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Review Article

β Galactosidases and their potential applications: a review

Pages 41-62 | Accepted 20 Aug 2009, Published online: 09 Feb 2010

References

  • Akgol S, Kacar Y, Denizli A, Aria MY. 2001. Hydrolysis of sucrose by invertase immobilized onto novel magnetic polyvinyl alcohol microspheres. Food Chem 74: 281–288.
  • Akhtar S, Khan AA, Husain Q. 2005. Simultaneous purification and immobilization of bitter gourd (Momordica charantia) peroxidases on bioaffinity support. J Chem Technol Biotechnol 80: 198–205.
  • Albayrak N, Yang ST. 2002. Immobilization of Aspergillus oryzae β galactosidase on tosylated cotton cloth. Enzy Microb Technol 31: 371–383.
  • Alcantara PHN, Martim L, Silva CO, Dietrich SMC, Buckeridge MS. 2006. Purification of a β galactosidase from cotyledons of Hymenaea courbaril L. (Leguminosae): enzyme properties and biological function. Plant Physiol Biochem 44: 619–627.
  • Al-Muftah AE, Abu-Reesh IM. 2005. Effects of internal mass transfer and product inhibition on a simulated immobilized enzyme-catalyzed reactor for lactose hydrolysis. Biochem Eng J 23: 139–153.
  • Amaral IPG, Carnciro-da-Cunha MG, Carvalho, Jr, LB Benzerra, RS. 2006. Fish trypsin immobilized on ferromagnetic Dacron. Proc Biochem 41: 1213–1216.
  • Amarita F, Fernandez CR, Alkorta F. 1997. Hybrid biosensors to estimate lactose in milk. Analyt Chim Acta 349: 153–158.
  • Anderson D, Nguyen T, Lai PK, Amiji M. 2001. Evaluation of the permeability and blood-compatibility of membranes formed by physical interpenetration of chitosan with PEO/PPO/PEO triblock copolymers. J Appl Polym Sci 80: 1274–1284.
  • Arribas JC, Herrero AG, Martín-Lomas M, Cañada FJ, He S, Withers SG. 2000. Differential mechanism-based labeling and unequivocal activity assignment of the two active sites of intestinal lactase/phlorizin hydrolase. Eur J Biochem 267: 6996–7005.
  • Arunachalam KD. 2004. Role of bifidobacteria in nutrition, medicine and technology. Nutr Res 19: 1559–1597.
  • Audic JL, Chaufer B, Daufin G. 2003. Non-food applications of milk components and dairy co-products: a review. Lait 83: 417–438.
  • Ball JC, Puckett LG, Bachas LG. 2003. Covalent immobilization of beta-galactosidase onto a gold-coated magnetoelastic transducer via a self-assembled monolayer: toward a magnetoelastic biosensor. Anal Chem 75: 6932–6937.
  • Bayramoglu G, Yilmaz M, Arica MY. 2004. Immobilization of a thermostable α-amylase onto reactive membranes: kinetics characterization and application to continuous starch hydrolysis. Food Chem 84: 591–599.
  • Bayramoglu G, Tunali Y, Arica MY. 2007. Immobilization of β galactosidase onto magnetic poly (GMA-MMA) beads for hydrolysis of lactose in bed reactor. Cataly Comm 8: 1094–1101.
  • Betageri GV, Kulkarni SB. 1999. Preparation of liposomes. In: Arsjadu R, ed. Microsphere, Microcapsules and Liposomes. London: Citus Books 4: 489–521.
  • Betancor L, López-Gallego F, Hidalgo A, Fuentes M, Podrasky O, Kuncova G, Guisán JM, Fernández-Lafuente R. 2005. Advantages of the pre-immobilization of enzymes on porous supports for their entrapment in sol-gels. Biomacromolecules 6: 1027–1030.
  • Betancor L, Luckarift HR, Seo JH, Brand O, Spain JC. 2008. Three-dimensional immobilization of beta-galactosidase on a silicon surface. Biotechnol Bioeng 99: 261–267.
  • Biró E, Németh AS, Sisak C, Feczkó T, Gyenis J. 2008. Preparation of chitosan particles suitable for enzyme immobilization. J Biochem Biophys Methods 70: 1240–1246.
  • Biswas S, Kayastha AM, Seckler R. 2003. Purification and characterization of a thermostable beta-galactosidase from kidney beans (Phaseolus vulgaris L.) cv. PDR14. J Plant Physiol 160: 327–337.
  • Blandino A, Macías M, Cantero D. 2000. Glucose oxidase release from calcium alginate gel capsules. Enzyme Microb Technol 27: 319–324.
  • Bodalo A, Gomez JL, Gomez E, Bastida J, Maximo MF. 1995. Fluidized bed reactors operating with immobilized enzyme systems: Design model and its experimental verification. Enzy Microb Technol 17: 915–922.
  • Bódalo A, Gómez JL, Gómez E, Bastida J, Máximo MF, Montiel MC. 2001. Ultrafiltration membrane reactors for enzymatic resolution of amino acids: design model and optimization. Enzyme Microb Technol 28: 355–361.
  • Boon MA, Janssen AE, van’t Riet K. 2000. Effect of temperature and enzyme origin on the enzymatic synthesis of oligosaccharides. Enzyme Microb Technol 26: 271–281.
  • Buckeridge MS, Reid JS. 1994. Purification and properties of a novel beta-galactosidase or exo-(1→4)-beta-D-galactanase from the cotyledons of germinated Lupinus angustifolius L. seeds. Planta 192: 502–511.
  • Bulhões AC, Goldani HA, Oliveira FS, Matte US, Mazzuca RB, Silveira TR. 2007. Correlation between lactose absorption and the C/T-13910 and G/A-22018 mutations of the lactase-phlorizin hydrolase (LCT) gene in adult-type hypolactasia. Braz J Med Biol Res 40: 1441–1446.
  • Burin L, Buera MP. 2002. β Galactosidase activity as affected by apparent pH and physical properties of reduced moisture systems. Enzy Microb Technol 30: 367–373.
  • Calabro V, Curcio S, Iorio G. 2002. A theoretical analysis of transport phenomena in a hollow-fibre membrane bioreactor with immobilized biocatalyst. J Memb Sci 206: 217–241.
  • Calonder C, Tie Y, Van Tassel PR. 2001. History dependence of protein adsorption kinetics. Proc Natl Acad Sci USA 98: 10664–10669.
  • Cammarota MC, Freire DM. 2006. A review on hydrolytic enzymes in the treatment of wastewater with high oil and grease content. Bioresour Technol 97: 2195–2210.
  • Cappello G, Marzio L. 2005. Rifaximin in patients with lactose intolerance. Dig Liver Dis 37: 316–319.
  • Caprio C, Gonzalez P, Ruales J, Batista-Viera F. 2000. Bone bound enzymes for food industry applications. Food Chem 68: 525–529.
  • Carrara CR, Mammarella EJ, Rubiolo AC. 2003. Prediction of the fixed bed reactor behavior using dispersion and plug-flow models with different kinetic for immobilized enzyme. Chem Eng J 92: 123–129.
  • Cataldi TRI, Angelotti M, Bianco G. 2003. Determination of mono and disaccharides in milk and milk products by high performance anion-exchange chromatography with pulsed amperometric detection. Analyt Chim Acta 485: 43–49.
  • Chen CW, Ou-Yang CC. 2004. Bounded water kinetic model of beta-galactosidase in reverse micelles. Bioprocess Biosyst Eng 26: 307–313.
  • Chen W, Chen H, Xia Y, Zhao J, Tian F, Zhang H. 2008. Production, purification, and characterization of a potential thermostable galactosidase for milk lactose hydrolysis from Bacillus stearothermophilus. J Dairy Sci 91: 1751–1758.
  • Chen W, Chen H, Xia Y, Yang J, Zhao J, Tian F, Zhang HP, Zhang H. 2009. Immobilization of recombinant thermostable beta-galactosidase from Bacillus stearothermophilus for lactose hydrolysis in milk. J Dairy Sci 92: 491–498.
