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Review Article

Revisiting microbial keratinases: next generation proteases for sustainable biotechnology

, &
Pages 216-228 | Received 18 Nov 2011, Accepted 11 Apr 2012, Published online: 30 May 2012

References

  • Awasthi P, Kushwaha RKS. 2011. New method for determination of feather degradation in soil. J Mycol Plant Pathol 41 (1):88–90.
  • Bakhtiar S, Estiveira RJ, Hatti-Kaul R. 2005 Substrate specifcity of alkaline protease from alkaliphilic feather-degrading Nesterenkonia sp AL20. Enzyme Microb Technol 37 (5):534–540.
  • Balaji S, Kumar MS, Karthikeyan R, Kumar R, Kirubanandan S, Sridhar R, Sehgal PK. 2008. Purification and characterization of an extracellular keratinase from a hornmeal-degrading Bacillus subtilis MTCC (9102). World J Microbiol Biotechnol 24(11):2741–2745.
  • Beg QK, Saxena RK, Gupta R. 2002. Kinetic constants determination for an alkaline protease from Bacillus mojavensis using response surface methodology. Biotechnol Bioeng 78: 289–295.
  • Bernal C, Cairo J, Coello N. 2006a. Purification and characterization of a novel exocellular keratinase from Kocuria rosea. Enzyme Microbial Technol 38(1–2):49–54.
  • Bernal C, Diaz I, Coello N. 2006. Response surface methodology for the optimization of keratinase production in culture medium containing feathers produced by Kocuria rosea. Can J Microbiol 52: 445–450.
  • Bihari Z, Vidéki D, Mihalik E, Szvetnik A, Szabó Z, Balázs M, Kesseru P, Kiss I. 2010. Degradation of native feathers by a novel keratinase-producing, thermophilic isolate, Brevibacillus thermoruber T1E. Z Naturforsch, C, J Biosci 65: 134–140.
  • BioResource-International 2011. Recent research results at poultry science association meeting. online available: http://www.briworldwide.com/bri-presents-recent-research-results-at-poultry-science-association-meeting/
  • Böckle B, Galunsky B, Müller R. 1995. Characterization of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530. Appl Environ Microbiol 61: 3705–3710.
  • Brandelli A. 2005. Hydrolysis of native proteins by a keratinolytic protease of Chryseobacterium sp. Ann Microbiol 55(1):47–50.
  • Brandelli A. 2008. Bacterial keratinases: useful enzymes for bioprocessing agroindustrial wastes and beyond Food Bioprocess Technol 1(2):105–116.
  • Brandelli A, Daroit DJ, Riffel A. 2010. Biochemical features of microbial keratinases and their production and applications. Appl Microbiol Biotechnol 85: 1735–1750.
  • Brandelli A, Riffel A. 2005 Production of an extracellular keratinase from Chryseobacterium sp growing on raw feathers. Electronic J Biotechnol 8 (1):35–42.
  • Burtt EH, Ichida JM. 1999 Keratinase produced by Bacillus licheniformis. 5877000.
  • Cai CG, Chen JS, Qi JJ, Yin Y, Zheng XD. 2008a. Purification and characterization of keratinase from a new Bacillus subtilis strain. J Zhejiang Univ Sci B 9: 713–720.
  • Cai CG, Lou BG, Zheng XD. 2008b. Keratinase production and keratin degradation by a mutant strain of Bacillus subtilis. J Zhejiang Univ Sci B 9: 60–67.
  • Cai SB, Huang ZH, Zhang XQ, Cao ZJ, Zhou MH, Hong F. 2011. Identification of a keratinase-producing bacterial strain and enzymatic study for its improvement on shrink resistance and tensile strength of wool- and polyester-blended fabric. Appl Biochem Biotechnol 163: 112–126.
  • Cao ZJ, Zhang Q, Wei DK, Chen L, Wang J, Zhang XQ, Zhou MH. 2009. Characterization of a novel Stenotrophomonas isolate with high keratinase activity and purification of the enzyme. J Ind Microbiol Biotechnol 36: 181–188.
  • Chao YP, Xie FH, Yang J, Lu JH, Qian SJ. 2007. Screening for a new Streptomyces strain capable of efficient keratin degradation. J Environ Sci (China) 19: 1125–1128.
  • Cherry JR, Fidantsef AL. 2003. Directed evolution of industrial enzymes: an update. Curr Opin Biotechnol 14: 438–443.
