848
Views
8
CrossRef citations to date
0
Altmetric
Research Article

Cloning, expression and purification of orthologous membrane proteins: a general protocol for preparation of the histidine sensor kinase ETR1 from different species

&
Pages 26-35 | Received 05 Sep 2011, Accepted 22 Dec 2012, Published online: 14 Mar 2012

References

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res 25(17):3389–3402.
  • Bleecker AB, Esch JJ, Hall AE, Rodríguez FI, Binder BM. 1998. The ethylene-receptor family from Arabidopsis: Structure and function. Phil Trans R Soc B 353(1374):1405–1412.
  • Bleecker AB, Kende H. 2000. Ethylene: A gaseous signal molecule in plants. Annu Rev Cell Dev Biol 16(1):1–18.
  • Calderwood DA, Shattil SJ, Ginsberg MH. 2000. Integrins and actin filaments: Reciprocal regulation of cell adhesion and signaling. J Biol Chem 275(30):22607–22610.
  • Chen YF, Etheridge N, Schaller GE. 2005. Ethylene signal transduction. Ann Bot 95(6):901–915.
  • Cove DJ, Perroud PF, Charron AJ, McDaniel SF, Khandelwal A, Quatrano RS. 2009. The moss Physcomitrella patens: A novel model system for plant development and genomic studies. Cold Spring Harb Protoc 2009; doi:10.1101/pdb.emo115.
  • Gamble RL, Coonfield ML, Schaller GE. 1998. Histidine kinase activity of the ETR1 ethylene receptor from Arabidopsis. Proc Nat Acad Sci USA 95(13):7825–7829.
  • Geourjon C, Deléage G. 1995. SOPMA: Significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments. Comput Appl Biosci 11(6):681–684.
  • Heukeshoven J, Dernick R. 1988. Improved silver staining procedure for fast staining in PhastSystem Development Unit. I. Staining of sodium dodecyl sulfate gels. Electrophoresis 9(1):28–32.
  • Hwang I, Chen HC, Sheen J. 2002. Two-component signal transduction pathways in Arabidopsis. Plant Physiol 129(2):500–515.
  • Johnson PR, Ecker JR. 1998. The ethylene gas signal transduction pathway: A molecular perspective. Annu Rev Genet 32:227–254.
  • Kimura S, Sinha N. 2008. Tomato (Solanum lycopersicum): A model fruit-bearing crop. Cold Spring Harb Protoc 2008; doi: 10.1101/pdb.emo105.
  • Kosinski M, Rinas U, Bailey J. 1992. Isopropyl-β-d-thiogalactopyranoside influences the metabolism of Escherichia coli. Appl Microbiol Biotechnol 36(6):782–784.
  • Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685.
  • Li MZ, Elledge SJ. 2007. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nat Methods 4(3):251–256.
  • Li Y, Agrawal A, Sakon J, Beitle RR. 2001. Characterization of metal affinity of green fluorescent protein and its purification through salt promoted, immobilized metal affinity chromatography. J Chromatogr A 909(2):183–190.
  • Miroux B, Walker JE. 1996. Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J Mol Biol 260(3):289–298.
  • Rath A, Glibowicka M, Nadeau VG, Chen G, Deber CM. 2009. Detergent binding explains anomalous SDS-PAGE migration of membrane proteins. Proc Nat Acad Sci USA 106(6):1760–1765.
  • Ren H, Yu D, Ge B, Cook B, Xu Z, Zhang S. 2009. High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1. PLoS One 4(2):e4509.
  • Rodríguez FI, Esch JJ, Hall AE, Binder BM, Schaller GE, Bleecker AB. 1999. A copper cofactor for the ethylene receptor ETR1 from Arabidopsis. Science 283(5404):996–998.
  • Sambrook J, Russell DW. 2006. The condensed protocols from Molecular cloning: A laboratory manual. New York: Cold Spring Harbor Laboratory Press.
  • Schaller GE, Ladd AN, Lanahan MB, Spanbauer JM, Bleecker AB. 1995. The ethylene response mediator ETR1 from Arabidopsis forms a disulfide-linked dimer. J Biol Chem 270(21):12526–12530.
  • Sreerama N, Woody RW. 2000. Estimation of protein secondary structure from circular dichroism spectra: Comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set. Anal Biochem 287(2):252–260.
  • Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Nat Acad Sci USA 76(9):4350–4354.
  • Voet-van-Vormizeele J, Groth G. 2008. Ethylene controls autophosphorylation of the histidine kinase domain in ethylene receptor ETR1. Mol Plant 1(2):380–387.
  • White SH. 2009. Biophysical dissection of membrane proteins. Nature 459(7245):344–346.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.