Low Level Expression of Basic FGF Upregulates Bcl-2 and Delays Apoptosis, But High Intracellular Levels are Required to Induce Transformation in NIH 3T3 Cells

References

• Araki S., Shimada Y., Kaji K., Hayashi H. Apoptosis of vascular endothelial cells by fibroblast growth factor deprivation. Bioch. Biophys. Res. Comm. 1990; 168: 1194–1200
   PubMed Web of Science ®Google Scholar
• Armentano D., Yu S.-F., Kantoff P. W., von Ruden T., Anderson W. F., Gilboa E. Effect of internal viral sequences on the utility of retroviral vectors. J. Virol. 1987; 61: 1647–1650
   PubMed Web of Science ®Google Scholar
• Baldin V., Roman A. M., Bosc-Bierne I., Amalric F., Bouche G. Translocation of bFGF to the nucleus is G1 phase cell cycle specific in bovine aortic endothelial cells. The Embo Journal 1990; 9: 1511–1517
   PubMed Web of Science ®Google Scholar
• Bikfalvi A., Klein S., Pintucci G., Quarto N., Mignatti P., Rifkin D. B. Differential Modulation of cell phenotype by different molecular weight forms of basic fibroblast growth factor: Possible intracellular signaling by the high molecular weight forms. The Journal of Cell Biology 1995; 129: 233–243
   PubMed Web of Science ®Google Scholar
• Blam S. B., Mitchell R., Tischer E., Rubin J. S., Silva M., Silver S., Fiddes J. C., Abraham J. A., Aaronson S. A. Addition of growth hormone secretion signal to basic fibroblast growth factor results in cell transformation and secretion of aberrant forms of the protein. Oncogene 1988; 3: 129–136
   PubMed Web of Science ®Google Scholar
• Briozzo P., Badet J., Capony F., Pieri I., Montcourrier P., Barritault D., Rochefort H. MCF7 mammary cancer cells respond to bFGF and internalize it following its release from extracellular matrix: a permissive role of Cathepsin. D. Exp. Cell Res. 1991; 194: 252–259
   PubMedGoogle Scholar
• Brunner G., Gabrilove J., Rifkin D. B., Wilson E. L. Phospholipase C release of basic fibroblast growth factor from human bone marrow cultures as a biologically active complex with a phosphatidylinositol-anchored heparan sulfate proteoglycan. J. Cell Biology 1991; 114: 1275–1283
   PubMed Web of Science ®Google Scholar
• Bugler B., Amalric F., Prats H. Alternative initiation of translation determines cytoplasmic or nuclear localization of basic fibroblast growth factor. Molecular and Cellular Biology 1991; 11: 573–577
   PubMed Web of Science ®Google Scholar
• Burgess W. H., Maciag T. The heparin-binding (fibroblast) growth factor family of proteins. Annu. Rev. Biochem. 1989; 58: 575–606
   PubMed Web of Science ®Google Scholar
• Cepko C. L., Roberts B. E., Mulligan R. C. Construction and applications of a highly transmissible murine retrovirus shuttle vector. Cell 1984; 37: 1053–1062
   PubMed Web of Science ®Google Scholar
• Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Bioch. 1987; 162: 156–159
   PubMed Web of Science ®Google Scholar
• Couderc B., Prats H., Bayard F., Amalric F. Potential oncogenic effects of basic fibroblast growth factor requires cooperation between CUG and AUG-initiated forms. Cell Regulation 1991; 2: 709–718
   PubMedGoogle Scholar
• Dotto G. P., Moellman G., Ghosh S., Edwards M., Halaban R. Transformation of murine melanocytes by basic fibroblast growth factor cDNA and oncogenes and selective suppression of the transformed phenotype in a reconstituted cutaneous environment. J. Cell Biol. 1989; 109: 3115–3128
   PubMedGoogle Scholar
