A flow bio-reactor for studying the effects of haemodynamic forces on the morphology and rheology of cylindrically cultured endothelial cells

References

• Davies, P.F., 1995, Flow-mediated endothelial mechanotransduction. Physiology Reviews, 75, 519–560.
   PubMed Web of Science ®Google Scholar
• Chien, S., Li, S. and Shyy, Y.J., 1998, Effects of mechanical forces on signal transduction and gene expression in endothelial cells. Hypertension, 31, 162–169.
   PubMed Web of Science ®Google Scholar
• Helmlinger, G., Berk, B.C. and Nerem, R.M., 1996, Pulsatile and steady flow-induced calcium oscillations in single cultured endothelial cells. Journal of Vascular Research, 33, 360–369.
   Google Scholar
• Resnick, N., Einav, S., Chen-Konak, L., Zilberman, M., Yahav, H. and Shay-Salit, A., 2003, Hemodynamic forces as a stimulus for arteriogenesis. Endothelium, 10, 197–206.
   PubMedGoogle Scholar
• Hwang, J., Saha, A., Boo, Y.C., Sorescu, G.P., McNally, J.S., Holland, S.M., Dikalov, S., Giddens, D.P., Griendling, K.K., Harrison, D.G. and Jo, H., 2003, Oscillatory shear stress stimulates endothelial production of O2-from p47phox-dependent NAD(P)H oxidases, leading to monocyte adhesion. Journal of Biological Chemistry, 278, 47291–47298.
   PubMed Web of Science ®Google Scholar
• Sorescu, G.P., Sykes, M., Weiss, D., Platt, M.O., Saha, A., Hwang, J., Boyd, N., Boo, Y.C., Vega, J.D., Taylor, W.R. and Jo, H., 2003, Bone morphogenic protein 4 produced in endothelial cells by oscillatory shear stress stimulates an inflammatory response. Journal of Biological Chemistry, 278, 31128–31135.
   PubMed Web of Science ®Google Scholar
• White, C.R., Haidekker, M., Bao, X., Frangos, J.A., 2001, Temporal gradients in shear, but not spatial gradients, stimulate endothelial cell proliferation. Circulation, 103, 2508–2513.
   PubMed Web of Science ®Google Scholar
• Lewis, L.J., Hoak, J.C., Maca, R.D., Fry, G.L., 1973, Replication of human endothelial cells in culture. Science, 181, 453–454.
   PubMed Web of Science ®Google Scholar
• Jaffe, E.A., Nachman, R.L., Becker, C.G. and Minick, C.R., 1973, Culture of human endothelial cells derived from umbilical veins: Identification by morphologic criteria. Journal of Clinical Investigation, 52, 2745–2756.
   PubMed Web of Science ®Google Scholar
• Furchgott, R.F. and Zawadzki, J.V., 1980, The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature, 288, 373–376.
   PubMed Web of Science ®Google Scholar
• Alieva, I.B., Zemskov, E.A., Kireev, I.I., Gorshkov, B.A., Wiseman, D.A., Black, S.M. and Verin, A.D., 2010, Microtubules growth rate alteration in human endothelial cells. Journal of Biomedical Biotechnology, 671536. Doi:10.1155/2010/671536
   Google Scholar
• Helmke, B.P. and Davies, P.F., 2002, The cytoskeleton under external fluid mechanical forces: hemodynamic forces acting on the endothelium. Annals of Biomedical Engineering, 30, 284–286.
   Web of Science ®Google Scholar
• Chachisvilis, M., Zhang, Y.L. and Frangos, J.A., 2006, G-protein-coupled receptors sense fluid shear stress in endothelial cells. Proceedings of the National Academy of Sciences USA, 103, 15463–15468.
   PubMed Web of Science ®Google Scholar
• Patrick, C.W. Jr and Mclntire, L.V., 1995, Technique for visualization and quantification of three dimensional intracellular ion measurements in vascular endothelial cells. Review of Scientific Instruments, 66, 2476–2492.
   Google Scholar
• Birukova, A.A., Arce, F.T., Moldobaeva, N., Dudek, M., Garcia, J.G.N., Lal, R. and Birukov, K.G., 2009, Endothelial permeability is controlled by spatially defined cytoskeletal mechanics: AFM force mapping of pulmonary endothelial monolayer. Nanomedicine-UK, 5, 30–47.
   PubMed Web of Science ®Google Scholar
• Chouinard, J.A., Grenier, G., Khalil, A. and Vermette, P., 2008, Oxidized-LDL induce morphological changes and increase stiffness of endothelial cells. Experimental Cell Research, 314, 3007–3016.
