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Research Article

An experimental protocol for the fractionation and 2DE separation of HeLa and A-253 cell lysates suitable for the identification of the individual antigenic proteome in Sjögren's syndrome

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Pages 652-663 | Received 12 Dec 2010, Accepted 31 May 2011, Published online: 30 Aug 2011

References

  • Jonsson R, Bowman S, Gordon TP. 2005. Arthritis and allied conditions – a textbook of rheumatology. Philadelphia, PA: Lippincott Williams & Wilkins1681–1705.
  • Venables PJ, Smith PR, Maini RN. Purification and characterization of the Sjögren's syndrome A and B antigens. Clin Exp Immunol. 1983; 54 3: 731–738.
  • Ben-Chetrit E, Chan EK, Sullivan KF, Tan EM. A 52-kD protein is a novel component of the SS-A/Ro antigenic particle. J Exp Med. 1988; 167 5: 1560–1571.
  • Vitali C, Bombardieri S, Jonsson R, Moutsopoulos HM, Alexander EL, Carsons SE, Daniels TE, Fox PC, Fox RI, Kassan SS, Pillemer SR, Talal N, Weisman MH. Classification criteria for Sjögren's syndrome: A revised version of the European criteria proposed by the American-European Consensus Group. Ann Rheum Dis. 2002; 61 6: 554–558.
  • Jonsson R, Nginamau E, Szyszko E, Brokstad KA. Role of B cells in Sjögren's syndrome – from benign lymphoproliferation to overt malignancy. Front Biosci. 2007; 12:2159–2170.
  • Plotz PH. The autoantibody repertoire: Searching for order. Nat Rev Immunol. 2003; 3 1: 73–78.
  • Suber T, Rosen A. Apoptotic cell blebs: Repositories of autoantigens and contributors to immune context. Arthritis Rheum. 2009; 60 8: 2216–2219.
  • Casciola-Rosen LA, Anhalt G, Rosen A. Autoantigens targeted in systemic lupus erythematosus are clustered in two populations of surface structures on apoptotic keratinocytes. J Exp Med. 1994; 179 4: 1317–1330.
  • Gordon TP, Kinoshita G, Cavill D, Keech C, Farris A, Kaufman K, McCluskey J, Purcell A. Restricted specificity of intermolecular spreading to endogenous La (SS-B) and 60 kDa Ro (SS-A) in experimental autoimmunity. Scand J Immunol. 2002; 56 2: 168–173.
  • Anderton SM. Post-translational modifications of self antigens: Implications for autoimmunity. Curr Opin Immunol. 2004; 16 6: 753–758.
  • Frisoni L, McPhie L, Colonna L, Sriram U, Monestier M, Gallucci S, Caricchio R. Nuclear autoantigen translocation and autoantibody opsonization lead to increased dendritic cell phagocytosis and presentation of nuclear antigens: A novel pathogenic pathway for autoimmunity?. J Immunol. 2005; 175 4: 2692–2701.
  • Tan EM, Kunkel HG. Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus. J Immunol. 1966; 96 3: 464–471.
  • Dawson LJ, Fox PC, Smith PM. Sjögren's syndrome – the non-apoptotic model of glandular hypofunction. Rheumatology (Oxford). 2006; 45 7: 792–798.
  • Dawson L, Tobin A, Smith P, Gordon T. Antimuscarinic antibodies in Sjögren's syndrome: Where are we, and where are we going?. Arthritis Rheum. 2005; 52 10: 2984–2995.
  • Koenig JA, Edwardson JM. Intracellular trafficking of the muscarinic acetylcholine receptor: Importance of subtype and cell type. Mol Pharmacol. 1996; 49 2: 351–359.
  • Stasyk T, Huber LA. Zooming in: Fractionation strategies in proteomics. Proteomics. 2004; 4 12: 3704–3716.
  • Ho E, Hayen A, Wilkins MR. Characterisation of organellar proteomes: A guide to subcellular proteomic fractionation and analysis. Proteomics. 2006; 6 21: 5746–5757.
  • Gorg A, Drews O, Luck C, Weiland F, Weiss W. 2-DE with IPGs. Electrophoresis. 2009; 30 Suppl. 1: S122–S132.
