Flow Cytometry and Solid Organ Transplantation: A Perfect Match

References

• Ahmed M, Venkataraman R, Logar AJ, et al. (2001). Quantitation of immunosuppression by tacrolimus using flow cytometric analysis of interleukin-2 and interferon-gamma inhibition in CD8(-) and CD8(+) peripheral blood T cells. Thera Drug Monitor, 23, 354–62
   PubMed Web of Science ®Google Scholar
• Akalin E, Pascual M. (2006). Sensitization after kidney transplantation. Clin J Am Soc Nephrol, 1, 433–40
   PubMed Web of Science ®Google Scholar
• Akulian JA, Pipeling MR, John ER, et al. (2013). High-quality CMV-specific CD4+ memory is enriched in the lung allograft and is associated with mucosal viral control. Amer J Transplant, 13, 146–56
   PubMed Web of Science ®Google Scholar
• Allen U, Preiksaitis J. (2009). Epstein-Barr virus and posttransplant lymphoproliferative disorder in solid organ transplant recipients. Amer J Transplant, 9, S87–96
   PubMed Web of Science ®Google Scholar
• Altman JD, Moss PA, Goulder PJ, et al. (1996). Phenotypic analysis of antigen-specific T lymphocytes. Science, 274, 94–6
   PubMed Web of Science ®Google Scholar
• Augustine JJ, Hricik DE. (2012). T-cell immune monitoring by the ELISPOT assay for interferon gamma. Clin Chim Acta, 413, 1359–63
   PubMed Web of Science ®Google Scholar
• Autissier P, Soulas C, Burdo TH, Williams KC. (2010). Evaluation of a 12-color flow cytometry panel to study lymphocyte, monocyte, and dendritic cell subsets in humans. Cytometry Pt A, 77, 410–9
   PubMed Web of Science ®Google Scholar
• Baan C, Bouvy A, Vafadari R, Weimar W. (2012). Phospho-specific flow cytometry for pharmacodynamic monitoring of immunosuppressive therapy in transplantation. Transplant Res, 1, 20
   PubMedGoogle Scholar
• Bate SL, Dollard SC, Cannon MJ. (2010). Cytomegalovirus seroprevalence in the United States: The national health and nutrition examination surveys, 1988–2004. Clin Infect Dis, 50, 1439–47
   PubMed Web of Science ®Google Scholar
• Battaglia M, Stabilini A, Roncarolo MG. (2005). Rapamycin selectively expands CD4+CD25+FoxP3+ regulatory T cells. Blood, 105, 4743–8
   PubMed Web of Science ®Google Scholar
• Bendall SC, Simonds EF, Qiu P, et al. (2011). Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science, 332, 687–96
   PubMed Web of Science ®Google Scholar
• Bercovici N, Givan AL, Waugh MG, et al. (2003). Multiparameter precursor analysis of T-cell responses to antigen. J Immunol Meth, 276, 5–17
   PubMed Web of Science ®Google Scholar
• Blanco-Garcia RM, Lopez-Alvarez MR, Garrido IP, et al. (2011). CD28 and KIR2D receptors as sensors of the immune status in heart and liver transplantation. Human Immunol, 72, 841–8
   PubMed Web of Science ®Google Scholar
• Boenisch O, Lopez M, Elyaman W, et al. (2012). Ex vivo expansion of human Tregs by rabbit ATG is dependent on intact STAT3-signaling in CD4(+) T cells and requires the presence of monocytes. Amer J Transplant, 12, 856–66
   PubMed Web of Science ®Google Scholar
• Boleslawski E, Othman SB, Aoudjehane L, et al. (2011). CD28 expression by peripheral blood lymphocytes as a potential predictor of the development of de novo malignancies in long-term survivors after liver transplantation. Liver Transplant, 17, 299–305
   PubMed Web of Science ®Google Scholar
• Brooimans RA, Boyce CS, Popma J, et al. (2008). Analytical performance of a standardized single-platform MHC tetramer assay for the identification and enumeration of CMV-specific CD8+ T lymphocytes. Cytom Pt A, 73, 992–1000
   PubMed Web of Science ®Google Scholar
