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Gastrointestinal Physiology

In vitro assessment of epithelial electrical resistance in human esophageal and jejunal mucosae and in Caco-2 cell layers

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Pages 1321-1333 | Received 13 Aug 2012, Accepted 15 Aug 2012, Published online: 25 Sep 2012

References

  • Barlow WJ, Orlando RC. The pathogenesis of heartburn in nonerosive reflux disease: a unifying hypothesis. Gastroenterology 2005;128:771–8.
  • Turner JR. Intestinal mucosal barrier function in health and disease. Nat Rev Immunol 2009;9:799–809.
  • Catalioto RM, Maggi CA, Giuliani S. Intestinal epithelial barrier dysfunction in disease and possible therapeutical interventions. Curr Med Chem 2011;18:398–426.
  • Lam YY, Mitchell AJ, Holmes AJ, Denyer GS, Gummesson A, Caterson ID, Role of the gut in visceral fat inflammation and metabolic disorders. Obesity (Silver Spring) 2011;19:2113–20.
  • Anderson JM, Van Itallie CM. Physiology and function of the tight junction. Cold Spring Harb Perspect Biol 2009;1:a002584.
  • Gitter AH, Fromm M, Schulzke JD. Impedance analysis for the determination of epithelial and subepithelial resistance in intestinal tissues. J Biochem Biophys Methods 1998;37:35–46.
  • Fromm M, Krug SM, Zeissig S, Richter JF, Rosenthal R, Schulzke JD, High-resolution analysis of barrier function. Ann NY Acad Sci 2009;1165:74–81.
  • Reims A, Strandvik B, Sjovall H. Epithelial electrical resistance as a measure of permeability changes in pediatric duodenal biopsies. J Pediatr Gastroenterol Nutr 2006;43:619–23.
  • Hemlin M, Jodal M, Lundgren O, Sjovall H, Stage L. The importance of the subepithelial resistance for the electrical properties of the rat jejunum in vitro. Acta Physiol Scand 1988;134:79–88.
  • Zucco F, Batto AF, Bises G, Chambaz J, Chiusolo A, Consalvo R, An inter-laboratory study to evaluate the effects of medium composition on the differentiation and barrier function of Caco-2 cell lines. Altern Lab Anim 2005;33:603–18.
  • Beaslas O, Torreilles F, Casellas P, Simon D, Fabre G, Lacasa M, Transcriptome response of enterocytes to dietary lipids: impact on cell architecture, signaling, and metabolism genes. Am J Physiol Gastrointest Liver Physiol 2008;295:G942–52.
  • Fromm M, Schulzke JD, Hegel U. Epithelial and subepithelial contributions to transmural electrical resistance of intact rat jejunum, in vitro. Pflugers Arch 1985;405:400–2.
  • Yotsuyanagi T, Molokhia A, Hwang S, Ho NF, Flynn GL, Higuchi WI. Systems approach to vaginal delivery of drugs I: development of in situ vaginal drug absorption procedure. J Pharm Sci 1975;64:71–6.
  • Nejdfors P, Ekelund M, Jeppsson B, Westrom BR. Mucosal in vitro permeability in the intestinal tract of the pig, the rat, and man: species- and region-related differences. Scand J Gastroenterol 2000;35:501–7.
  • Baddeley AJ, Gundersen HJ, Cruz-Orive LM. Estimation of surface area from vertical sections. J Microsc 1986;142:259–76.
  • Krug SM, Fromm M, Gunzel D. Two-path impedance spectroscopy for measuring paracellular and transcellular epithelial resistance. Biophys J 2009;97:2202–11.
  • Suzuki K, Kottra G, Kampmann L, Fromter E. Square wave pulse analysis of cellular and paracellular conductance pathways in Necturus gallbladder epithelium. Pflugers Arch 1982;394:302–12.
  • Sambuy Y, De Angelis I, Ranaldi G, Scarino ML, Stammati A, Zucco F. The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biol Toxicol 2005;21:1–26.
  • Borchardt RT, Hidalgo IJ, Raub TJ, Borchardt RT. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability, Gastroenterology, 96, 736-749, 1989–the backstory. AAPS J 2011;13:323–7.