  • Almeras L, Lefranc D, Drobecq H, de Seze J, Dubucquoi S, Vermersch P, Prin L. New antigenic candidates in multiple sclerosis: Identification by serological proteome analysis. Proteomics. 2004; 4 7: 2184–2194.
  • Looi KS, Nakayasu ES, Diaz RA, Tan EM, Almeida IC, Zhang JY. Using proteomic approach to identify tumor-associated antigens as markers in hepatocellular carcinoma. J Proteome Res. 2008; 7 9: 4004–4012.
  • Tomaino B, Cappello P, Capello M, Fredolini C, Ponzetto A, Novarino A, Ciuffreda L, Bertetto O, De Angelis C, Gaia E, Salacone P, Milella M, Nistico P, Alessio M, Chiarle R, Giuffrida MG, Giovarelli M, Novelli F. Autoantibody signature in human ductal pancreatic adenocarcinoma. J Proteome Res. 2007; 6 10: 4025–4031.
  • Hjelmervik TO, Jonsson R, Bolstad AI. The minor salivary gland proteome in Sjögren's syndrome. Oral Dis. 2009; 15 5: 342–353.
  • Hu S, Wang J, Meijer J, Ieong S, Xie Y, Yu T, Zhou H, Henry S, Vissink A, Pijpe J, Kallenberg C, Elashoff D, Loo JA, Wong DT. Salivary proteomic and genomic biomarkers for primary Sjögren's syndrome. Arthritis Rheum. 2007; 56 11: 3588–3600.
  • Bjellqvist B, Ek K, Righetti PG, Gianazza E, Gorg A, Westermeier R, Postel W. Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applications. J Biochem Biophys Meth. 1982; 6 4: 317–339.
  • Gorg A, Postel W, Westermeier R, Gianazza E, Righetti PG. Gel gradient electrophoresis, isoelectric focusing and two-dimensional techniques in horizontal, ultrathin polyacrylamide layers. J Biochem Biophys Meth. 1980; 3 5: 273–284.
  • Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, Weiss W. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis. 2000; 21 6: 1037–1053.
  • Wu CC, Yates JR3rd, Neville MC, Howell KE. Proteomic analysis of two functional states of the golgi complex in mammary epithelial cells. Traffic. 2000; 1 10: 769–782.
  • Taylor RS, Wu CC, Hays LG, Eng JK, Yates JR3rd, Howell KE. Proteomics of rat liver golgi complex: Minor proteins are identified through sequential fractionation. Electrophoresis. 2000; 21 16: 3441–3459.
  • Phadke ND, Molloy MP, Steinhoff SA, Ulintz PJ, Andrews PC, Maddock JR. Analysis of the outer membrane proteome of Caulobacter crescentus by two-dimensional electrophoresis and mass spectrometry. Proteomics. 2001; 1 5: 705–720.
  • Babu GJ, Wheeler D, Alzate O, Periasamy M. Solubilization of membrane proteins for two-dimensional gel electrophoresis: Identification of sarcoplasmic reticulum membrane proteins. Anal Biochem. 2004; 325 1: 121–125.
  • McDonough J, Marban E. Optimization of IPG strip equilibration for the basic membrane protein mABC1. Proteomics. 2005; 5 11: 2892–2895.
  • Andre M, Morelle W, Planchon S, Milhiet PE, Rubinstein E, Mollicone R, Chamot-Rooke J, Le Naour F. Glycosylation status of the membrane protein CD9P-1. Proteomics. 2007; 7 21: 3880–3895.
  • Mujahid S, Pechan T, Wang C. Improved solubilization of surface proteins from Listeria monocytogenes for 2-DE. Electrophoresis. 2007; 28 21: 3998–4007.
  • Ruan Y, Wan M. An optimized procedure for solubilization, reduction, and transfer of human breast cancer membrane-enriched fraction by 2-DE. Electrophoresis. 2007; 28 18: 3333–3340.
  • Vercauteren FG, Flores G, Ma W, Chabot JG, Geenen L, Clerens S, Fazel A, Bergeron JJ, Srivastava LK, Arckens L, Quirion R. An organelle proteomic method to study neurotransmission-related proteins, applied to a neurodevelopmental model of schizophrenia. Proteomics. 2007; 7 19: 3569–3579.
