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Original Article

Immunogold Labelling of Porphyromonas gingivalis in Pure Culture and in Apical Border Plaque

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Pages 181-191 | Received 19 Feb 1990, Published online: 11 Jul 2009

References

  • Adlam C, Knights J M, Mugridge A, Lindon J C, Baker P RW, Beesley J E, Spacey B, Craig G R, Nagy C K. Purification, characterization and immunological properties of the serotype-specific capsular polysaccharide of Pasteurella haemolytica, (serotype A) organisms. Journal of General Microbiology 1984; 130: 2415–2426
  • Beesley J E, Adlam C. The protein A-gold technique: a comparison between the staining mechanisms between methacrylate and cryosections. Proceedings of the 10th International Congress of Electron Microcsopy 1982; 3: 265–266
  • Bonta Y, Zambon J J, Genco R J, Neiders M E. Rapid identification of periodontal pathogens in subgingival plaque: comparison of indirect immunofluorescence microscopy with bacterial culture for detection of Actinobacillus actinomycetemcomitans. Journal of Dental Research 1985; 64: 793–798
  • Bush M S, Challacombe S J, Newman H N. A method for the identification of Streptococcus mutans, in gingival margin plaque for immunofluorescence. Caries Research 1990; 24: 23–29
  • Christersson L A, Slots J, Zambon J J, Genco J J. Transmission and colonization of Actinobacillus actinomycetemcomhans, in localized juvenile periodontitis patients. Journal of Periodontology 1985; 56: 127–131
  • Collins P A, Gerencser M A, Slack J M. Enumeration and identification of actinomyceteae in human dental calculus using fluorescent antibody technique. Archives of Oral Biology 1973; 18: 145–153
  • Czerkinsky C, Rees A S, Bergmenier L A, Challacombe S J. The detection and specificity of class specific antibodies to whole bacterial cells using a solid phase radio-immunoassay. Clinical and Experimental Immunology 1983; 53: 192–200
  • De Mey J. Colloidal gold probes in immunocytochemistry. Immunocytochemistry. Practical Applications in Pathology and Biology, J M Polak, S Van Noorden. Wright, Bristol 1983; 115–145
  • Garzon S, Bendayan M, Kurstak E. Ultrastructural localization of viral antigens using protein A-gold technique. Journal of Virological Methods 1982; 5: 67–73
  • Gelderblom H, Pauli G, Schauble H, Ludwig H. Detection of a cross-reacting antigen between herpes simplex virus type I and bovine herpes mammilitis virus by immunoelectron microscopy. Proceedings of the 7th European Congress of Electron Microscopy 1980; 2: 482–483
  • Handley P S, Carter P L, Wyatt J E, Hesketh L M. Surface structures (peritrichous fibrils and tufts of fibrils) found on Streptococcus sanguis, strains may be related to their ability to coaggregate with other genera. Infection and Immunity 1985; 47: 217–227
  • Hughes F J, Auger D W, Smales F C. Investigation of the distribution of cementum-associated lipopolysaccharides in periodontal disease by scanning electron microscope histochemistry. Journal of Periodontal Research 1988; 23: 100–106
  • Jacob E, Allen A L, Nauman R K. Detection of oral anaerobic spirochaetes in dental plaque by the indirect fluorescent-antibody technique. Journal of Clinical Microbiology 1979; 10: 934–936
  • Lai C H, Listgarten M A, Rosan B. Immunoelectron microscopic identification and localization of Streptococcus sanguis, with peroxidase-labelled antibody: localization of Streptococcus sanguis, in intact dental plaque. Infection and Immunity 1975; 11: 200–210
  • Lai C H, Listgarten M A. Immune labelling of certain strains of Actinomyces naeslundii, and Actinomyces viscosus, by fluorescence and electron microscopy. Infection and Immunity 1979; 25: 1016–1028
  • Lamonde C, Mouton C. Lectin-binding sites and adhesin for erythrocyte membranes on Bacteroides gingivalis. Journal of Dental Research 1986; 65: 737, Absts No 108
  • Mouton C, Lamonde C. Immunogold electron microscopy of surface antigens of oral bacteria. Canadian Journal of Microbiology 1984; 30: 1008–1013
  • Mayrond D, Holt S C. Biology of asaccharolytic black pigmented Bacteroides, species. Microbiological Reviews 1988; 54: 134–152
  • Nakane P K, Pierce G B. Enzyme-labelled antibodies for light and electron miroscopic localization of tissue antigens. Journal of Cell Biology 1979; 33: 307–318
  • Newman G R, Hobot J A. Modern acrylics for post-embedding immunostaining techniques. Journal of Histochemistry and Cytochemistry 1987; 35(9)971–981
  • Okuda K, Fukumoto Y, Takazoe I. Enumeration of cultivable black-pigmented Bacteroides, species in human subgingival dental and fecal samples. Oral Microbiology and Immunology 1988; 3: 28–31
  • Raybould I JG, Beesley J E, Chandler S. Ultrastructural localization of characterized antigens of Brucella abortus, and distribution among different biotypes. Infection and Immunity 1981; 32: 318–322
  • Roth J. The Protein A-gold (pAg) technique—a qualitative and quantitative approach for antigen localization of thin sections. Techniques for Immunocytochemistry, G R Bullock, P Petrusz. Academic Press, London 1982; Vol 1: 107–133
  • Silva M T, Sousa J CF. Ultrastructure of the cell wall and cytoplasmic membrane of Gramnegative bacteria with different fixation techniques. Journal of Bacteriology 1973; 113: 953–962
  • Simonson L G, Rouse R F, Bockowski S W. Monoclonal antibodies that recognize a specific surface antigen of Treponema denticola. Infection and Immunity 1988; 56: 671–681
  • Slack J M, Landfried S, Gerencser M A. Identification of Actinomyces, and related bacteria in dental calculus by the fluorescent antibody technique. Journal of Dental Research 1971; 50: 78–82
  • Slots J, Hafstrom C, Rosling B, Dahlen G. Detection of Actinobacillus actinomycetemcomitans, and Bacteroides gingivalis, in subgingival smears by the indirect fluorescent-antibody technique. Journal of Periodontal Research 1985; 20: 613–620
  • Takamiya H, Batsford S, Gelderblom H, Vogt A. Immuno-electron microscopic localization of lipopolysaccharide antigens on ultrathin sections of Salmonella typhimurium. Journal of Bacteriology 1979; 140: 261–266
  • Varndell I M, Polak J M. Double immunostaining procedures: techniques and applications. Immunolabelling for Electron Microscopy, 1st edn., J M Polak, I M Varndell. Elsevier, Amsterdam 1984; 155–177
  • Walker P D, Short J, Thomson R O, Roberts D S. The fine structure of fusiform nodosus with special reference to the location of antigens associated with disease: prevalence in patient groups and distribution of biotypes and serotypes within families. Journal of Periodontology 1973; 54: 707–711
  • Wyatt J E, Handley P S. The location of lipteichoic acid on the surface of 5. sanguis, and S. mitior, using immunogold labelling. Journal of Dental Research 1986; 65: 490
  • Wyatt J E, Russell R RB, Handley P S. Detection of antigen B in oral streptococci with known surface structure. Journal of Dental Research 1986; 65: 735
  • Zambon J J, Reynolds H S, Chen P, Genco R J. Rapid identification of periodontal pathogens in subgingival dental plaque: comparison of indirect immunofluorescence microscopy with bacterial culture for the detection of B. gingivalis. Journal of Periodontology 1985; 56((suppl))32–40