• Fallon J. F., Lopez A., Ros M. A., Savage M. P., Olwin B. B., Simandl B. K. FGF-2: apical ectodermal ridge growth signal for chick limb development. Science 1994; 264: 104–107
   PubMed Web of Science ®Google Scholar
• Fenig E., Wieder R., Paglin S., Wang H., Persaud R., Haimovitz-Friedman A., Fuks Z., Yahalom J. Basic fibroblast growth factor confers growth inhibition and MAP kinase activation in human breast cancer cells. Clinical Cancer Research 1997; 3: 135–142
   PubMedGoogle Scholar
• Florkiewicz R. Z., Sommer A. Human basic fibroblast growth factor gene encodes four polypeptides: Three initiate translation from non-AUG codons. Proc. Natl. Acad. Sci. 1989; 86: 3978–3981
   PubMed Web of Science ®Google Scholar
• Florkiewicz R. Z., Baird A., Gonzalez A.-M. Multiple forms of bFGF: Differential nuclear and cell surface localization. Growth Factors 1991; 4: 265–275
   PubMed Web of Science ®Google Scholar
• Fuks Z., Persaud R. S., Alfieri A., McLoughlin M., Ehleiter D., Schwartz J. L., Seddon A. P., Cordon-Cardo C., Haimovitz-Friedman A. Basic fibroblast growth factor protects endothelial cells against radiation-induced programmed cell death, in vitro and in vivo. Cancer Research 1994; 54: 2582–2590
   PubMed Web of Science ®Google Scholar
• Gabrilove J. L., Welte K., Lu L., Castro-Malaspina H., Moore M. A. S. Constitutive production of leukemia differentiation, colony-stimulating, erythroid burst promoting and pluripoietic factors by a human hepatoma cell line: characterization of the leukemia differentiation factor. Blood 1985; 66: 407–415
   PubMed Web of Science ®Google Scholar
• Gospodarowicz D., Massoglia S., Cheng J., Lui G.-M., Bohlen P. Isolation of bovine pituitary fibroblast growth factor purified by fast protein liquid chromatography (FPLC), Partial chemical and biological characterization. J. Cell. Physiol. 1985; 122: 323–333
   PubMed Web of Science ®Google Scholar
• Hacker G., Vaux D. L. A sticky business. Current Biology 1995; 5: 622–624
   PubMedGoogle Scholar
• Haimovitz-Friedman A., Balaban N., McLoughlin M., Ehleiter D., Michaeli J., Vlodavsky I., Fuks Z. Protein kinase C mediates basic fibroblast growth factor protection of endothelial cells against radiation-induced apoptosis. Cancer Research. 1994; 54: 2591–2597
   PubMed Web of Science ®Google Scholar
• Hengartner M. O., Horvitz H. R. Activation of C. elegans cell death protein CED-9 and amino-acid substitution in a domain conserved in Bcl-2. Nature 1994; 369: 318–320
   PubMed Web of Science ®Google Scholar
• Hotz M. A., Gong J., Traganos F., Darzynkiewicz Z. Flow cytometric detection of apoptosis: comparison of the assays of in situ DNA degradation and chromatin changes. Cytometry 1994; 15: 237–244
   PubMedGoogle Scholar
• Joseph-Silverstein J., Moscatelli D., Rifkin D. B. The development of a quantitative RIA for basic fibroblast growth factor using polyclonal antibodies against the 157 amino acid form of human bFGF. The identification of bFGF in adherent elicited murine peritoneal macrophages. J. Immunol. Methods 1988; 110: 183–192
   PubMed Web of Science ®Google Scholar
• Kandel J., Bossy-Wetzel E., Radvanyi F., Klagsbrun M., Folkman J., Hanahan D. Neovascularization is associated with a switch to the export of bFGF in the multistep development of fibrosarcoma. Cell 1991; 66: 1095–1104
   PubMed Web of Science ®Google Scholar