  • Cieslinski H, Kur J, Bialkowska A, Baran I, Makowski K, Turkiewicz M. 2005. Cloning, expression, and purification of a recombinant cold-adapted beta-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b. Protein Expr Purif 39: 27–34.
  • Cooper MA. 2002. Optical biosensors in drug discovery. Nat Rev Drug Discov 1: 515–528.
  • Coradin T, Livage J. 2003. Mesoporous alginate/silica biocomposites for enzyme immobilization. C R Chim 6: 147–152.
  • Corazza GR, Ginaldi L, Furia N, Marani-Toro G, Di Giammartino D, Quaglino D. 1997. The impact of HIV infection on lactose absorptive capacity. J Infect 35: 31–35.
  • Cunha AG, Fernández-Lorente G, Bevilaqua JV, Destain J, Paiva LM, Freire DM, Fernández-Lafuente R, Guisán JM. 2008. Immobilization of Yarrowia lipolytica lipase—a comparison of stability of physical adsorption and covalent attachment techniques. Appl Biochem Biotechnol 146: 49–56.
  • Curcio S, Calabro V, Iorio G. 2006. A theoretical and experimental analysis of a membrane bioreactor performance in recycle configuration. J Memb Sci 273: 129–142.
  • Dalal S, Gupta MN. 2007. Treatment of phenolic wastewater by horseradish peroxidase immobilized by bioaffinity layering. Chemosphere 67: 741–747.
  • De Veau EJI, Gross KC, Huber DJ, Watada AE. 1993. Degradation and solubilization of pectin by β galactosidases purified from avocado mesocarp. Physiol Plantar 87: 279–285.
  • Delgado AL, Hidalgo-Lara ME, Montes-Horcasitas MC. 2006. Hydrolysis of sucrose by invertase immobilized on nylon-6 microbeads. Food Chem 99: 299–304.
  • Deng HT, Xu ZK, Liu ZM, Wu J, Ye P. 2004. Adsorption immobilization of Candida rugosa lipases on polypropylene hollow fibre microfilters. Enzy Microb Technol 35: 437–443.
  • Desai PD, Dave AM, Devi S. 2004. Entrapment of lipase into k-carrageenan beads and its use in hydrolysis of olive oil in biphasic system. J Molec Catal B: Enzym 31: 143–150.
  • Di Lauro B, Strazzulli A, Perugino G, La Cara F, Bedini E, Corsaro MM, Rossi M, Moracci M. 2008. Isolation and characterization of a new family 42 beta-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: identification of the active site residues. Biochim Biophys Acta 1784: 292–301.
  • Diano N, Grano V, Rossi S, Bencivenga U, Portaccio M, Amato U, Carfora F, Lepore M, Gaeta FS, Mita DG. 2004. Hollow-fiber enzyme reactor operating under nonisothermal conditions. Biotechnol Prog 20: 457–466.
  • Dominques L, Lima N, Teixeira J.A. 2005. Aspergillus niger β galactosidase production by yeast in a continuous high cell density reactor. Process Biochem 40: 1151–1154.
  • Duran N, Rosa MA, Annibale AD, Gianfreda L. 2002. Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review. Enzyme Microb Technol 31: 907–931.
  • Dwevedi A, Kayastha AM. 2009a. A beta-galactosidase from pea seeds (PsBGAL): purification, stabilization, catalytic energetics, conformational heterogeneity, and its significance. J Agric Food Chem 57: 7086–7096.
  • Dwevedi A, Kayastha AM. 2009b. Stabilization of beta-galactosidase (from peas) by immobilization onto amberlite MB-150 beads and its application in lactose hydrolysis. J Agric Food Chem 57: 682–688.
  • Edwards M, Bowman YJ, Dea IC, Reid JS. 1988. A beta-D-galactosidase from nasturtium (Tropaeolum majus L.) cotyledons. Purification, properties, and demonstration that xyloglucan is the natural substrate. J Biol Chem 263: 4333–4337.
  • El-Masry MM, Maio AD, Martelli PL, Casadio R, Moustafa AB, Rossi S, Mita DG. 2001a. Influence of the immobilization process on the activity of β galactosidase bound to nylon membranes grafted with glycidyl methacrylate. Part I. Isothermal behavior. J Molec Catal B: Enzym 16: 175–189.
  • El-Masry MM, De Maio A, Portaccio M, Di Martino S, Bencivenga U, Rossi S, Gaeta FS, Mita DG. 2001b. Isothermal and non-isothermal characterization of catalytic nylon membranes chemically grafted: dependence on the grafting percentage. Enzyme Microb Technol 28: 773–784.
  • Elnashar MM, Yassin MA. 2009. Lactose Hydrolysis by beta-Galactosidase Covalently Immobilized to Thermally Stable Biopolymers. Appl Biochem Biotechnol 159: 426–437.
  • Flickinger MC, Drew SW. 1999. In: Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation. John Wiley & Sons, New York. Biocataly Biosepar 1–5: 1545–1554.
  • Flood MT, Kondo M. 2004. Toxicity evaluation of a beta-galactosidase preparation produced by Penicillium multicolor. Regul Toxicol Pharmacol 40: 281–292.
  • Fraguas LF, Bolon V, Viera FB. 1999. Affinity immobilization of β galactosidase from Aspergillus oryzae on lectin-based adsorbents. In: Lectins, Biology, Biochemistry, Clinical Biochemistry. (Driessene E, Beeckmans S, Thorkild C, Hansen B, eds). TEXTOP, Hellerup, Denmark. 13.
  • Furlan SA, Schneider ALS, Merkle R, Carvalho-Jonas MD, Jonas R. 2000. Formulation of a lactose-free, low cost culture medium for the production of β-D-galactosidase by Kluyveromyces marxianus. Biotechnol Lett 22: 589–593.
  • Ganesh K, Joshi JB, Sawant SB. 2000. Cellulase deactivation in a stirred reactor. Biocheml Eng J 4: 137–141.
  • Gåserød O, Sannes A, Skjåk-Braek G. 1999. Microcapsules of alginate-chitosan. II. A study of capsule stability and permeability. Biomaterials 20: 773–783.
  • Genari AN, Passos FV, Passos FM. 2003. Configuration of a bioreactor for milk lactose hydrolysis. J Dairy Sci 86: 2783–2789.
  • Giorno L, Drioli E. 2000. Biocatalytic membrane reactors: applications and perspectives. Trends Biotechnol 18: 339–349.
  • Girelli AM, Mattei E. 2005. Application of immobilized enzyme reactor in on-line high performance liquid chromatography: a review. J Chromatogr B Analyt Technol Biomed Life Sci 819: 3–16.
  • Goddard JM, Talbert JN, Hotchkiss JH. 2007. Covalent attachment of lactase to low-density polyethylene films. J Food Sci 72: E036–E041.
  • Goktug T, Sezginturk MK, Dinckaya E. 2005. Glucose oxidase-β galactosidase hybrid biosensor based on glassy carbon electrode modified with mercury for lactose determination. Analyt Chim Acta 551: 51–56.
  • Grosová Z, Rosenberg M, Rebros M, Sipocz M, Sedlácková B. 2008. Entrapment of beta-galactosidase in polyvinylalcohol hydrogel. Biotechnol Lett 30: 763–767.
  • Grosova Z, Rosenberg M, Rebros M. 2008a. Perspectives and applications of immobilized β galactosidase in food industry: a review. Czech J Food Sci 26: 1–14.
  • Grosova Z, Rosenberg M, Rebros M, Sipocz M, Sedlackova B. 2008b. Entrapment of β galactosidase in polyvinyl alcohol hydrogel. Biotechnol Lett 30: 763–767.
  • Guadix A, Camacho F, Guadix EM. 2006. Production of whey protein hydrolysates with reduced antigenicity in a stable membrane reactor. J Food Eng 72: 398–405.
