28
Views
6
CrossRef citations to date
0
Altmetric
Original Article

Insight into Screening Immunoglobulin Gene Combinatorial Libraries in a Phage Display Vector: A Tale of Two Antibodies

, , , &
Pages 73-84 | Received 08 Jul 1996, Accepted 20 Sep 1996, Published online: 07 Jul 2009

References

  • Burton D. R., Barbas C. F. Human antibodies from combinatorial libraries. Advances in Immunology 1994; 57: 191–281
  • Winter G., Griffiths A. D., Hawkins R. E., Hoogen-Boom H. R. Making antibodies by phage display technology. Ann. Rev. Immunol. 1994; 12: 433–455
  • Rapoport B., Portolano S., McLachlan S. M. Combinatorial immunoglobulin gene libraries: new insights into human organ-specific autoantibodies. Immunol. Today 1995; 16: 43–49
  • McCafferty J., Griffiths A. D., Winter G., Chiswell D. J. Phage antibodies: filamentous phage displaying antibody variable domains. Nature 1990; 348: 552–554
  • Barbas C. F., Kang A. S., Lerner R. A., Benkovic S. J. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc. Natl. Acad. Sci. USA 1991; 88: 7978–7982
  • Portolano S., McLachlan S. M., Rapoport B. High affinity, thyroid-specific human autoantibodies displayed on the surface of filamentous phage use V genes similar to other autoantibodies. J. Immunol. 1993; 151: 2839–2851
  • Dziegel M., Nielsen L. K., Andersen P. S., Blancher A., Dickmeiss E., Engberg J. Phage display used for gene cloning of human recombinant antibody against the erythrocyte surface antigen, rhesus D. J. Immunol. Methods 1995; 182: 7–19
  • Eggena M., Targan S. R., Iwanczyk L., Vidrich A., Gordon L. K., Braun J. Phage display cloning and characterization of an immunogenetic marker (perinuclear anti-neutrophil cytoplasmic antibody) in ulcerative colitis. J. Immunol. 1996; 156: 4005–4011
  • Marks C., Marks J. D. Phage libraries — a new route to clinically useful antibodies. N. Engl. J. Med. 1996; 335: 730–733
  • McLachlan S. M., Rapoport B. Genetic and epitopic analysis of thyroid peroxidase (TPO) autoantibodies: markers of the human thyroid autoimmune response. Clin. Exp. Immunol. 1995; 101: 200–206
  • McIntosh R. S., Asghar M. S., Watson P. F., Kemp E. H., Weetman A. P. Cloning and analysis of IgG kappa and IgG lambda anti-thyroglobulin autoantibodies from a patient with Hashimoto's thyroiditis. Evidence for in vivo antigen-driven repertoire selection. J. Immunol. 1996; 157: 927–935
  • Portolano S., Seto P., Chazenbalk G. D., Nagayama Y., McLachlan S., Rapoport B. A human Fab fragment specific for thyroid peroxidase generated by cloning thyroid lymphocyte-derived immunoglobulin genes in a bacteriophage lambda library. Biochem. Biophys. Res. Comm. 1991; 179: 372–379
  • Portolano S., Chazenbalk G. D., Seto P, Hutchison J. S., Rapoport B, McLachlan S. M. Recognition by recombinant autoimmune thyroid disease-derived Fab fragments of a dominant conformational epitope on human thyroid peroxidase. J. Clin. Invest. 1992; 90: 720–726
  • Chazenbalk G. D., Portolano S., Russo D., Hutchison J. S., Rapoport B, McLachlan S. M. Human organ-specific autoimmune disease: molecular cloning and expression of an autoantibody gene repertoire for a major autoantigen reveals an antigenic dominant region and restricted immunoglobulin gene usage in the target organ. J. Clin. Invest. 1993; 92: 62–74
  • Hexham J. M., Persson M. A. A., Pegg C., Burton D., Furmaniak J., Rees Smith B. Cloning and expression of human thyroglobulin autoantibody. Autoimmunity 1991; 11: 69–70
  • Hexham J. M., Furmaniak J, Pegg C., Burton D. R., Smith B. R. Cloning of a human autoimmune response: preparation and sequencing of a human anti-thyroglobulin autoantibody using a combinatorial approach. Autoimmunity 1992; 12: 135–141
  • Hexham J. M., Partridge L. J., Furmaniak J., Petersen V. B., Colls J. C. J., Pegg C. R., et al. Cloning and characterisation of TPO autoantibodies using combinatorial phage display libraries. Autoimmunity 1994; 17: 167–179