  • Daroit DJ, Correa APF, Brandelli A. 2011. Production of keratinolytic proteases through bioconversion of feather meal by the Amazonian bacterium Bacillus sp P45. Int Biodeterior Biodegradation 65(1):45–51.
  • Desai SS, Hegde S, Inamdar P, Sake N, Aravind MS. 2010. Isolation of keratinase from bacterial isolates of poultry soil for waste degradation. Eng Life Sci 10(4):361–367.
  • Esawy MA. 2007. Isolation and partial characterization of extracellular keratinase from a novel mesophilic Streptomyces albus AZA. Res J Agric Biol Sci 3 (6):10.
  • Evans KL, Crowder J, Miller ES. 2000. Subtilisins of Bacillus spp. hydrolyze keratin and allow growth on feathers. Can J Microbiol 46: 1004–1011.
  • Fakhfakh-Zouari N, Hmidet N, Haddar A, Kanoun S, Nasri M. 2010. A novel serine metallokeratinase from a newly isolated Bacillus pumilus A1 grown on chicken feather meal: biochemical and molecular characterization. Appl Biochem Biotechnol 162: 329–344.
  • Ferrer M, Martínez-Abarca F, Golyshin PN. 2005. Mining genomes and ‘metagenomes’ for novel catalysts. Curr Opin Biotechnol 16: 588–593.
  • Freeman SR, Poore MH, Middleton TF, Ferket PR. 2009. Alternative methods for disposal of spent laying hens: evaluation of the efficacy of grinding, mechanical deboning, and of keratinase in the rendering process. Bioresour Technol 100: 4515–4520.
  • Friedrich J, Gradisar H, Vrecl M, Pogacnik A. 2005 In vitro degradation of porcine skin epidermis by a fungal keratinase of Doratomyces microsporus. Enzyme Microbial Technol 36(4):455–460.
  • Gradisar H, Friedrich J, Krizaj I, Jerala R. 2005. Similarities and specificities of fungal keratinolytic proteases: comparison of keratinases of Paecilomyces marquandii and Doratomyces microsporus to some known proteases. Appl Environ Microbiol 71: 3420–3426.
  • Gradisar H, Kern S, Friedrich J. 2000. Keratinase of Doratomyces microsporus. Appl Microbiol Biotechnol 53: 196–200.
  • Guazzaroni ME, Beloqui A, Golyshin PN, Ferrer M. 2009. Metagenomics as a new technological tool to gain scientific knowledge. World J Microbiol Biotechnol 25(6):945–954.
  • Gupta R, Ramnani P. 2006. Microbial keratinases and their prospective applications: an overview. Appl Microbiol Biotechnol 70: 21–33.
  • Hui Z, Doi H, Kanouchi H, Matsuura Y, Mohri S, Nonomura Y, Oka T. 2004. Alkaline serine protease produced by Streptomyces sp. degrades PrP(Sc). Biochem Biophys Res Commun 321: 45–50.
  • Ionata E, Canganella F, Bianconi G, Benno Y, Sakamoto M, Capasso A, Rossi M, La Cara F. 2008. A novel keratinase from Clostridium sporogenes bv. pennavorans bv. nov., a thermotolerant organism isolated from solfataric muds. Microbiol Res 163: 105–112.
  • Jayalakshmi T, Krishnamoorthy P, Rameshkumar G, Sivamani P. 2011. Docking conformation of Bacillus licheniformis keratinase enzyme and PMSF ligand. Drug Invention Today 3(8):3.
  • Jeong JH, Park KH, Oh DJ, Hwang DY, Kim HS, Lee CY, Son HJ. 2010. Keratinolytic enzyme-mediated biodegradation of recalcitrant feather by a newly isolated Xanthomonas sp. P5. Polymer Degrad Stabil 95(10):1969–1977.
  • Kaur J, Sharma R. 2006. Directed evolution: an approach to engineer enzymes. Crit Rev Biotechnol 26: 165–199.
  • Kim JS, Kluskens LD, de Vos WM, Huber R, van der Oost J. 2004. Crystal structure of fervidolysin from Fervidobacterium pennivorans, a keratinolytic enzyme related to subtilisin. J Mol Biol 335: 787–797.
  • Kluskens LD, Voorhorst WG, Siezen RJ, Schwerdtfeger RM, Antranikian G, van der Oost J, de Vos WM. 2002. Molecular characterization of fervidolysin, a subtilisin-like serine protease from the thermophilic bacterium Fervidobacterium pennivorans. Extremophiles 6: 185–194.