  • Hadfield KA, Dang T, Guis M, Pech JC, Bouzayen M, Bennett AB. 2000. Characterization of ripening-regulated cDNAs and their expression in ethylene-suppressed charentais melon fruit. Plant Physiol 122: 977–983.
  • Haider T, Husain Q. 2007a. Preparation of lactose free milk by using ammonium sulphate fractionated proteins from almonds. J Sci Food Agric 87: 1278–1283.
  • Haider T, Husain Q. 2007b. Calcium alginate entrapped preparations of Aspergillus oryzae beta galactosidase: its stability and applications in the hydrolysis of lactose. Int J Biol Macromol 41: 72–80.
  • Haider T, Husain Q. 2008. Concanavalin A layered calcium alginate-starch beads immobilized beta galactosidase as a therapeutic agent for lactose intolerant patients. Int J Pharm 359: 1–6.
  • Haider T, Husain Q. 2009a. Hydrolysis of milk/whey lactose by β galactosidase: A comparative study of stirred batch process and packed bed reactor prepared with calcium alginate entrapped enzyme. Chem Eng Proc: Process Intensification 48: 575–580.
  • Haider T, Husain Q. 2009b. β Galactosidase by bioaffinity adsorption on immobilization of concanavalin A layered calcium alginate-starch hybrid beads for the hydrolysis of lactose from whey/milk. Int Dairy J 19: 172–177.
  • Haider T, Husain Q. 2009c. Immobilization of β galactosidase from Aspergillus oryzae via immunoaffinity support. Biochem Eng J 43: 307–314.
  • Hartmann M. 2005. Ordered mesoporous materials for bioadsorption and biocatalysis. Chem Mater 17: 4577–4593.
  • Hatzinikolaou DG, Katsifas E, Mamma D, Karagouni AD, Christakopoulos P, Kekos D. 2005. Modeling of the simultaneous hydrolysis-ultrafiltration of whey permeate by a thermostable β galactosidase from Aspergillus niger. Biochem Eng J 24: 161–172.
  • Heise C, Dandekar S, Kumar P, Duplantier R, Donovan RM, Halsted CH. 1991. Human immunodeficiency virus infection of enterocytes and mononuclear cells in human jejunal mucosa. Gastroenterology 100: 1521–1527.
  • Hennink WE, van Nostrum CF. 2002. Novel crosslinking methods to design hydrogels. Adv Drug Deliv Rev 54: 13–36.
  • Hernaiz MJ, Crout DH. 2000. Immobilization/stabilization on Eupergit C of the beta-galactosidase from B. circulans and an alpha-galactosidase from Aspergillus oryzae. Enzyme Microb Technol 27: 26–32.
  • Heyman MB; Committee on Nutrition. 2006. Lactose intolerance in infants, children, and adolescents. Pediatrics 118: 1279–1286.
  • Hsu CA, Yu RC, Chou CC. 2005. Production of beta-galactosidase by Bifidobacteria as influenced by various culture conditions. Int J Food Microbiol 104: 197–206.
  • Huang NP, Voros J, Paul SM, Textor M, Spencer ND. 2002. Biotin-derivatized poly (L-lysine)-g-poly (ethylene glycol): A novel polymeric interface for bioaffinity sensing. Langmuir 18: 220–230.
  • Ishchuk OP, Dmytruk KV, Rohulya OV, Voronovsky AY, Abbas CA, Sibirny AA. 2007. Development of a promoter assay system for the flavinogenic yeast Candida famata based on the Kluyveromyces lactis β galactosidase LAC4 reporter gene. Enzy Microb Technol 42: 208–215.
  • Jacob R, Peters K, Naim HY. 2002. The prosequence of human lactase-phlorizin hydrolase modulates the folding of the mature enzyme. J Biol Chem 277: 8217–8225.
  • Jacob R, Weiner JR, Stadge S, Naim HY. 2000. Additional N-glycosylation and its impact on the folding of intestinal lactase-phlorizin hydrolase. J Biol Chem 275: 10630–10637.
  • Jain A, Gupta Y, Jain SK. 2007. Perspectives of biodegradable natural polysaccharides for site-specific drug delivery to the colon. J Pharm Pharm Sci 10: 86–128.
  • Jenkins DM, Delwiche MJ. 2003. Adaptation of a manometric biosensor to measure glucose and lactose. Biosens Bioelectron 18: 101–107.
  • Joshi MS, Gowda LR, Katwa LC, Bhatt SG. 1987. Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by cetyl trimethyl ammonium bromide. Biotechnol Lett 9: 549–554.
  • Juang RS, FC Tseng, RL. 2001. Solute adsorption and enzyme immobilization on chitosan beads prepared from shrimp shell wastes. Bioresour Technol 80: 187–193.
  • Jurado E, Camacho F, Luzon G, Vicaria JM. 2004. Kinetic models of activity for β galactosidases: Influence of pH, ionic concentration and temperature. Enzy Microb Technol 34: 33–40.
  • Jurado E, Camacho F, Luzon G, Vicaria JM. 2006. Influence of the hollow-fibre membrane on the stability of β galactosidase and on lactose hydrolysis: Kinetic models including adsorption of the enzyme onto the membrane. Enzy Microb Technol 39: 1008–1015.
  • Jurado E, Camacho F, Luzon G, Vicaria JM. 2002. A new kinetic model proposed for enzymatic hydrolysis of lactose by a β galactosidase from Kluyveromyces fragilis. Enzy Microb Technol 31: 300–309.
  • Jurado E, Camacho F, Luzón G, Vicaria JM. 2005. Kinetic and enzymatic adsorption model in a recirculation hollow-fibre bioreactor. Bioprocess Biosyst Eng 28: 27–36.
  • Kallenberg AI, Rantwijk FV, Sheldon RA. 2005. Immobilization of penicillin G acylase: The key to optimum performance. Adv Syn Catal 347: 905–926.
  • Kang IK, Suh SG, Gross KC, Byun JK. 1994. N-terminal amino acid sequence of persimmon fruit beta-galactosidase. Plant Physiol 105: 975–979.
  • Karasova-Lipovova P, Strnad H, Spiwok V, Mala S, Kralova B, Russell NJ. 2003. The cloning, purification and characterisation of a cold-active β galactosidase from the psychrotolerant Antarctic bacterium Arthrobacter sp. C2-2. Enzy Microb Technol 33: 836–844.
  • Kenausis GL, Voros J, Elbert DL, Huang N, Hofer R, Taylor LR, Textor M, Hubbell JA, Spencer ND. 2000. Poly (L-lysine)-g-poly (ethylene glycol) layers on metal oxide surfaces: Attachment mechanism and effects of polymer architecture on resistance to protein adsorption. J Phys Chem B 104: 3298–3309.
  • Kerber M, Oberkanins C, Kriegshäuser G, Kollerits B, Dossenbach-Glaninger A, Fuchs D, Ledochowski M. 2007. Hydrogen breath testing versus LCT genotyping for the diagnosis of lactose intolerance: a matter of age? Clin Chim Acta 383: 91–96.
  • Khan AA, Akhtar S, Husain Q. 2005. Simultaneous purification and immobilization of mushroom tyrosinase on immunoaffinity support. Proc Biochem 40: 2379–2386.
  • Khan AA, Akhtar S, Husain Q. 2006. Direct immobilization of polyphenol oxidases on Celite 545 from ammonium sulphate fractionated proteins of potato (Solanum tuberosum). J Molec Catal B: Enzym 40: 58–63.
  • Kierstan M, Bucke C. 2000. The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels. Reprinted from Biotechnology and Bioengineering, Vol. XIX, No. 3, Pages 387-397 (1977). Biotechnol Bioeng 67: 726–736.
  • Kim CS, Ji ES, Oh DK. 2004. A new kinetic model of recombinant beta-galactosidase from Kluyveromyces lactis for both hydrolysis and transgalactosylation reactions. Biochem Biophys Res Commun 316: 738–743.
