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ORIGINAL ARTICLE

Isolation and characterization of antimicrobial proteins produced by a potential probiotic strain of human Lactobacillus rhamnosus 231 and its effect on selected human pathogens and food spoilage organisms

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Pages 211-220 | Received 20 Nov 2008, Accepted 15 Oct 2009, Published online: 26 Dec 2009

References

  • Servin AL. Antagonistic activities of lactobacilli and bifido-bacteria against microbial pathogens. FEMS Microbiol Rev 2004;28:405–40.
  • Felley C, Michetti P. Probiotics and Helicobacter pylori. Best Pract Res Clin Gastroenterol 2003;17:785–91.
  • Fuller R. A review: probiotics in man and animals. J Appl Bacteriol 1989;66:365–78.
  • Commane D, Hughes R, Shortt C, Rowland I. The potential mechanisms involved in the anticarcinogenic action of probiotics. Mutat Res 2005;591:276–89.
  • Ljungh Å, Wadstrom T. Lactic acid bacteria as probiotics. Curr Issues Intest Microbiol 2006;7:73–89.
  • Lorca GL, Wadstrom T, Valdez GF, Ljungh Å. Lactobacillus acidophilus autolysins inhibit Helicobacter pylori in vitro. Curr Microbiol 2001;42:39–44.
  • Ashar MN, Prajapati JB. Verification of hypocholesterolemic effect of fermented milk on human subjects with different cholesterol level. Folia Microbiol 2000;45:263–8.
  • Lebeer S, Vanderleyden J, De Keersmaecker SCJ. Genes and molecules of lactobacilli supporting probiotic action. Microbiol Mol Biol Rev 2008;72:728–64.
  • Khedekar CD. Characterization of human strains of Lacto-bacillus acidophilus isolates for their suitability in preparation of milk beverage and their antibacterial cum therapeutic ability. MSc (Dairy Microbiology) thesis, Gujarat Agriculture University, Anand, 1988.
  • De Man JC, Rogosa M, Sharpe ME. A medium for the cultivation of Lactobacilli. J Appl Bacteriol 1960;23:130–5.
  • Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual, 3rd. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. 2001. p. 5.4–5.13.
  • Jacobsen CN, Rosenfeldt Nielsen V, Hayford AE, Moller PL, Michaelsen KF, Paerrgaard A, . Screening of probiotic activities of 47 strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of 5 selected strains in humans. Appl Environ Microbiol 1999;65:4949–56.
  • British Standards. Methods for the estimation and differentiation of nisin in processed cheese. 1974;BS:4020.
  • Bradford MB. A rapid and sensitive method for the quantitation of microgram quantities of protein, utilizing the principle of protein-dye binding. Anal Biochem 1976;72: 248–54.
  • Deraz SF, Karlsson EN, Hedstrom M, Andersson MM, Mattiasson B. Purification and characterization of acidocin D 20079, a bacteriocin produced by Lactobacillus acidophilus DSM 20079. J Biotechnol 2005;117:343–54.
  • Schagger H, von Jagow G. Tricine-SDS-PAGE for separation of 1-100 kDa proteins. Anal Biochem 1987;166: 368–79.
  • Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual, 3rd, Vol. 3:A. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. 2001. p. 8.46–8.47.
  • Tomer K, Merav Y, Halevy G, Roni S. Immunodetection of the Bacteriocin Lacticin RM: analysis of the influence of temperature and Tween 80 on its expression and activity. Appl Environ Microbiol 2004;70:2098–104.
  • Doerner KC, White BA. Detection of glycoprotein separated by nondenaturing polyacrylamide gel electrophoresis using the periodic acid-Schiff stain. Anal Biochem 1990;187: 147–50.
  • Parente E, Brienza C, Moles M, Ricciardi A. A comparison of methods for the measurement of bacteriocin activity. J Microbiol Methods 1994;22:95–108.
  • Chou LS, Weimer B. Isolation and characterization of acid-and bile-tolerant isolates from strains of Lactobacillus acidophilus. J Dairy Sci 1999;82:23–31.
  • Lorca G, Font de Valdez G, Ljungh Å. Characterization of the protein-synthesis dependent acid tolerance response in Lactobacillus acidophilus. J Mol Microbiol Biotechnol 2002;4:59–65.
  • Havenaar R, Ten Brink B, Huis in't Veld JHJ. Selection of strains for probiotic use. Fuller R, Probiotics. The scientific basis. London: Chapman and Hall. 1992. p. 209–24.
  • Chateau N, Deschamps AM, Sassi H. Heterogeneity of bile salts resistance in the Lactobacillus isolates of a probiotic consortium. Lett Appl Microbiol 1994;18:42–4.
  • Bernet-Camard MF, Lievin V, Brassart D, Neeser JR, Servin AL, Hudault S. The human Lactobacillus acidophilus strain LA1 secretes a nonbacteriocin antibacterial substance(s) active in vitro and in vivo. Appl Environ Microbiol 1997;63: 2747–53.
  • Hudault S, Lievin V, Bernet-Camard MF, Servin AL. Antagonistic activity exerted in vitro and in vivo by Lactobacillus casei (strain GG) against Salmonella typhimurium C5 infection. Appl Environ Microbiol 1997;63:513–18.
  • Verdenelli MC, Ghelfi F, Silvi S, Orpianesi C, Cecchini C, Cresci A. Probiotic properties of Lactobacillus rhamnosus and Lactobacillus paracasei isolated from human faeces. Eur J Nutr 2009;48:355–63.
  • Silva M, Jacobus N, Deneke C, Gorbach SL. Antimicrobial substance from a human Lactobacillus strain. Antimicrob Agents Chemother 1987;31:1231–3.
  • De Klerk HC, Smit JA. Properties of a Lactobacillus fermenti. J Gen Microbiol 1967;48:309–16.
  • Muriana PM, Klaenhammer TR. Purification and partial characterization of lacticin F, a bacteriocin produced by Lactobacillus acidophilus 11088. Appl Environ Microbiol 1991;1:114–21.
  • Kim TS, Hu JW, Yu MA, Cheigh CI, Kim KN, Hwang JK, . Antagonism of Helicobacter pylori by bacteriocins of lactic acid bacteria. J Food Protect 2003;66:3–12.