  • Artursson P. Epithelial transport of drugs in cell culture. I: a model for studying the passive diffusion of drugs over intestinal absorptive (Caco-2) cells. J Pharm Sci 1990;79:476–82.
  • Muendoerfer M, Schaefer UF, Koenig P, Walk JS, Loos P, Balbach S, Online monitoring of transepithelial electrical resistance (TEER) in an apparatus for combined dissolution and permeation testing. Int J Pharm 2010;392:134–40.
  • Yu H, Sinko PJ. Influence of the microporous substratum and hydrodynamics on resistances to drug transport in cell culture systems: calculation of intrinsic transport parameters. J Pharm Sci 1997;86:1448–57.
  • Behrens I, Kissel T. Do cell culture conditions influence the carrier-mediated transport of peptides in Caco-2 cell monolayers? Eur J Pharm Sci 2003;19:433–42.
  • Xu DZ, Lu Q, Kubicka R, Deitch EA. The effect of hypoxia/reoxygenation on the cellular function of intestinal epithelial cells. J Trauma 1999;46:280–5.
  • Tappenden KA. Provision of phosphorylatable substrate during hypoxia decreases jejunal barrier function. Nutrition 2002;18:168–72.
  • Bouvry D, Planes C, Malbert-Colas L, Escabasse V, Clerici C. Hypoxia-induced cytoskeleton disruption in alveolar epithelial cells. Am J Respir Cell Mol Biol 2006;35:519–27.
  • Farre R, van Malenstein H, De Vos R, Geboes K, Depoortere I, Vanden Berghe P, Short exposure of oesophageal mucosa to bile acids, both in acidic and weakly acidic conditions, can impair mucosal integrity and provoke dilated intercellular spaces. Gut 2008;57:1366–74.
  • Byrne AM, Sharma R, Duggan G, Kelleher D, Long A. Deoxycholic acid impairs glycosylation and fucosylation processes in esophageal epithelial cells. Glycobiology 2012;22:638–48.
  • Goldstein JL, Schmidt LN, Al-Bazzaz FJ, Layden TJ. Effects of taurine-conjugated bile salts on ionic transport of the rabbit esophageal mucosa. Am J Physiol 1986;251:G688–94.
  • Salo JA, Lehto VP, Kivilaakso E. Morphological alterations in experimental esophagitis. Light microscopic and scanning and transmission electron microscopic study. Dig Dis Sci 1983;28:440–8.
  • Salo JA, Lehto VP, Myllarniemi HS, Kivilaakso EO. Morphological alterations in tryptic esophagitis: an experimental light microscopic and scanning and transmission electron microscopic study in rabbits. J Surg Res 1990;49:14–17.
  • Theisen J, Nehra D, Citron D, Johansson J, Hagen JA, Crookes PF, Suppression of gastric acid secretion in patients with gastroesophageal reflux disease results in gastric bacterial overgrowth and deconjugation of bile acids. J Gastrointest Surg 2000;4:50–4.
  • Reims A, Redfors S, Sjovall H, Strandvik B. Cysteinyl leukotrienes are secretagogues in atrophic coeliac and in normal duodenal mucosa of children. Scand J Gastroenterol 2005;40:160–8.
  • Schulzke JD, Schulzke I, Fromm M, Riecken EO. Epithelial barrier and ion transport in coeliac sprue: electrical measurements on intestinal aspiration biopsy specimens. Gut 1995;37:777–82.
  • Casselbrant A, Edebo A, Hallersund P, Spak E, Helander HF, Jonson C, Angiotensin II receptors are expressed and functional in human esophageal mucosa. Am J Physiol Gastrointest Liver Physiol 2009;297:G1019–27.
  • Gitter AH, Bertog M, Schulzke J, Fromm M. Measurement of paracellular epithelial conductivity by conductance scanning. Pflugers Arch 1997;434:830–40.
  • Jovov B, Que J, Tobey NA, Djukic Z, Hogan BL, Orlando RC. Role of E-cadherin in the pathogenesis of gastroesophageal reflux disease. Am J Gastroenterol 2011;106:1039–47.

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