  • Kim CK, Chung HS, Lee MK, Choi LN, Kim MH. 1999. Development of dried liposomes containing beta-galactosidase for the digestion of lactose in milk. Int J Pharm 183: 185–193.
  • Kim JW, Rajagopal SN. 2000. Isolation and characterization of beta-galactosidase from Lactobacillus crispatus. Folia Microbiol (Praha) 45: 29–34.
  • Kitagawa Y, Kanayama Y, Yamaki S. 1995. Isolation of β galactosidase fraction from Japanese pear: Activity against cell wall polysaccharides. Physiol Plantar 93: 545–550.
  • Klewicki R. 2007. Formation of gal-sorbitol during lactose hydrolysis with β galactosidase. Food Chem 100: 1196–1201.
  • Knezevic Z, Kukic G, Vukovic M, Bugarski B, Obradovic B. 2004. Operating regime of a biphasic oil/aqueous hollow-fibre reactor with immobilized lipase for oil hydrolysis. Food Chem 39: 1377–1385.
  • Konno H, Katoh K. 1992. An extracellular β galactosidase secreted from cell suspension cultures of carrot: Its purification and involvement in cell wall polysaccharide hydrolysis. Physiol Plantar 85: 507–514.
  • Konno H, Tsumuki H. 1993. Purification of β galactosidase from rice shoots and its involvement in hydrolysis of the natural substrate in cell walls. Physiol Plantar 89: 40–47.
  • Krajewska B. 2004. Application of chitin and chitosan-based materials for enzyme immobilizations: A review. Enzy Microb Technol 35: 126–139.
  • Kulshrestha Y, Husain Q. 2006a. Bioaffinity-based an inexpensive and high yield procedure for the immobilization of turnip (Brassica rapa) peroxidase. Biomol Eng 23: 291–297.
  • Kulshrestha Y, Husain Q. 2006b. Adsorption of peroxidases on DEAE-cellulose directly from ammonium sulphate fractionated proteins of bitter gourd. Enzy Microb Technol 38: 470–477.
  • Kumbhat S, Shankaran DR, Kim SJ, Gobi KV, Joshi V, Miura N. 2007. Surface plasmon resonance biosensor for dopamine using D3 dopamine receptor as a biorecognition molecule. Biosens Bioelectron 23: 421–427.
  • Ladero M, Santos A, Garcia JL, Carrascosa AV, Pessela BCC, Garcia-Ochoa F. 2002. Studies on the activity and stability of β galactosidase from Thermus sp. strain T2 and from Kluveromyces fragilis. Enzy Microb Technol 30: 392–405.
  • Ladero M, Ferrero R, Vian Santos A, Garcia-Ochoa F. 2005. Kinetic modeling of the thermal and pH inactivation of a thermostable β galactosidase from Thermus sp. strain T2. Enzy Microb Technol 37: 505–513.
  • Ladero M, Perez MT, Santos A, Garcia-Ochoa F. 2003. Hydrolysis of lactose by free and immobilized beta-galactosidase from Thermus sp. strain T2. Biotechnol Bioeng 81: 241–252.
  • Ladero M, Ruiz G, Pessela BCC, Vian A, Santos A, Garcia-Ochoa F. 2006. Thermal and pH inactivation of an immobilized thermostable β galactosidase from Thermus sp. strain T2: Comparison to the free enzyme. Biochem Eng J 31: 14–24.
  • Ladero M, Santos A, Garcia JL, Carrascosa AV, Pessela BCC, Garcia-Ochoa F. 2002. Studies on the activity and stability of β galactosidase from Thermus sp. strain T2 and from Kluveromyces fragilis. Enzy Microb Technol 30: 392–405.
  • Ladero M, Santos A, Garcia JL, Garcia-Ochoa F. 2001. Activity over lactose and ONPG of a genetically engineered β galactosidase from Escherichia coli in solution and immobilized kinetic modeling. Enzy Microb Technol 29: 181–193.
  • Ladero M, Santos A, García-Ochoa F. 2000. Kinetic modeling of lactose hydrolysis with an immobilized beta-galactosidase from Kluyveromyces fragilis. Enzyme Microb Technol 27: 583–592.
  • Lamas EM, Barros RM, Balcão VM, Malcata FX. 2001. Hydrolysis of whey proteins by proteases extracted from Cynara cardunculus and immobilized onto highly activated supports. Enzyme Microb Technol 28: 642–652.
  • Lamb SB, Stuckey DC. 2000. Enzyme immobilization on colloidal liquid aphrons (CLAs): the influence of system parameters on activity. Enzyme Microb Technol 26: 574–581.
  • Laridi R, Kheadr EE, Benech RO, Vuillermard JC, Lacroix C, Fliss I. 2003. Liposome encapsulated nisin Z: Optimization, stability and release during milk fermentation. Int Dairy J 13: 325–336.
  • Lazan H, Ng SY, Goh LY, Ali ZM. 2004. Papaya beta-galactosidase/galactanase isoforms in differential cell wall hydrolysis and fruit softening during ripening. Plant Physiol Biochem 42: 847–853.
  • Lee DH, Kang SG, Suh SG, Byun JK. 2003. Purification and characterization of a beta-galactosidase from peach (Prunus persica). Mol Cells 15: 68–74.
  • Lee MF, Krasinski SD. 1998. Human adult-onset lactase decline: an update. Nutr Rev 56: 1–8.
  • Lee YJ, Kim CS, Oh DK. 2004. Lactulose production by beta-galactosidase in permeabilized cells of Kluyveromyces lactis. Appl Microbiol Biotechnol 64: 787–793.
  • Levicky R, Horgan A. 2005. Physicochemical perspectives on DNA microarray and biosensor technologies. Trends Biotechnol 23: 143–149.
  • Li SC, Han JW, Chen KC, Chen CS. 2001. Purification and characterization of isoforms of beta-galactosidases in mung bean seedlings. Phytochemistry 57: 349–359.
  • Li X, Zhou QZK, Chen XD. 2007. Pilot-scale lactose hydrolysis using β galactosidase immobilized on cotton fabric. Chem Eng Process: Process Intensification 46: 497–500.
  • Liang JF, Li YT, Yang VC. 2000. Biomedical application of immobilized enzymes. J Pharm Sci 89: 979–990.
  • Liu X, Guan Y, Shen R, Liu H. 2005. Immobilization of lipase onto micron-size magnetic beads. J Chromatogr B Analyt Technol Biomed Life Sci 822: 91–97.
  • Lopez AC, Hernandez AC, Trejo AC, Lara FG, Lopez OP. 2002. Hydrolytic activity and ultrastructure in fruit skins from two prickly pear (Opuntia sp.) varieties during storage. J Agric Food Chem 50: 1681–1685.
  • Lopez-Gallego F, Betancor L, Hidalgo A, Alonso N, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R. 2005a. Preparation of a robust biocatalyst of D-amino acid oxidase on sepabeads supports using the glutaraldehyde crosslinking method. Enzy Microb Technol 37: 750–756.
  • López-Gallego F, Betancor L, Mateo C, Hidalgo A, Alonso-Morales N, Dellamora-Ortiz G, Guisán JM, Fernández-Lafuente R. 2005b. Enzyme stabilization by glutaraldehyde crosslinking of adsorbed proteins on aminated supports. J Biotechnol 119: 70–75.
  • Lu B, Smyth MR, O’Kennedy R. 1996. Oriented immobilization of antibodies and its applications in immunoassays and immunosensors. Analyst 121: 29R–32R.
  • Lukacheva LV, Zakemovskaya AA, Karyakina EE, Zorov IN, Sinitsyn AP, Sukhacheva MV, Netrusov AI, Karyakin AA. 2007. Determination of glucose and lactose in food products with the use of biosensors based on Berlin Blue. J Analyt Chem 62: 388–393.
  • Mahoney RR. 1997. Lactose: Enzymatic modification. In: Adv. Dairy Chem. (Fox, P. F. Ed.). New York: Chapman & Hall. 77–126.