   PubMed Web of Science ®Google Scholar
• Pesen, D. and Hoh, J.H., 2008, Micromechanical architecture of the endothelial cell cortex. Biophysical Journal, 88, 670–679.
   Google Scholar
• Kang, I., Panneerselvam, D., Panoskaltsis, V.P., Eppell, S.J., Marchant, R.E. and Doerschuk, C.M., 2008, Changes in the hyperelastic properties of endothelial cells induced by Tumor Necrosis Factor-α. Biophysical Journal, 94, 3273–3285.
   PubMed Web of Science ®Google Scholar
• Fabry, B., Maksym, G.N., Hubmayr, R.D., Butler, J.P. and Fredberg, J.J., 1999, Implications of heterogeneous bead behavior on cell mechanical properties measured with magnetic twisting cytometry. Journal of Magnetic Materials, 194, 120–125.
   Google Scholar
• Lele, T.P., Sero, J.E., Matthews, B.D., Kumar, S., Xia, S., Montoya-Zavala, M., Polte, T., Overby, D., Wang, N. and Ingber, D.E., 2007, Tools to study cell mechanics and mechanotransduction. Methods in Cell Biology, 83, 443–472.
   Google Scholar
• Khan, B.V., Parthasarathy, S.S., Alexander, R.W. and Medford, R.M., 1995, Modified low density lipoprotein and its constituents augment cytokine-activated vascular cell adhesion molecule-1 gene expression in human vascular endothelial cells. Journal of Clinical Investigations, 95, 1262–1270.
   PubMed Web of Science ®Google Scholar
• Sato, M., Suziki, K., Ueki, Y. and Ohashi, T., 2007, Microelastic mapping of living endothelial cells exposed to shear stress in relation to three-dimensional distribution of actin filaments. Acta Biomaterialia, 3, 331–339.
   Google Scholar
• Farcas, M.A., Rouleau, L., Fraser, R. and Leask, R.L., 2009, The development of 3-D, in vitro, endothelial culture models for the study of coronary artery disease. BioMedical Engineering OnLine, 8, 1–11.
   Google Scholar
• Barbee, K.A., Davies, P.F. and Lal, R., 1994, Shear stress-induced reorganization of the surface topography of living endothelial cells imaged by atomic force microscopy. Circulation Research, 74, 163–171.
   PubMed Web of Science ®Google Scholar
• Conway, D.E., Williams, M.R., Eskin, S.G. and McIntire, L.V., 2010, Endothelial cell responses to atheroprone flow are driven by two separate flow components, low time-average shear stress and fluid flow reversal. American Journal of Physiology – Heart and Circulatory Physiology, 298, H367–374.
   Google Scholar
• Blaha, M., Rencova, E., Blaha, V., Maly, R., Blazek, M., Studnicka, J., Andrys, C., Fatorova, I., Filip, S., Kasparova, M., Prochazkova, R., Maly, J., Zimova, R. and Langrova, H., 2009, The importance of rheological parameters in the therapy of microcirculatory disorders. Clinical Hemorheology and Microcirculation, 43, 37–46.
   Google Scholar
• Pullarkat, P.A., Fernandez, P.A., Ott, A., 2007, Rheological properties of the Eukaryotic cell cytoskeleton. Physics Reports, 449, 29–53.
   Web of Science ®Google Scholar
• Moriau, M., Lavenne-Pardonge, E., Crasborn, L., von Frenckell, R. and Col-Debeys, C., 1995, The treatment of severe or recurrent deep venous thrombosis. Beneficial effect of the co-administration of antiplatelet agents with or without rheological effects, and anticoagulants. Thrombosis Research, 78, 469–482.
   Google Scholar
• Lenormand, G., Millet, E., Fabry, B., Butler, J.P. and Fredberg, J.J., 2004, Linearity and time-scale invariance of the creep function in living cells. Journal of the Royal Society Interface, 1, 91–97.
   Google Scholar
• Fabry, B., Maksym, G.N., Shore, S.A., Moore, P.E., Panettieri, R.A. Jr, ButlerJ.P., and Fredberg, J.J., 2001, Signal transduction in smooth muscle: Selected contribution: Time course and heterogeneity of contractile responses in cultured human airway smooth muscle cells. Journal of Applied Physiology, 91, 986–994.
   PubMedGoogle Scholar
• Smith, P.G., Deng, L., Fredberg, J.J. and Maksym, G.N., 2003, Mechanical strain increases cell stiffness through cytoskeletal filament reorganization. American Journal of Physiology – Lung Cellular and Molecular Physiology, 285, 456–463.
   Google Scholar
• Trepat, X., Lenormand, G. and Fredberg, J.J., 2008, Universality in cell mechanics. Soft Matter, 4, 1750–1759.
   Google Scholar