Characterization of a Δ⁵-3β-Hydroxy Pathway of Testosterone Synthesis in the Human Spermatozoa

References

• Briggs M. Tetracycline and steroid hormone binding to human spermatozoa. Acta Endocrinol 1974; 75: 785–792
   PubMedGoogle Scholar
• Castaneda E, Rios E P, Perez A E, Lichtenberg R, Cordero C, Iramain C A, Perez-Palacios G. In vitro transformation of steroid hormones by human semen. Fertil Steril 1974; 25: 261–270
   PubMed Web of Science ®Google Scholar
• Cheng C Y, Rose R J, Boettcher B. The binding of oestradiol-17p to human spermatozoa: an electron microscope autoradiographic study. Int J Androl 1981; 4: 304–313
   PubMedGoogle Scholar
• Chew P CT, Loganath A, Peh K L, Chow W P, Gunasegaram R, Ratnam S S. Concentrations of intracellular sex steroids in human spermatozoa. Arch Androl 1993; 30: 165–170
   PubMed Web of Science ®Google Scholar
• Go K J, Wolf D P. The role of sterols in sperm capacitation. Adv Lipid Res 1983; 20: 317–330
   PubMedGoogle Scholar
• Gunasegaram R, Loganath A, Peh K L, Chow W P, Chew P CT, Ratnam S S (1991) Ability of humam spermatozoa to utilize 1,2,6,7-[3H]dehydroepiandrosterone for [3H]testosterone synthesis in vitro. 13th World Congress of Gynaecology and Obstetrics, Singapore, September, 15–201991, 209, Abstr.
   Google Scholar
• Gunasegaram R, Chew P CT, Loganath A, Peh K L, Chow W P, Ratnam S S (1994) Characterization of a Δ4-3-keto pathway for testosterone synthesis in the human spermatozoa. 14th World Congress of Gynaecology and Obstetrics, MontrealCanada, September, 24–301994, 12, Abstr.
   Google Scholar
• Gunasegaram R, Peh K L, Loganath A, Chew P CT, Ratnam S S. In vitro formation of [14C] cholesterol from 2-[14C]acetate in human spermatozoa. Med Sci Res 1995; 23: 315–316
   Google Scholar
• Kerr J B, Miller M, Maddocks S, Sharpe R M. Stage dependent changes in spermatogenesis and Sertoli cells in relation to the onset of spermatogenesis failure following withdrawal of testosterone. Anat Rec 1993; 235: 547–559
   PubMedGoogle Scholar
• Kessler M J. Liquid Scintillation Analysis, Science and Technology. Packard Instrument, Meriden, CT 1989; 5.1–5.18
   Google Scholar
• Loganath A, Gunasegaram R, Peh K L, Chow W P, Chew P CT, Ratnam S S. In vitro formation of testosterone via the A5-pathway in the human umbilical cord. Steroids 1992; 57: 189–192
   PubMed Web of Science ®Google Scholar
• Loganath A, Gunasegaram R, Peh K L, Chow W P, Chew P CT, Ratnam S S. A relationship between sperm count and intracellular progesterone, 17α-hydroxyprogesterone and Cortisol concentrations in human spermatozoa. Fertil Steril 1994; 61: 990–992
   PubMed Web of Science ®Google Scholar
• Paulson J D, Polakoski K L. A glass wool column procedure for removing extraneous material from the human ejaculate. Fertil Steril 1977; 28: 178–181
   PubMed Web of Science ®Google Scholar
• Rajalakshmi M, Ramakrishnan P R, Kapur M M, Sharma R S, Mokkapati S. Steroid metabolism by germ cells and spermatozoa in men after vasoepididymostomy. Steroids 1991; 56: 527–532
   PubMed Web of Science ®Google Scholar
• Rajalakshmi M, Sehgal A, Pruthi J S, Anand Kumar T C. Steroid metabolism by monkey and human spermatozoa. Steroids 1983; 41: 587–595
   PubMed Web of Science ®Google Scholar
• Steinberger E, Fischer M, Smith K D. Relation of in vitro metabolism of steroids in human testicular tissue to histologic and clinical findings. The Human Testis, E Rosenberg, C A Paulsen. Plenum, New York 1970; 439–458
   Google Scholar
• Sun Y-T, Wreford N G, Robertson D M, de Kretser D M. Quantitative cytological studies of spermatogenesis in intact and hypophysectomised rats: identification of androgen-dependent steps. Endocrinology 1990; 127: 1215–1223
   PubMed Web of Science ®Google Scholar
• Vornberger W, Prins G, Musto N A, Suarez-Quian C A. Androgen receptor distribution in rat testis: new implications for androgen regulation of spermatogenesis. Endocrinology 1994; 134: 2307–2316
   PubMed Web of Science ®Google Scholar
• World Health Organization. Laboratory Manual for the Examination of Human Semen and Semen Cervical Mucus Interaction, 1992. Cambridge University Press, CambridgeUK 1992
   Google Scholar
• Wright W M, Frankel A I. An androgen receptor in the nuclei of late spermatids in testes of male rats. Endocrinology 1980; 107: 314–318
   PubMed Web of Science ®Google Scholar