  • Mammarella EJ, Rubiolo AC. 2005. Study of the deactivation of β galactosidase entrapped in alginate-carrageenan gels. J Molec Catal B: Enzym 34: 7–13.
  • Mammarella EJ, Rubiolo AC. 2006. Predicting the packed bed reactor performance with immobilized microbial lactase. Proc Biochem 41: 1627–1636.
  • Mandal M, Kundu S, Ghosh SK, Panigrahi S, Sau TK, Yusuf SM, Pal T. 2005. Magnetite nanoparticles with tunable gold or silver shell. J Colloid Interface Sci 286: 187–194.
  • Manta C, Ferraz, N, Betancor L, Antunes G, Batista-Viera F, Carlsson J, Caldwell K. 2003. Polyethylene glycol as a spacer for solid-phase enzyme immobilization. Enzy Microb Technol 33: 890–898.
  • Martins DB, de Souza CG Jr, Simões DA, de Morais MA Jr. 2002. The beta-galactosidase activity in Kluyveromyces marxianus CBS6556 decreases by high concentrations of galactose. Curr Microbiol 44: 379–382.
  • Mateo C, Abian O, Fernandez-Lafuente R, Guisan JM. 2000. Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment*. Enzyme Microb Technol 26: 509–515.
  • Mateo C, Abian O, Fernández-Lorente G, Pedroche J, Fernández-Lafuente R, Guisan JM, Tam A, Daminati M. 2002. Epoxy sepabeads: a novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment. Biotechnol Prog 18: 629–634.
  • Mateo C, Fernandez-Lorente G, Pessela BC, Vian A, Carrascosa AV, Garcia JL, Fernandez-Lafuente R, Guisan JM. 2001. Affinity chromatography of polyhistidine tagged enzymes. New dextran-coated immobilized metal ion affinity chromatography matrices for prevention of undesired multipoint adsorptions. J Chromatogr A 915: 97–106.
  • Mateo C, Palomo JM, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R. 2007a. Improvement of enzyme activity, stability and selectivity via immobilization techniques. A review. Enzy Microb Technol 40:1451–1463.
  • Mateo C, Grazu V, Palomo JM, Lopez-Gallego F, Fernandez-Lafuente R, Guisan JM. 2007b. Immobilization of enzymes on heterofunctional epoxy supports. Nat Protoc 2: 1022–1033.
  • Matioli G, Mores FFD, Zanin GM. 2003. Operated stability and kinetics of lactose hydrolysis by β galactosidase from Kluyveromyces fragilis. Scient Health Sci 25: 7–12.
  • Matto M, Husain Q. 2006. Entrapment of porous and stable concanavalin A-peroxidase complex into hybrid calcium alginate-pectin gel. J Chem Technol Biotechnol 81: 1316–1323.
  • Matto M, Husain Q. 2009. A novel calcium alginate-starch hybrid support for both surface immobilization and entrapment of bitter gourd (Momordica charantia) peroxidase. J Molec Catal B Enzym 57: 164–170.
  • McGee CM, Muray DR. 1986. Comparative studies of acid glycosidases from three legumes. Ann Botany 57: 179–190.
  • Milosavic N, Prodanovic R, Jovanovic S, Novakovic I, Vujcic Z. 2005. Preparation and characterization of two types of covalently immobilized amyloglucosidase. J Serb Chem Soc 70: 713–719.
  • Monnard PA. 2003. Liposome-entrapped polymerases as models for microscale/nanoscale bioreactors. J Membr Biol 191: 87–97.
  • Montalto M, Curigliano V, Santoro L, Vastola M, Cammarota G, Manna R, Gasbarrini A, Gasbarrini G. 2006. Management and treatment of lactose malabsorption. World J Gastroenterol 12: 187–191.
  • Montembault A, Viton C, Domard A. 2005. Rheometric study of the gelation of chitosan in a hydroalcoholic medium. Biomaterials 26: 1633–1643.
  • Mozhaev VV, Melik-Nubarov NS, Sergeeva MV, Sikrnis V, Martinek K. 1990. Strategy for stabilizing enzymes. I. Increasing stability of enzymes via their multipoint interaction with a support. Biocatalysis 3: 179–187.
  • Musthapa MS, Akhtar S, Khan AA, Husain Q. 2004. An economical, simple and high yield procedure for the immobilization/stabilization of peroxidases from turnip roots. J Sci Ind Res 63: 540–547.
  • Nagy Z, Kiss T, Szentirmai A, Biró S. 2001. Beta-galactosidase of Penicillium chrysogenum: production, purification, and characterization of the enzyme. Protein Expr Purif 21: 24–29.
  • Nakagawa T, Fujimoto Y, Uchino M, Miyaji T, Takano K, Tomizuka N. 2003. Isolation and characterization of psychrophiles producing cold-active beta-galactosidase. Lett Appl Microbiol 37: 154–157.
  • Nakagawa T, Ikehata R, Uchino M, Miyaji T, Takano K, Tomizuka N. 2006. Cold-active acid beta-galactosidase activity of isolated psychrophilic-basidiomycetous yeast Guehomyces pullulans. Microbiol Res 161: 75–79.
  • Nakkharat P, Haltrich D. 2006. Lactose hydrolysis and formation of galactooligosaccharides by a novel immobilized beta-galactosidase from the thermophilic fungus Talaromyces thermophilus. Appl Biochem Biotechnol 129-132: 215–225.
  • Neri DFM, Balcao VM, Carneiro-da-Cunha MG, Carvalho Jr LB, Teixeira JA. 2008. Immobilization of β galactosidase from Kluyveromyces lactis onto a polysiloxane-polyvinyl alcohol magnetic (mPOS-PVA) composite for lactose hydrolysis. Catal Comm 4: 234–239.
  • Nguyen TH, Splechtna B, Yamabhai M, Haltrich D, Peterbauer C. 2007. Cloning and expression of the beta-galactosidase genes from Lactobacillus reuteri in Escherichia coli. J Biotechnol 129: 581–591.
  • Nguyen TH, Splechtna B, Steinböck M, Kneifel W, Lettner HP, Kulbe KD, Haltrich D. 2006. Purification and characterization of two novel beta-galactosidases from Lactobacillus reuteri. J Agric Food Chem 54: 4989–4998.
  • Nilsson TK, Johansson CA. 2004. A novel method for diagnosis of adult hypolactasia by genotyping of the -13910 C/T polymorphism with Pyrosequencing technology. Scand J Gastroenterol 39: 287–290.
  • Nisnevitch M, Firer MA. 2001. The solid phase in affinity chromatography: strategies for antibody attachment. J Biochem Biophys Methods 49: 467–480.
  • Nogales JMR, Lopez AD. 2006. A novel approach to develop β galactosidase entrapped in liposomes in order to prevent an immediate hydrolysis of lactose in milk. Int Dairy J 16:354–360.
  • Noinville S, Revault M, Baron MH. 2002. Conformational changes of enzymes adsorbed at liquid-solid interface: relevance to enzymatic activity. Biopolymers 67: 323–326.
  • Norouzian D. 2003. Review article: enzyme immobilization: The state of art in biotechnology. Iranian J Biotechnol 1: 197–206.
  • Novalin S, Neuhaus W, Kulbe KD. 2005. A new innovative process to produce lactose-reduced skim milk. J Biotechnol 119: 212–218.
  • Numanoglu Y, Sungur S. 2004. β galactosidase from Kluyveromyces lactis cell disruption and enzyme immobilization using a cellulose-gelatin carrier system. Proc Biochem 39: 703–709.
  • Oliveira C, Teixeira JA, Lima N, Da Silva NA, Domingues L. 2007. Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger beta-galactosidase production. J Biosci Bioeng 103: 318–324.
  • Ovsejevi K, Grazu V, Cuadra K, Batista-Viera F. 2004. Enzyme reduction on solid phase as a tool for the reversible immobilization of yeast β galactosidase onto a thiol-reactive support. Enzy Microb Technol 35: 203–209.
