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Ligand supplementation as a method to increase soluble heterologous protein production

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Pages 137-143 | Published online: 09 Jan 2014

References

  • Hunt I. From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein Expr. Purif.40(1), 1–22 (2005).
  • Makrides SC. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev.60(3), 512–538 (1996).
  • Structural Genomics Consortium A, Berkeley Structural Genomics Center, China Structural Genomics Consortium et al. Guidelines for the production and purification of recombinant proteins in E. coli. Nat. Methods (2008) (In press).
  • Wu X, Jornvall H, Berndt KD, Oppermann U. Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance. Biochem. Biophys. Res. Commun.313(1), 89–96 (2004).
  • Wynn RM, Davie JR, Song JL, Chuang JL, Chuang DT. Expression of E1 component of human branched-chain a-keto acid dehydrogenase complex in Escherichia coli by cotransformation with chaperonins GroEL and GroES. Meth. Enzymol.324, 179–191 (2000).
  • Ailor E, Betenbaugh MJ. Overexpression of a cytosolic chaperone to improve solubility and secretion of a recombinant IgG protein in insect cells. Biotechnol. Bioeng.58(2–3), 196–203 (1998).
  • Widersten M. Heterologous expression in Escherichia coli of soluble active-site random mutants of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 by coexpression of molecular chaperonins GroEL/ES. Protein Expr. Purif.13(3), 389–395 (1998).
  • Gileadi O, Knapp S, Lee WH et al. The scientific impact of the Structural Genomics Consortium: a protein family and ligand-centered approach to medically-relevant human proteins. J. Struct. Funct. Genomics8(2–3), 107–119 (2007).
  • Niesen FH, Berglund H, Vedadi M. The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability. Nat. Protoc.2(9), 2212–2221 (2007).
  • Vedadi M, Niesen FH, Allali-Hassani A et al. Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination. Proc. Natl Acad. Sci. USA103(43), 15835–15840 (2006).
  • Elleby B, Svensson S, Wu X et al. High-level production and optimization of monodispersity of 11β-hydroxysteroid dehydrogenase type 1. Biochim. Biophys. Acta1700(2), 199–207 (2004).
  • Hult M, Shafqat N, Elleby B et al. Active site variability of type 1 11β-hydroxysteroid dehydrogenase revealed by selective inhibitors and cross-species comparisons. Mol. Cell. Endocrinol.248(1–2), 26–33 (2006).
  • Shafqat N, Elleby B, Svensson S et al. Comparative enzymology of 11β-hydroxysteroid dehydrogenase type 1 from glucocorticoid resistant (Guinea pig) versus sensitive (human) species. J. Biol. Chem.278(3), 2030–2035 (2003).
  • Kallberg Y, Oppermann U, Jornvall H, Persson B. Short-chain dehydrogenase/reductase (SDR) relationships: a large family with eight clusters common to human, animal, and plant genomes. Protein Sci.11(3), 636–641 (2002).
  • Oppermann U, Filling C, Hult M et al. Short-chain dehydrogenases/reductases (SDR): the 2002 update. Chem. Biol. Interact.143–144, 247–253 (2003).
  • Lukacik P, Kavanagh KL, Oppermann U. Structure and function of human 17b-hydroxysteroid dehydrogenases. Mol. Cell. Endocrinol.248(1–2), 61–71 (2006).
  • Wu X, Lukacik P, Kavanagh KL, Oppermann U. SDR-type human hydroxysteroid dehydrogenases involved in steroid hormone activation. Mol. Cell. Endocrinol.265–266, 71–76 (2007).
  • Su X, Vicker N, Ganeshapillai D et al. Benzothiazole derivatives as novel inhibitors of human 11β-hydroxysteroid dehydrogenase type 1. Mol. Cell. Endocrinol.248(1–2), 214–217 (2006).
  • Vicker N, Su X, Ganeshapillai D et al. Novel non-steroidal inhibitors of human 11β-hydroxysteroid dehydrogenase type 1. J. Steroid Biochem. Mol. Biol.104(3–5), 123–129 (2007).
  • Olson S, Aster SD, Brown K et al. Adamantyl triazoles as selective inhibitors of 11β-hydroxysteroid dehydrogenase type 1. Bioorg. Med. Chem. Lett.15(19), 4359–4362 (2005).
  • Strauss A, Fendrich G, Horisberger MA et al. Improved expression of kinases in Baculovirus-infected insect cells upon addition of specific kinase inhibitors to the culture helpful for structural studies. Protein Expr. Purif.56(2), 167–176 (2007).
  • Pyle LE, Barton P, Fujiwara Y, Mitchell A, Fidge N. Secretion of biologically active human proapolipoprotein A-I in a baculovirus-insect cell system: protection from degradation by protease inhibitors. J. Lipid Res.36(11), 2355–2361 (1995).
  • Hassell AM, An G, Bledsoe RK et al. Crystallization of protein–ligand complexes. Acta Crystallogr. D Biol. Crystallogr.63(Pt 1), 72–79 (2007).
  • Szczebara FM, Chandelier C, Villeret C et al. Total biosynthesis of hydrocortisone from a simple carbon source in yeast. Nat. Biotechnol.21(2), 143–149 (2003).
  • Waldo GS, Standish BM, Berendzen J, Terwilliger TC. Rapid protein-folding assay using green fluorescent protein. Nat. Biotechnol.17(7), 691–695 (1999).
  • Yang JK, Park MS, Waldo GS, Suh SW. Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis.Proc. Natl Acad. Sci. USA100(2), 455–460 (2003).
  • Inglese J, Auld DS, Jadhav A et al. Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries. Proc. Natl Acad. Sci. USA103(31), 11473–11478 (2006).

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