284
Views
9
CrossRef citations to date
0
Altmetric
PREDICTIVE ASSAY USING H2AX AND APOPTOSIS

Prediction of radiosensitivity using phosphorylation of histone H2AX and apoptosis in human tumor cell lines

, , &
Pages 587-593 | Received 23 Jun 2013, Accepted 17 Mar 2014, Published online: 08 Apr 2014

References

  • Banath JP, MacPhail SH, Olive PL. 2004. Radiation sensitivity, H2AX phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines. Cancer Res 64:7144–7149.
  • Bartkova J, Horejsi Z, Koed K, Kramer A, Tort F, Zieger K, Guldberg P, Sehested M, Nesland JM, Lukas C, Ørntoft T, Lukas J, Bartek J. 2005. DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature 434:864–870.
  • Dasika GK, Lin SCJ, Zhao S, Sung P, Tomkinson A, Lee E. 1999. DNA damage-induced cell cycle checkpoints and DNA strand break repair in development and tumorigenesis. Oncogene 18:7883–7899.
  • Fernandez-Capetillo O, Lee A, Nussenzweig M, Nussenzweig A. 2004. H2AX: The histone guardian of the genome. DNA Repair 3:959–967.
  • Guo GZ, Sasai K, Oya N, Takagi T, Shibuya K, Hiraoka M. 1998. Simultaneous evaluation of radiation-induced apoptosis and micronuclei in five cell lines. Int J Radiat Biol 73:297–302.
  • Guo GZ, Sasai K, Oya N, Shibata T, Shibuya K, Hiraoka M. 1999. A significant correlation between clonogenic radiosensitivity and the simultaneous assessment of micronucleus and apoptotic cell frequencies. Int J Radiat Biol 75:857–864.
  • Hacker G. 2000. The morphology of apoptosis. Cell and Tissue Res 301:5–17.
  • Hakem R, Harrington L. 2005. Cell death. In: Tannock IF, Hill RP, Bristow RG, Harrington L, editors. The basic science of oncology. 4th ed. New York: McGraw-Hill Companies. pp 194–204.
  • Hall EJ, Giaccia AJ. 2012a. Molecular mechanisms of DNA and chromosome damage and repair. In: Hall EJ, Giaccia AJ, editors. Radiobiology for the radiologist. 7th ed. Philadelphia: Lippincott Williams & Wilkins. pp 13–34.
  • Hall EJ, Giaccia AJ. 2012b. Cell survival curves. In: Hall EJ, Giaccia AJ, editors. Radiobiology for the radiologist. 7th ed. Philadelphia: Lippincott Williams & Wilkins. pp 35–53.
  • Kanda Y, Nishiyama Y, Shimada Y, Imamura M, Nomura H, Hiai H, Fukumoto M. 1994. Analysis of gene amplification and overexpression in human esophageal-carcinoma cell-lines. Int J Cancer 58:291–297.
  • Kobayashi J, Iwabuchi K, Miyagawa K, Sonoda E, Suzuki K, Takata M, Tauchi H. 2008. Current topics in DNA double-strand break repair. J Radiat Res 49:93–103.
  • Koch U, Höhne K, von Neubeck C, Thames HD, Yaromina A, Dahm-Daphi J, Baumann M, Krause M. 2013. Residual γH2AX foci predict local tumour control after radiotherapy. Radiother Oncol 108:434–439.
  • Kuhne M, Riballo E, Rief N, Rothkamm K, Jeggo PA, Löbrich M. 2004. A double-strand break repair defect in ATM-deficient cells contributes to radiosensitivity. Cancer Res 64:500–508.
  • Mahrhofer H, Burger S, Oppitz U, Flentje M, Djuzenova CS. 2006. Radiation induced DNA damage and damage repair in human tumor and fibroblast cell lines assessed by histone H2AX phosphorylation. Int J Radiat Oncol Biol Phys 64:573–580.
  • McBride WH, Withers HR. 2007. Biologic basis of radiation therapy. In: Halperin EC, Perez CA, Brady LW, editors. Perez and Brady's principle and practice of radiation oncology. 5th ed. Philadelphia: Lippincott Williams & Wilkins. pp 76–108.