• Bunde T, Kirchner A, Hoffmeister B, et al. (2005). Protection from cytomegalovirus after transplantation is correlated with immediate early 1-specific CD8 T cells. J Exper Med, 201, 1031–6
   PubMed Web of Science ®Google Scholar
• Cantisan S, Torre-Cisneros J, Lara R, et al. (2013). Impact of cytomegalovirus on early immunosenescence of CD8+ T lymphocytes after solid organ transplantation. J Gerontol Ser A, 68, 1–5
   PubMed Web of Science ®Google Scholar
• Colovai Al, Mirza M, Vlad G, et al. (2003). Regulatory CD8+CD28- T cells in heart transplant recipients. Human Immunol, 64, 31–7
   PubMed Web of Science ®Google Scholar
• Colvin R. (2007). Antibody-mediated rejection in renal allografts: Diagnosis and pathogenesis. J Am Soc Nephrol, 18, 1046–56
   PubMed Web of Science ®Google Scholar
• Cosimi AB, Colvin RB, Burton RC, et al. (1981). Use of monoclonal antibodies to T-cell subsets for immunologic monitoring and treatment in recipients of renal allografts. New Engl J Med, 305, 308–14
   PubMed Web of Science ®Google Scholar
• Coutinho AE, Chapman KE. (2011). The anti-inflammatory and immunosuppressive effects of glucocorticoids, recent developments and mechanistic insights. Mol Cell Endocrinol, 335(1), 2–13
   PubMed Web of Science ®Google Scholar
• Cummins NW, Deziel PJ, Abraham RS, Razonable RR. (2009). Deficiency of cytomegalovirus (CMV)-specific CD8+ T cells in patients presenting with late-onset CMV disease several years after transplantation. Transpl Infect Dis, 11, 20–7
   PubMed Web of Science ®Google Scholar
• Daniel V, Naujokat C, Sadeghi M, et al. (2008). Observational support for an immunoregulatory role of CD3+CD4+CD25+IFN-gamma+ blood lymphocytes in kidney transplant recipients with good long-term graft outcome. Transpl Inter, 21, 646–60
   PubMed Web of Science ®Google Scholar
• Daniel V, Sadeghi M, Wang H, Opelz G. (2012). In-vitro inhibition of IFNgamma+ iTreg mediated by monoclonal antibodies against cell surface determinants essential for iTreg function. BMC Immunol, 13, 47
   PubMed Web of Science ®Google Scholar
• De Rosa SC, Herzenberg LA, Roederer M. (2001). 11-color, 13-parameter flow cytometry: Identification of human naive T cells by phenotype, function, and T-cell receptor diversity. Nat Med, 7, 245–8
   PubMed Web of Science ®Google Scholar
• De Serres SA, Mfarrej BG, Grafals M, et al. (2012). Derivation and validation of a cytokine-based assay to screen for acute rejection in renal transplant recipients. Clin J Amer Soc Nephrology: CJASN, 7, 1018–25
   PubMed Web of Science ®Google Scholar
• Dieterlen MT, Bittner HB, Klein S, et al. (2012). Assay validation of phosphorylated S6 ribosomal protein for a pharmacodynamic monitoring of mTOR-inhibitors in peripheral human blood. Cytomet Pt B, 82, 151–7
   PubMed Web of Science ®Google Scholar
• Dumont FJ, Su Q. (1996). Mechanism of action of the immunosuppressant rapamycin. Life Sci, 58, 373–95
   PubMed Web of Science ®Google Scholar
• Egli A, Silva M Jr., O'Shea D, et al. (2012). An analysis of regulatory T-cell and Th-17 cell dynamics during cytomegalovirus replication in solid organ transplant recipients. PloS One, 7, e43937
   PubMed Web of Science ®Google Scholar
• Eid AJ, Brown RA, Arthurs SK, et al. (2010). A prospective longitudinal analysis of cytomegalovirus (CMV)-specific CD4+ and CD8+ T cells in kidney allograft recipients at risk of CMV infection. Transpl Inter, 23, 506–13
   PubMed Web of Science ®Google Scholar
• Erie AJ, Samsel L, Takaku T, et al. (2011). MHC class II upregulation and colocalization with Fas in experimental models of immune-mediated bone marrow failure. Exper Hematol, 39, 837–49