  • Kublanov IV, Tsirul’nikov KB, Kaliberda EN, Rumsh LD, Haertle T, Bonch-Osmolovskaia EA. 2009. Keratinase of an anaerobic thermophilic bacterium Thermoanaerobacter sp. strain 1004-09 isolated from a hot spring in the Baikal Rift zone. Mikrobiologiia 78: 79–88.
  • Lakshmipathy DT, Kannabiran K. 2010. Review on dermatomycosis: pathogenesis and treatment. Nat Sci 2(7):726–731.
  • Langeveld JP, Wang JJ, Van de Wiel DF, Shih GC, Garssen GJ, Bossers A, Shih JC. 2003. Enzymatic degradation of prion protein in brain stem from infected cattle and sheep. J Infect Dis 188: 1782–1789.
  • Lin HH, Yin LJ, Jiang ST. 2009. Cloning, expression, and purification of Pseudomonas aeruginosa keratinase in Escherichia coli AD494(DE3)pLysS expression system. J Agric Food Chem 57: 3506–3511.
  • Lin HH, Yin LJ, Jiang ST. 2009. Expression and purification of Pseudomonas aeruginosa keratinase in Bacillus subtilis DB104 expression system. J Agric Food Chem 57: 7779–7784.
  • Lin HH, Yin LJ, Jiang ST. 2009. Functional expression and characterization of keratinase from Pseudomonas aeruginosa in Pichia pastoris. J Agric Food Chem 57: 5321–5325.
  • Lin X, Kelemen DW, Miller ES, Shih JC. 1995. Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1. Appl Environ Microbiol 61: 1469–1474.
  • Lin X, Wong SL, Miller ES, Shih JC. 1997. Expression of the Bacillus licheniformis PWD-1 keratinase gene in B. subtilis. J Ind Microbiol Biotechnol 19: 134–138.
  • Macedo AJ, Beys da Silva WO, Termignoni C. 2008. Properties of a non collagen-degrading Bacillus subtilis keratinase. Can J Microbiol 54: 180–188.
  • Malhotra GG, Zatz JL. 2002. Investigation of nail permeation enhancement by chemical modification using water as a probe. J Pharm Sci 91: 312–323.
  • Maurer KH. 2004. Detergent proteases. Curr Opin Biotechnol 15: 330–334.
  • Mazotto AM, Cedrola SM, Lins U, Rosado AS, Silva KT, Chaves JQ, Rabinovitch L, Zingali RB, Vermelho AB. 2010. Keratinolytic activity of Bacillus subtilis AMR using human hair. Lett Appl Microbiol 50: 89–96.
  • Mazotto AM, de Melo ACN, Macrae A, Rosado AS, Peixoto R, Cedrola SML, Couri S, Zingali RB, Villa ALV, Rabinovitch L, Chaves JQ, Vermelho AB. 2011. Biodegradation of feather waste by extracellular keratinases and gelatinases from Bacillus spp. World J Microbiol Biotechnol 27(6):1355–1365.
  • McLeod AH, Murdoch H, Dickinson J, Dennis MJ, Hall GA, Buswell CM, Carr J, Taylor DM, Sutton JM, Raven ND. 2004. Proteolytic inactivation of the bovine spongiform encephalopathy agent. Biochem Biophys Res Commun 317: 1165–1170.
  • Mitsuiki S, Hui Z, Matsumoto D, Sakai M, Moriyama Y, Furukawa K, Kanouchi H, Oka T. 2006. Degradation of PrP(Sc) by keratinolytic protease from Nocardiopsis sp. TOA-1. Biosci Biotechnol Biochem 70: 1246–1248.
  • Mitsuiki S, Ichikawa M, Oka T, Sakai M, Moriyama Y, Sameshima Y, Goto M, Furukawa K. 2004. Molecular characterization of a keratinolytic enzyme from an alkaliphilic Nocardiopsis sp TOA-1. Enzyme Microb Technol 34(5):482–489.
  • Mohorcic M, Torkar A, Friedrich J, Kristl J, Murdan S. 2007. An investigation into keratinolytic enzymes to enhance ungual drug delivery. Int J Pharm 332: 196–201.
  • Mosesson MW. 2005. Fibrinogen and fibrin structure and functions. J Thromb Haemost 3: 1894–1904.
  • Murdan S. 2002. Drug delivery to the nail following topical application. Int J Pharm 236: 1–26.
  • Onifade AA, Al-Sane NA, Al-Musallam AA, Al-Zarban S. 1998. A review: Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresource Technol 66(1):1–11.