  • Nishihira T, Hashimoto Y, Katayama M, Mori S, Kuroki T. 1993. Molecular and cellular-features of esophageal cancer-cells. J Cancer Res Clin Oncol 119:441–449.
  • Olive PL, Banath JP. 2004. Phosphorylation of histone H2AX as a measure of radiosensitivity. Int J Radiat Oncol Biol Phys 58:331–335.
  • Olive PL, Banuelos CA, Durand RE, Kim JY, Aquino-Parsons C. 2010. Endogenous and radiation-induced expression of γH2AX in biopsies from patients treated for carcinoma of the uterine cervix. Radiother Oncol 94:82–89.
  • Olive PL. 2011. Retention of γH2AX foci as an indication of lethal DNA damage. Radiother Oncol 101:18–23.
  • Runge R, Hiemann R, Wendisch M, Kasten-Pisula U, Storch K, Zöphel K, Fritz C, Roggenbuck D, Wunderlich G, Conrad K, Kotzerke J. 2012. Fully automated interpretation of ionizing radiation-induced γH2AX foci by the novel pattern recognition system AKLIDES®. Int J Radiat Biol 88:439–447.
  • Sasai K, Evans JW, Kovacs MS, Brown JM. 1994. Prediction of human cell radiosensitivity – comparison of clonogenic-assay with chromosome-aberrations scored using premature chromosome condensation with fluorescence in-situ hybridization. Int J Radiat Oncol Biol Phys 30:1127–1132.
  • Schmid TE, Zlobinskaya O, Gabriele Multhoff G. 2012. Differences in phosphorylated histone H2AX foci formation and removal of cells exposed to low and high linear energy transfer radiation. Curr Genomics 13:418–425.
  • Sedelnikova OA, Pilch DR, Redon C, Bonner WM. 2003. Histone H2AX in DNA damage and repair. Cancer Biol Ther 2:233–235.
  • Sedelnikova OA, Bonner WM. 2006. GammaH2AX in cancer cells: A potential biomarker for cancer diagnostics, prediction and recurrence. Cell Cycle 5:2909–2913.
  • Shibamoto Y, Streffer C, Fuhrmann C, Budach V. 1991. Tumor radiosensitivity prediction by the cytokinesis-block micronucleus assay. Radiat Res 128:293–300.
  • Syrios J, Sougioultzis S, Xynos ID, Kavantzas N, Kosmas C, Agrogiannis G, Griniatsos J, Karavokyros I, Pikoulis E, Patsouris ES, Tsavaris N. 2012. Survival in patients with stage IV noncardia gastric cancer – the influence of DNA ploidy and Helicobacter pylori infection. BMC Cancer 12:264.
  • Takagi T, Sasai K, Shibamoto Y, Akagi K, Oya N, Shibata T, Kim J, Hiraoka M. 1998. The influence of DNA ploidy of a human tumor cell line on the frequencies of micronuclei or chromosome aberrations after irradiation. Mutat Res 418:49–57.
  • Takahashi A, Ohnishi T. 2005. Does gammaH2AX foci formation depend on the presence of DNA double strand breaks?Cancer Lett 229:171–179.
  • Takahashi A, Mori E, Somakos GI, Ohnishi K, Ohnishi T. 2008. Heat induces gammaH2AX foci formation in mammalian cells. Mutat Res 656:88–92.
  • Taneja N, Davis M, Choy JS, Beckett MA, Singh R, Kron SJ, Weichselbaum RR. 2004. Histone H2AX phosphorylation as a predictor of radiosensitivity and target for radiotherapy. J Biol Chem 279:2273–2280.
  • West CM. 1995. Intrinsic radiosensitivity as a predictor of patient response to radiotherapy. Br J Radiol 68:827–837.
  • Yoshikawa T, Kashino G, Ono K, Watanabe M. 2009. Phosphorylated H2AX foci in tumor cells have no correlation with their radiation sensitivities. J Radiat Res 50:151–160.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.