638
Views
37
CrossRef citations to date
0
Altmetric
Review Article

Recombinant lectins: an array of tailor-made glycan-interaction biosynthetic tools

, &
Pages 66-80 | Received 04 Oct 2011, Accepted 24 Feb 2012, Published online: 24 Apr 2012

References

  • Adar R, Streicher H, Rozenblatt S, Sharon N. 1997. Synthesis of soybean agglutinin in bacterial and mammalian cells. Eur J Biochem 249: 684–689.
  • Al Atalah B, Fouquaert E, Vanderschaeghe D, Proost P, Balzarini J, Smith DF, Rougé P, Lasanajak Y, Callewaert N, Van Damme EJ. 2011. Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris. FEBS J 278: 2064–2079.
  • Amano K, Takase M, Ando A, Nagata Y. 2003. Production of functional lectin in Pichia pastoris directed by cloned cDNA from Aleuria aurantia. Biosci Biotechnol Biochem 67: 2277–2279.
  • Baumgartner P, Harper K, Raemaekers RJ, Durieux A, Gatehouse AM, Davies HV, Taylor MA. 2003. Large-scale production and purification of recombinant Galanthus nivalis agglutinin (GNA) expressed in the methylotrophic yeast Pichia pastoris. Biotechnol Lett 25: 1281–1285.
  • Baumgartner P, Raemaekers RJ, Durieux A, Gatehouse A, Davies H, Taylor M. 2002. Large-scale production, purification, and characterisation of recombinant Phaseolus vulgaris phytohemagglutinin E-form expressed in the methylotrophic yeast Pichia pastoris. Protein Expr Purif 26: 394–405.
  • Bezerra WM, Carvalho CP, Moreira Rde A, Grangeiro TB. 2006. Establishment of a heterologous system for the expression of Canavalia brasiliensis lectin: a model for the study of protein splicing. Genet Mol Res 5: 216–223.
  • Blonski K, Schumacher U, Burkholder I, Edler L, Nikbakht H, Boeters I, Peters A, Kugler C, Horny HP, Langer M, Wilhelm-Ogunbiyi K, Witthohn K, Laack E. 2005. Binding of recombinant mistletoe lectin (aviscumine) to resected human adenocarcinoma of the lung. Anticancer Res 25: 3303–3307.
  • Carvalho CP, Rocha CS, Nepomuceno DR, Oliveira JT, Grangeiro TB. 2008. Expression of a Canavalia brasiliensis lectin (ConBr) precursor in Pichia pastoris. Protein Pept Lett 15: 327–332.
  • da Silveira Carvalho CP, Correia TO, Ribeiro SM, Silva IC, de Oliveira JT, Grangeiro TB. 2006. Expression of the Canavalia brasiliensis lectin (ConBr) in tobacco plants. Protein Pept Lett 13: 1045–1049.
  • Chang WC, Hartshorn KL, White MR, Moyo P, Michelow IC, Koziel H, Kinane BT, Schmidt EV, Fujita T, Takahashi K. 2011. Recombinant chimeric lectins consisting of mannose-binding lectin and L-ficolin are potent inhibitors of influenza A virus compared with mannose-binding lectin. Biochem Pharmacol 81: 388–395.
  • Chao Q, Etzler ME. 1994. Incorrect targeting of plant vacuolar lectins in yeast. J Biol Chem 269: 20866–20871.
  • Chrispeels MJ, Raikhel NV. 1991. Lectins, lectin genes, and their role in plant defense. Plant Cell 3: 1–9.
  • Cregg JM, Cereghino JL, Shi J, Higgins DR. 2000. Recombinant protein expression in Pichia pastoris. Mol Biotechnol 16: 23–52.
  • Cregg JM, Vedvick TS, Raschke WC. 1993. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology (NY) 11: 905–910.
  • daSilva LL, de Molfetta-Machado JB, Panunto-Castelo A, Denecke J, Goldman GH, Roque-Barreira MC, Goldman MH. 2005. cDNA cloning and functional expression of KM+, the mannose-binding lectin from Artocarpus integrifolia seeds. Biochim Biophys Acta 1726: 251–260.