  • Ozbek B, Ulgen K. 2000. The stability of enzymes after sonication. Proc Biochem 35: 1037–1043.
  • Ozdural AR, Tanyolac D, Boyac IH, Mutlu M, Webb C. 2003. Determination of apparent kinetic parameters for competitive product inhibition in packed-bed immobilized reactor. Biochem Eng J 14:27–36.
  • Pakula TM, Salonen K, Uusitalo J, Penttilä M. 2005. The effect of specific growth rate on protein synthesis and secretion in the filamentous fungus Trichoderma reesei. Microbiology (Reading, Engl.) 151: 135–143.
  • Palomo JM, Segura RL, Fernandez-Lorente G, Guisan JM, Fernandez-Lafuente R. 2007. Glutaraldehyde modification of lipases adsorbed on animated supports: A simple way to improve their behavior as enantioselective biocatalyst. Enzy Microb Technol 40: 704–707.
  • Panesar PS, Panesar PS, Singh RS, Kennedy JF, Kumar H. 2006. Microbial production, immobilization and applications of β-D-galactosidase. J Chem Technol Biotechnol 81: 530–543.
  • Panesar R, Panesar PS, Singh RS, Kennedy JF, Bera MB. 2007. Production of lactose-hydrolyzed milk using ethanol permeabilized yeast cells. Food Chem 101: 786–790.
  • Parascandola P, Branduardi P, De Alteriis E. 2006. PVA-gel Lentikats (R) as an effective matrix for yeast strain immobilization aimed at heterologous protein production. Enzym Microb Technol 38: 184–189.
  • Patel PD. 2002. Biosensors for measurement of analytes implicated in food safety: A review. Trends Analyt Chem 21: 96–115.
  • Pedroche J, Yust MM, Mateo C, Fernandez-Lafuente R, Giron-Calle J, Alaiz M, Vioque J, Guisan JM, Millan F. 2007. Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: Correlation between enzyme-support linkages and thermal stability. Enzym Microb Technol 40: 1161–1167.
  • Peppas NA, Bures P, Leobandung W, Ichikawa H. 2000. Hydrogels in pharmaceutical formulations. Eur J Pharm Biopharm 50: 27–46.
  • Pessela BCC, Fuentes M, Mateo C, Munilla R, Carrascosa AV, Fernandez-Lafuente R, Guisan JM. 2006. Purification and very strong reversible immobilization of large proteins on anionic exchangers by controlling the support and the immobilization conditions. Enzym Microb Technol 39: 909–915.
  • Pessela BC, Munilla R, Betancor L, Fuentes M, Carrascosa AV, Vian A, Fernandez-Lafuente R, Guisán JM. 2004a. Ion exchange using poorly activated supports, an easy way for purification of large proteins. J Chromatogr A 1034: 155–159.
  • Pessela BC, Torres R, Fuentes M, Mateo C, Munilla R, Vian A, Carrascosa AV, Garcia JL, Guisán JM, Fernandez-Lafuente R. 2004b. Selective and mild adsorption of large proteins on lowly activated immobilized metal ion affinity chromatography matrices. Purification of multimeric thermophilic enzymes overexpressed in Escherichia coli. J Chromatogr A 1055: 93–98.
  • Pessela BCC, Mateo C, Filho M, Carrascosa A, Lafuente RF, Guisan JM. 2007. Selective adsorption of large proteins on highly activated IMAC supports in the presence of high imidazole concentrations: Purification, reversible immobilization and stabilization of thermophilic α and β galactosidase. Enzym Microb Technol 40: 242–248.
  • Pessela BCC, Mateo C, Fuentes M, Vian A, Garcia JL, Carrascosa AV, Guisan JM, Lafuente RF. 2003. The immobilization of a thermophilic β galactosidase on sepabeads supports decreases product inhibition complete hydrolysis of lactose in dairy products. Enzym Microb Technol 33: 199–205.
  • Picard C, Fioramonti J, Francois A, Robinson T, Neant F, Matuchansky C. 2005. Review article: bifidobacteria as probiotic agents – physiological effects and clinical benefits. Aliment Pharmacol Ther 22: 495–512.
  • Pierre AC. 2004. The sol-gel encapsulation of enzymes. Biocatal Biotrans 22: 145–170.
  • Pivarnik LF, Senecal AG, Rand AG. 1995. Hydrolytic and transgalactosylic activities of commercial beta-galactosidase (lactase) in food processing. Adv Food Nutr Res 38: 1–102.
  • Plantz BA, Nickerson K, Kachman SD, Schlegel VL. 2007. Evaluation of metals in a defined medium for Pichia pastoris expressing recombinant beta-galactosidase. Biotechnol Prog 23: 687–692.
  • Porekar VG, Menart V. 2001. Perspectives of immobilized-metal affinity chromatography. J Biochem Biophys Meth 49: 335–360.
  • Portaccio M, Stellato S, Rossi S, Bencivenga U, Eldin MSM, Gaeta FS, Mita DG. 1998. Galactose competitive inhibition of β galactosidase (Aspergillus oryzae) immobilized on chitosan and nylon supports. Enzym Microb Technol 23: 101–106.
  • Portugall IL, Montermann E, Kremer A, Reske-Kunz AB, Sudowe S. 2004. Prevention of long-term IgE antibody production by gene gun-mediated DNA vaccination. J Allergy Clin Immunol 114: 951–957.
  • Prata-Vidal M, Bouhallab S, Henry G, Aimar P. 2001. An experimental study of caseinomacropeptide hydrolysis by trypsin in a continuous membrane reactor. Biochem Eng J 8: 195–202.
  • Prazeres DMF, Cabral JMS. 1994. Enzymatic membrane bioreactors and their applications. Enzym Microb Technol 16: 738–750.
  • Pribila BA, Hertzler SR, Martin BR, Weaver CM, Savaiano DA. 2000. Improved lactose digestion and intolerance among African-American adolescent girls fed a dairy-rich diet. J Am Diet Assoc 100: 524–528; quiz 529.
  • Prieto CA, Guadix A, Gonzalez-Tello P, Guadix EM. 2007. A cyclic batch membrane reactor for the hydrolysis of whey protein. J Food Eng 78: 257–265.
  • Ranwala AP, Suematsu C, Masuda H. 1992. The role of beta-galactosidases in the modification of cell wall components during muskmelon fruit ripening. Plant Physiol 100: 1318–1325.
  • Rasinperä H, Savilahti E, Enattah NS, Kuokkanen M, Tötterman N, Lindahl H, Järvelä I, Kolho KL. 2004. A genetic test which can be used to diagnose adult-type hypolactasia in children. Gut 53: 1571–1576.
  • Rebros M, Rosenberg M, Mlichova Z, Kristofikova, L, Paluch M. 2006. A simple entrapment of glucoamylase into LentiKats as an efficient catalyst for maltodextrin hydrolysis. Enzym Microb Technol 39: 800–804.
  • Rech R, Ayub MAZ. 2007. Simplified feeding strategies for fed-batch cultivation of Kluyveromyces marxianus in cheese whey. Proc Biochem 42: 873–877.
  • Rejikumar S, Surekha D. 2001. Hydrolysis of lactose and milk whey using a fixed-bed reactor containing β galactosidase covalently bound onto chitosan and cross-linked poly (vinyl alcohol). Int J Food Sci Technol 36: 91–98.
  • Ribeiro O, Gombert AK, Teixeira JA, Domingues L. 2007. Application of the Cre-loxP system for multiple gene disruption in the yeast Kluyveromyces marxianus. J Biotechnol 131: 20–26.
  • Richmond ML, Gray JI, Stine CM. 1981. β galactosidase: review of recent research related to technological application, nutritional concerns, and immobilization. J Dairy Sci 64: 1759–1771.
  • Ridefelt P, Håkansson LD. 2005. Lactose intolerance: lactose tolerance test versus genotyping. Scand J Gastroenterol 40: 822–826.
  • Rios GM, Belleville MP, Paolucci D, Sanchez J. 2004. Progress in enzymatic membrane reactors-A review. J Memb Sci 242: 189–196.
