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Original Article

Acridine orange differential staining of total DNA and RNA in normal and galactosemic lens epithelial cells in culture using flow cytometry

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Pages 269-273 | Received 09 May 1994, Accepted 21 Dec 1994, Published online: 02 Jul 2009

References

  • Fagerholm P., Philipson B. Human len epithelium in normal and cataractous lenses. Invest. Ophth. Vis. Sci. 1981; 21: 408–414
  • Kobayashi Y., Suzuki T. The aging lens: Ultrastructure changes in cataract. Cataract and Abnormalities of the Lens., J. G. Bellows. Greene and Stratton, Inc, New York 1975; 325–330
  • Eshaghian J., Streeten B. Human posterior subcapsular cataract. An ultrastructural study of the posterior migrating cells. Arch. Ophth. 1980; 98: 134–140
  • Francois J., Victoria-Troncoso V. Histology of the epithelium of the normal and cataractous lens. Ophthalmologica 1978; 177: 168–175
  • Kinoshita J. Mechanisms initiating cataract formation (Proctor Lecture) Invest. Ophthal. Vis. Sci. 1974; 13: 713–724
  • Kinoshita J., Kador P., Datiles M. Aldose reductase in diabetic cataracts. JAMA 1981; 246: 257–261
  • Kinoshita J., Nishimuru C. The involvement of aldose reductase in diabetic complications. Diabetes/Metab. Rev. 1988; 4: 323–337
  • von Sallman L. The lens epithelium in the pathogenesis of cataract (The XIII Edward Jackson Memorial Lecture). Amer. J. Ophth. 1957; 44: 159–170
  • Grimes P., von Sallman L. Lens epithelium proliferation in sugar cataracts. Invest. Ophth. 1968; 7: 535–543
  • Marx J. Two major signal pathways linked. Nature 1993; 262: 988–990
  • Shi S., Unakar N. J., Wen Y., Tsui J., Behkor I. Transient elevation of aldose reductase mRNA in lens of rats developing galactose cataracts. Molec. Cell. Bioch. 1992; 115: 27–34
  • Darzynkiewicz Z. Differential staining of DNA and RNA in intact cells and isolated nuclei with acridine orange. Methods in Cell Biology, Vol. 33, Darzynkiewicz Crissman. Academic Press. 1990; 285–298
  • Crissman H. A., Darzynkiewicz Z., Tobey R. A., Steinkamp J. A. Correlated measurements of DNA, RNA, and protein in individual cells by flow cytometry. Science 1985; 228: 1321–1324
  • Gonzalez K., Udovichenko I. P., Cunnick J. M., Takemoto D. J. Protein kinase C in galactosemic and Tolrestat-treated lens epithelial cells. Curr. Eye Res. 1993; 12: 373–377
  • Brewitt B., Clark J. I. Growth and transparency in the lens, an epithelial tissue, stimulated by pulses of PDGF. Science 1988; 242: 777–779
  • Maluish A. E., Strong D. M. Lymphocyte proliferation. Manual of Clinical Laboratory immunology., N. R. Rose, H. Friedman, J. L. Fahey. American Society for Microbiology, Washington, DC 1986; 274–281
  • Ishizaki Y., Voyvodic J. T., Burne J. F., Raff M. C. Control of lens epithelial cell survival. J. Cell Biol. 1993; 121: 899–908
  • Jayat C., Ratinaud M. H. Cell cycle analysis by flow cytometry: Principles and applications. Biol. Cell. 1993; 78: 15–25
  • Darzynkiewicz Z., Bruno S., Del Bino G., Gorczyca W., Hotz M. A., Lassota P., Traganos F. Features of apoptotic cells measured by flow cytometry. Cytometry 1992; 13: 795–808
  • Bucala R., Model P., Cerami A. Modifications of DNA by reducing sugars: A possible mechanism for nucleic acid aging and age related dysfunction in gene expression. Proc. Natl. Acad. Sci. 1984; 81: 105–109
  • Pardee A. B. G1 events and regulation of cell proliferation. Science 1989; 246: 603–608
  • Afanasyer V. N., Korol B. A., Matylevich N. P., Pechatnikov V. A., Umansky S. R. The use of flow cytometry for the investigation of cell death. Cytometry 1993; 14: 603–609

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