  • De Hoff PL, Brill LM, Hirsch AM. 2009. Plant lectins: the ties that bind in root symbiosis and plant defense. Mol Genet Genomics 282: 1–15.
  • De Mejía EG, Prisecaru VI. 2005. Lectins as bioactive plant proteins: a potential in cancer treatment. Crit Rev Food Sci Nutr 45: 425–445.
  • Demain AL, Vaishnav P. 2009. Production of recombinant proteins by microbes and higher organisms. Biotechnol Adv 27: 297–306.
  • Eda S, Suzuki Y, Kawai T, Ohtani K, Kase T, Sakamoto T, Wakamiya N. 1998. Characterization of truncated human mannan-binding protein (MBP) expressed in Escherichia coli. Biosci Biotechnol Biochem 62: 1326–1331.
  • Ferrini JB, Martin M, Taupiac MP, Beaumelle B. 1995. Expression of functional ricin B chain using the baculovirus system. Eur J Biochem 233: 772–777.
  • Fitches E, Audsley N, Gatehouse JA, Edwards JP. 2002. Fusion proteins containing neuropeptides as novel insect contol agents: snowdrop lectin delivers fused allatostatin to insect haemolymph following oral ingestion. Insect Biochem Mol Biol 32: 1653–1661.
  • Fitches E, Edwards MG, Mee C, Grishin E, Gatehouse AM, Edwards JP, Gatehouse JA. 2004. Fusion proteins containing insect-specific toxins as pest control agents: snowdrop lectin delivers fused insecticidal spider venom toxin to insect haemolymph following oral ingestion. J Insect Physiol 50: 61–71.
  • Fitches E, Philip J, Hinchliffe G, Vercruysse L, Chougule N, Gatehouse JA. 2008a. An evaluation of garlic lectin as an alternative carrier domain for insecticidal fusion proteins. Insect Sci 15: 483–495.
  • Fitches E, Wiles D, Douglas AE, Hinchliffe G, Audsley N, Gatehouse JA. 2008b. The insecticidal activity of recombinant garlic lectins towards aphids. Insect Biochem Mol Biol 38: 905–915.
  • Fitches EC, Bell HA, Powell ME, Back E, Sargiotti C, Weaver RJ, Gatehouse JA. 2010. Insecticidal activity of scorpion toxin (ButaIT) and snowdrop lectin (GNA) containing fusion proteins towards pest species of different orders. Pest Manag Sci 66: 74–83.
  • Fouquaert E, Smith DF, Peumans WJ, Proost P, Balzarini J, Savvides SN, Damme EJ. 2009. Related lectins from snowdrop and maize differ in their carbohydrate-binding specificity. Biochem Biophys Res Commun 380: 260–265.
  • Frankel A, Roberts H, Gulick H, Afrin L, Vesely J, Willingham M. 1994. Expression of ricin B chain in Spodoptera frugiperda. Biochem J 303 (Pt 3): 787–794.
  • Gemeiner P, Mislovicová D, Tkác J, Svitel J, Pätoprstý V, Hrabárová E, Kogan G, Kozár T. 2009. Lectinomics II. A highway to biomedical/clinical diagnostics. Biotechnol Adv 27: 1–15.
  • Ghazarian H, Idoni B, Oppenheimer SB. 2011. A glycobiology review: carbohydrates, lectins and implications in cancer therapeutics. Acta Histochem 113: 236–247.
  • Giomarelli B, Schumacher KM, Taylor TE, Sowder RC 2nd, Hartley JL, McMahon JB, Mori T. 2006. Recombinant production of anti-HIV protein, griffithsin, by auto-induction in a fermentor culture. Protein Expr Purif 47: 194–202.
  • Gupta G, Surolia A, Sampathkumar SG. 2010. Lectin microarrays for glycomic analysis. OMICS 14: 419–436.
  • Hansen S, Holm D, Moeller V, Vitved L, Bendixen C, Reid KB, Skjoedt K, Holmskov U. 2002. CL-46, a novel collectin highly expressed in bovine thymus and liver. J Immunol 169: 5726–5734.