• Kantoff P. W., Kohn D. B., Mitsuya H., Armentano D., Sie-Berg M., Zwiebel J. A., Eglitis M. A., McLachlin J. R., Wiginton D. A., Hutton J. J., Horowitz S. D., Gilboa E., Blaese R. M., Anderson W. F. Correction of adenosine deaminase deficiency in cultured human T and B cells by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci., U.S.A. 1986; 83: 6563–6567
   PubMed Web of Science ®Google Scholar
• Lotem J., Cragoe E. J., Sachs L. Rescue from programmed cell death in leukemic and normal myeloid cells. Blood. 1991; 78: 953–960
   PubMed Web of Science ®Google Scholar
• Maciag T., Zhan X., Garfinkel S., Friedman S., Prudovsky I., Jackson A., Wessendorf J., Hu X., Gamble S., Shi J., Brown S., Tarantini F., Zimrin A. Novel mechanisms of fibroblast growth factor 1 function. Recent Progress in Hormone Research 1994; 49: 105–122
   PubMedGoogle Scholar
• Mansour S. J., Matten W. T., Hermann A. S., Candia J. M., Rong S., Fukasawa K., Vande Woude G. F., Ahn N. Transformation of mammalian cells by constitutively active MAP kinase kinase. Science 1994; 265: 966–970
   PubMed Web of Science ®Google Scholar
• Markowitz D., Goff S., Bank A. A safe packaging line for gene transfer: separating viral genes on two different plasmids. J. Virol. 1988a; 62: 1120–1124
   PubMed Web of Science ®Google Scholar
• Markowitz D., Goff S., Bank A. Construction and use of a safe and efficient amphotropic packaging cell line. Virology. 1988b; 167: 400–406
   PubMed Web of Science ®Google Scholar
• McLeskey S. W., Ding I. Y. F., Lipman M. E., Kern F. G. MDA-MB-134 breast carcinoma cells overexpress fibroblast growth factor (FGF) receptors and are growth-inhibited by FGF ligands. Cancer Res. 1994; 54: 523–530
   PubMed Web of Science ®Google Scholar
• Menzel T., Rahman Z., Calleja E., White K., Wilson E. L., Wieder R., Gabrilove J. Elevated intracellular levels of basic fibroblast growth factor correlate with stage of chronic lymphocytic leukemia and confer resistance to fludarabine. Blood 1996; 87: 1056–1063
   PubMed Web of Science ®Google Scholar
• Miller A. D., Trauber D. R., Buttimore C. Factors involved in the production of helper virus-free retrovirus vectors. Somatic Cell Mol. Genet. 1986; 12: 175–183
   PubMedGoogle Scholar
• Oltvai Z. N., Milliman C. L., Korsmeyer S. J. Bcl-2 heterodimerizes in vivo with a conserved homolog, bax, that accelerates programmed cell death. Cell 1993; 74: 609–619
   PubMed Web of Science ®Google Scholar
• Pages G., Lenormand P., L'Allemain G., Chambard J.-C, Meloche S., Pouyssegur J. Mitogen-activated protein kinases p42mapk and p44mapk are required for fibroblast proliferation. Proc. Natl. Acad. Sci. 1993; 90: 8319–8323
   PubMed Web of Science ®Google Scholar
• Pelech S. L., Sanghera J. S. Mitogen activated protein kinases: versatile transducers for cell signaling. Trends in Biochemical Sciences 1992; 17: 233–238
   PubMedGoogle Scholar
• Prats H., Kaghad M., Prats A. C., Klagsbrun M., Lelias J. M., Liauzun P., Chalon P., Tauber J. P., Amalric F., Smith J. A., Caput D. High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codons. Proc. Natl. Acad. Sci., USA 1989; 86: 1836–1840
   PubMed Web of Science ®Google Scholar
• Prats A.-C, Vagner S., Prats H., Amalric F. cis-Acting elements involved in the alternative translation initiation process of human basic fibroblast growth factor mRNA. Molecular and Cellular Biology 1992; 12: 4796–4805
   PubMed Web of Science ®Google Scholar