  • Portolano S, Prummel M. F., Rapoport B., McLachlan S. M. Molecular cloning and characterization of human thyroid peroxidase autoantibodies of lambda light chain type. Molec. Immunol. 1995; 32: 1157–1169
  • Jaume J. C., Portolano S., Rapoport B., McLachlan S. M. Influence of the light chain repertoire on immunoglobulin genes encoding thyroid autoantibody Fab from combinatorial libraries. Autoimmunity 1996; 24: 11–23
  • Rees Smith B., McLachlan S. M., Furmaniak J. Autoantibodies to the thyrotropin receptor. Endocv. Rev. 1988; 9: 106–121
  • Nagayama Y., Rapoport B. The thyrotropin receptor twenty five years after its discovery: new insights following its molecular cloning. Mol. Endocrinol. 1992; 6: 145–156
  • McLachlan S. M., Rapoport B. The molecular biology of thyroid peroxidase: cloning, expression and role as autoantigen in autoimmune thyroid disease. Endocr. Rev. 1992; 13: 192–206
  • McLachlan S. M., Rapoport B. Recombinant thyroid autoantigens: the keys to the pathogenesis of autoimmune thyroid disease. J. Int. Med. 1993; 234: 347–359
  • De Forteza R., Smith C. U., Amin J., McKenzie J. M., Zakarija M. Visualization of the thyrotropin receptor on the cell surface by potent autoantibodies. J. Clin. Endocrinol. Metab. 1994; 78: 1271–1273
  • Beever K., Bradbury J., Phillips D., McLachlan S. M., Pegg C., Goral A., et al. Highly sensitive assays of autoantibodies to thyroglobulin and to thyroid peroxidase. Clin. Chem. 1989; 35: 1949–1954
  • Shewring G. A., Rees Smith B. An improved radioreceptor assay for TSH receptor antibodies. Clin. Endocrinol. 1992; 17: 409–417
  • Barbas C. F., Lerner R. A. Combinatorial immuno-globulin libraries on the surface of phage (Phabs): Rapid selection of antigen-specific Fabs. Methods Comp. Methods Enzymol. 1991; 2: 119–124
  • Foti D., Kaufman K. D., Charenbalk G., Rapoport B. Generation of a biologically-active, secreted form of human thyroid peroxidase by site-directed mutagenesis. Mol. Endocrinol. 1990; 4: 786–791
  • Kaufman K. D., Foti D., Seto P., Rapoport B. Over-expression of an immunologically-intact, secreted form of human thyroid peroxidase in eukaryotic cells. Molec. Cell. Endocrinol. 1991; 78: 107–114
  • Kaufman K. D., Rapoport B., Seto P., Chazenbalk G. D., Magnusson R. P. Generation of recombinant, enzymatically-active, human thyroid peroxidase and its recognition by antibodies in the sera of patients with Hashimoto's thyroiditis. J. Clin. Invest. 1989; 84: 394–403
  • Huang G. C., Page M. J., Nicholson L. B., Collison K. S., McGregor A. M., Banga J. P. The thyrotropin hormone receptor of Graves' disease: overexpression of the extracellular domain in insect cells using recombinant baculovirus, immunoaffinity purification and analysis of autoantibody binding. J. Mol. Endocrinology 1993; 10: 127–142
  • Nagayama Y., Kaufman K. D., Seto P., Rapoport B. Molecular cloning, sequence and functional expression of the cDNA for the human thyrotropin receptor. Bio-chem. Biophys. Res. Comm. 1989; 165: 1184–1190
  • Kakinuma A., Chazenbalk G., Filetti S., McLachlan S., Rapoport B. Both the 5′ and 3′ non-coding regions of the thyrotropin receptor messenger RNA influence the level of receptor protein expression in transfected mammalian cells. Endocrinology 1996; 137: 2664–2669
  • Salacinski P. R. P., McLean C., Sykes J. E. C., Clement-Jones V. V., Lowry P. J. lodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tctrachIoro-3 alpha, 6 alphadiphenyl Glycoluril (Iodogen). Anal. Biochem. 1981; 117: 136–146
  • Nishikawa T., Costante G., Prummel M. F., McLachlan S. M., Rapoport B. Recombinant thyroid peroxidase autoantibodies can be used for epitopic ‘fingerprinting’ of thyroid peroxidase autoantibodies in the sera of individual patients. J. Clin. Endocrinol. Metab. 1994; 78: 944–949