  • Rizkalla SW, Luo J, Kabir M, Chevalier A, Pacher N, Slama G. 2000. Chronic consumption of fresh but not heated yogurt improves breath-hydrogen status and short-chain fatty acid profiles: a controlled study in healthy men with or without lactose maldigestion. Am J Clin Nutr 72: 1474–1479.
  • Rodriguez-Nogales JM, Delgadillo-Lopez A. 2005. Stability and catalytic kinetics of microencapsulated β galactosidase in liposomes prepared by the dehydration–rehydration method. J Molec Catal B Enzym 33: 15–21.
  • Rodriguez-Nogales JM, Delgadillo-Lopez A. 2006. A novel approach to develop β galactosidase entrapped in liposomes in order to prevent an immediate hydrolysis of lactose in milk. Int Dairy J 16: 354–360.
  • Rogers KR. 2006. Recent advances in biosensor techniques for environmental monitoring. Anal Chim Acta 568: 222–231.
  • Roldan CR, Galan LP, Roa J, Castro A, Pietro AD, Roncero MIG, Hera C. 2008. The Fusarium oxysporum sti35 gene functions in thiamine biosynthesis and oxidative stress response. Fungal Genet Biol 45: 6–16.
  • Ross GS, Wegrzyn T, MacRae EA, Redgwell RJ. 1994. Apple beta-galactosidase. Activity against cell wall polysaccharides and characterization of a related cDNA clone. Plant Physiol 106: 521–528.
  • Rossi A, Morana A, Lernia ID, Di tombrino A, De Rosa M. 1999. Immobilization of enzymes on spongy polyvinyl alcohol cryogels: the example of beta-galactosidase from Aspergillus oryzae. Ital J Biochem 48: 91–97.
  • Roy I, Gupta MN. 2003. Lactose hydrolysis by lactozymTM immobilized on cellulose beads in batch and fluidized bed modes. Proc Biochem 39: 325–332.
  • Salzman G, Tadmor R, Guzy S, Sideman S, Lotan N. 1999. Hollow-fibre enzymic reactors for a two substrate process: analytical modelling and numerical simulations. Chem Eng Proc 38: 289–299.
  • Sanjay G, Sugunan S. 2005. Invertase immobilized on montmorillonite: Reusability enhancement and reduction in leaching. Catal Comm 6: 81–86.
  • Sarney DB, Hale C, Frankel G, Vulfson EN. 2000. A novel approach to the recovery of biologically active oligosaccharides from milk using a combination of enzymatic treatment and nanofiltration. Biotechnol Bioeng 69: 461–467.
  • Satar R, Matto M, Husain Q. 2008. Studies on calcium alginate-pectin gel entrapped concanavalin A-bitter gourd (Momordica charantia) peroxidase complex. J Sci Ind Res 67: 609–615.
  • Sener N, Apar DK, Ozbek B. 2006. A modeling study on lactose hydrolysis and β galactosidase stability under sonication. Proc Biochem 41:1493–1500.
  • Serio MD, Maturo C, Alteriis ED, Parascandola P, Tesser R, and Santacesaria E. 2003. Lactose hydrolysis by immobilized β galactosidase: The effect of the supports and the kinetics. Catal Today 79–80: 333–339.
  • Sezgintürk MK, Dinçkaya E. 2008. Beta-galactosidase monitoring by a biosensor based on Clark electrode: its optimization, characterization and application. Biosens Bioelectron 23: 1799–1804.
  • Shankaran DR, Gobi KV, Sakai T, Matsumoto K, Toko K, Miura N. 2005. Surface plasmon resonance immunosensor for highly sensitive detection of 2,4,6-trinitrotoluene. Biosens Bioelectron 20: 1750–1756.
  • Sharma SK, Singhal R, Malhotra BD, Sehgal N, Kumar A. 2004. Lactose biosensor based on Langmuir-Blodgett films of poly(3-hexyl thiophene). Biosens Bioelectron 20: 651–657.
  • Shchipunov YA, Karpenko TY, Bakunina IY, Burtseva YV, Zvyagintseva TN. 2004. A new precursor for the immobilization of enzymes inside sol-gel-derived hybrid silica nanocomposites containing polysaccharides. J Biochem Biophys Methods 58: 25–38.
  • Sheu DC, Li SY, Duan KJ, Chen CW. 1998. Production of galacto-oligosaccharides by β galactosidase immobilized on glutaraldehyde treated chitosan beads. Biotechnol Techn 12: 273–276.
  • Shibatane T, Omori K, Akatsuka H, Kawai E, Matsumae H. 2000. Enzymatic resolution of diltiazem intermediate by Serratia marcescens lipase: Molecular mechanism of lipase secretion and its industrial application. J Molec Catal B: Enzym 10: 141–149.
  • Sinha R, Radha C, Prakash J, Kaul P. 2007. Whey protein hydrolysate: Functional properties, nutritional quality and utilization of beverage formulation. Food Chem 101: 1484–1491.
  • Slepak T, Tang M, Addo F, Lai K. 2005. Intracellular galactose-1-phosphate accumulation leads to environmental stress response in yeast model. Mol Genet Metab 86: 360–371.
  • Smith DL, Gross KC. 2000. A family of at least seven beta-galactosidase genes is expressed during tomato fruit development. Plant Physiol 123: 1173–1183.
  • Smith DL, Starrett DA, Gross KC. 1998. A gene coding for tomato fruit beta-galactosidase II is expressed during fruit ripening. Cloning, characterization, and expression pattern. Plant Physiol 117: 417–423.
  • Sousa R Jr., Lopes GP, Tardioli PW, Giordano RLC, Almeida PIF, Giordano RC. 2004. Kinetic model for whey protein hydrolysis by alcalase multipoint-immobilized on agarose gel particles. Brazilian J Chem Eng 21: 147–153.
  • Staby A, Sand MB, Hansen RG, Jacobsen JH, Andersen LA, Gerstenberg M, Bruus UK, Jensen IH. 2005. Comparison of chromatographic ion-exchange resins IV. Strong and weak cation-exchange resins and heparin resins. J Chromatogr A 1069: 65–77.
  • Stefano DM, Malservisi S, Veneto G, Ferrieri A, Corazza GR. 2000. Rifaximin versus chlortetracycline in the short-term treatment of small intestinal bacterial overgrowth. Aliment Pharmacol Ther 14: 551–556.
  • Stenekes RJ, Loebis AE, Fernandes CM, Crommelin DJ, Hennink WE. 2000. Controlled release of liposomes from biodegradable dextran microspheres: a novel delivery concept. Pharm Res 17: 690–695.
  • Suarez FL, Savaiano DA, Levitt MD. 1995. Review article: the treatment of lactose intolerance. Aliment Pharmacol Ther 9: 589–597.
  • Sung HW, Chen CN, Liang HF, Hong MH. 2003. A natural compound (reuterin) produced by Lactobacillus reuteri for biological-tissue fixation. Biomaterials 24: 1335–1347.
  • Swallow DM. 2003. Genetics of lactase persistence and lactose intolerance. Annu Rev Genet 37: 197–219.
  • Szczodrak J. 2000. Hydrolysis of lactose in whey permeate by immobilized β galactosidase from Kluyveromyces fragilis. J Molec Cataly B: Enzym 10: 631–637.
  • Tanaka A, Kawamoto T. 1999. Cell and enzyme immobilization. In: Manual of Industrial Microbiology and Biotechnology. (Demain, A. L., Davies, J. E., Atlas, R. M., Cohen, G., Hershberger, C. L., Hu, W. S., Sherman, D. H., Willson, R. C., Wu, J. H. D. Eds.). Washington DC: ASM Press. 94–102.
  • Tanaka Y, Kagamiishi A, Kiuchi A, Horiuchi T. 1975. Purification and properties of beta-galactosidase from Aspergillus oryzae. J Biochem 77: 241–247.