  • Hilder VA, Powell KS, Gatehouse AMR, Gatehouse JA, Gatehouse LN, Shi Y, et al. 1995. Expression of snowdrop lectin in transgenic tobacco plants results in added protection against aphids. Transgenic Res 4: 18–25.
  • Hsu KL, Gildersleeve JC, Mahal LK. 2008. A simple strategy for the creation of a recombinant lectin microarray. Mol Biosyst 4: 654–662.
  • Iijima N, Amano K, Ando A, Nagata Y. 2003. Production of fruiting-body lectins of Pleurotus cornucopiae in methylotrophic yeast Pichia pastoris. J Biosci Bioeng 95: 416–418.
  • Jagtap UB, Bapat VA. 2010. Artocarpus: a review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 129: 142–166.
  • Jiang JF, Han Y, Xing LJ, Xu YY, Xu ZH, Chong K. 2006. Cloning and expression of a novel cDNA encoding a mannose-specific jacalin-related lectin from Oryza sativa. Toxicon 47: 133–139.
  • Kakiuchi M, Okino N, Sueyoshi N, Ichinose S, Omori A, Kawabata S, Yamaguchi K, Ito M. 2002. Purification, characterization, and cDNA cloning of alpha-N-acetylgalactosamine-specific lectin from starfish, Asterina pectinifera. Glycobiology 12: 85–94.
  • Kim YH, Woloshuk CP, Cho EH, Bae JM, Song YS, Huh GH. 2007. Cloning and functional expression of the gene encoding an inhibitor against Aspergillus flavus alpha-amylase, a novel seed lectin from Lablab purpureus (Dolichos lablab). Plant Cell Rep 26: 395–405.
  • Kong P, Wang L, Zhang H, Song X, Zhou Z, Yang J, Qiu L, Wang L, Song L. 2011. A novel C-type lectin from bay scallop Argopecten irradians (AiCTL-7) agglutinating fungi with mannose specificity. Fish Shellfish Immunol 30: 836–844.
  • Kreitman RJ. 2006. Immunotoxins for targeted cancer therapy. AAPS J 8: E532–E551.
  • Kuhlman M, Joiner K, Ezekowitz RA. 1989. The human mannose-binding protein functions as an opsonin. J Exp Med 169: 1733–1745.
  • Kumar M, Behera AK, Kumar S, Srinivas VR, Das HR, Surolia A, Das RH. 1999. Expression, purification and characterization of peanut (Arachis hypogaea) agglutinin (PNA) from baculovirus infected insect cells. Biosci Rep 19: 227–234.
  • Kuo TH, Chuang SC, Chang SY, Liang PH. 2006. Ligand specificities and structural requirements of two Tachypleus plasma lectins for bacterial trapping. Biochem J 393: 757–766.
  • Kurata H, Cheng HM, Kozutsumi Y, Yokota Y, Kawasaki T. 1993. Role of the collagen-like domain of the human serum mannan-binding protein in the activation of complement and the secretion of this lectin. Biochem Biophys Res Commun 191: 1204–1210.
  • Lam SK, Ng TB. 2011. Lectins: production and practical applications. Appl Microbiol Biotechnol 89: 45–55.
  • Lannoo N, Van Damme EJ. 2010. Nucleocytoplasmic plant lectins. Biochim Biophys Acta 1800: 190–201.
  • Lannoo N, Vervecken W, Proost P, Rougé P, Van Damme EJ. 2007. Expression of the nucleocytoplasmic tobacco lectin in the yeast Pichia pastoris. Protein Expr Purif 53: 275–282.
  • Li CY, Luo P, Liu JJ, Wang EQ, Li WW, Ding ZH, et al. 2011a. Recombinant expression of Polygonatum cyrtonema lectin with anti-viral, apoptosis-inducing activities and preliminary crystallization. Process Biochem 46: 533–542.
  • Li JD, Xu Q, Wang KY. 2001. Functional expression of a galactose-binding C-type lectin in Pichia pastoris. Protein Pept Lett 8: 217–224.
