wu J, Vajjhala S, O'Connor S. A microPLC-based approach for determining kinase-substrate specificity. Assay Drug Dev Technol 2007;5:559-66
   PubMed Web of Science ®Google Scholar
• Tran L, Farinas J, Ruslim-Litrus L, Agonist-induced calcium response in single human platelets assayed in a microfluidic device. Anal Biochem 2005;341(2):361-8
   PubMed Web of Science ®Google Scholar
• Dittrich P, Manz A. Lab-on-a-chip: microfluidics in drug discovery. Nat Rev Drug Discov 2006;5:210-18
   PubMed Web of Science ®Google Scholar
• Chao P, Maguire T, Novik E, Evaluation of a microfluidic based cell culture platform with primary human hepatocytes for the prediction of hepatic clearance in human. Biochem Pharmacol 2009;78:625-32
   PubMed Web of Science ®Google Scholar
• Gómez-Sjöberg R, Leyrat A, Pirone D, Versatile, fully automated, microfluidic cell culture system. Anal Chem 2007;79:8557-63
   PubMed Web of Science ®Google Scholar