  • Papadopoulos MC. 1986. The effect of enzymatic treatment on amino-acid content and nitrogen characteristics of feather meal. Anim Feed Sci Technol 16(1–2):151–156.
  • Park GT, Son HJ. 2009. Keratinolytic activity of Bacillus megaterium F7-1, a feather-degrading mesophilic bacterium. Microbiol Res 164: 478–485.
  • Prakash P, Jayalakshmi SK, Sreeramulu K. 2010a. Production of keratinase by free and immobilized cells of Bacillus halodurans strain PPKS-2: partial characterization and its application in feather degradation and dehairing of the goat skin. Appl Biochem Biotechnol 160: 1909–1920.
  • Prakash P, Jayalakshmi SK, Sreeramulu K. 2010b. Purification and characterization of extreme alkaline, thermostable keratinase, and keratin disulfide reductase produced by Bacillus halodurans PPKS-2. Appl Microbiol Biotechnol 87: 625–633.
  • Radha S, Gunasekaran P. 2009. Purification and characterization of keratinase from recombinant Pichia and Bacillus strains. Protein Expr Purif 64: 24–31.
  • Rajput R, Sharma R, Gupta R. 2010. Biochemical characterization of a thiol-activated, oxidation stable keratinase from Bacillus pumilus KS12. Enzyme Res 2010: 132148.
  • Ramnani P, Gupta R. 2007. Keratinases vis-a-vis conventional proteases and feather degradation. World J Microbiol Biotechnol 23(11):1537–1540.
  • Ramnani P, Singh R, Gupta R. 2005. Keratinolytic potential of Bacillus licheniformis RG1: structural and biochemical mechanism of feather degradation. Can J Microbiol 51: 191–196.
  • Rozs M, Manczinger L, Vágvölgyi C, Kevei F, Hochkoeppler A, Vara y Rodríguez AG. 2001. Fermentation characteristics and secretion of proteases of a new keratinolytic strain of Bacillus licheniformis. Biotechnol Lett 23(23):1925–1929.
  • Sangali S, Brandelli A. 2000a. Feather keratin hydrolysis by a Vibrio sp. strain kr2. J Appl Microbiol 89: 735–743.
  • Sangali S, Brandelli A. 2000b. Isolation and characterization of a novel feather-degrading bacterial strain. Appl Biochem Biotechnol 87: 17–24.
  • Sharma R, Gupta R. 2010a. Extracellular expression of keratinase Ker P from Pseudomonas aeruginosa in E. coli. Biotechnol Lett 32: 1863–1868.
  • Sharma R, Gupta R. 2010b. Substrate specificity characterization of a thermostable keratinase from Pseudomonas aeruginosa KS-1. J Ind Microbiol Biotechnol 37: 785–792.
  • Sharma R, Murty NA, Gupta R. 2011. Molecular characterization of N-terminal pro-sequence of keratinase Ker P from Pseudomonas aeruginosa: identification of region with chaperone activity. Appl Biochem Biotechnol 165: 892–901.
  • Shih JCH, Williams CM 1992. Purified Bacillus licheniformis PWD-1 keratinase. US Patent 5171682.
  • Silveira ST, Casarin F, Gemelli S, Brandelli A. 2010. Thermodynamics and kinetics of heat inactivation of a novel keratinase from Chryseobacterium sp. strain kr6. Appl Biochem Biotechnol 162: 548–560.
  • Silveira ST, Jaeger MK, Brandelli A. 2009. Kinetic data and substrate specificity of a keratinase from Chryseobacterium sp strain kr6. J Chem Technol Biotechnol 84(3), 361–366.
  • Sousa F, Jus S, Erbel A, Kokol V, Cavaco-Paulo A, Gubitz GM. 2007. A novel metalloprotease from Bacillus cereus for protein fibre processing. Enzyme Microb Technol 40:1772–1781.
  • Steele HL, Jaeger KE, Daniel R, Streit WR. 2009. Advances in recovery of novel biocatalysts from metagenomes. J Mol Microbiol Biotechnol 16: 25–37.
  • Sun Y, Liu JC, Kimbelton ES, Wang JCT. 1997. Antifungal treatment of nails. US patent 5696164.
  • Suzuki Y, Tsujimoto Y, Matsui H, Watanabe K. 2006. Decomposition of extremely hard-to-degrade animal proteins by thermophilic bacteria. J Biosci Bioeng 102: 73–81.
  • Syed DG, Lee JC, Li WJ, Kim CJ, Agasar D. 2009. Production, characterization and application of keratinase from Streptomyces gulbargensis. Bioresour Technol 100: 1868–1871.