   PubMed Web of Science ®Google Scholar
• Everly M, Everly JJ, Arend LJ, et al. (2009). Reducing de novo donor-specific antibody levels during acute rejection diminishes renal allograft loss. Amer J Transplant, 9, 1063–71
   PubMed Web of Science ®Google Scholar
• Ferguson R, Grinyo J, Vincenti F, et al. (2011). Immunosuppression with belatacept-based, corticosteroid-avoiding regimens in de novo kidney transplant recipients. Amer J Transpl, 11, 66–76
   PubMed Web of Science ®Google Scholar
• Frischbutter S, Schultheis K, Patzel M, et al. (2012). Evaluation of calcineurin/NFAT inhibitor selectivity in primary human Th cells using bar-coding and phospho-flow cytometry. Cytom Pt A, 81, 1005–11
   PubMed Web of Science ®Google Scholar
• Fuhrmann S, Lachmann R, Streitz M. (2012). Cyclosporin A and tacrolimus reduce T-cell polyfunctionality but not interferon-gamma responses directed at cytomegalovirus. Immunology, 136, 408–13
   PubMed Web of Science ®Google Scholar
• Geissler EK. (2012). The ONE Study compares cell therapy products in organ transplantation: Introduction to a review series on suppressive monocyte-derived cells. Transplant Res, 1, 11
   PubMedGoogle Scholar
• Gerna G, Lilleri D, Chiesa A, et al. (2011). Virologic and immunologic monitoring of cytomegalovirus to guide preemptive therapy in solid-organ transplantation. Amer J Transplant, 11, 2463–71
   PubMed Web of Science ®Google Scholar
• Gokmen R, Hernandez-Fuentes MP. (2013). Biomarkers of tolerance. Curr Opin Organ Transplant, 18, 416–20
   PubMed Web of Science ®Google Scholar
• Guppy AE, Rawlings E, Madrigal JA, et al. (2007). A quantitative assay for Epstein-Barr Virus-specific immunity shows interferon-gamma producing CD8+ T cells increase during immunosuppression reduction to treat posttransplant lymphoproliferative disease. Transplantation, 84, 1534–9
   PubMed Web of Science ®Google Scholar
• Han Q, Bagheri N, Bradshaw EM, et al. (2012). Polyfunctional responses by human T cells result from sequential release of cytokines. Proceedings of the National Academy of Sciences of the United States of America, 109, 1607–12
   PubMed Web of Science ®Google Scholar
• Hester J, Schiopu A, Nadig SN, Wood, K.J. (2012). Low-dose rapamycin treatment increases the ability of human regulatory T cells to inhibit transplant arteriosclerosis in vivo. Amer J Transplant, 12, 2008–16
   PubMed Web of Science ®Google Scholar
• Hippen KL, Merkel SC, Schirm DK, et al. (2011). Massive ex vivo expansion of human natural regulatory T cells (T(regs)) with minimal loss of in vivo functional activity. Sci Trans Med, 3, 83ra41
   PubMed Web of Science ®Google Scholar
• Hiririan A, Kiangkitiwan B, Kukaruga D, et al. (2009). The impact of C4d pattern and donor-specific antibody on graft survival in recipients requiring indication renal allograft biopsy. Amer J Transplant, 9, 2758–67
   PubMed Web of Science ®Google Scholar
• Hock BD, Mackenzie KA, Cross NB, et al. (2012). Renal transplant recipients have elevated frequencies of circulating myeloid-derived suppressor cells. Nephrol Dial Transplant, 27, 402–10
   PubMedGoogle Scholar
• Hope CM, Grace BS, Pilkington KR, et al. (2014). The immune phenotype may relate to cancer development in kidney transplant recipients. Kidney Inter, in press
   Google Scholar
• Humar A, Lebranchu Y, Vincenti F, et al. (2010). The efficacy and safety of 200 days valganciclovir cytomegalovirus prophylaxis in high-risk kidney transplant recipients. Amer J Transplant, 10, 1228–37
   PubMed Web of Science ®Google Scholar