  • Rabilloud T. Membrane proteins and proteomics: Love is possible, but so difficult. Electrophoresis. 2009; 30 Suppl. 1: S174–S180.
  • Braun RJ, Kinkl N, Beer M, Ueffing M. Two-dimensional electrophoresis of membrane proteins. Anal Bioanal Chem. 2007; 389 4: 1033–1045.
  • Zhu W, Leber B, Andrews DW. Cytoplasmic O-glycosylation prevents cell surface transport of E-cadherin during apoptosis. Embo J. 2001; 20 21: 5999–6007.
  • Pasquali C, Fialka I, Huber LA. Preparative two-dimensional gel electrophoresis of membrane proteins. Electrophoresis. 1997; 18 14: 2573–2581.
  • Fujiki Y, Hubbard AL, Fowler S, Lazarow PB. Isolation of intracellular membranes by means of sodium carbonate treatment: Application to endoplasmic reticulum. J Cell Biol. 1982; 93 1: 97–102.
  • Henningsen R, Gale BL, Straub KM, DeNagel DC. Application of zwitterionic detergents to the solubilization of integral membrane proteins for two-dimensional gel electrophoresis and mass spectrometry. Proteomics. 2002; 2 11: 1479–1488.
  • Rabilloud T, Gianazza E, Catto N, Righetti PG. Amidosulfobetaines, a family of detergents with improved solubilization properties: Application for isoelectric focusing under denaturing conditions. Anal Biochem. 1990; 185 1: 94–102.
  • Slomianny MC, Dupont A, Bouanou F, Beseme O, Guihot AL, Amouyel P, Michalski JC, Pinet F. Profiling of membrane proteins from human macrophages: Comparison of two approaches. Proteomics. 2006; 6 8: 2365–2375.
  • Deshusses JM, Burgess JA, Scherl A, Wenger Y, Walter N, Converset V, Paesano S, Corthals GL, Hochstrasser DF, Sanchez JC. Exploitation of specific properties of trifluoroethanol for extraction and separation of membrane proteins. Proteomics. 2003; 3 8: 1418–1424.
  • Hubert T, Vandekerckhove J, Gettemans J. Exo70-mediated recruitment of nucleoporin Nup62 at the leading edge of migrating cells is required for cell migration. Traffic. 2009; 10 9: 1257–1271.
  • Reddy RK, Mao C, Baumeister P, Austin RC, Kaufman RJ, Lee AS. Endoplasmic reticulum chaperone protein GRP78 protects cells from apoptosis induced by topoisomerase inhibitors: Role of ATP binding site in suppression of caspase-7 activation. J Biol Chem. 2003; 278 23: 20915–20924.
  • Rabut G, Lenart P, Ellenberg J. Dynamics of nuclear pore complex organization through the cell cycle. Curr Opin Cell Biol. 2004; 16 3: 314–321.
  • Ni H, Capodici J, Cannon G, Communi D, Boeynaems JM, Kariko K, Weissman D. Extracellular mRNA induces dendritic cell activation by stimulating tumor necrosis factor-alpha secretion and signaling through a nucleotide receptor. J Biol Chem. 2002; 277 15: 12689–12696.
  • Ni M, Zhou H, Wey S, Baumeister P, Lee AS. Regulation of PERK signaling and leukemic cell survival by a novel cytosolic isoform of the UPR regulator GRP78/BiP. PLoS One. 2009; 4 8: e6868.
  • Taylor RC, Coorssen JR. Proteome resolution by two-dimensional gel electrophoresis varies with the commercial source of IPG strips. J Proteome Res. 2006; 5 11: 2919–2927.
  • Galkin VE, Orlova A, Lukoyanova N, Wriggers W, Egelman EH. Actin depolymerizing factor stabilizes an existing state of F-actin and can change the tilt of F-actin subunits. J Cell Biol. 2001; 153 1: 75–86.
  • Carlsson SR, Roth J, Piller F, Fukuda M. Isolation and characterization of human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2. Major sialoglycoproteins carrying polylactosaminoglycan. J Biol Chem. 1989; 263 35: 18911–18919.