  • Tanriseven A, Dogan S. 2002. A novel method for the immobilization of β galactosidase. Proc Biochem 38: 27–30.
  • Taqieddin E, Amiji M. 2004. Enzyme immobilization in novel alginate-chitosan core-shell microcapsules. Biomaterials 25: 1937–1945.
  • Taqieddin E, Lee C, Amiji M. 2002. Perm-selective chitosan-alginate hybrid microcapsules for enzyme immobilization technology. Pharm Eng 22: 1–3.
  • Tardioli PW, Sousa R Jr., Giordano RC, Giordano RLC. 2005. Kinetic model of the hydrolysis of polypeptides catalyzed by alcalase immobilized on 10% glyoxyl-agarose. Enzy Microb Technol 36: 555–564.
  • Tateishi A, Inoue H, Yamaki S. 2001. Fluctuations in activities of three β galactosidase isoforms from ripening avocado (Persea americana) fruit and their different activities against its cell wall polysaccharides as substrates. J Japan Soc Horticult Sci 70: 586–592.
  • Tello-Solís SR, Jiménez-Guzmán J, Sarabia-Leos C, Gómez-Ruíz L, Cruz-Guerrero AE, Rodríguez-Serrano GM, García-Garibay M. 2005. Determination of the secondary structure of Kluyveromyces lactis beta-galactosidase by circular dichroism and its structure-activity relationship as a function of the pH. J Agric Food Chem 53: 10200–10204.
  • Thompkinson DK, Kharb S. 2007. Aspects of infant food formulation. Compreh Rev Food Sci Food Saf 6: 79–102.
  • Tischer W, Kasche V. 1999. Immobilized enzymes: crystals or carriers? Trends Biotechnol 17: 326–335.
  • Tischer W, Wedekind F. 1999. Immobilized enzyme: methods and applications Biocatalysis 200: 95–126.
  • Tong XD, Xue B, Sun Y. 2001. A novel magnetic affinity support for protein adsorption and purification. Biotechnol Prog 17: 134–139.
  • Torres R, Pessela BC, Mateo C, Ortiz C, Fuentes M, Guisan JM, Fernandez-Lafuente R. 2004. Reversible immobilization of glucoamylase by ionic adsorption on sepabeads coated with polyethyleneimine. Biotechnol Prog 20: 1297–1300.
  • Tragardh G. 1991. Membrane application in the food industry. Polym J 23: 521–529.
  • Trainotti L, Spinello R, Piovan A, Spolaore S, Casadoro G. 2001. beta-Galactosidases with a lectin-like domain are expressed in strawberry. J Exp Bot 52: 1635–1645.
  • Tu W, Sun S, Nu S, Li X. 1999. Immobilization of β galactosidase from Cicer arietinum (gram chicken bean) and its catalytic actions. Food Chem 64: 495–500.
  • Turkiewicz M, Kur J, Bialkowska A, Cieslinski H, Kalinowska H, Bielecki S. 2003. Antarctic marine bacterium Pseudoalteromonas sp. 22b as a source of cold-adapted beta-galactosidase. Biomol Eng 20: 317–324.
  • Turková J. 1999. Oriented immobilization of biologically active proteins as a tool for revealing protein interactions and function. J Chromatogr B Biomed Sci Appl 722: 11–31.
  • Uludag H, De Vos P, Tresco PA. 2000. Technology of mammalian cell encapsulation. Adv Drug Deliv Rev 42: 29–64.
  • Vasilijevic T, Jelen P. 2001. Production of β galactosidase for lactose hydrolysis in milk and dairy products using thermophilic lactic acid bacteria. Innov Food Sci Emerg Technol 2: 75–85.
  • Vasiljevic T, Jelen P. 2002. Lactose hydrolysis in milk as affected by neutralizers used for the preparation of crude β galactosidase extracts from Lactobacillus bulgaricus 11842. Innov Food Sci Emerg Technol 3: 175–184.
  • Vasiljevic T, Jelen P. 2003. Retention of β galactosidase activity in crude cellular extracts from Lactobacillus delbrueckii sp. bulgaricus 11842 upon drying. Int J Dairy Technol 56: 111–116.
  • Veronese FM, Mammucari C, Schiavon F, Schiavon O, Lora S, Secundo F, Chilin A, Guiotto A. 2001. Pegylated enzyme entrapped in poly(vinyl alcohol) hydrogel for biocatalytic application. Farmaco 56: 541–547.
  • Vinderola CG, Reinheimer JA. 2003. Lactic acid starter and probiotic bacteria: A comparative in vitro study of probiotic characteristics and biological barrier resistance. Food Res Int 36: 895–904.
  • Vonk RJ, Lin Y, Koetse HA, Huang C, Zeng G, Elzinga H, Antoine J, Stellaard F. 2000. Lactose (mal)digestion evaluated by the 13C-lactose digestion test. Eur J Clin Invest 30: 140–146.
  • Walde P, Ichikawa S. 2001. Enzymes inside lipid vesicles: preparation, reactivity and applications. Biomol Eng 18: 143–177.
  • Wang D, Sakakibara M. 1997. Lactose hydrolysis and beta-galactosidase activity in sonicated fermentation with Lactobacillus strains. Ultrason Sonochem 4: 255–261.
  • Wang Y, Harvey CB, Hollox EJ, Phillips AD, Poulter M, Clay P, Walker-Smith JA, Swallow DM. 1998. The genetically programmed down-regulation of lactase in children. Gastroenterology 114: 1230–1236.
  • Wilson J. 2005. Milk intolerance: lactose intolerance and cow’s milk protein allergy. Newborn Infant Nursing Rev 5: 203–207.
  • Wilson L, Illanes A, Pessela BC, Abian O, Fernández-Lafuente R, Guisán JM. 2004. Encapsulation of crosslinked penicillin G acylase aggregates in lentikats: evaluation of a novel biocatalyst in organic media. Biotechnol Bioeng 86: 558–562.
  • Won K, Kim S, Kim KJ, Park HW, Moon SJ. 2005. Optimization of lipase entrapment in Ca-alginate gel beads. Proc Biochem 40: 2149–2154.
  • Wu JC, Wong YK, Chang KW, Tay CY, Chow Y, Talukder M, Choi W. 2007. Immobilization of Mucor javanicus lipase by entrapping in alginate-silica hybrid gel beads with simultaneous cross-linking with glutaraldehyde. J Biocatal Biotrans 25: 459–463.
  • Wu JM, Chen ZX, Ran DL. 2002. Cross-linking chitosan immobilized on silica gel as a matrix for preparation of affinity chromatographic material. Clin J Analyt Chem 30: 1063–1067.
  • Xi F, Wu J, Jia Z, Lin X. 2005. Preparation and characterization of trypsin immobilized on silica gel supported macroporous chitosan bead. Proc Biochem 40: 2833–2840.
  • Yang BL, Shinoda N, Goto S. 1998. Hydrolysis of o-nitrophenyl-β-D-galactoside by an enzyme immobilized on perflurocarbon in a tapered column reactor. Coll SurfB: Biointerf 10: 127–135.
  • Yemini M, Reches M, Gazit E, Rishpon J. 2005. Peptide nanotube-modified electrodes for enzyme-biosensor applications. Anal Chem 77: 5155–5159.
  • Zhang S, Gao S, Gao G. 2009. Immobilization of β galactosidase onto magnetic beads. Appl Biochem Biotechnol (in press).
  • Zhou QZ, Chen XD, Li X. 2003. Kinetics of lactose hydrolysis by beta-galactosidase of Kluyveromyces lactis immobilized on cotton fabric. Biotechnol Bioeng 81: 127–133.
  • Zhou QZK, Chen XD. 2001a. Effect of temperature and pH on the catalytic activity of the immobilized β galactosidase from Kluyveromyces lactis. Biochem Eng J 9: 33–40.
  • Zhou QZK, Chen XD. 2001b. Immobilization of β galactosidase on graphite surface by glutaraldehyde. J Food Eng 48: 69–74.

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