• Humar A, Mazzulli T, Moussa G, et al. (2005). Clinical utility of cytomegalovirus (CMV) serology testing in high-risk CMV D+/R- transplant recipients. Amer J Transplant, 5, 1065–70
   PubMed Web of Science ®Google Scholar
• Humar A, Michaels M. (2006). American Society of Transplantation recommendations for screening, monitoring and reporting of infectious complications in immunosuppression trials in recipients of organ transplantation. Amer J Transplant, 6, 262–74
   PubMed Web of Science ®Google Scholar
• Hutchinson JA, Riquelme P, Sawitzki B, et al. (2011). Cutting Edge: Immunological consequences and trafficking of human regulatory macrophages administered to renal transplant recipients. J Immunol, 187, 2072–8
   Google Scholar
• Imadome K, Fukuda A, Kawano F, et al. (2012). Effective control of Epstein-Barr virus infection following pediatric liver transplantation by monitoring of viral DNA load and lymphocyte surface markers. Pediat Transplant, 16, 748–57
   PubMed Web of Science ®Google Scholar
• Jungraithmayr W, Codarri L, Bouchaud G, et al. (2013). Cytokine complex-expanded natural killer cells improve allogeneic lung transplant function via depletion of donor dendritic cells. Amer J Respir Crit Care Med, 187, 1349–59
   PubMed Web of Science ®Google Scholar
• Kotton CN. (2013). CMV: Prevention, diagnosis and therapy. Amer J Transplant, 13, 24–40; quiz 40
   PubMed Web of Science ®Google Scholar
• Kotton CN, Kumar D, Caliendo AM, et al. (2010). International consensus guidelines on the management of cytomegalovirus in solid organ transplantation. Transplantation, 89, 779–95
   PubMed Web of Science ®Google Scholar
• Krystufkova E, Sekerkova A, Striz I, et al. (2012). Regulatory T cells in kidney transplant recipients: The effect of induction immunosuppression therapy. Nephrol Dial, Transpl, 27, 2576–82
   PubMed Web of Science ®Google Scholar
• Kumar D, Chernenko S, Moussa G, et al. (2009). Cell-mediated immunity to predict cytomegalovirus disease in high-risk solid organ transplant recipients. Amer J Transplant, 9, 1214–22
   PubMed Web of Science ®Google Scholar
• La Rosa C, Limaye AP, Krishnan A, et al. (2007). Longitudinal assessment of cytomegalovirus (CMV)-specific immune responses in liver transplant recipients at high risk for late CMV disease. J Infect Dis, 195, 633–44
   PubMed Web of Science ®Google Scholar
• Larsen CP, Pearson TC, Adams AB, et al. (2005). Rational development of LEA29Y (belatacept), a high-affinity variant of CTLA4-Ig with potent immunosuppressive properties. Amer J Transpl, 5, 443–53
   PubMed Web of Science ®Google Scholar
• Lees JR, Azimzadeh AM, Bromberg JS. (2011). Myeloid derived suppressor cells in transplantation. Curr Opin Immunol, 23, 692–7
   PubMed Web of Science ®Google Scholar
• Lefaucheur C, Nochy D, Andrade J, et al. (2009). Comparison of Combination Plasmapheresis/IVIg/Anti-CD20 Versus High-Dose IVIg in the Treatment of Antibody-Mediated Rejection. Amer J Transplant, 9, 1099–107
   PubMed Web of Science ®Google Scholar
• Leigh ND, Bian G, Ding X, et al. (2014). A flagellin-derived toll-like receptor 5 agonist stimulates cytotoxic lymphocyte-mediated tumor immunity. PloS One, 9, e85587
   PubMed Web of Science ®Google Scholar
• Li Y, Koshiba T, Yoshizawa A, et al. (2004). Analyses of peripheral blood mononuclear cells in operational tolerance after pediatric living donor liver transplantation. American journal of transplantation: Official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 4, 2118–25
   PubMed Web of Science ®Google Scholar