  • Taylor DM. 2000. Inactivation of transmissible degenerative encephalopathy agents: A review. Vet J 159: 10–17.
  • Teresa K-K, Justyna B. 2011. Biodegradation of keratin waste: Theory and practical aspects. Waste Manag 31(8):1689–1701.
  • Tiwary E, Gupta R. 2010. Improved catalytic efficiency of a monomeric gamma-glutamyl transpeptidase from Bacillus licheniformis in presence of subtilisin. Biotechnol Lett 32: 1137–1141.
  • Tsiroulnikov K, Rezai H, Bonch-Osmolovskaya E, Nedkov P, Gousterova A, Cueff V, Godfroy A, Barbier G, Métro F, Chobert JM, Clayette P, Dormont D, Grosclaude J, Haertlé T. 2004. Hydrolysis of the amyloid prion protein and nonpathogenic meat and bone meal by anaerobic thermophilic prokaryotes and Streptomyces subspecies. J Agric Food Chem 52: 6353–6360.
  • Valetti F, Gilardi G. 2004. Directed evolution of enzymes for product chemistry. Nat Prod Rep 21: 490–511.
  • Vignardet C, Guillaume YC, Michel L, Friedrich J, Millet J. 2001. Comparison of two hard keratinous substrates submitted to the action of a keratinase using an experimental design. Int J Pharm 224: 115–122.
  • Voet D, Voet JG. 1995 Three-dimensional structure of proteins, 2nd ed. New York: Wiley.
  • Wang JJ, Greenhut WB, Shih JC. 2005. Development of an asporogenic Bacillus licheniformis for the production of keratinase. J Appl Microbiol 98: 761–767.
  • Wang JJ, Rojanatavorn K, Shih JC. 2004. Increased production of Bacillus keratinase by chromosomal integration of multiple copies of the kerA gene. Biotechnol Bioeng 87: 459–464.
  • Wang JJ, Swaisgood HE, Shih JC. 2003. Bioimmobilization of keratinase using Bacillus subtilis and Escherichia coli systems. Biotechnol Bioeng 81: 421–429.
  • Wang P, Wang Q, Cui L, Gao M, Fan X. 2011 The combined use of cutinase, keratinase and protease treatments for wool bio-antifelting. Fibers and Polymers 12(6):760–764.
  • Wang SL, Hsu WT, Liang TW, Yen YH, Wang CL. 2008. Purification and characterization of three novel keratinolytic metalloproteases produced by Chryseobacterium indologenes TKU014 in a shrimp shell powder medium. Bioresour Technol 99: 5679–5686.
  • Williams CM, Richter CS, Mackenzie JM, Shih JC. 1990. Isolation, identification, and characterization of a feather-degrading bacterium. Appl Environ Microbiol 56: 1509–1515.
  • Xie F, Chao Y, Yang X, Yang J, Xue Z, Luo Y, Qian S. 2010. Purification and characterization of four keratinases produced by Streptomyces sp. strain 16 in native human foot skin medium. Bioresour Technol 101: 344–350.
  • Yamamura S, Morita Y, Hasan Q, Yokoyama K, Tamiya E. 2002. Keratin degradation: a cooperative action of two enzymes from Stenotrophomonas sp. Biochem Biophys Res Commun 294: 1138–1143.
  • Yoshioka M, Miwa T, Horii H, Takata M, Yokoyama T, Nishizawa K, Watanabe M, Shinagawa M, Murayama Y. 2007. Characterization of a proteolytic enzyme derived from a Bacillus strain that effectively degrades prion protein. J Appl Microbiol 102: 509–515.
  • Yue XY, Zhang B, Jiang DD, Liu YJ, Niu TG. 2011 Separation and purification of a keratinase as pesticide against root-knot nematodes. World J Microbiol Biotechnol, 27 (9): 2147–2153.
  • Yuichi M, Miako Y, Matsuhiro M, Takehiro M, Satoru K, Koji N. 2003 A novel proteolytic enzyme that digests PrPSc. Topics Anim Health Res 2:2.
  • Zaghloul TI. 1998. Cloned Bacillus subtilis alkaline protease (aprA) gene showing high level of keratinolytic activity. Appl Biochem Biotechnol 70-72: 199–205.
  • Zaghloul TI, Embaby AM, Elmahdy AR. 2011. Key determinants affecting sheep wool biodegradation directed by a keratinase-producing Bacillus subtilis recombinant strain. Biodegradation 22: 111–128.

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