  • Li Y, Liao X, Chen G, Yap Y, Zhang X. 2011b. Cloning, expression and purification of Microcystis viridis lectin in Escherichia coli. Mol Biotechnol 47: 105–110.
  • Li Y, Zhang X. 2010. Recombinant Microcystis viridis lectin as a potential anticancer agent. Pharmazie 65: 922–923.
  • Lindstrom JT, Vodkin LO, Harding RW, Goeken RM. 1990. Expression of soybean lectin gene deletions in tobacco. Dev Genet 11: 160–167.
  • Ling LJ, Yang YZ, Bi YR. 2010. Expression and characterization of two domains of Pinellia ternata agglutinin (PTA), a plant agglutinin from Pinellia ternata with antifungal activity. World J Microbiol Biotechnol 26: 545–554.
  • Liu B, Bian HJ, Bao JK. 2010. Plant lectins: potential antineoplastic drugs from bench to clinic. Cancer Lett 287: 1–12.
  • Longstaff M, Powell KS, Gatehouse JA, Raemaekers R, Newell CA, Hamilton WD. 1998. Production and purification of active snowdrop lectin in Escherichia coli. Eur J Biochem 252: 59–65.
  • Luo S, Zhangsun D, Tang K. 2005. Functional GNA expressed in Escherichia coli with high efficiency and its effect on Ceratovacuna lanigera Zehntner. Appl Microbiol Biotechnol 69: 184–191.
  • Ma Y, Shida H, Kawasaki T. 1997. Functional expression of human mannan-binding proteins (MBPs) in human hepatoma cell lines infected by recombinant vaccinia virus: post-translational modification, molecular assembly, and differentiation of serum and liver MBP. J Biochem 122: 810–818.
  • Maenuma K, Yim M, Komatsu K, Hoshino M, Takahashi Y, Bovin N, Irimura T. 2008. Use of a library of mutated Maackia amurensis hemagglutinin for profiling the cell lineage and differentiation. Proteomics 8: 3274–3283.
  • Markiv A, Peiris D, Curley GP, Odell M, Dwek MV. 2011. Identification, cloning, and characterization of two N-acetylgalactosamine-binding lectins from the albumen gland of Helix pomatia. J Biol Chem 286: 20260–20266.
  • Matsushita M, Ezekowitz RA, Fujita T. 1995. The Gly-54–>Asp allelic form of human mannose-binding protein (MBP) fails to bind MBP-associated serine protease. Biochem J 311 (Pt 3): 1021–1023.
  • Michelow IC, Dong M, Mungall BA, Yantosca LM, Lear C, Ji X, Karpel M, Rootes CL, Brudner M, Houen G, Eisen DP, Kinane TB, Takahashi K, Stahl GL, Olinger GG, Spear GT, Ezekowitz RA, Schmidt EV. 2010. A novel L-ficolin/mannose-binding lectin chimeric molecule with enhanced activity against Ebola virus. J Biol Chem 285: 24729–24739.
  • Michelow IC, Lear C, Scully C, Prugar LI, Longley CB, Yantosca LM, Ji X, Karpel M, Brudner M, Takahashi K, Spear GT, Ezekowitz RA, Schmidt EV, Olinger GG. 2011. High-dose mannose-binding lectin therapy for Ebola virus infection. J Infect Dis 203: 175–179.
  • Michiels K, Van Damme EJ, Smagghe G. 2010. Plant-insect interactions: what can we learn from plant lectins? Arch Insect Biochem Physiol 73: 193–212.
  • Mislovičová D, Gemeiner P, Kozarova A, Kožár T. 2009. Lectinomics I. Relevance of exogenous plant lectins in biomedical diagnostics. Biologia 64: 1–19.
  • Mori T, O’Keefe BR, Sowder RC 2nd, Bringans S, Gardella R, Berg S, Cochran P, Turpin JA, Buckheit RW Jr, McMahon JB, Boyd MR. 2005. Isolation and characterization of griffithsin, a novel HIV-inactivating protein, from the red alga Griffithsia sp. J Biol Chem 280: 9345–9353.