• Loewendorf A, Csete M. (2013). Concise review: Immunologic lessons from solid organ transplantation for stem cell-based therapies. Stem Cells Trans Med, 2, 136–42
   PubMed Web of Science ®Google Scholar
• Macedo C, Webber SA, Donnenberg AD, et al. (2011). EBV-specific CD8+ T cells from asymptomatic pediatric thoracic transplant patients carrying chronic high EBV loads display contrasting features: Activated phenotype and exhausted function. J Immunol, 186, 5854–62
   PubMed Web of Science ®Google Scholar
• Maguire O, Chen G, O’Loughlin K, et al. (2013a). Simultaneous assessment of CMV specificity and functional response CD8+ T cells from bone marrow transplant recipients. Proceedings of the XXVIII Congress of the International Society for Advancement of Cytometry, Abstract # 145
   Google Scholar
• Maguire O, Collins C, O'Loughlin K, et al. (2011). Quantifying nuclear p65 as a parameter for NF-kappaB activation: Correlation between ImageStream cytometry, microscopy, and Western blot. Cytom Pt A, 79, 461–9
   PubMed Web of Science ®Google Scholar
• Maguire O, Tornatore KM, O'Loughlin KL, et al. (2013b). Nuclear translocation of nuclear factor of activated T cells (NFAT) as a quantitative pharmacodynamic parameter for tacrolimus. Cytom Pt A, 83, 1096–104
   PubMed Web of Science ®Google Scholar
• Martinez-Llordella M, Puig-Pey I, Orlando G, et al. (2007). Multiparameter immune profiling of operational tolerance in liver transplantation. Amer J Transplant, 7, 309–19
   PubMed Web of Science ®Google Scholar
• Mazariegos GV, Zahorchak AF, Reyes J, et al. (2003). Dendritic cell subset ratio in peripheral blood correlates with successful withdrawal of immunosuppression in liver transplant patients. Amer J Transplant, 3, 689–96
   PubMed Web of Science ®Google Scholar
• Najafian N, Albin MJ, Newell KA. (2006). How can we measure immunologic tolerance in humans? J Am Soc Nephrol, 17, 2652–63
   PubMed Web of Science ®Google Scholar
• Newell KA, Asare A, Kirk AD, et al. (2010). Identification of a B cell signature associated with renal transplant tolerance in humans. J Clin Investig, 120, 1836–47
   PubMed Web of Science ®Google Scholar
• Newell KA, Phippard D, Turka LA. (2011). Regulatory cells and cell signatures in clinical transplantation tolerance. Curr Opin Immunol, 23, 655–9
   PubMed Web of Science ®Google Scholar
• Olejarz W, Bryk D, Zapolska-Downar D, et al. (2014). Mycophenolic acid attenuates the tumour necrosis factor-alpha-mediated proinflammatory response in endothelial cells by blocking the MAPK/NF-kappaB and ROS pathways. Euro J Clin Invest, 44, 54–64
   PubMed Web of Science ®Google Scholar
• Perfetto SP, Chattopadhyay PK, Roederer M. (2004). Seventeen-colour flow cytometry: Unravelling the immune system. Nat Rev Immunol, 4, 648–55
   PubMed Web of Science ®Google Scholar
• Piazza A, Poggi E, Borrelli L, et al. (2011). Impact of donor-specific antibodies on chronic rejection occurrence and graft loss in renal transplantation: Posttransplant analysis using flow cytometric techniques. Transplantation, 71, 1106–12
   Google Scholar
• Pietroni V, Toscano A, Citterion F. (2013). Donor-specific antibody in solid organ transplantation: Where are we? Inter Trends Immun, 1, 5–7
   Google Scholar
• Puig-Pey I, Bohne F, Benitez C, et al. (2010). Characterization of gammadelta T cell subsets in organ transplantation. Transplant Inter, 23, 1045–55
   PubMed Web of Science ®Google Scholar
• Racusen LC, Haas M. (2006). Antibody-Mediated Rejection in Renal Allograft: Lessons from Pathology. Clin J Am Soc Nephrol, 1, 415–20
   PubMed Web of Science ®Google Scholar