  • Costante G., Portolano S., Nishikawa T., Jaume J. C., Chazenbalk G. D., Rapoport B., et al. Recombinant thyroid peroxidase-specific autoantibodies. II. Role of individual heavy and light chains in determining epitope recognition. Endocrinology 1994; 134: 25–30
  • Nishikawa T., Nagayama Y., Seto P., Rapoport B. Human thyroid peroxidase-myeloperoxidase chimeric molecules: tools for the study of antigen recognition by thyroid peroxidase autoantibodies. Endocrinology 1993; 133: 2496–2501
  • Nishikawa T., Rapoport B., McLachlan S. M. Exclusion of two major areas on thyroid peroxidase from the immunodominant region containing the conformational epitopes recognized by human autoantibodies. J. Clin. Endocrinol. Metab. 1994; 79: 1648–1654
  • Filetti S., Foti D., Costante G., Rapoport B. Recombinant human TSH receptor in a radioreceptor assay for the measurement of TSH receptor autoantibodies. J. Clin. Endocrinol. & Metab 1991; 72: 1096–1101
  • Nagayama Y., Wadsworth H. L., Russo D., Chazenbalk G. D., Rapoport B. Binding domains of stimulatory and inhibitory thyrotropin (TSH) receptor autoantibodies determined with chimeric TSH-lutropin/chorionic gonadotropin receptors. J. Clin. Invest. 1991; 88: 336–340
  • Nagayama Y., Wadsworth H. L., Chazenbalk G. D., Russo D., Seto P., Rapoport B. Thyrotropin-luteinizing hormone/chorionic gonadotropin receptor extracellular domain chimeras as probes for TSH receptor function. Proc. Natl. Acad. Sci. USA 1991; 88: 902–905
  • Wadsworth H. L., Chazenbalk G. D., Nagayama Y., Russo D., Rapoport B. An insertion in the human thyrotropin receptor critical for high affinity hormone binding. Science 1990; 249: 1423–1425
  • Marks J. D., Ouwehand W. H., Bye J. M., Finnem R., Gorick B. D., Voak D., et al. Human antibody fragments specific for human blood group antigens from a phage display library. Bio/Technology 1993; 11: 1145–1149
  • Vogel M., Miescher S., Biaggi C., Stadler B. M. Human anti-IgE antibodies by repertoire cloning. Eur. J. Immunol. 1994; 24: 1200–1207
  • de Kruif J., Boel E., Logtenberg T. Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions. J. Mol. Biol. 1996; 248: 97–105
  • Jespers L. S., Roberts A., Mahler S. M., Winter G., Hoogenboom H. R. Guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen. Bio/Technology 1994; 12: 899–903
  • Clackson T., Hoogenboom H. R., Griffiths A. D., Winter G. Making antibody fragments using phage display libraries. Nature 1991; 352: 624–628
  • Marks J. D., Hoogenboom H. R., Bonnert T. P., McCafferty J., Griffiths A. D., Winter G. By-passing immunization: human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 1991; 222: 581–597
  • Watkins B. A., Davis A. E., Fiorentini S., diMarzo Veronese, Reitz M. S. Evidence for distinct contributions of heavy and light chains to restriction of antibody recognition of the HIV-1 principal neutralization determinant. J. Immunol. 1996; 156: 1676–1683
  • Maenaka K., Furuta M., Tsumoto K., Watanabe K., Ueda Y., Kumagai I. A stable phage-display system using a phagemid vector: Phage display of hen egg-white lysozyme (HEL), Eschericia coli alkaline phosphatase, and anti-HEL monoclonal antibody, HyHEL 10. Biochem. Biophys. Res. Comm. 1996; 218: 682–687
  • Barbas C. F., Bain J. D., Hoekstra D. M., Lerner R. M. Semisynthetic combinatorial antibody libraries: A chemical solution to the diversity problem. Proc. Natl. Acad. Sci. USA 1992; 89: 4457–4461
  • Meulemans E. V., Slobbe R., Wasterval P., Ramaeken F. C. S., van Eys G. J. J. M. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with amonoclonal antibody. J. Mol. Biol. 1994; 244: 353–360
  • Ward R. L., Clark M. A., Lees J., Hawkins N. J. Retrieval of human antibodies from phage-display libraries using enzymatic cleavage. J. Immunol. Methods 1996; 189: 73–82

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.