Publication Cover
Archives of Andrology
Journal of Reproductive Systems
Volume 27, 1991 - Issue 3
84
Views
4
CrossRef citations to date
0
Altmetric
Original Article

Recovery of Motile Sperm Using the Osmolality-Gradient Technique

, , &
Pages 177-184 | Received 29 May 1991, Accepted 07 Jun 1991, Published online: 09 Jul 2009

References

  • Broer K H, Dauber U. A filtering method for cleaning up spermatozoa in cases of asthenospermia. Int J Fertil 1978; 23: 234–237
  • Casslien B, Nilsson B. Human uterine fluid examined in undiluted samples for osmolarity and the concentrations of inorganic ions, albumin, glucose, and urea. Am J Obstet Gynecol 1984; 150: 877–881
  • Ericsson R J. Isolation and storage of progressively motile human sperm. Andrologia 1979; 9: 111–114
  • Forster M S, Smith W D, Lee W I, Berger R E, Karp L E, Stenehever M A. Selection of human spermatozoa according to their relative motility and their interaction with zona-free hamster eggs. Fertil Steril 1983; 40: 655–660
  • Gorus F K, Pipeleers D G. A rapid method for fractionation of human spermatozoa according to their progressive motility. Fertil Steril 1981; 35: 662–665
  • Holt W V, Morre H DM, Hillier S G. Computer-assisted measurement of sperm swimming speed in human semen: correlation of results with in vitro fertilization assays. Fertil Steril 1985; 44: 112–119
  • Jeyendran R S, Perez-Pelaez M, Crabo B G. Concentration of viable spermatozoa for artificial insemination. Fertil Steril 1986; 45: 132–134
  • Jones R C, Holt W V. The effect of washing on the ultrastructure and cytochemistry of ram spermatozoa. J Reprod Fertil 1974; 41: 159–167
  • Koper A, Evans P R, Witherow R ON, Flynn J T, Bayliss M, Blandy J P. A technique for selecting and concentrating the motile sperm from semen in oligozoospermia. Br J Urol 1979; 51: 587–590
  • Lopata A, Patullo M J, Chang A, James B. A method for collecting motile spermatozoa from human semen. Fertil Steril 1976; 27: 677–684
  • Lucena E, Lucena C, Gomez M, Ortiz J A, Ruiz J, Arango A, Diaz C, Beuerman C. Recovery of motile sperm using the migration-sedimentation technique in an in vitro fertilization-embryo transfer programme. Hum Reprod 1989; 4: 163–165
  • Mahadevan M M, Trounson A O. The influence of seminal characteristics on the success rate of human in vitro fertilization. Fertil Steril 1984; 42: 400–405
  • Makler A, David R, Blumenfeld Z, Better O S. Factors affecting sperm motility, VII: sperm viability as affected by change of pH and osmolarity of semen and urine specimens. Fertil Steril 1981; 36: 507–511
  • Miyamato H, Chang M C. The importance of serum albumin and metabolic intermediates for capacitation of spermatozoa and fertilization of mouse eggs in vitro. J Reprod Fertil 1973; 32: 193–205
  • Paulson J D, Polakoski K L. A glass wool column procedure for removing extraneous material from the human ejaculate. Fertil Steril 1977; 28: 178–181
  • Quinn P, Kerin J F, Warnes G M. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil Steril 1985; 44: 493–498
  • Tatsumi K, Noda Y, Matsumoto H, Umaoka Y, Yano J, Fukuda A. AIH using the sperm collected by the “migration-gravity sedimentation” method. Jpn J Fertil Steril 1988; 33: 645–650
  • Van der Ven H H, Jeyendran R S, Tunnerhoff A, Hoebbel K, Al-Hassani S, Diedrich K, Krebs D, Perez-Pelaez M. Glass wool column filtration of human semen: relation to swim up procedure and IVF outcome. Hum Reprod 1988; 3: 85–88

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.