• Preston G. A., Lang J. E., Maronpot R. R., Barrett J. C. Regulation of apoptosis by low serum in cells of different stages of neoplastic progression: Enhanced susceptibility after loss of a senescence gene and decreased susceptibility after loss of a tumor suppressor gene. Cancer Research 1994; 54: 4214–4223
   PubMedGoogle Scholar
• Quarto N., Talarico D., Sommer A., Florkiewitz R., Basilico C., Rifkin D. B. Transformation by basic fibroblast growth factor requires high levels of expression: comparison with transformation by hst/K-fgf. Oncogene Research 1989; 5: 101–110
   PubMedGoogle Scholar
• Quarto N., Finger F. P., Rifkin D. B. The NH2-Terminal extension of high molecular weight bFGF is a nuclear targeting signal. Journal of Cellular Physiology 1991a; 147: 311–318
   PubMed Web of Science ®Google Scholar
• Quarto N., Talarico D., Florkiewicz R., Rifkin D. B. Selective expression of high molecular weight basic fibroblast growth factor confers a unique phenotype to NIH 3T3 cells. Cell Regulation 1991b; 2: 699–708
   PubMedGoogle Scholar
• Renko M., Quarto N., Morimoto T., Rifkin D. B. Nuclear and cytoplasmic localization of different basic fibroblast growth factor species. Journal of Cellular Physiology 1990; 144: 108–114
   PubMed Web of Science ®Google Scholar
• Rogelj S., Weinberg R. A., Fanning P., Klagsbbrun M. Basic fibroblast growth factor fused to a signal peptide transforms cells. Nature 1988; 331: 173–175
   PubMed Web of Science ®Google Scholar
• Rydel R. E., Greene L. A. Acidic and basic fibroblast growth factors promote stable neurite outgrowth and neuronal differentiation in cultures of PC 12 cells. J. Neuroscience 1987; 7: 3639–3653
   PubMed Web of Science ®Google Scholar
• Schweigerer L., Neufeld G., Gospodarowicz D. Basic fibroblast growth factor as a growth inhibitor for cultured human tumor cells. J. Clin. Invest. 1987; 80: 1516–1520
   PubMed Web of Science ®Google Scholar
• Sorokin A., Mohammadi M., Huang J., Schlessinger J. Internalization of fibroblast growth factor receptor is inhibited by a point mutation at tyrosine 766. The Journal of Biological Chemistry 1994; 269: 17056–17061
   PubMed Web of Science ®Google Scholar
• Southern E. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 1975; 98: 503–517
   PubMed Web of Science ®Google Scholar
• Thompson C. B. Apoptosis in the pathogenesis and treatment of disease. Science 1995; 267: 1456–1462
   PubMed Web of Science ®Google Scholar
• Vaux D. L., Cory S., Adams J. M. Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature 1988; 335: 440–442
   PubMed Web of Science ®Google Scholar
• Wagner A. J., Small M. B., Hay N. Myc-mediated apoptosis is blocked by ectopic expression of Bcl-2. Mol. Cell. Biol. 1993; 13: 2432–2440
   PubMed Web of Science ®Google Scholar
• Wiedlocha A., Falnes P. O., Madshus I. H., Sandvig K., Olsnes S. Dual mode of signal transduction by externally added acidic fibroblast growth factor. Cell 1994; 76: 1039–1051
   PubMed Web of Science ®Google Scholar
• Williams G. T. Programmed cell death: apoptosis and oncogenesis. Cell 1991; 65: 1097–1098
   PubMed Web of Science ®Google Scholar
• Wilson E. L., Rifkin D. B., Kelly F., Hannocks M.-J., Gabrilove J. L. Basic fibroblast growth factor stimulates myelopoiesis in long-term human bone marrow cultures. Blood 1991; 77: 954–960
   PubMed Web of Science ®Google Scholar
• Yin X. M., Oltvai Z. N., Korsmeyer S. J. BH1 and BH2 domains of bcl-2 are required for inhibition of apoptosis and heterodimerization with Bax. Nature 1994; 369: 321–323
   PubMed Web of Science ®Google Scholar