  • Nagahora H, Ishikawa K, Niwa Y, Muraki M, Jigami Y. 1992. Expression and secretion of wheat germ agglutinin by Saccharomyces cerevisiae. Eur J Biochem 210: 989–997.
  • Oguri S, Amano K, Nakashita H, Nagata Y, Momonoki YS. 2008. Molecular structure and properties of lectin from tomato fruit. Biosci Biotechnol Biochem 72: 2640–2650.
  • Ohba H, Bakalova R, Muraki M. 2003. Cytoagglutination and cytotoxicity of Wheat Germ Agglutinin isolectins against normal lymphocytes and cultured leukemic cell lines–relationship between structure and biological activity. Biochim Biophys Acta 1619: 144–150.
  • Ohtani K, Suzuki Y, Eda S, Kawai T, Kase T, Keshi H, Sakai Y, Yamamoto S, Sakamoto T, Wakamiya N. 1999. High-level and effective production of human mannan-binding lectin (MBL) in Chinese hamster ovary (CHO) cells. J Immunol Methods 222: 135–144.
  • Okamuro JK, Jofuku KD, Goldberg RB. 1986. Soybean seed lectin gene and flanking nonseed protein genes are developmentally regulated in transformed tobacco plants. Proc Natl Acad Sci USA 83: 8240–8244.
  • Olausson J, Aström E, Jonsson BH, Tibell LA, Påhlsson P. 2011. Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin. Glycobiology 21: 34–44.
  • Oliveira C, Felix W, Moreira RA, Teixeira JA, Domingues L. 2008. Expression of frutalin, an alpha-D-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris. Protein Expr Purif 60: 188–193.
  • Oliveira C, Costa S, Teixeira JA, Domingues L. 2009a. cDNA cloning and functional expression of the alpha-D-galactose-binding lectin frutalin in Escherichia coli. Mol Biotechnol 43: 212–220.
  • Oliveira C, Felix W, Moreira RA, Teixeira JA, Domingues L. 2008. Expression of frutalin, an alpha-D-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris. Protein Expr Purif 60: 188–193.
  • Oliveira C, Nicolau A, Teixeira JA, Domingues L. 2011. Cytotoxic effects of native and recombinant frutalin, a plant galactose-binding lectin, on HeLa cervical cancer cells. J Biomed Biotechnol 2011: 568932. doi:10.1155/2011/568932.
  • Oliveira C, Teixeira JA, Schmitt F, Domingues L. 2009b. A comparative study of recombinant and native frutalin binding to human prostate tissues. BMC Biotechnol 9: 78.
  • Petersen KA, Matthiesen F, Agger T, Kongerslev L, Thiel S, Cornelissen K, Axelsen M. 2006. Phase I safety, tolerability, and pharmacokinetic study of recombinant human mannan-binding lectin. J Clin Immunol 26: 465–475.
  • Peumans WJ, Van Damme EJ. 1995. Lectins as plant defense proteins. Plant Physiol 109: 347–352.
  • Pham Trung N, Fitches E, Gatehouse JA. 2006. A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects. BMC Biotechnol 6: 18.
  • Plattner VE, Wagner M, Ratzinger G, Gabor F, Wirth M. 2008. Targeted drug delivery: binding and uptake of plant lectins using human 5637 bladder cancer cells. Eur J Pharm Biopharm 70: 572–576.
  • Propheter DC, Hsu KL, Mahal LK. 2011. Recombinant lectin microarrays for glycomic analysis. Methods Mol Biol 723: 67–77.
  • Propheter DC, Mahal LK. 2011. Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis. Mol Biosyst 7: 2114–2117.
  • Pusztai A, Bardocz S, Ewen SW. 2008. Uses of plant lectins in bioscience and biomedicine. Front Biosci 13: 1130–1140.
  • Raemaekers RJ, de Muro L, Gatehouse JA, Fordham-Skelton AP. 1999. Functional phytohemagglutinin (PHA) and Galanthus nivalis agglutinin (GNA) expressed in Pichia pastoris correct N-terminal processing and secretion of heterologous proteins expressed using the PHA-E signal peptide. Eur J Biochem 265: 394–403.