• Riquelme P, Tomiuk S, Kammler A, et al. (2013). IFN-gamma-induced iNOS expression in mouse regulatory macrophages prolongs allograft survival in fully immunocompetent recipients. Mol Ther, 21, 409–22
   PubMed Web of Science ®Google Scholar
• Roat E, De Biasi S, Bertoncelli L, et al. (2012). Immunological advantages of everolimus versus cyclosporin A in liver-transplanted recipients, as revealed by polychromatic flow cytometry. Cytomet Pt A, 81, 303–11
   PubMed Web of Science ®Google Scholar
• Sagoo P, Perucha E, Sawitzki B, et al. (2010). Development of a cross-platform biomarker signature to detect renal transplant tolerance in humans. J Clin Investig, 120, 1848–61
   PubMed Web of Science ®Google Scholar
• Sato T, Fujieda M, Maeda A, et al. (2008). Monitoring of Epstein-Barr virus load and killer T cells in pediatric renal transplant recipients. Clin Nephrol, 70, 393–403
   PubMed Web of Science ®Google Scholar
• Sawitzki B, Schlickeiser S, Reinke P, Volk, HD. (2011). Monitoring tolerance and rejection in organ transplant recipients. Biomarkers, 16, S42–50
   PubMed Web of Science ®Google Scholar
• Scornik J. (1995). Detection of alloantibodies by flow cytometry: Relevance to clinical transplantation. Cytometry, 22, 259–63
   PubMedGoogle Scholar
• Shen J, Townsend R, You X, et al. (2014). Pharmacokinetics, pharmacodynamics, and immunogenicity of belatacept in adult kidney transplant recipients. Clin Drug Invest, 34, 117–26
   PubMed Web of Science ®Google Scholar
• Snanoudj R, Zuber J, Legendre C. (2010). Co-stimulation blockade as a new strategy in kidney transplantation: Benefits and limits. Drugs, 70, 2121–31
   PubMed Web of Science ®Google Scholar
• Streitz M, Miloud T, Kapinsky M, et al. (2013). Standardization of whole blood immune phenotype monitoring for clinical trials: Panels and methods from the ONE study. Transplant Res, 2, 17
   PubMedGoogle Scholar
• Stuart FP. (2003). Overview of living and deceased organ donors, immunosuppression and outcomes, Vol 1, 2nd ed. Georgetown, TX: Landes Bioscience
   Google Scholar
• Sund F, Lidehall AK, Claesson K, et al. (2010). CMV-specific T-cell immunity, viral load, and clinical outcome in seropositive renal transplant recipients: A pilot study. Clin Transplant, 24, 401–9
   PubMed Web of Science ®Google Scholar
• Tait B, Hudson F, Cantwell, L, et al. (2009). Review Article: Luminex Technology for HLA Antibody Detection in Organ Transplantation. Nephrology, 14, 247–54
   PubMed Web of Science ®Google Scholar
• Tang Q, Bluestone JA, Kang SM. (2012). CD4(+)Foxp3(+) regulatory T cell therapy in transplantation. J Mol Cell Biol, 4, 11–21
   PubMed Web of Science ®Google Scholar
• Thaunat O, Granja AG, Barral P, et al. (2012). Asymmetric segregation of polarized antigen on B cell division shapes presentation capacity. Science, 335, 475–9
   PubMed Web of Science ®Google Scholar
• Trzonkowski P, Debska-Slizien A, Jankowska M, et al. (2010). Immunosenescence increases the rate of acceptance of kidney allotransplants in elderly recipients through exhaustion of CD4+ T-cells. Mechanisms of ageing and development, 131, 96–104
   PubMed Web of Science ®Google Scholar
• Trzonkowski P, Zilvetti M, Chapman S, et al. (2008). Homeostatic repopulation by CD28-CD8+ T cells in alemtuzumab-depleted kidney transplant recipients treated with reduced immunosuppression. Amer J Transplant, 8, 338–47
   PubMed Web of Science ®Google Scholar
• Trzonkowski P, Zilvetti M, Friend P, Wood KJ. (2006). Recipient memory-like lymphocytes remain unresponsive to graft antigens after CAMPATH-1H induction with reduced maintenance immunosuppression. Transplantation, 82, 1342–51
