127
Views
31
CrossRef citations to date
0
Altmetric
Original Article

Effects of different density gradient separation techniques on neutrophil function

, , , &
Pages 385-391 | Received 04 Oct 1993, Accepted 18 Mar 1994, Published online: 08 Jul 2009

References

  • Böyum A. Isolation of mononuclear cells and granulocytes from human blood: isolation of mononuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 G. Scand J Clin Lab Invest 1968; 21(suppl. 97)77–89
  • Jepsen L V, Skottun T. A rapid one-step method for the isolation of human granulocytes from whole blood. Scand J Clin Lab Invest 1982; 42: 235–38
  • Harbeck R J, Hoffman A A, Redecker S, Biundo T, Kurnick J. The isolation and functional activity of polymorphonuclear leukocytes and lymphocytes separated from whole blood on a single percoll density gradient. Clin Immunol Immunopath 1982; 23: 682–90
  • Roberts R L, Mounessa N L, Gallin J I. Increasing extracellular potassium causes calcium-dependent shape change and facilitates concanavalin A capping in human neutrophils. J Immunol 1984; 132: 2000–6
  • Bignold L P, Ferrante A. Mechanism of separation of polymorphonuclear leukocytes from whole blood by the one-step Hypaque-Ficoll method. J Immunol Methods 1987; 96: 29–33
  • Keller H U, Naef A, Zimmermann A. Effects of colchicine vinblastine and nocodazole on polarity, motility, chemotaxis and cAMP levels of human polymorphonuclear leukocytes. Exp Cell Res 1984; 153: 173–85
  • Haslett C, Guthrie L A, Kopaniak M M, Johnston R B, Jr, Henson P M. Modulation of multiple neutrophil functions by preparative methods or trace concentrations of bacterial lipopolysac-charide. Am J Pathol 1985; 119: 101–10
  • Seligmann B E, Gallin J I. Use of lipophilic probes of membrane potential to assess human neutrophil activation. Abnormality in chronic granulomatous disease. J Clin Invest 1980; 66: 493–503
  • Seligmann B E, Gallin E K, Martin D L, Shain W, Gallin J I. Interaction of chemotactic factors with human polymorphonuclear leukocytes: studies using a membrane potential-sensitive cyanine dye. J Membr Biol 1980; 52: 257–72
  • Haston W S, Shields J M. Neutrophil leucocyte chemotaxis: a simplified assay for measuring polarising responses to chemotactic factors. J Immunol Methods 1985; 81: 229–37
  • Needham P L. Separation of human blood using ‘Mono-Poly Resolving Medium’. J Immunol Methods 1987; 99: 283–4
  • Babior B M. The respiratory burst oxidase. Hematol Oncol Clin North Am 1988; 2: 201–12
  • Sadler K L, Badwey J A. Second messengers involved in superoxide production by neutrophils: function and metabolism. Hematol Oncol Clin North Am 1988; 2: 185–200
  • Grisham M B, Engerson T D, McCord J M, Jones H P. A comparative study of neutrophil purification and function. J Immunol Methods 1982; 82: 315–20
  • Brattig N W, Medina-De la Garza C E, Tischendorf F W. Comparative study of eosinophil purification on Nycodenz, Metrizamide and Percoll density gradients. Eur J Haematol 1987; 39: 148–53
  • Eggleton P, Gargan R, Fisher D. Rapid method for the isolation of neutrophils in high yield without the use of dextran or density gradient polymers. J Immunol Methods 1989; 121: 105–13

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.