  • Rahaie Z, Kazemi SS. 2010. Lectin-based biosensors: As powerful tools in bioanalytical applications. Biotechnology 4: 428–443.
  • Reed DG, Nopo-Olazabal LH, Funk V, Woffenden BJ, Reidy MJ, Dolan MC, Cramer CL, Medina-Bolivar F. 2005. Expression of functional hexahistidine-tagged ricin B in tobacco. Plant Cell Rep 24: 15–24.
  • Rosenfeld R, Bangio H, Gerwig GJ, Rosenberg R, Aloni R, Cohen Y, Amor Y, Plaschkes I, Kamerling JP, Maya RB. 2007. A lectin array-based methodology for the analysis of protein glycosylation. J Biochem Biophys Methods 70: 415–426.
  • Rüdiger H, Gabius HJ. 2001. Plant lectins: occurrence, biochemistry, functions and applications. Glycoconj J 18: 589–613.
  • Sahasrabuddhe AA, Ahmed N, Krishnasastry MV. 2006. Stress-induced phosphorylation of caveolin-1 and p38, and down-regulation of EGFr and ERK by the dietary lectin jacalin in two human carcinoma cell lines. Cell Stress Chaperones 11: 135–147.
  • Sahasrabuddhe AA, Gaikwad SM, Krishnasastry MV, Khan MI. 2004. Studies on recombinant single chain Jacalin lectin reveal reduced affinity for saccharides despite normal folding like native Jacalin. Protein Sci 13: 3264–3273.
  • Sato T, Hori K. 2009. Cloning, expression, and characterization of a novel anti-HIV lectin from the cultured cyanobacterium, Oscillatoria agardhii. Fish Sci 75: 743–753.
  • Sharon N, Lis H. 2004. History of lectins: from hemagglutinins to biological recognition molecules. Glycobiology 14: 53R–62R.
  • Sinha S, Surolia A. 2005. Oligomerization endows enormous stability to soybean agglutinin: a comparison of the stability of monomer and tetramer of soybean agglutinin. Biophys J 88: 4243–4251.
  • Song KK, Li DF, Zhang MC, Yang HJ, Ruan LW, Xu X. 2010. Cloning and characterization of three novel WSSV recognizing lectins from shrimp Marsupenaeus japonicus. Fish Shellfish Immunol 28: 596–603.
  • Sørensen HP, Mortensen KK. 2005. Advanced genetic strategies for recombinant protein expression in Escherichia coli. J Biotechnol 115: 113–128.
  • Stancombe PR, Alexander FC, Ling R, Matheson MA, Shone CC, Chaddock JA. 2003. Isolation of the gene and large-scale expression and purification of recombinant Erythrina cristagalli lectin. Protein Expr Purif 30: 283–292.
  • Streicher H, Sharon N. 2003. Recombinant plant lectins and their mutants. Meth Enzymol 363: 47–77.
  • Sumisa F, Ichijo N, Yamaguchi H, Nakatsumi H, Ando A, Iijima N, Oguri S, Uehara K, Nagata Y. 2004. Molecular properties of mycelial aggregate-specific lectin of Pleurotus cornucopiae. J Biosci Bioeng 98: 257–262.
  • Super M, Gillies SD, Foley S, Sastry K, Schweinle JE, Silverman VJ, Ezekowitz RA. 1992. Distinct and overlapping functions of allelic forms of human mannose binding protein. Nat Genet 2: 50–55.
  • Tague BW, Chrispeels MJ. 1987. The plant vacuolar protein, phytohemagglutinin, is transported to the vacuole of transgenic yeast. J Cell Biol 105: 1971–1979.
  • Tateno H, Winter HC, Goldstein IJ. 2004. Cloning, expression in Escherichia coli and characterization of the recombinant Neu5Acalpha2,6Galbeta1,4GlcNAc-specific high-affinity lectin and its mutants from the mushroom Polyporus squamosus. Biochem J 382: 667–675.