   PubMed Web of Science ®Google Scholar
• Tsai PC, Hernandez-Ilizaliturri FJ, Bangia N, et al. (2012). Regulation of CD20 in rituximab-resistant cell lines and B-cell non-Hodgkin lymphoma. Clin Cancer Res, 18, 1039–50
   PubMed Web of Science ®Google Scholar
• Vafadari R, Hesselink DA, Cadogan MM, et al. (2012). Inhibitory effect of tacrolimus on p38 mitogen-activated protein kinase signaling in kidney transplant recipients measured by whole-blood phosphospecific flow cytometry. Transplantation, 93, 1245–51
   PubMed Web of Science ®Google Scholar
• van Es A, Baldwin WM, Oljans PJ, et al. (1984). Expression of HLA-DR on T lymphocytes following renal transplantation, and association with graft-rejection episodes and cytomegalovirus infection. Transplantation, 37, 65–9
   PubMed Web of Science ®Google Scholar
• Viklicky O, Krystufkova E, Brabcova I, et al. (2013). B-cell-related biomarkers of tolerance are up-regulated in rejection-free kidney transplant recipients. Transplantation, 95, 148–54
   PubMed Web of Science ®Google Scholar
• Villard, J. (2011). The role of natural killer cells in human solid organ and tissue transplantation. J Inn Immun, 3, 395–402
   PubMed Web of Science ®Google Scholar
• Vincenti F, Larsen C, Durrbach A, et al. (2005). Costimulation blockade with belatacept in renal transplantation. New Engl J Med, 353, 770–81
   PubMed Web of Science ®Google Scholar
• Watson M, Chow S, Barsyte D, et al. (2013). The study of epigenetic mechanisms based on the analysis of histone modification patterns by flow cytometry. Cytom Pt A, e5, 78–87
   Google Scholar
• Westall GP, Mifsud NA, Kotsimbos T. (2008). Linking CMV serostatus to episodes of CMV reactivation following lung transplantation by measuring CMV-specific CD8+ T-cell immunity. Amer J Transplant, 8, 1749–54
   PubMed Web of Science ®Google Scholar
• Wiebe C, Gibson IW, Blydt-Hansen TD, et al. (2012). Evolution and clinical pathologic correlations of de novo donor-specific HLA antibody post kidney transplant. Amer J Transplant, 12, 1157–67
   PubMed Web of Science ®Google Scholar
• Wood KJ, Bushell A, Hester J. (2012). Regulatory immune cells in transplantation. Nat Rev Immunol, 12, 417–30
   PubMed Web of Science ®Google Scholar
• Xavier PD, Lema GL, Magalhaes MC, et al. (2014). Flow cytometry assessment of graft-infiltrating lymphocytes can accurately identify acute rejection in kidney transplants. Clin Transplant, 28, 177–83
   PubMed Web of Science ®Google Scholar
• Yabu J, Higgins JP, Chen G, et al. (2011). C1q-Fixing human leukocyte antigen antibodies are specific for predicting transplant glomerulopathy and late graft failure after kidney transplantation. Transplantation, 91, 342–7
   PubMed Web of Science ®Google Scholar
• Zeevi A, Lunz JG, Shapiro R, et al. (2009). Emerging role of donor-specific anti-human leukocyte antigen antibody determination for clinical management after solid organ transplantation. Human Immunol, 70, 645–50
   PubMed Web of Science ®Google Scholar
• Zhao W, Minderman H, Russell MW. (2014). Identification and characterization of intestinal antigen-presenting cells involved in uptake and processing of a nontoxic recombinant chimeric mucosal immunogen based on cholera toxin using imaging flow cytometry. Clin Vacc Immunol, 21, 74–84
   PubMedGoogle Scholar
• Zhao Z, Wang L, Yang C, et al. (2013). Soluble FGL2 induced by tumor necrosis factor-alpha and interferon-gamma in CD4+ T cells through MAPK pathway in human renal allograft acute rejection. J Surg Res, 184, 1114–22
   PubMed Web of Science ®Google Scholar