5
Views
10
CrossRef citations to date
0
Altmetric
Original Article

Transneuronal Transport of Wheatgerm Agglutinin Conjugated with Horseradish Peroxidase in the Somatosensory System of the Rat: A Light- and Electron-Microscopic Study

, , &
Pages 119-137 | Published online: 10 Jul 2009

References

  • Barondes S. H. Lectins: Their multiple endogenous cellular functions. Ann. Rev. Biochem. 1981; 50: 207–231
  • Brushart T. M., Mesulam M. ‐M. Transganglionic demonstration of central sensory projections from skin and muscle with HRP‐lectin conjugates. Neurosci. Lett. 1982; 17: 1–6
  • Carson K. A., Lucas W. J., Gregg J. M., Hanker J. S. Facilitated ultracytochemical demonstration, of retrograde axonal transport of horseradish peroxidase in peripheral nerve. Histochemistry 1980; 67: 113–124
  • Carson K. A., Mesulam M. ‐M. Ultrastructural evidence in mice that transganglionically transported horseradish peroxidase‐wheat germ agglutinin conjugate reaches the intraspinal terminations of sensory neurons. Neurosci. Lett. 1982a; 29: 201–206
  • Carson K. A., Mesulam M. ‐M. Electron microscopic demonstration of neural connections using horseradish peroxidase: A comparison of the tetramethylbenzidine procedure with seven other histochemical methods. J. Histochem. Cytochem. 1982b; 30: 425–435
  • Ellis L. C., Diorio J. P., Rustioni A. Thalamic projecting neurons in the feline nucleus cuneatus: A combined horseradish peroxidase and high voltage electron microscopic study. J. Neurocytol. 1982; 11: 3–17
  • Goldstein I. J., Hayes C. E. Lectins: Carbohydrate‐binding proteins of plants and animals. Adv. Carbohyd:. Chem. Biochem. 1978; 35: 127–340
  • Gonatas N. K., Harper C., Mizutani T., Gonatas J. O. Superior sensitivity of conjugates of horseradish peroxidase with wheat germ agglutinin for studies of retrograde axonal transport. J. Histochem. Cytochem. 1979; 27: 728–734
  • Gerfen C. R., O'Leary D. D.M., Cowan W. M. A note on the transneuronal transport of wheat germ agglutinin‐conjugated horseradish peroxidase in the avian and rodent visual systems. Exp. Brain Res. 1982; 48: 443–448
  • Hanker J. S., Yates P. E., Metz C. B., Rustioni A. A new specific, sensitive and non‐carcinogenic reagent for the demonstration of horseradish peroxidase. Histochem. J. 1977; 9: 789–792
  • Harrison P. J., Hultborn H., Jankowska E., Katz R., Storai B., Zytnicki D. Labeling of interneurons by retrograde transsynaptic transport of horseradish peroxidase from motorneurons in rats and cats. Neurosci. Lett. 1984; 45: 15–19
  • Itaya S. K., van Hoesen G. W. WGA‐HRP as a transneuronal marker in the visual pathways of monkey and rat. Brain Res. 1982; 236: 199–204
  • Itoh K., Yasui Y., Takada M., Mitani A., Kaneko T., Sugimoto T., Mizuno N. An antergorade‐retrograde transneuronal transport of conjugates of wheat germ agglutinin with horseradish peroxidase (WGA‐HRP): Labeling of neurons in the reticular nucleus of the thalamus with WGA‐HRP injected into the posterior column nuclei in the cat. Brain Res. 1984; 323: 185–187
  • Jones E. G. Some aspects of the organization of the thalamic reticular complex. J. Comp. Neurol. 1975; 162: 285–308
  • Kristensson K., Olsson Y. Retrograde axonal transport of a protein. Brain Res. 1971; 29: 43–47
  • Lasek R. J., Joseph B. S., Whitlock D. G. Evaluation of a radioautographic neuroanatomical tracing method. Brain Res. 1968; 8: 319–336
  • La Vail J. H., La Vail M. M. The retrograde intraaxonal transport of horseradish peroxidase in the chick visual system: A light and electron microscopic study. J. Comp. Neurol. 1972; 157: 303–358
  • Mesulam M. ‐M. Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: A non‐carcinogenic blue reaction‐product with superior sensitivity for visualizing neural afferents and efferents. J. Histochem. Cytochem. 1978; 26: 106–117
  • Mesulam M. ‐M., Rosene D. L. Sensitivity in horseradish peroxidase neurohistochemistry: A comparative and quantitative analysis of nine methods. J. Histochem. Cytochem. 1979; 27: 763–773
  • Peschanski M., Ralston J. J., III. Light and electron microscopic evidence of transneuronal labeling with WGA‐HRP to trace somatosensory pathways to the thalamus. J. Comp. Neurol. 1985; 236: 29–41
  • Pugh W. W., Kalia M. Differential uptake of peroxidase (HRP) and peroxidase‐lectin (HRP‐WGA) conjugate injected in the nodose ganglion of the cat. J. Histochem. Cytochem. 1982; 30: 887–894
  • Romanovicz D. K., Hanker J. S. Wafer embedding: Specimen selection in electron microscopic cytochemistry with osmiophilic polymers. Histochem. J. 1977; 9: 317–327
  • Sakumoto T., Nagai T., Kimura H., Maeda T. Electron microscopic visualization of tetramethyl benzidine reaction product on horseradish peroxidase neurohistochemistry. Cell. Mol. Biol. 1980; 26: 211–216
  • Schnyder H., Kunzle H. Differential labeling in neuronal tracing with wheat germ agglutinin. Neurosci. Lett. 1983; 35: 115–120
  • Schwab M. E., Javoy‐Agid F., Agid Y. Labeled wheat germ agglutinin (WGA) as a new, highly sensitive retrograde tracer in the rat brain hippocampal system. Brain Res. 1978; 152: 145–150
  • Spreafico R., Cheema S., Ellis L. C., Jr., Rustioni A. On comparison of horseradish peroxidase visualization methods. J. Histochem. Cytochem. 1982; 5: 487–488
  • Spreafico R., Frassoni C. Projections from the nucleus reticularis thalami to specific and non‐specific thalamic nuclei of the rat: An HRP study. Neurosci. Lett. (Suppl.) 1983; 14: S354
  • Trojanowski J. Q. Native and derivatized lectins for in vivo studies of neuronal connectivity and neuronal cell biology. J. Neurosci. Meth. 1983; 9: 185–204
  • Welker C. Microelectrode delineation of fine grain somatotopic organization of SmI cerebral neocortex in albino rat. Brain Res. 1971; 26: 259–275

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.