  • Fialka I, Pasquali C, Lottspeich F, Ahorn H, Huber LA. Subcellular fractionation of polarized epithelial cells and identification of organelle-specific proteins by two-dimensional gel electrophoresis. Electrophoresis. 1997; 18 14: 2582–2590.
  • Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985; 101 1: 85–95.
  • Butor C, Griffiths G, Aronson NNJr, Varki A. Co-localization of hydrolytic enzymes with widely disparate pH optima: Implications for the regulation of lysosomal pH. J Cell Sci. 1995; 108 Pt 6: 2213–2219.
  • Tobin AB, Nahorski SR. Rapid agonist-mediated phosphorylation of m3-muscarinic receptors revealed by immunoprecipitation. J Biol Chem. 1993; 268 13: 9817–9823.
  • Poulin B, Butcher A, McWilliams P, Bourgognon JM, Pawlak R, Kong KC, Bottrill A, Mistry S, Wess J, Rosethorne EM, Charlton SJ, Tobin AB. The M3-muscarinic receptor regulates learning and memory in a receptor phosphorylation/arrestin-dependent manner. Proc Natl Acad Sci USA. 2010; 107 20: 9440–9445.
  • Nielsen PA, Olsen JV, Podtelejnikov AV, Andersen JR, Mann M, Wisniewski JR. Proteomic mapping of brain plasma membrane proteins. Mol Cell Proteom. 2005; 4 4: 402–408.
  • Olsen JV, Andersen JR, Nielsen PA, Nielsen ML, Figeys D, Mann M, Wisniewski JR. HysTag – a novel proteomic quantification tool applied to differential display analysis of membrane proteins from distinct areas of mouse brain. Mol Cell Proteom. 2004; 3 1: 82–92.
  • Wilkins MR, Gasteiger E, Sanchez JC, Bairoch A, Hochstrasser DF. Two-dimensional gel electrophoresis for proteome projects: The effects of protein hydrophobicity and copy number. Electrophoresis. 1998; 19 8–9: 1501–1505.
  • Ramsby ML, Makowski GS, Khairallah EA. Differential detergent fractionation of isolated hepatocytes: Biochemical, immunochemical and two-dimensional gel electrophoresis characterization of cytoskeletal and noncytoskeletal compartments. Electrophoresis. 1994; 15 2: 265–277.
  • Flesch B, Ntambi E, Neppert J. Biotinylation: A nonradioactive method for the identification of cell surface antigens in immunoprecipitates. Electrophoresis. 1995; 16 5: 757–762.
  • Abdolzade-Bavil A, Hayes S, Goretzki L, Kroger M, Anders J, Hendriks R. Convenient and versatile subcellular extraction procedure, that facilitates classical protein expression profiling and functional protein analysis. Proteomics. 2004; 4 5: 1397–1405.
  • Adam RM, Yang W, Di Vizio D, Mukhopadhyay NK, Steen H. Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis. BMC Cell Biol. 2008; 9:30.
  • Ostrom RS, Liu X. Detergent and detergent-free methods to define lipid rafts and caveolae. Meth Mol Biol. 2007; 400:459–468.
  • Zhang LJ, Wang XE, Peng X, Wei YJ, Cao R, Liu Z, Xiong JX, Yin XF, Ping C, Liang S. Proteomic analysis of low-abundant integral plasma membrane proteins based on gels. Cell Mol Life Sci. 2006; 63 15: 1790–1804.
  • Morla A, Poirier F, Pons S, Beaulieu C, Charrier JP, Ataman-Onal Y, Glehen O, Jolivet M, Choquet-Kastylevsky G. Analysis of high molecular mass proteins larger than 150 kDa using cyanogen bromide cleavage and conventional 2-DE. Electrophoresis. 2008; 29 20: 4158–4168.
  • Kuruma H, Egawa S, Oh-Ishi M, Kodera Y, Satoh M, Chen W, Okusa H, Matsumoto K, Maeda T, Baba S. High molecular mass proteome of androgen-independent prostate cancer. Proteomics. 2005; 5 4: 1097–1112.

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