Relative Quantitation of mRNA in β-Thalassemiamb E Using Real-Time Polymerase Chain Reaction

References

• Weatherall D. J., Clegg J. B. The Thalassemia Syndromes, 3^rd edition. Blackwell Scientific Publications, OxfordEngland 1981
   Google Scholar
• Thein S. L. Dominant β-thalassaemia: molecular basis and pathophysiology. Br. J. Haematol. 1992; 19: 213–236
   Google Scholar
• Kazazian H H., Jr., Boehm C. D. M molecular basis and prenatal diagnosis of β-thalassemia. Blood 1998; 72: 1107–1116
   Google Scholar
• Housman D., Forget B. G., Skoultchi A., Benz E. J., Jr. Quantitative deficiency of chain-specific globin mRNA in the thalassemia syndromes. Proc. Natl. Acad. Sci. USA 1973; 70: 1809–1813
   PubMed Web of Science ®Google Scholar
• Kacian D. L., Gambino R., Dow L. W., Grossbard E., Natta C., Ramirez F., Spiegelman S., Marks P. A., Bank A. Decreased globin messenger RNA in thalassemia detected by molecular hybridization. Proc. Natl. Acad. Sci. USA 1973; 70: 1886–1890
   PubMed Web of Science ®Google Scholar
• Orkin S. H., Kazazian H. H., Jr., Antonarakis S. E., Ostrer H., Goff S. C., Sexton J. P. Abnormal RNA processing due to the exon mutation of the βE-globin gene. Nature 1982; 300: 768–769
   PubMed Web of Science ®Google Scholar
• Fucharoen S., Winichagoon P., Pootrakul P., Piankijagum A., Wasi P. Variable severity of Southeast Asian β-thalassemia/Hb E disease. Birth Defects 1988; 23: 241–248, 5A
   Google Scholar
• Kazazian H. H., Jr., Orkin S. H., Antonarakis S. E., Sexton J. P., Boehm C. D., Goff S. C., Waber P. G. Molecular characterization of seven β-thalassemia mutations in Asian Indians. EMBO J 1984; 3: 593–596
   PubMed Web of Science ®Google Scholar
• Capellini M. D., Fiorelli G., Bernini L. F. Interaction between homozygous β-thala-saemia and the Swiss type of hereditary persistence of fetal haemoglobin. Br. J. Haematol. 1981; 48: 561–572
   PubMedGoogle Scholar
• Winichagoon P., Fucharoen S., Weatherall D. J., Wasi P. Concomitant inheritance of α-thalassemia in β-thalassemia/Hb E. Am. J. Haematol. 1985; 20: 217–222
   PubMed Web of Science ®Google Scholar
• Winichagoon P., Thonglairoam V., Fucharoen S., Wilairat P., Fukumaki Y., Wasi P. Severity in β-thalassaemia/Hb E syndrome: implication of genetic factors. Br. J. Haematol. 1993; 83: 633–639
   PubMed Web of Science ®Google Scholar
• Traeger J., Wood W. G., Clegg J. B., Weatherall D. J., Wasi P. Defective synthesis of Hb E is due to reduced levels of βE mRNA. Nature (London) 1980; 288: 497–499
   PubMed Web of Science ®Google Scholar
• Benz E. J., Berman B. W., Tokonow B. L., Coupal E., Coates T., Boxer L. A., Altman A., Adams J. G. Molecular analysis of the β-thalassemia phenotype associated with the inheritance of Hb E (α2β226Glulys). J. Clin. Invest. 1981; 68: 118–126
   PubMed Web of Science ®Google Scholar
• Culbertson M. R. RNA surveillance; unforseen consequences for genye expression, inherited genetic disorders and cancer. Trends Genet 1999; 15: 74–80
   PubMed Web of Science ®Google Scholar
• Winichagoon P., Saechan V., Sripanich R., Nopparatana C., Kanokpongsakdi S., Maggio A., Fucharoen S. Prenatal diagnosis of β-thalassemia by reverse dot-blot hybridization. Prenat. Diagn. 1999; 19: 428–435
   PubMed Web of Science ®Google Scholar
• Chomczynski P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques 1993; 15: 532–537
   PubMed Web of Science ®Google Scholar
• Heid C. A., Stevens J., Livak K. J., Williams P. M. Real-time quantitative PCR. Genome Res 1996; 6: 986–994
   PubMed Web of Science ®Google Scholar
• Livak K. J., Flood S. J., Marmaro J., Giusti W., Deetz K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Meth. Appli. 1995; 4: 357–362
   PubMedGoogle Scholar
• Gibson U. E. M., Held C. A., Williams P. M. A novel method for real-time quantitative RT-PCR. Genome Res 1996; 6: 995–1001
   PubMed Web of Science ®Google Scholar
• Heneine W., Yamamoto S., Switzer W. M., Spira T. J., Folks R. M. Detection of reverse transcriptase by a highly sensitive assay in sera from persons infected with human immunodeficiency virus type 1. J. Infect. Dis. 1995; 171: 1210–1216
   PubMedGoogle Scholar
• Traeger J., Winichagoon P., Wood W. G. Instability of βE messenger RNA during erythroid cell maturation in Hemoglobin E homozygotes. J. Clin. Invest. 1982; 69: 1050–1053
   PubMed Web of Science ®Google Scholar
• Hunt D. M., Higgs D. R., Winichagoon P., Clegg J. B., Weatherall D. J. Haemoglobin Constant Spring has an unstable a chain messenger RNA. Br. J. Haematol. 1982; 51: 405–413
   PubMed Web of Science ®Google Scholar
• Winichagoon P., Fucharoen S., Wilairat P., Chihara K., Fukumaki Y. Role of alternatively spliced βE-globin mRNA on clinical severity of β-thalassemia/Hemoglobin E disease. Southeast Asian J. Trop. Med. Public Health 1995; 26: 241–245
   PubMedGoogle Scholar
• Smetanina N. S., Gu L H., Schilirò G., Di Cataldo A., Testa R., Jakovlevska Z., Efremov G. D., Huisman T. H.J. Relative levels of α-, β-, and γ-mRNA from patients with severe and intermediate β-thalassemia major. Acta Haematol 1997; 97: 205–210
   PubMed Web of Science ®Google Scholar
• Smetanina N. S., Molchanova T. P., Huisman T. H.J. Analysis of mRNA from red cells of patients with and hemoglobinopathies. Hemoglobin 1997; 21: 437–467
   PubMed Web of Science ®Google Scholar