  • Thiel S, Jensenius J, Jensen T. 2004. Recombinant human mannan-binding lectin. USA Patent: 0,229,12.
  • Thiel S, Jensenius J, Jensen T. 2005. Recombinant human mannan-binding lectin. USA Patent: 6,846,649.
  • Tielker D, Rosenau F, Bartels KM, Rosenbaum T, Jaeger KE. 2006. Lectin-based affinity tag for one-step protein purification. BioTechniques 41: 327–332.
  • Tremblay R, Feng M, Menassa R, Huner NP, Jevnikar AM, Ma S. 2011. High-yield expression of recombinant soybean agglutinin in plants using transient and stable systems. Transgenic Res 20: 345–356.
  • Upadhyay SK, Saurabh S, Rai P, Singh R, Chandrashekar K, Verma PC, Singh PK, Tuli R. 2010. SUMO fusion facilitates expression and purification of garlic leaf lectin but modifies some of its properties. J Biotechnol 146: 1–8.
  • Van Damme EJ, Lanno N, Peumans WJ. 2008. Plant lectins. Adv Bot Res 48: 107–209.
  • Varejão N, Almeida Mda S, De Cicco NN, Atella GC, Coelho LC, Correia MA, Foguel D. 2010. Heterologous expression and purification of a biologically active legume lectin from Cratylia mollis seeds (CRAMOLL 1). Biochim Biophys Acta 1804: 1917–1924.
  • Voelker TA, Herman EM, Chrispeels MJ. 1989. In vitro mutated phytohemagglutinin genes expressed in tobacco seeds: role of glycans in protein targeting and stability. Plant Cell 1: 95–104.
  • Vonschaewen A, Chrispeels MJ. 1993. Identification of vacuolar sorting information in phytohemagglutinin, an unprocessed vacuolar protein. J Exp Bot 44: 339–342.
  • Vorup-Jensen T, Sørensen ES, Jensen UB, Schwaeble W, Kawasaki T, Ma Y, Uemura K, Wakamiya N, Suzuki Y, Jensen TG, Takahashi K, Ezekowitz RA, Thiel S, Jensenius JC. 2001. Recombinant expression of human mannan-binding lectin. Int Immunopharmacol 1: 677–687.
  • Wei J, Xu D, Zhou J, Cui H, Yan Y, Ouyang Z, Gong J, Huang Y, Huang X, Qin Q. 2010. Molecular cloning, characterization and expression analysis of a C-type lectin (Ec-CTL) in orange-spotted grouper, Epinephelus coioides. Fish Shellfish Immunol 28: 178–186.
  • Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J. 2007. Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry. J Biochem 141: 389–399.
  • Yang J, Wang L, Zhang H, Qiu L, Wang H, Song L. 2011. C-type lectin in Chlamys farreri (CfLec-1) mediating immune recognition and opsonization. PLoS ONE 6: e17089.
  • Yang N, Li DF, Feng L, Xiang Y, Liu W, Sun H, Wang DC. 2009. Structural basis for the tumor cell apoptosis-inducing activity of an antitumor lectin from the edible mushroom Agrocybe aegerita. J Mol Biol 387: 694–705.
  • Yang N, Tong X, Xiang Y, Zhang Y, Liang Y, Sun H, Wang DC. 2005. Molecular character of the recombinant antitumor lectin from the edible mushroom Agrocybe aegerita. J Biochem 138: 145–150.
  • Yim M, Ono T, Irimura T. 2001. Mutated plant lectin library useful to identify different cells. Proc Natl Acad Sci USA 98: 2222–2225.
  • Yin J, Li G, Ren X, Herrler G. 2007. Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes. J Biotechnol 127: 335–347.
  • Zelensky AN, Gready JE. 2005. The C-type lectin-like domain superfamily. FEBS J 272: 6179–6217.
  • Zwierzina H, Bergmann L, Fiebig H, Aamdal S, Schöffski P, Witthohn K, Lentzen H. 2011. The preclinical and clinical activity of aviscumine: a potential anticancer drug. Eur J Cancer 47: 1450–1457.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.